Low Density Lipoprotein from Human Plasma, BODIPY™ FL complex (BODIPY™ FL LDL) - FAQs

5 个常见问题解答

Can the LDL in BODIPY FL dye-labeled LDL be oxidized?

We do not recommend doing this as the oxidation process (such as oxidation with copper sulphate) may result in the degradation of the BODIPY FL dye.

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Is the LDL in BODIPY FL LDL acetylated?

The LDL in BODIPY FL LDL is not acetylated.

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Can I use methanol or acetone for fixation of cells stained with BODIPY FL LDL?

If the BODIPY FL LDL is applied to live cells first and then fixed, the use of methanol or acetone fixation is not recommended. Exposure to alcohols, acetone, and other organic solvents can dissociate the BODIPY FL dye from the LDL.

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Will BODIPY FL LDL be retained upon permeabilization?

No. Exposure to detergents and surfactants can dissociate the BODIPY FL dye from the LDL.

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How is the BODIPY FL dye bound to the LDL in BODIPY FL LDL?

The BODIPY FL dye is complexed to hydrophobic domains of the LDL; it is not covalently attached.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.