A new fluorescence-based, hydrophobic photolabeling technique for analyzing membrane-associated proteins.
AuthorsHess D, Isenberg G
JournalFEBS Lett
PubMed ID10094472
We introduce a new, fluorescent and photoactivatable fatty acid derivative (SANU) for hydrophobic labelling of membrane-bound proteins. The technique allows fast and highly sensitive screening of hydrophobically inserting proteins analyzed by SDS-PAGE with a detection limit below 0.1 pmol. A reliable calculation of labelling efficiencies is achieved by simultaneous densitometry ... More