DPSS yellow-green 561-nm lasers for improved fluorochrome detection by flow cytometry.
AuthorsTelford W, Murga M, Hawley T, Hawley R, Packard B, Komoriya A, Haas F, Hubert C
JournalCytometry A
PubMed ID16163703
INTRODUCTION: Blue-green 488-nm laser sources are widespread in flow cytometry but suffer some drawbacks for cell analysis, including their excitation of endogenous proteins (resulting in high cellular autofluorescence) and their less-than-optimal coincidence with the excitation maxima of commonly used fluorochromes, including the phycoerythrins (PE). Longer wavelength lasers such as green ... More