Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.
AuthorsDamiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E,
JournalJ Neurosci
PubMed ID18463241
'MicroRNAs (miRNAs) are small, highly conserved molecules that have been shown to regulate the expression of genes by binding to specific target mRNAs. Dicer, an RNase III endonuclease, is essential for the production and function of mature miRNAs, and removal of Dicer has been shown to disrupt many developmental processes. ... More
Lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus Beauveria bassiana.
AuthorsWanchoo A, Lewis MW, Keyhani NO,
JournalMicrobiology
PubMed ID19608611
'The entomopathogenic fungus Beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. Carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. B. bassiana produces a ... More
Retention of function without normal disc morphogenesis occurs in cone but not rod photoreceptors.
'It is commonly assumed that photoreceptor (PR) outer segment (OS) morphogenesis is reliant upon the presence of peripherin/rds, hereafter termed Rds. In this study, we demonstrate a differential requirement of Rds during rod and cone OS morphogenesis. In the absence of this PR-specific protein, rods do not form OSs and ... More
Mutation of the COG complex subunit gene COG7 causes a lethal congenital disorder.
AuthorsWu X, Steet RA, Bohorov O, Bakker J, Newell J, Krieger M, Spaapen L, Kornfeld S, Freeze HH
JournalNat Med
PubMed ID15107842
'The congenital disorders of glycosylation (CDG) are characterized by defects in N-linked glycan biosynthesis that result from mutations in genes encoding proteins directly involved in the glycosylation pathway. Here we describe two siblings with a fatal form of CDG caused by a mutation in the gene encoding COG-7, a subunit ... More
Anti-glycosyl antibodies in lipid rafts of the enterocyte brush border: a possible host defense against pathogens.
AuthorsHansen GH, Pedersen ED, Immerdal L, Niels-Christiansen LL, Danielsen EM
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID16081758
The pig small intestinal brush border is a glycoprotein- and glycolipid-rich membrane that functions as a digestive/absorptive surface for dietary nutrients as well as a permeability barrier for pathogens. The present work was performed to identify carbohydrate-binding (lectinlike) proteins associated with the brush border. Chromatography on lactose-agarose was used to ... More
Genetic analysis of the subunit organization and function of the conserved oligomeric golgi (COG) complex: studies of COG5- and COG7-deficient mammalian cells.
AuthorsOka T, Vasile E, Penman M, Novina CD, Dykxhoorn DM, Ungar D, Hughson FM, Krieger M
JournalJ Biol Chem
PubMed ID16051600
The conserved oligomeric Golgi (COG) complex is an eight-subunit (Cog1-8) peripheral Golgi protein involved in Golgi-associated membrane trafficking and glycoconjugate synthesis. We have analyzed the structure and function of COG using Cog1 or Cog2 null Chinese hamster ovary cell mutants, fibroblasts from a patient with Cog7-deficient congenital disorders of glycosylation, ... More
Genetic rescue models refute nonautonomous rod cell death in retinitis pigmentosa.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID28468800
Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF19.
Authors
JournalElife
PubMed ID32869745
Gene therapy restores vision in rd1 mice after removal of a confounding mutation in Gpr179.
Authors
JournalNat Commun
PubMed ID25613321
Independent genomic control of neuronal number across retinal cell types.
Authors
JournalDev Cell
PubMed ID24954025
Gcn5-Mediated Histone Acetylation Governs Nucleosome Dynamics in Spermiogenesis.
Authors
JournalDev Cell
PubMed ID31761669
Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.
Authors
JournalCell Rep
PubMed ID31995746
BMP4 drives primed to naïve transition through PGC-like state.
Authors
JournalNat Commun
PubMed ID35589713
Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.
Authors
JournalAutophagy
PubMed ID32677505
Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis.
Authors
JournalTheranostics
PubMed ID33754041
A phenotypic screening platform utilising human spermatozoa identifies compounds with contraceptive activity.