Characterization of postsynaptic Ca2+ signals at the Drosophila larval NMJ.
AuthorsDesai SA, Lnenicka GA,
JournalJ Neurophysiol
PubMed ID21593388
'Postsynaptic intracellular Ca(2+) concentration ([Ca(2+)](i)) has been proposed to play an important role in both synaptic plasticity and synaptic homeostasis. In particular, postsynaptic Ca(2+) signals can alter synaptic efficacy by influencing transmitter release, receptor sensitivity, and protein synthesis. We examined the postsynaptic Ca(2+) transients at the Drosophila larval neuromuscular junction ... More
Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions.
AuthorsChu YH, Lees WJ, Stassinopoulos A, Walsh CT
JournalBiochemistry
PubMed ID8075061
'We have developed a new method utilizing affinity capillary electrophoresis (ACE) for the determination of binding stoichiometries in biochemical systems. Using the same concentration of a ligand in the sample and the electrophoresis buffer, the appearance of an inverted peak corresponding to the free ligand in the resulting electropherogram provides ... More
Connectivity of neurons in identified auditory circuits studied with transport of dextran and microspheres plus intracellular injection of lucifer yellow.
AuthorsOliver DL, Beckius GE, Ostapoff EM
JournalJ Neurosci Methods
PubMed ID7527475
The components of a neural circuit are usually distinguished in separate experiments to identify long connections, presynaptic, and postsynaptic components. We describe a procedure to visualize these components in the same experiment. Neurons in the inferior colliculus the axons of which project to the medial geniculate body were identified by ... More
Visualization of neurons filled with biotinylated-lucifer yellow following identification of efferent connectivity with retrograde transport.
AuthorsHill SJ, Oliver DL
JournalJ Neurosci Methods
PubMed ID8459723
A new method is described that allows identification of neurons by retrograde transport, intracellular injection of biotin-labeled Lucifer Yellow, and a histochemical reaction of the biotin. Cells in the inferior colliculus that project to the ipsilateral thalamus are identified by injection of rhodamine-labeled latex beads into the medial geniculate body ... More
Comparative morphology of three types of projection-identified pyramidal neurons in the superficial layers of cat visual cortex.
AuthorsMatsubara JA, Chase R, Thejomayen M
JournalJ Comp Neurol
PubMed ID8866848
The morphology and dendritic organization of corticocortical neurons in the superficial layers of area 18 that project to area 17 were studied by intracellular injection of lucifer yellow in the fixed-slice preparation. This corticocortical population contains primarily standard pyramidal cells, but occasional nonpyramidal, modified, fusiform, star, and inverted pyramidal cells ... More