A novel notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia.
AuthorsDuan Z, Li FQ, Wechsler J, Meade-White K, Williams K, Benson KF, Horwitz M,
JournalMol Cell Biol
PubMed ID14673143
Mutations in ELA2, encoding the human serine protease neutrophil elastase, cause cyclic and severe congenital neutropenia, and recent evidence indicates that the mutations alter the membrane trafficking of neutrophil elastase. These disorders feature impaired bone marrow production of neutrophils along with excess monocytes-terminally differentiated lineages corresponding to the two alternative ... More
A gene encoding a protein modified by the phytohormone indoleacetic acid.
Authors Walz Alexander; Park Seijin; Slovin Janet P; Ludwig-Müller Jutta; Momonoki Yoshie S; Cohen Jerry D;
JournalProc Natl Acad Sci U S A
PubMed ID11830675
'We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this protein undergoes rapid degradation during germination. The gene for IAP1, the most abundant protein covalently modified by IAA (iap1, GenBank accession ... More
Gata factor translation is the final downstream step in the amino acid/tor-mediated vitellogenin gene expression in the anautogenous mosquito aedes aegypti.
'Ingestion of blood is required for vector mosquitoes to initiate reproductive cycles determining their role as vectors of devastating human diseases. Nutritional signaling plays a pivotal role in regulating mosquito reproduction. Transcription of yolk protein precursor genes is repressed until mosquitoes take blood. Previously, we have shown that to signal ... More
Alternative promoters regulate transcription of the gene that encodes stem cell surface protein AC133.
AuthorsShmelkov SV, Jun L, St Clair R, McGarrigle D, Derderian CA, Usenko JK, Costa C, Zhang F, Guo X, Rafii S,
JournalBlood
PubMed ID14630820
'AC133 is a member of a novel family of cell surface proteins with 5 transmembrane domains. The function of AC133 is unknown. Although AC133 mRNA is detected in different tissues, its expression in the hematopoietic system is restricted to CD34+ stem cells. AC133 is also expressed on stem cells of ... More
Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.
AuthorsTone M, Tone Y, Adams E, Yates SF, Frewin MR, Cobbold SP, Waldmann H,
JournalProc Natl Acad Sci U S A
PubMed ID14608036
'Recently, agonist antibodies to glucocorticoid-induced tumor necrosis factor receptor (GITR) (tumor necrosis factor receptor superfamily 18) have been shown to neutralize the suppressive activity of CD4+CD25+ regulatory T cells. It was anticipated that this would be the role of the physiological ligand. We have identified and expressed the gene for ... More
The MUC3A human intestinal mucin: Initiation of transcription from a TATA-less promoter and comparison to the MUC3B amino terminus.
AuthorsGum JR, Hicks JW, Crawley SC, Dahl CM, Yang SC, Roberton AM, Kim YS,
JournalJ Biol Chem
PubMed ID12958310
'Human intestinal mucin genes MUC3A and MUC3B are members of a membrane mucin gene family residing at chromosome 7q22. In this paper, we utilized genomic and cDNA cloning to elucidate the sequence of the 5''-region of the MUC3A gene including the gene promoter and the amino terminus coding sequence. Following ... More
Identifying novel transcripts and novel genes in the human genome by using novel SAGE tags.
Authors Chen Jianjun; Sun Miao; Lee Sanggyu; Zhou Guolin; Rowley Janet D; Wang San Ming;
JournalProc Natl Acad Sci U S A
PubMed ID12213963
'The number of genes in the human genome is still a controversial issue. Whereas most of the genes in the human genome are said to have been physically or computationally identified, many short cDNA sequences identified as tags by use of serial analysis of gene expression (SAGE) do not match ... More
Mouse epididymal Spam1 (pH-20) is released in the luminal fluid with its lipid anchor.
AuthorsZhang H, Martin-Deleon PA,
JournalJ Androl
PubMed ID12514083
Previously we demonstrated that the murine sperm adhesion molecule 1 (Spam1 or PH-20) is synthesized by the epididymal epithelium, preferentially in the distal region, and is released into the luminal fluid. We also showed that whereas testicular and epididymal Spam1 have hyaluronidase activity at neutral pH, they are under different ... More
The human apolipoprotein AV gene is regulated by peroxisome proliferator-activated receptor-alpha and contains a novel farnesoid X-activated receptor response element.
AuthorsPrieur X, Coste H, Rodriguez JC,
JournalJ Biol Chem
PubMed ID12709436
The newly identified apolipoprotein AV (apoAV) gene is a key player in determining plasma triglyceride concentrations. Because hypertriglyceridemia is a major independent risk factor in coronary artery disease, the understanding of the regulation of the expression of this gene is of considerable importance. We presently characterize the structure, the transcription ... More
Mycobacterium-inducible Nramp in striped bass (Morone saxatilis).
AuthorsBurge EJ, Gauthier DT, Ottinger CA, Van Veld PA,
JournalInfect Immun
PubMed ID14977970
In mammals, the natural resistance-associated macrophage protein 1 gene, Nramp1, plays a major role in resistance to mycobacterial infections. Chesapeake Bay striped bass (Morone saxatilis) is currently experiencing an epizootic of mycobacteriosis that threatens the health of this ecologically and economically important species. In the present study, we characterized an ... More
Mastitis increases mammary mRNA abundance of beta-defensin 5, toll-like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle.
AuthorsGoldammer T, Zerbe H, Molenaar A, Schuberth HJ, Brunner RM, Kata SR, Seyfert HM,
JournalClin Diagn Lab Immunol
PubMed ID14715566
Coordination of the primary defense mechanisms against pathogens relies on the appropriate expression of pathogen recognition receptors (PRRs) triggering the early release of effector molecules of the innate immune system. To analyze the impact of this system on the counteraction of infections of the mammary gland (mastitis), we characterized the ... More
High throughput immuno-screening of cDNA expression libraries produced by in vitro recombination; exploring the Plasmodium falciparum proteome.
AuthorsKordai Sowa MP, Sharling L, Humphreys G, Cavanagh DR, Gregory WF, Fenn K, Creasey AM, Arnot DE,
JournalMol Biochem Parasitol
PubMed ID14698438
Improved Plasmodium falciparum cDNA expression libraries were constructed by combining mRNA oligo-capping with in vitro recombination and directional cloning of cDNA inserts into a plasmid vector that expresses sequences as thioredoxin fusion proteins. A novel procedure has also been developed for the rapid identification of seropositive clones on high-density filters, ... More
An isoform of ZBP-89 predisposes the colon to colitis.
AuthorsLaw DJ, Labut EM, Adams RD, Merchant JL,
JournalNucleic Acids Res
PubMed ID16517939
Alternative splicing enables expression of functionally diverse protein isoforms. The structural and functional complexity of zinc-finger transcription factor ZBP-89 suggests that it may be among the class of alternatively spliced genes. We identified a human ZBP-89 splice isoform (ZBP-89(DeltaN)), which lacks amino terminal residues 1-127 of the full-length protein (ZBP-89(FL)). ... More
Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids.
Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana, ... More
A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol.
Authors Lin Shan; Shi Qing; Nix F Brent; Styblo Miroslav; Beck Melinda A; Herbin-Davis Karen M; Hall Larry L; Simeonsson Josef B; Thomas David J;
JournalJ Biol Chem
PubMed ID11790780
S-Adenosyl-l-methionine (AdoMet):arsenic(III) methyltransferase, purified from liver cytosol of adult male Fischer 344 rats, catalyzes transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. The kinetics of production of methylated arsenicals in reaction mixtures containing enzyme, AdoMet, dithiothreitol, glutathione (GSH), and arsenite are consistent with ... More
Human calcium-sensing receptor gene. Vitamin D response elements in promoters P1 and P2 confer transcriptional responsiveness to 1,25-dihydroxyvitamin D.
AuthorsCanaff L, Hendy GN.
JournalJ Biol Chem
PubMed ID12036954
The calcium-sensing receptor (CASR), expressed in parathyroid chief cells, thyroid C-cells, and cells of the kidney tubule, is essential for maintenance of calcium homeostasis. Here we show parathyroid, thyroid, and kidney CASR mRNA levels increased 2-fold at 15 h after intraperitoneal injection of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in rats. Human thyroid ... More