LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor.
AuthorsZelcer N, Hong C, Boyadjian R, Tontonoz P,
JournalScience
PubMed ID19520913
'Cellular cholesterol levels reflect a balance between uptake, efflux, and endogenous synthesis. Here we show that the sterol-responsive nuclear liver X receptor (LXR) helps maintain cholesterol homeostasis, not only through promotion of cholesterol efflux but also through suppression of low-density lipoprotein (LDL) uptake. LXR inhibits the LDL receptor (LDLR) pathway ... More
Specific binding of human low-density lipoprotein to the surface of schistosomula of Schistosoma mansoni and ingestion by the parasite.
AuthorsBennett MW, Caulfield JP
JournalAm J Pathol
PubMed ID2024706
'Low-density lipoproteins (LDL) may be important in human schistosomiasis because LDL bound to the surface of the parasite inhibits the binding of anti-schistosomal antibodies. Low-density lipoproteins also may serve as a source of lipids for the parasite membrane synthesis. Here LDL fluorescently labeled with carbocyanine dye (DiI-LDL) was used to ... More
An actin cytoskeleton with evolutionarily conserved functions in the absence of canonical actin-binding proteins.
Giardia intestinalis, a human intestinal parasite and member of what is perhaps the earliest-diverging eukaryotic lineage, contains the most divergent eukaryotic actin identified to date and is the first eukaryote known to lack all canonical actin-binding proteins (ABPs). We sought to investigate the properties and functions of the actin cytoskeleton ... More
Beta2 integrins modulate the initiation and progression of atherosclerosis in low-density lipoprotein receptor knockout mice.
AuthorsMerched A, Tollefson K, Chan L,
JournalCardiovasc Res
PubMed ID19843511
Beta2 integrin-mediated adhesion is thought to be a key event in cardiovascular disease. However, results of clinical trials targeting these molecules have been disappointing. Here, we investigated the effect of inactivation of beta2 integrins at different stages of atherosclerosis by timed bone marrow transplantation (BMT) of CD18(-/-) cells in low-density ... More
Cholesterol synthesis and import contribute to protective cholesterol increments in acute myeloid leukemia cells.
AuthorsBanker DE, Mayer SJ, Li HY, Willman CL, Appelbaum FR, Zager RA,
JournalBlood
PubMed ID15161671
Cholesterol levels are abnormally increased in many acute myeloid leukemia (AML) samples exposed in vitro to chemotherapy. Blocking these acute cholesterol responses selectively sensitizes AML cells to therapeutics. Thus, defining the molecular mechanisms by which AML cells accomplish these protective cholesterol increments might elucidate novel therapeutic targets. We now report ... More
Cardiac steroids induce changes in recycling of the plasma membrane in human NT2 cells.
AuthorsRosen H, Glukhman V, Feldmann T, Fridman E, Lichtstein D
JournalMol Biol Cell
PubMed ID14718569
Cardiac steroids (CSs) are specific inhibitors of Na+, K(+)-ATPase activity. Although the presence of CS-like compounds in animal tissues has been established, their physiological role is not evident. In the present study, treatment of human NT2 cells with physiological concentrations (nanomolar) of CSs caused the accumulation of large vesicles adjacent ... More
Endocytosis in skeletal muscle fibers.
AuthorsKaisto T, Rahkila P, Marjomäki V, Parton RG, Metsikkö K
JournalExp Cell Res
PubMed ID10585279
Defining the organization of endocytic pathway in multinucleated skeletal myofibers is crucial to understand the routing of membrane proteins, such as receptors and glucose transporters, through this system. Here we analyzed the organization of the endocytic trafficking pathways in isolated rat myofibers. We found that sarcolemmal-coated pits and transferrin receptors ... More
Dynamic sequestration of the recycling compartment by classical protein kinase C.
AuthorsIdkowiak-Baldys J, Becker KP, Kitatani K, Hannun YA
JournalJ Biol Chem
PubMed ID16751194
It has been previously shown that upon sustained stimulation (30-60 min) with phorbol esters, protein kinase C (PKC) alpha and betaII become sequestered in a juxtanuclear region, the pericentrion. The activation of PKC also results in sequestration of transferrin, suggesting a role for PKC in regulating endocytosis and sequestration of ... More
Trafficking of spontaneously endocytosed MHC proteins.
AuthorsChiu I, Davis DM, Strominger JL
JournalProc Natl Acad Sci U S A
PubMed ID10570178
Class I MHC protein primarily presents endogenous antigen but also may present exogenous antigen. Here, we investigated the intracellular pathway of spontaneously internalized class I MHC protein by confocal microscopy. beta(2)-microglobulin (beta(2)m), labeled with a single fluorophore, was exchanged at the surface of B cell transfectants to specifically mark cell ... More
Regulation of low-density lipoprotein receptor expression in triple negative breast cancer by EGFR-MAPK signaling.
Authors
JournalSci Rep
PubMed ID34504181
Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles.
Authors
JournalACS Nano
PubMed ID28112950
The novel llama-human chimeric antibody has potent effect in lowering LDL-c levels in hPCSK9 transgenic rats.
Authors
JournalClin Transl Med
PubMed ID32056048
Resistin Modulates Low-Density Lipoprotein Cholesterol Uptake in Human Placental Explants via PCSK9.
Authors
JournalReprod Sci
PubMed ID35467263
Stabilin 1 and 2 are important regulators for cellular uptake of apolipoprotein B-containing lipoproteins in zebrafish.
Authors
JournalAtherosclerosis
PubMed ID35247629
PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux.
Authors
JournalJ Neurosci
PubMed ID31061090
NBEAL1 controls SREBP2 processing and cholesterol metabolism and is a susceptibility locus for coronary artery disease.
Authors
JournalSci Rep
PubMed ID32161285
Low-dose metronomic doxorubicin inhibits mobilization and differentiation of endothelial progenitor cells through REDD1-mediated VEGFR-2 downregulation.
Authors
JournalBMB Rep
PubMed ID34488932
Defective Transcytosis of APP and Lipoproteins in Human iPSC-Derived Neurons with Familial Alzheimer's Disease Mutations.
Authors
JournalCell Rep
PubMed ID27732852
Caspase 8 promotes peripheral localization and activation of Rab5.