Influence of P-glycoprotein inhibitors on accumulation of macrolides in J774 murine macrophages.
AuthorsSeral C, Michot JM, Chanteux H, Mingeot-Leclercq MP, Tulkens PM, Van Bambeke F
JournalAntimicrob Agents Chemother
PubMed ID12604540
'The influence of inhibitors of P-glycoprotein (verapamil [VE], cyclosporine [CY], and GF120918 [GF]) on the cell handling of macrolides (erythromycin [ERY], clarithromycin [CLR], roxithromycin [ROX], azithromycin [AZM], and telithromycin [TEL]) was examined in J774 murine macrophages. The net influx rates of AZM and TEL were increased from 2- to 3.5-fold ... More
d-Tocopherol Reduces Lipid Accumulation in Niemann-Pick Type C1 and Wolman Cholesterol Storage Disorders.
AuthorsXu M, Liu K, Swaroop M, Porter FD, Sidhu R, Finkes S, Ory DS, Marugan JJ, Xiao J, Southall N, Pavan WJ, Davidson C, Walkley SU, Remaley AT, Baxa U, Sun W, McKew JC, Austin CP, Zheng W,
JournalJ Biol Chem
PubMed ID23035117
'Niemann-Pick disease type C (NPC) and Wolman disease are two members of a family of storage disorders caused by mutations of genes encoding lysosomal proteins. Deficiency in function of either the NPC1 or NPC2 protein in NPC disease or lysosomal acid lipase in Wolman disease results in defective cellular cholesterol ... More
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
AuthorsLee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, Westaway D, Cuervo AM, Nixon RA,
JournalCell
PubMed ID20541250
Macroautophagy is a lysosomal degradative pathway essential for neuron survival. Here, we show that macroautophagy requires the Alzheimer's disease (AD)-related protein presenilin-1 (PS1). In PS1 null blastocysts, neurons from mice hypomorphic for PS1 or conditionally depleted of PS1, substrate proteolysis and autophagosome clearance during macroautophagy are prevented as a result ... More
Cytokines regulate proteolysis in major histocompatibility complex class II-dependent antigen presentation by dendritic cells.
AuthorsFiebiger E, Meraner P, Weber E, Fang IF, Stingl G, Ploegh H, Maurer D
JournalJ Exp Med
PubMed ID11304549
Endo/lysosomal proteases control two key events in antigen (Ag) presentation: the degradation of protein Ag and the generation of peptide-receptive major histocompatibility complex (MHC) class II molecules. Here we show that the proinflammatory cytokines tumor necrosis factor alpha and interleukin (IL)-1beta rapidly increase the activity of cathepsin (cat) S and ... More
Optimal transfection with the HK polymer depends on its degree of branching and the pH of endocytic vesicles.
AuthorsChen QR, Zhang L, Luther PW, Mixson AJ
JournalNucleic Acids Res
PubMed ID11884631
We have recently reported that liposomes in combination with histidine (HK)-containing polymers enhanced the expression of luciferase in transfected cells. In transformed or malignant cell lines, branched HK polymers (combined with liposome carriers) were significantly more effective than the linear HK polymer in stimulating gene expression. In the current study, ... More
Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages.
AuthorsYanagawa M, Tsukuba T, Nishioku T, Okamoto Y, Okamoto K, Takii R, Terada Y, Nakayama KI, Kadowaki T, Yamamoto K
JournalJ Biol Chem
PubMed ID17095504
Cathepsin E, an endolysosomal aspartic proteinase predominantly expressed in cells of the immune system, has an important role in immune responses. However, little is known about the precise roles of cathepsin E in this system. Here we report that cathepsin E deficiency (CatE(-/-)) leads to a novel form of lysosome ... More
Lysosomal basification and decreased autophagic flux in oxidatively stressed trabecular meshwork cells: implications for glaucoma pathogenesis.
Authors
JournalAutophagy
PubMed ID23360789
The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion.
Authors
JournalAutophagy
PubMed ID30681394
ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H+-ATPases is essential for brain development in humans and mice.
Authors
JournalNat Commun
PubMed ID33833240
Presenilin 1 phosphorylation regulates amyloid-β degradation by microglia.
Authors
JournalMol Psychiatry
PubMed ID32792660
Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease.
Authors
JournalCell Rep
PubMed ID34469722
Loss of PKD1/polycystin-1 impairs lysosomal activity in a CAPN (calpain)-dependent manner.
Authors
JournalAutophagy
PubMed ID32967521
Concanamycin A counteracts HIV-1 Nef to enhance immune clearance of infected primary cells by cytotoxic T lymphocytes.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID32900948
NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification.
Authors
JournalEMBO J
PubMed ID34435379
A broad-spectrum antibiotic, DCAP, reduces uropathogenic Escherichia coli infection and enhances vorinostat anticancer activity by modulating autophagy.