Acute lung injury but not sepsis is associated with increased colony formation by peripheral blood mononuclear cells.
AuthorsBurnham EL, Mealer M, Gaydos J, Majka S, Moss M,
JournalAm J Respir Cell Mol Biol
PubMed ID19843706
Acute lung injury (ALI) and severe sepsis are common critical illnesses associated with the mobilization of bone marrow-derived cells into the circulation. By identifying and determining these cells' functional characteristics, unique prognostic biomarkers can be developed to help investigators understand the mechanisms underlying the pathophysiology of these disorders. We previously ... More
An apolipoprotein E-mimetic stimulates axonal regeneration and remyelination after peripheral nerve injury.
AuthorsLi FQ, Fowler KA, Neil JE, Colton CA, Vitek MP,
JournalJ Pharmacol Exp Ther
PubMed ID20406857
Elevated apolipoprotein E (apoE) synthesis within crushed sciatic nerves advocates that apoE could benefit axonal repair and reconstruction of axonal and myelin membranes. We created an apoE-mimetic peptide, COG112 (acetyl-RQIKIWFQNRRMKWKKCLRVRLASHLRKLRKRLL-amide), and found that postinjury treatment with COG112 significantly improved recovery of motor and sensory function following sciatic nerve crush in ... More
MacMARCKS mutation blocks macrophage phagocytosis of zymosan.
AuthorsZhu Z, Bao Z, Li J
JournalJ Biol Chem
PubMed ID7629059
'A major protein kinase C substrate, MacMARCKS (F52, MPR), was examined for its role in phagocytosis. In macrophage-phagocytosing zymosan particles, MacMARCKS was concentrated around nascent phagosomes as detected by immunofluorescent microscopy. The effector domain of MacMARCKS contains the phosphorylation sites, a calmodulin binding site, as well as a putative actin ... More
Identification and characterization of murine SCARA5, a novel class A scavenger receptor that is expressed by populations of epithelial cells.
AuthorsJiang Y, Oliver P, Davies KE, Platt N
JournalJ Biol Chem
PubMed ID16407294
'Epithelia are positioned at a critical interface to prevent invasion by microorganisms from the environment. Pattern recognition receptors are important components of innate immunity because of their ability to interact with specific microbe-associated structures and initiate immune responses. Several distinct groups of receptors have been recognized. One of these, the ... More
Direct evidence of a connection between autotransplanted microvessel fragments and the host microvascular system.
AuthorsNakano M, Nakajima Y, Tsuchida Y, Kudo S, Nakamura H, Fukuda O
JournalInt J Microcirc Clin Exp
PubMed ID9378565
'There are no reports on the autologous transplantation and patency of microvessels in living tissue. We autotransplanted microvessel fragments (Mvf) labeled with DiI-Ac-LDL into the peritoneum and then observed the peritoneum for 7 days postoperatively with a conventional fluorescence or laser scanning confocal microscope. We illustrated a neomicrovascular network of ... More
Inducible expression of lectin-like oxidized LDL receptor-1 in vascular endothelial cells.
AuthorsKume N, Murase T, Moriwaki H, Aoyama T, Sawamura T, Masaki T, Kita T
JournalCirc Res
PubMed ID9710125
'Endothelial dysfunction, or activation, elicited by oxidized LDL (Ox-LDL) or its lipid constituent, has been implicated in the initiation and progression of atherosclerosis. We have recently identified a C-type lectin-like molecule, designated lectin-like Ox-LDL receptor-1 (LOX-1), which acts as a cell-surface receptor for Ox-LDL in cultured vascular endothelial cells. In ... More
Effect of autotransplantation of microvessel fragments on experimental random-pattern flaps in the rat.
AuthorsNakano M, Nakajima Y, Kudo S, Tsuchida Y, Nakamura H, Fukuda O
JournalEur Surg Res
PubMed ID9627211
'We examined whether autotransplantation of microvessel fragments (Mvf) and/or myofibroblasts (Mf) into an in vivo skin flap model might improve the survival of the ischemic flap. If so, this could improve blood perfusion, increase blood flow, and improve the survival of the flap. A skin flap was raised on the ... More
Tissue-engineered heart valves. Autologous valve leaflet replacement study in a lamb model.
AuthorsShinoka T, Ma PX, Shum-Tim D, Breuer CK, Cusick RA, Zund G, Langer R, Vacanti JP, Mayer JE
JournalCirculation
PubMed ID8901739
'BACKGROUND: We have previously reported the successful creation of tissue-engineered valve leaflets and the implantation of these autologous tissue leaflets in the pulmonary valve position. This study was designed to trace cultured cells that were seeded onto a biodegradable polymer with the use of a 1,1'-dioctadecyl-3,3,3' 3'-tetramethylindo-carbocyanine perchlorate (Di-1) cell-labeling ... More
No evidence of transdifferentiation of human endothelial progenitor cells into cardiomyocytes after coculture with neonatal rat cardiomyocytes.
AuthorsGruh I, Beilner J, Blomer U, Schmiedl A, Schmidt-Richter I, Kruse ML, Haverich A, Martin U
JournalCirculation
PubMed ID16520414
'BACKGROUND: Recent studies have suggested the differentiation of human endothelial progenitor cells (huEPCs) isolated from peripheral blood into cardiomyocytes. This study investigates whether, when cocultured, neonatal rat cardiomyocytes (NRCMs) can induce transdifferentiation of huEPCs into cardiomyocytes. METHODS AND RESULTS: Coculture experiments with 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine (DiI)-labeled huEPCs and NRCMs have been performed. ... More
Acquisition of secretion of transforming growth factor-beta 1 leads to autonomous suppression of scavenger receptor activity in a monocyte-macrophage cell line, THP-1.
AuthorsNishimura N, Harada-Shiba M, Tajima S, Sugano R, Yamamura T, Qiang QZ, Yamamoto A
JournalJ Biol Chem
PubMed ID9430696
'Macrophage cells derived from the human monocytic leukemia cell line, THP-1, accumulate esterified cholesterol when cultivated in the presence of acetylated low density lipoprotein (Ac-LDL) through scavenger receptors (ScR). In the present study, we isolated a subtype of THP-1 cells that failed to accumulate esterified cholesterol when cultivated in the ... More
Selective propagation of retinal pericytes in mixed microvascular cell cultures using L-leucine-methyl ester.
AuthorsLee CS, Patton WF, Chung-Welch N, Chiang ET, Spofford KH, Shepro D
JournalBiotechniques
PubMed ID9762446
'Endothelial cell (EC) propagation has been simplified by developing cell-specific selection criteria. Methods commonly used for selectively isolating EC include: (i) differential sieving of disaggregated tissue, (ii) differential plating of cells on extracellular matrices, (iii) lectin affinity isolation of cell populations and (iv) fluorescence-activated cell sorting of cells labeled with ... More
Class A scavenger receptor up-regulation in smooth muscle cells by oxidized low density lipoprotein. Enhancement by calcium flux and concurrent cyclooxygenase-2 up-regulation.
AuthorsMietus-Snyder M, Gowri MS, Pitas RE
JournalJ Biol Chem
PubMed ID10837497
'Oxidative stress caused by phorbol esters or reactive oxygen up-regulates the class A scavenger receptor (SR-A) in human smooth muscle cells (SMC), which normally do not express this receptor. The increase in SR-A expression correlates with activation of the redox-sensitive transcription factors activating protein-1 c-Jun and CCAAT enhancer-binding protein beta. ... More
Blood vessel formation in the avian limb bud involves angioblastic and angiotrophic growth.
AuthorsBrand-Saberi B, Seifert R, Grim M, Wilting J, Kühlewein M, Christ B
JournalDev Dyn
PubMed ID7734735
'The vasculature of the avian limb bud takes its origin from the intersomitic vessels as can be shown by ink perfusion of the embryo. While the primitive vessels form a central network in the early limb bud, an area of about 100 microns in width from the ectoderm inward remains ... More
Fresh mouse peritoneal macrophages have low scavenger receptor activity.
AuthorsKim JG, Keshava C, Murphy AA, Pitas RE, Parthasarathy S
JournalJ Lipid Res
PubMed ID9392418
'Peritoneal macrophages are easily isolated by lavage, suggesting that they are either nonadherent or weakly adherent in situ. Cultured macrophages express class A scavenger receptors (SCR), which mediate Ca2+-independent adhesion in vitro. We examined fresh peritoneal macrophages from mice and from women with endometriosis to determine whether the adherence of ... More
Characterization of ameboid microglia isolated from developing mammalian brain.
AuthorsGiulian D, Baker TJ
JournalJ Neurosci
PubMed ID3018187
'Ameboid microglia are isolated from the cerebral tissue of neonatal rat by selective cell adhesion to plastic. Histochemical markers show that the microglial preparations are homogeneous (95 +/- 3%) and represent a 10% yield from starting cultures. Isolated ameboid microglia contain nonspecific esterase activity, the macrophage surface antigens MAC-1 and ... More
Determination of LDL- and scavenger-receptor activity in adherent and non-adherent cultured cells with a new single-step fluorometric assay.
AuthorsTeupser D, Thiery J, Walli AK, Seidel D
JournalBiochim Biophys Acta
PubMed ID8908153
'Lipoproteins labeled with 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate (DiI) are widely used to visualize LDL-and scavenger-receptor activity in cultured cells. The purpose of this study was to evaluate a new single-step fluorometric assay with high sensitivity for the quantitative determination of the LDL- or scavenger-receptor activity in adherent and non-adherent cells. We used ... More
Intravascular location of breast cancer cells after spontaneous metastasis to the lung.
AuthorsWong CW, Song C, Grimes MM, Fu W, Dewhirst MW, Muschel RJ, Al-Mehdi AB
JournalAm J Pathol
PubMed ID12213701
'In this study, we examined the hypothesis that early pulmonary metastases form within the vasculature. We introduced primary tumors in immunocompromised mice by subcutaneous injection of murine breast carcinoma cells (4T1) expressing green fluorescent protein. Isolated ventilated and perfused lungs from these mice were examined at various times after tumor ... More
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
Characterization of the scavenger receptor on bovine cerebral endothelial cells in vitro.
Authorsde Vries HE, Kuiper J, de Boer AG, van Berkel TJ, Breimer DD
JournalJ Neurochem
PubMed ID7901334
'Primary cultures of bovine brain capillary endothelial cells (BCEC), possessing tight junctions and high levels of gamma-glutamyl transpeptidase, were used as an in vitro model for the blood-brain barrier. The interaction of acetylated low density lipoprotein (AcLDL) with BCEC was studied to characterize the scavenger receptor on these cells. A ... More
Receptor-mediated transcytosis of transferrin across the blood-brain barrier.
AuthorsFishman JB, Rubin JB, Handrahan JV, Connor JR, Fine RE
JournalJ Neurosci Res
PubMed ID3694713
'The perfusion of rat brain with 125I-transferrin resulted in a receptor-mediated uptake of transferrin into the endothelium of the blood-brain barrier followed by its detection in the brain. During a pulse-chase experiment, 125I-transferrin accumulated in the endothelial cells during the pulse, with a decrease of this intraendothelial radioactivity during the ... More
The TNF-like protein 1A-death receptor 3 pathway promotes macrophage foam cell formation in vitro.
AuthorsMcLaren JE, Calder CJ, McSharry BP, Sexton K, Salter RC, Singh NN, Wilkinson GW, Wang EC, Ramji DP,
JournalJ Immunol
PubMed ID20410491
'TNF-like protein 1A (TL1A), a TNF superfamily cytokine that binds to death receptor 3 (DR3), is highly expressed in macrophage foam cell-rich regions of atherosclerotic plaques, although its role in foam cell formation has yet to be elucidated. We investigated whether TL1A can directly stimulate macrophage foam cell formation in ... More
Role of lysine residues of plasma lipoproteins in high affinity binding to cell surface receptors on human fibroblasts.
AuthorsWeisgraber KH, Innerarity TL, Mahley RW
JournalJ Biol Chem
PubMed ID214439
'The low density lipoprotein (LDL) cell surface receptors on human fibroblasts grown in culture bind specific plasma lipoproteins, initiating a series of events which regulate intracellular cholesterol metabolism. Specificity for the interaction with the receptors resides with the protein moieties of the lipoproteins, specifically with the B and E apoproteins ... More
The HHQK domain of beta-amyloid provides a structural basis for the immunopathology of Alzheimer's disease.
AuthorsGiulian D, Haverkamp LJ, Yu J, Karshin W, Tom D, Li J, Kazanskaia A, Kirkpatrick J, Roher AE
JournalJ Biol Chem
PubMed ID9792685
'The beta-amyloid peptide 1-42 (Abeta1-42), a major component of neuritic and core plaques found in Alzheimer''s disease, activates microglia to kill neurons. Selective modifications of amino acids near the N terminus of Abeta showed that residues 13-16, the HHQK domain, bind to microglial cells. This same cluster of basic amino ... More
In situ labelling of vascular endothelium with fluorescent acetylated low density lipoprotein.
AuthorsNetland PA, Zetter BR, Via DP, Voyta JC
JournalHistochem J
PubMed ID3833857
'Acetylated low density lipoprotein is metabolized by a receptor-mediated process in endothelial cells. We have used the lipoprotein labelled with the fluorescent probe 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate to localize endothelial cells lining blood vessels. Following intravenous injection of the labelled lipoprotein, the vascular sinusoids and all other hepatic blood vessels were clearly ... More
Isolation and culture of bovine endothelial cells of endoneurial origin.
AuthorsKanda T, Iwasaki T, Yamawaki M, Ikeda K
JournalJ Neurosci Res
PubMed ID9335264
'Penetration of immunoglobulins and/or migration of activated lymphocytes into peripheral nervous system (PNS) parenchyma are the initial key steps to develop immunological disorders of PNS including Guillain-Barré syndrome, IgM neuropathy and chronic inflammatory demyelinating polyradiculoneuropathy. Hence, it is important to know the cellular property of endothelial cells of endoneurial tissue ... More
Sequestration of acetylated LDL and cholesterol crystals by human monocyte-derived macrophages.
AuthorsKruth HS, Skarlatos SI, Lilly K, Chang J, Ifrim I
JournalJ Cell Biol
PubMed ID7698980
'Monocyte-derived macrophages accumulate and process cholesterol in atherosclerotic lesions. Because of the importance of this process, we examined the interaction of cholesterol crystals and acetylated low density lipoprotein (AcLDL) with human monocyte-macrophages in a combined chemical and morphological study. These two forms of cholesterol induced extensive compartmentalization of the macrophage ... More
Human omental microvascular endothelial and mesothelial cells: characterization of two distinct mesodermally derived epithelial cells.
AuthorsChung-Welch N, Patton WF, Shepro D, Cambria RP
JournalMicrovasc Res
PubMed ID9327382
'Human omental microvascular endothelial (HOME) and mesothelial (MESO) cells share many phenotypic properties, but can be characterized from one another based upon a comprehensive panel of endothelial and mesothelial markers. Traditional cell markers such as von-Willebrand factor, DiI-Ac-LDL, and Ulex europaeus I lectin are not sufficient to distinguish between HOME ... More
Macrophages adhere to glucose-modified basement membrane collagen IV via their scavenger receptors.
Authorsel Khoury J, Thomas CA, Loike JD, Hickman SE, Cao L, Silverstein SC
JournalJ Biol Chem
PubMed ID8144597
'Scavenger receptors have been reported to mediate macrophage adhesion to serum-coated plastic surfaces. We report here that scavenger receptors promote the divalent cation independent adhesion of human monocytes and macrophages to surfaces coated with non-enzymatically glycated collagen IV but not to surfaces coated with native collagen IV. Ligands for scavenger ... More
Characterization of Cobblestone mitral valve interstitial cells.
AuthorsZacks S, Rosenthal A, Granton B, Havenith M, Opas M, Gotlieb AI
JournalArch Pathol Lab Med
PubMed ID1863188
'Mitral valve interstitial cells (MVICs) are important in the structure and function of the valve. In a cell culture system that used explants to harvest MVICs, we found that after several passages, some of the cultures at confluence formed a monolayer of cobblestone-shaped cells (CB type). The rest of the ... More
A phage display screen and binding studies with acetylated low density lipoprotein provide evidence for the importance of the scavenger receptor cysteine-rich (SRCR) domain in the ligand-binding function of MARCO.
AuthorsChen Y, Sankala M, Ojala JR, Sun Y, Tuuttila A, Isenman DE, Tryggvason K, Pikkarainen T
JournalJ Biol Chem
PubMed ID16524885
'MARCO is a class A scavenger receptor capable of binding both gram-negative and -positive bacteria. Using the surface plasmon resonance technique, we show here that a recombinant, soluble form of MARCO, sMARCO, binds the major gram-negative and -positive bacterial surface components, lipopolysaccharide and lipoteichoic acid. Yet, the interaction of these ... More
Prostaglandin E2 and 4-aminopyridine prevent the lipopolysaccharide-induced outwardly rectifying potassium current and interleukin-1beta production in cultured rat microglia.
AuthorsCaggiano AO, Kraig RP
JournalJ Neurochem
PubMed ID9603200
'Brain inflammation includes microglial activation and enhanced production of diffusible chemical mediators, including prostaglandin E2. Prostaglandin E2 is generally considered a proinflammatory molecule, but it also promotes neuronal survival and down-regulates some aspects of microglial activation. It remains unknown, however, if and how prostaglandin E2 prevents microglial activation. In primary ... More
Human Thy-1 is cytokine-inducible on vascular endothelial cells and is a signaling molecule regulated by protein kinase C.
AuthorsMason JC, Yarwood H, Tárnok A, Sugars K, Harrison AA, Robinson PJ, Haskard DO
JournalJ Immunol
PubMed ID8752941
'Thy-1 is a glycosylphosphatidylinositol-anchored member of the Ig superfamily whose function, particularly in the human, remains unknown. We have demonstrated that human Thy-1 is expressed on endothelial cells (EC) both in situ and on the surface of cultured human umbilical vein EC and dermal microvascular EC (DMEC). The expression of ... More
Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice.
AuthorsEwing P, Wilke A, Brockhoff G, Andreesen R, Eissner G, Holler E, Gerbitz A
JournalJ Immunol Methods
PubMed ID14659921
'The isolation of primary endothelial cells from murine tissues has long been a challenge and remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of ... More
PDGF, TGF-beta, and heterotypic cell-cell interactions mediate endothelial cell-induced recruitment of 10T1/2 cells and their differentiation to a smooth muscle fate.
AuthorsHirschi KK, Rohovsky SA, D'Amore PA
JournalJ Cell Biol
PubMed ID9566978
'We aimed to determine if and how endothelial cells (EC) recruit precursors of smooth muscle cells and pericytes and induce their differentiation during vessel formation. Multipotent embryonic 10T1/2 cells were used as presumptive mural cell precursors. In an under-agarose coculture, EC induced migration of 10T1/2 cells via platelet-derived growth factor ... More
Thrombospondin causes activation of latent transforming growth factor-beta secreted by endothelial cells by a novel mechanism.
AuthorsSchultz-Cherry S, Murphy-Ullrich JE
JournalJ Cell Biol
PubMed ID8349738
'Thrombospondin (TSP) forms specific complexes with transforming growth factor-beta (TGF-beta) in the alpha granule releasate of platelets and these TSP-TGF-beta complexes inhibit the growth of bovine aortic endothelial cells (BAE). In these studies, we report that TSP stripped of associated TGF-beta (sTSP) retained growth inhibitory activity which was partially reversed ... More
Visualization of the interaction of native and modified lipoproteins with parenchymal, endothelial and Kupffer cells from human liver.
AuthorsKleinherenbrink-Stins MF, van de Boom JH, Schouten D, Roholl PJ, Niels van der Heyde M, Brouwer A, van Berkel TJ, Knook DL
JournalHepatology
PubMed ID1648542
'The interaction of low density lipoprotein, acetylated low density lipoprotein and apolipoprotein E-free high density lipoprotein with parenchymal, endothelial and Kupffer cells of human liver was visualized. For this purpose, the fluorescent phospholipid analog 1,1'-dioctadecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate was used to label the lipoproteins. The involvement of both parenchymal and nonparenchymal ... More
Regulation of transforming growth factor-beta activation by discrete sequences of thrombospondin 1.
AuthorsSchultz-Cherry S, Chen H, Mosher DF, Misenheimer TM, Krutzsch HC, Roberts DD, Murphy-Ullrich JE
JournalJ Biol Chem
PubMed ID7706271
'Transforming growth factor-beta (TGF-beta) is a potent growth regulatory protein secreted by virtually all cells in a latent form. A major mechanism of regulating TGF-beta activity occurs through factors that control the processing of the latent to the biologically active form of the molecule. We have shown previously that thrombospondin ... More
Non-neuronal enolase is an endothelial hypoxic stress protein.
AuthorsAaronson RM, Graven KK, Tucci M, McDonald RJ, Farber HW
JournalJ Biol Chem
PubMed ID7499243
'The hypoxia-associated proteins (HAPs) are five cell-associated stress proteins (M(r) 34, 36, 39, 47, and 57) up-regulated in cultured vascular endothelial cells (EC) exposed to hypoxia. While hypoxic exposure of other cell types induces heat shock and glucose-regulated proteins, EC preferentially up-regulate HAPs. In order to identify the 47-kDa HAP, ... More
Applications of ratio fluorescence microscopy in the study of cell physiology.
AuthorsDunn KW, Mayor S, Myers JN, Maxfield FR
JournalFASEB J
PubMed ID8005385
'Quantitative fluorescence microscopy is becoming an increasingly important tool in the study of cell biology. Fluorescence microscopy has long been used for qualitative characterizations of subcellular distributions of proteins, lipids, nucleic acids, and ions, but quantifying these distributions is complicated by a variety of optical, biological, and physical factors. Many ... More
Fluorescence studies of macrophage recognition and endocytosis of native and acetylated low-density lipoprotein.
AuthorsBerg KA, Berry ML, Sapareto SA, Petty HR
JournalBiochim Biophys Acta
PubMed ID3730431
'Macrophage recognition and endocytosis of 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate (diI)-labeled low-density lipoprotein (LDL) and acetyl LDL (Ac-LDL) was studied using fluorescence flow cytometry and fluorescence video intensification microscopy. RAW264 macrophages and U937 monocytes were grown in the tissue culture media in the presence and absence of LDL and Ac-LDL. Several lines of ... More
Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.
AuthorsHan X, Kitamoto S, Wang H, Boisvert WA,
JournalFASEB J
PubMed ID20354139
'In atherogenesis, macrophage foam cell formation is modulated by pathways involving both the uptake and efflux of cholesterol. We recently showed that interleukin-10 (IL-10) modulates lipid metabolism by enhancing both uptake and efflux of cholesterol in macrophages. However, the mechanistic details of these properties in vivo have been unclear. Thus, ... More
Rotating wall vessel as a new in vitro shear stress generation system: application to rat coronary endothelial cell cultures.
AuthorsMorin JP, Preterre D, Keravec V, Thuillez C
JournalCell Biol Toxicol
PubMed ID14686615
'In this paper, we describe a simple new design for the application of controlled, top-hat profiled wall shear stress forces in a way that is independent of hydrostatic pressure and oxygen tension, based on a rotating wall vessel system. This system has been applied to the culture of rat coronary ... More
Expression of the acetyl low density lipoprotein receptor by rabbit fibroblasts and smooth muscle cells. Up-regulation by phorbol esters.
AuthorsPitas RE
JournalJ Biol Chem
PubMed ID2373709
'The acetyl low density lipoprotein (LDL), or scavenger, receptor, which binds modified forms of LDL, was thought to be expressed only on macrophages and endothelial cells. We demonstrate that rabbit fibroblasts and smooth muscle cells bind, internalize, and degrade acetoacetylated LDL, a ligand for the acetyl LDL receptor. Degradation is ... More
Expression of modified low-density lipoprotein receptors by trabecular meshwork cells.
AuthorsChang IL, Elner G, Yue YJ, Cornicelli A, Kawa JE, Elner VM
JournalCurr Eye Res
PubMed ID1802612
'We examined the incorporation of fluoresceinated low-density lipoprotein (LDL) and acetylated or acetoacetylated low-density lipoprotein (A-LDL or AA-LDL) by a number of ocular cells in culture. All the cells investigated, including bovine, monkey, human trabecular meshwork cells, human corneal endothelial cells, human corneal stromal cells and human scleral cells, took ... More
Essential control of an endothelial cell ISOC by the spectrin membrane skeleton.
AuthorsWu S, Sangerman J, Li M, Brough GH, Goodman SR, Stevens T
JournalJ Cell Biol
PubMed ID11564759
'Mechanism(s) underlying activation of store-operated Ca2+ entry currents, ISOC, remain incompletely understood. F-actin configuration is an important determinant of channel function, although the nature of interaction between the cytoskeleton and ISOC channels is unknown. We examined whether the spectrin membrane skeleton couples Ca2+ store depletion to Ca2+ entry. Thapsigargin activated ... More
Discovery of antagonists for human scavenger receptor CD36 via an ELISA-like high-throughput screening assay.
AuthorsWang L, Bao Y, Yang Y, Wu Y, Chen X, Si S, Hong B,
JournalJ Biomol Screen
PubMed ID20150587
'CD36, a member of the class B scavenger receptor, is a high-affinity receptor for oxidatively modified low-density lipoprotein (oxLDL). Extensive evidence points to a significant role of CD36 in atherosclerosis and suggests that CD36 could be a potential target for treatment of atherosclerosis. Here, the extracellular domain of human CD36 ... More
Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.
AuthorsVoyta JC, Via DP, Butterfield CE, Zetter BR
JournalJ Cell Biol
PubMed ID6501412
'Acetylated-low density lipoprotein (Ac-LDL) is taken up by macrophages and endothelial cells via the "scavenger cell pathway" of LDL metabolism. In this report, aortic and microvascular endothelial cells internalized and degraded 7-15 times more [125I]-Ac-LDL than did smooth muscle cells or pericytes. Bound [125I]-Ac-LDL was displaced by unlabeled Ac-LDL, but ... More
Ceramide signaling in fenretinide-induced endothelial cell apoptosis.
AuthorsErdreich-Epstein A, Tran LB, Bowman NN, Wang H, Cabot MC, Durden DL, Vlckova J, Reynolds CP, Stins MF, Groshen S, Millard M
JournalJ Biol Chem
PubMed ID12388538
'Stress stimuli can mediate apoptosis by generation of the lipid second messenger, ceramide. Herein we investigate the molecular mechanism of ceramide signaling in endothelial apoptosis induced by fenretinide (N-(4-hydroxyphenyl)retinamide (4-HPR)). 4-HPR, a synthetic derivative of retinoic acid that induces ceramide in tumor cell lines, has been shown to have antiangiogenic ... More
Cyclophilin A is an inflammatory mediator that promotes atherosclerosis in apolipoprotein E-deficient mice.
AuthorsNigro P, Satoh K, O'Dell MR, Soe NN, Cui Z, Mohan A, Abe J, Alexis JD, Sparks JD, Berk BC,
JournalJ Exp Med
PubMed ID21173104
'Cyclophilin A (CyPA; encoded by Ppia) is a ubiquitously expressed protein secreted in response to inflammatory stimuli. CyPA stimulates vascular smooth muscle cell migration and proliferation, endothelial cell adhesion molecule expression, and inflammatory cell chemotaxis. Given these activities, we hypothesized that CyPA would promote atherosclerosis. Apolipoprotein E-deficient (Apoe(-/-)) mice fed ... More
Induction of nitric oxide synthase in glial cells.
AuthorsSimmons ML, Murphy S
JournalJ Neurochem
PubMed ID1379633
'Primary astrocyte cultures, C6 glioma cells, and N18 neuroblastoma cells were assayed for nitric oxide synthase (NOS) activity with a bioassay of cyclic GMP production in RFL-6 fibroblasts. Treatment of astrocyte cultures for 16-18 h with lipopolysaccharide (LPS) induced NOS-like activity that was L-arginine and NADPH dependent, Ca2+ independent, and ... More
Lipoprotein uptake in primary cell cultures of rabbit atherosclerotic lesions. A fluorescence microscopic and flow cytometric study.
AuthorsJaakkola O, Kallioniemi OP, Nikkari T
JournalAtherosclerosis
PubMed ID3348844
'To characterize the lipoprotein metabolism of lipid-filled cells of atherosclerotic lesions, uptake of 3,3''-dioctadecylindocarbocyanine (DiI)-labelled low density lipoprotein (LDL), acetylated LDL (Ac-LDL) and beta-very low density lipoprotein (beta-VLDL) was studied by fluorescence microscopy and flow cytometry in primary cultures of enzymatically dispersed aortic cells from cholesterol-fed rabbits. Most of the ... More
Neovascularization of ischemic myocardium by human bone-marrow-derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function.
AuthorsKocher AA, Schuster MD, Szabolcs MJ, Takuma S, Burkhoff D, Wang J, Homma S, Edwards NM, Itescu S
JournalNat Med
PubMed ID11283669
'Left ventricular remodeling is a major cause of progressive heart failure and death after myocardial infarction. Although neoangiogenesis within the infarcted tissue is an integral component of the remodeling process, the capillary network is unable to support the greater demands of the hypertrophied myocardium, resulting in progressive loss of viable ... More
Induction of macrophage antitumor activity by acetylated low density lipoprotein containing lipophilic muramyl tripeptide.
AuthorsShaw JM, Futch WS, Schook LB
JournalProc Natl Acad Sci U S A
PubMed ID3413079
'A method has been developed for the selective delivery of lipophilic immunomodulators to macrophages, which results in the induction of antitumor activity. This method utilizes exhaustively acetylated low density lipoprotein (acetyl-LDL) to deliver the lipophilic immunomodulator, muramyl tripeptide phosphatidylethanolamine (MTP-PtdEtn; amide composed of N-acetylmuramyl-L-alanyl-D-isoglutamyl-L-alanine and dipalmitoyl phosphatidylethanolamine) to macrophages (M ... More
Brain glia release factors with opposing actions upon neuronal survival.
AuthorsGiulian D, Vaca K, Corpuz M
JournalJ Neurosci
PubMed ID8423475
'Microglia and astroglia have been thought to govern the survival of neurons after damage to the CNS. To investigate these putative glia-neuron relationships, we examined microglia and astroglia secretion products for effects upon growth of cultured neurons. Activated microglia secrete small neurotoxic factors (< 500 Da), while astroglia constitutively release ... More
Removal of venous endothelium with air.
AuthorsBjorling DE, Saban R, Tengowski MW, Gruel SM, Rao VK
JournalJ Pharmacol Toxicol Methods
PubMed ID1489986
'Much research on the activity and half-life of endothelium-derived substances has entailed the removal of endothelium from arteries by mechanical or enzymatic processes. It has been observed that the technique used for the removal of arterial endothelium may profoundly affect smooth muscle function and release of prostanoids by the vessel ... More
Rat microglia exhibit increased density on Alzheimer's plaques in vitro.
AuthorsJoshi SN, Crutcher KA
JournalExp Neurol
PubMed ID9454613
'The relationship of microglia to senile plaques was investigated by culturing glial cells derived from neonatal rat brain on cryostat sections of Alzheimer''s disease (AD) or control brain. Rat microglia were identified by their uptake of DiI-acetylated LDL. Plaques were colocalized using Thioflavin-S staining. Although the number of microglia attached ... More
Microglia in the pineal gland of the neonatal rat: characterization and effects on pinealocyte neurite length and serotonin content.
AuthorsTsai SY, McNulty JA
JournalGlia
PubMed ID9215733
'Microglia in the pineal gland of 1-day-old Sprague-Dawley rats were examined by OX-42 immunocytochemistry and DiI-acetylated-LDL uptake in pineal cell suspension and were found to comprise 3-5% of the total cells in the pineal gland of the neonates. In order to investigate the effects of microglia on pinealocyte structure and ... More
Inflammasome activation of IL-18 results in endothelial progenitor cell dysfunction in systemic lupus erythematosus.
'Systemic lupus erythematosus (SLE) is an autoimmune disease with heterogeneous manifestations including severe organ damage and vascular dysfunction leading to premature atherosclerosis. IFN-a has been proposed to have an important role in the development of lupus and lupus-related cardiovascular disease, partly by repression of IL-1 pathways leading to impairments in ... More
Generation of superoxide anion by brain endothelial cell xanthine oxidase.
AuthorsTerada LS, Willingham IR, Rosandich ME, Leff JA, Kindt GW, Repine JE
JournalJ Cell Physiol
PubMed ID1652587
Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased (greater than 20-fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (O2-) into the extracellular medium. Treatment of EC with tungsten decreased (P less ... More
Requirement for croquemort in phagocytosis of apoptotic cells in Drosophila.
AuthorsFranc NC, Heitzler P, Ezekowitz RA, White K
JournalScience
PubMed ID10373118
Macrophages in the Drosophila embryo are responsible for the phagocytosis of apoptotic cells and are competent to engulf bacteria. Croquemort (CRQ) is a CD36-related receptor expressed exclusively on these macrophages. Genetic evidence showed that crq was essential for efficient phagocytosis of apoptotic corpses but was not required for the engulfment ... More
Type I macrophage scavenger receptor contains alpha-helical and collagen-like coiled coils.
AuthorsKodama T, Freeman M, Rohrer L, Zabrecky J, Matsudaira P, Krieger M
JournalNature
PubMed ID2300204
The macrophage scavenger receptor is a trimeric membrane glycoprotein with unusual ligand-binding properties which has been implicated in the development of atherosclerosis. The trimeric structure of the bovine type I scavenger receptor, deduced by complementary DNA cloning, contains three extracellular C-terminal cysteine-rich domains connected to the transmembrane domain by a ... More
Engagement of the scavenger receptor is not responsible for beta-amyloid stimulation of monocytes to a neurocytopathic state.
AuthorsAntic A, Dzenko KA, Pachter JS
JournalExp Neurol
PubMed ID10683276
Experiments were performed to determine if scavenger receptors (SRs) play a role in amyloid beta (Abeta) stimulation of peripheral blood monocyte (PBM) neurotoxicity. Results indicate that Abeta does not block binding of the SR ligand DiI-acetylated low density lipoprotein to PBM, nor does another SR ligand, fucoidin, inhibit Abeta-PBM binding. ... More
Assessing identity, phenotype, and fate of endothelial progenitor cells.
AuthorsHirschi KK, Ingram DA, Yoder MC,
JournalArterioscler Thromb Vasc Biol
PubMed ID18669889
From the paradigm shifting observations of Harvey, Malpighi, and van Leeuwenhoek, blood vessels have become recognized as distinct and dynamic tissue entities that merge with the heart to form a closed circulatory system. Vessel structures are comprised predominantly of a luminal layer of endothelial cells that is surrounded by some ... More
Brain peptides and glial growth. II. Identification of cells that secrete glia-promoting factors.
AuthorsGiulian D, Young DG
JournalJ Cell Biol
PubMed ID3949881
Glia-promoting factors (GPFs) are brain peptides which stimulate growth of specific macroglial populations in vitro. To identify the cellular sources of GPFs, we examined enriched brain cell cultures and cell lines derived from the nervous system for the production of growth factors. Ameboid microglia secreted astroglia-stimulating peptides, while growing neurons ... More
Somatic frameshift mutations in the BAX gene in colon cancers of the microsatellite mutator phenotype.
AuthorsRampino N, Yamamoto H, Ionov Y, Li Y, Sawai H, Reed JC, Perucho M
JournalScience
PubMed ID9020077
Cancers of the microsatellite mutator phenotype (MMP) show exaggerated genomic instability at simple repeat sequences. More than 50 percent (21 out of 41) of human MMP+ colon adenocarcinomas examined were found to have frameshift mutations in a tract of eight deoxyguanosines [(G)8] within BAX, a gene that promotes apoptosis. These ... More
Site-specific gene expression in vivo by direct gene transfer into the arterial wall.
AuthorsNabel EG, Plautz G, Nabel GJ
JournalScience
PubMed ID2119055
A recombinant beta-galactosidase gene has been expressed in a specific arterial segment in vivo by direct infection with a murine amphotropic retroviral vector or by DNA transfection with the use of liposomes. Several cell types in the vessel wall were transduced, including endothelial and vascular smooth muscle cells. After retroviral ... More
Charged collagen structure mediates the recognition of negatively charged macromolecules by macrophage scavenger receptors.
AuthorsDoi T, Higashino K, Kurihara Y, Wada Y, Miyazaki T, Nakamura H, Uesugi S, Imanishi T, Kawabe Y, Itakura H
JournalJ Biol Chem
PubMed ID8380589
Macrophage scavenger receptors mediate the recognition of a wide range of negatively charged macromolecules including modified low density lipoproteins (LDL). Truncated bovine receptors lacking residues 330-342, which include the conserved lysine cluster of a collagen-like domain, were unable to degrade modified LDL in spite of their expression on the cell ... More
Angiogenesis potential of human limbal stromal niche cells.
AuthorsLi GG, Chen SY, Xie HT, Zhu YT, Tseng SC,
JournalInvest Ophthalmol Vis Sci
PubMed ID22538425
The perivascular localization of stem cell (SC) niches suggests the presence of a vascular niche. We aimed to determine the angiogenesis potential of limbal niche cells (NCs). Human limbal NCs were isolated and serially passaged on plastic or coated Matrigel in embryonic SC medium containing BFGF and leukemia inhibitory factor ... More
VEGF induces differentiation of functional endothelium from human embryonic stem cells: implications for tissue engineering.
AuthorsNourse MB, Halpin DE, Scatena M, Mortisen DJ, Tulloch NL, Hauch KD, Torok-Storb B, Ratner BD, Pabon L, Murry CE,
JournalArterioscler Thromb Vasc Biol
PubMed ID19875721
Human embryonic stem cells (hESCs) offer a sustainable source of endothelial cells for therapeutic vascularization and tissue engineering, but current techniques for generating these cells remain inefficient. We endeavored to induce and isolate functional endothelial cells from differentiating hESCs. To enhance endothelial cell differentiation above a baseline of approximately 2% ... More
FTY720 stimulates 27-hydroxycholesterol production and confers atheroprotective effects in human primary macrophages.
AuthorsBlom T, Bäck N, Mutka AL, Bittman R, Li Z, de Lera A, Kovanen PT, Diczfalusy U, Ikonen E,
JournalCirc Res
PubMed ID20056921
The synthetic sphingosine analog FTY720 is undergoing clinical trials as an immunomodulatory compound, acting primarily via sphingosine 1-phosphate receptor activation. Sphingolipid and cholesterol homeostasis are closely connected but whether FTY720 affects atherogenesis in humans is not known. We examined the effects of FTY720 on the processing of scavenged lipoprotein cholesterol ... More
Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.
AuthorsSever-Chroneos Z, Krupa A, Davis J, Hasan M, Yang CH, Szeliga J, Herrmann M, Hussain M, Geisbrecht BV, Kobzik L, Chroneos ZC,
JournalJ Biol Chem
PubMed ID21123169
Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates ... More
Microengineered liver tissues for drug testing.
AuthorsKhetani SR, Berger DR, Ballinger KR, Davidson MD, Lin C, Ware BR,
Journal
PubMed ID25617027
Drug-induced liver injury (DILI) is a leading cause of drug attrition. Significant and well-documented differences between animals and humans in liver pathways now necessitate the use of human-relevant in vitro liver models for testing new chemical entities during preclinical drug development. Consequently, several human liver models with various levels of ... More
Microglial cells in astroglial cultures: a cautionary note.
AuthorsSaura J,
JournalJ Neuroinflammation
PubMed ID17937799
Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in cultures in which the astrocyte is the predominant ... More
Enhanced mitochondrial superoxide in hyperglycemic endothelial cells: direct measurements and formation of hydrogen peroxide and peroxynitrite.
AuthorsQuijano C, Castro L, Peluffo G, Valez V, Radi R,
JournalAm J Physiol Heart Circ Physiol
PubMed ID17906108
Hyperglycemic challenge to bovine aortic endothelial cells (BAECs) increases oxidant formation and cell damage that are abolished by MnSOD overexpression, implying mitochondrial superoxide (O(2)(.-)) as a central mediator. However, mitochondrial O(2)(.-) and its steady-state concentrations have not been measured directly yet. Therefore, we aimed to detect and quantify O(2)(.-) through ... More
Probucol does not alter acetylated low density lipoprotein uptake by murine peritoneal macrophages.
AuthorsKu G, Schroeder K, Schmidt LF, Jackson RL, Doherty NS
JournalAtherosclerosis
PubMed ID2310425
It has been suggested that the anti-atherogenic effect of probucol (MDL 11,309) in familial hypercholesterolemic rabbits may be due in part to the inhibition of the uptake of modified low density lipoproteins by macrophages in the arterial wall. To test this hypothesis, mice were treated with dietary probucol (0.25%) for ... More
Selective chemical treatment of cellular microdomains using multiple laminar streams.
AuthorsTakayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM
JournalChem Biol
PubMed ID12618184
There are many experiments in which it would be useful to treat a part of the surface or interior of a cell with a biochemical reagent. It is difficult, however, to achieve subcellular specificity, because small molecules diffuse distances equal to the extent of the cell in seconds. This paper ... More
Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle.
AuthorsMatsuura K, Wada H, Nagai T, Iijima Y, Minamino T, Sano M, Akazawa H, Molkentin JD, Kasanuki H, Komuro I
JournalJ Cell Biol
PubMed ID15492039
The concept of the plasticity or transdifferentiation of adult stem cells has been challenged by the phenomenon of cell fusion. In this work, we examined whether neonatal cardiomyocytes fuse with various somatic cells including endothelial cells, cardiac fibroblasts, bone marrow cells, and endothelial progenitor cells spontaneously in vitro. When cardiomyocytes ... More
Increased uptake of monocyte-treated low density lipoproteins by aortic endothelium in vivo.
AuthorsGörög P, Kakkar VV
JournalAtherosclerosis
PubMed ID3038135
A new technique was elaborated for measuring LDL uptake by rat aortic endothelial cells in vivo, using a fluorescent marker (Dil)-labelled LDL and quantifying the fluorescence in cells selectively removed from the aorta. This technique was used to study the endothelial uptake of LDL modified by activated human monocytes (LDL-A) ... More
Detection of the low-density-lipoprotein receptor with biotin-low-density lipoprotein. A rapid new method for ligand blotting.
AuthorsWade DP, Knight BL, Soutar AK
JournalBiochem J
PubMed ID4052026
A new technique has been developed to identify low-density-lipoprotein (LDL) receptors on nitrocellulose membranes, after transfer from SDS/polyacrylamide gels, by ligand blotting with biotin-modified LDL. Modification with biotin hydrazide of periodate-oxidized lipoprotein sugar residues does not affect the ability of the lipoprotein to bind to the LDL receptor. Bound lipoprotein ... More
Potential role for scavenger receptors of human monocytes in the killing of Schistosoma mansoni.
AuthorsXu X, Remold HG, Caulfield JP
JournalAm J Pathol
PubMed ID8456931
Human low-density lipoproteins (LDL) bind specifically and saturably to the surface of the trematode parasite, Schistosoma mansoni, in vitro. Here we have tested whether human monocytes process the bound LDL. Monocytes obtained by leukapheresis generate H2O2, kill schistosomula, and were seen here endocytosing fluorescently labeled human LDL that was bound ... More
Fluorescent labeling of endothelial cells allows in vivo, continuous characterization of the vascular development of Xenopus laevis.
AuthorsLevine AJ, Munoz-Sanjuan I, Bell E, North AJ, Brivanlou AH
JournalDev Biol
PubMed ID12606281
Appropriate blood supply and vascular development are necessary in development and in cancer, heart disease, and diabetes. Here, we report the use of DiI-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) to label endothelial cells and characterize the vasculature of live Xenopus embryos. The atlas we have created provides a detailed map of ... More
Cholesterol-induced macrophage apoptosis requires ER stress pathways and engagement of the type A scavenger receptor.
AuthorsDevries-Seimon T, Li Y, Yao PM, Stone E, Wang Y, Davis RJ, Flavell R, Tabas I
JournalJ Cell Biol
PubMed ID16203857
Macrophage death in advanced atherosclerosis promotes necrosis and plaque destabilization. A likely cause of macrophage death is accumulation of free cholesterol (FC) in the ER, leading to activation of the unfolded protein response (UPR) and C/EBP homologous protein (CHOP)-induced apoptosis. Here we show that p38 MAPK signaling is necessary for ... More
The effects of a shear flow on the uptake of LDL and acetylated LDL by an EC monoculture and an EC-SMC coculture.
AuthorsNiwa K, Kado T, Sakai J, Karino T
JournalAnn Biomed Eng
PubMed ID15117027
To elucidate the mechanisms of localized genesis and development of atherosclerosis and anastomotic intimal hyperplasia in man, a coculture of bovine aortic endothelial cells (ECs) and smooth muscle cells (SMCs) was prepared, and the effects of a shear flow on the uptake of lipoproteins by the cells was studied by ... More
Oscillatory pericellular proteolysis and oxidant deposition during neutrophil locomotion.
To better understand the mechanism of leukocyte migration in complex environments, model extracellular matrices were prepared using gelatin, Hanks' solution, Bodipy-BSA (fluorescent upon proteolysis), and dihydrotetramethylrosamine or hydroethidine (fluorescent upon oxidation). Using quantitative microfluorometry, neutrophil-mediated extracellular pulses of reactive oxygen metabolites (ROMs) and pericellular proteolysis were periodically observed showing that ... More
Effect of shear stress on cytosolic Ca2+ of calf pulmonary artery endothelial cells.
AuthorsSchilling WP, Mo M, Eskin SG
JournalExp Cell Res
PubMed ID1370060
The purpose of the present study was to determine if hemodynamic shear stress increases free cytosolic Ca2+ concentration ([Ca2+]i) of cultured pulmonary artery endothelial cells exposed to steady laminar fluid flow in a parallel plate chamber. Average [Ca2+]i was estimated by measuring cell-associated fura-2 fluorescence using microfluorimetric analysis. To determine ... More
In vitro studies of human choroidal endothelial cells.
Vascular endothelial cells play an important role in progression or healing of various retinal or choroidal diseases and they have a broad organ specificity. We have grown and studied human choroidal endothelial cells (CECs) cultured in a collagen gel, where they form tube-like structures. CECs were differentially isolated from choroidal ... More
Establishment of a human hemangiosarcoma cell line (ISO-HAS).
AuthorsMasuzawa M, Fujimura T, Hamada Y, Fujita Y, Hara H, Nishiyama S, Katsuoka K, Tamauchi H, Sakurai Y
JournalInt J Cancer
PubMed ID10188735
A cell line (ISO-HAS) has been established from tumor tissue of a human hemangiosarcoma arising on the scalp by the use of conditioned medium from a murine-phenotypic angiosarcoma cell line (ISOS-1). Cells have been cultured for more than 2 years with up to 100 passages. The cells retained endothelial-cell properties, ... More
Characterization of human monocytic cell line, U937, in taking up acetylated low-density lipoprotein and cholesteryl ester accumulation. A flow cytometric and HPLC study.
AuthorsSuzuki K, Sakata N, Kitani A, Hara M, Hirose T, Hirose W, Norioka K, Harigai M, Kawagoe M, Nakamura H
JournalBiochim Biophys Acta
PubMed ID2302423
The uptake of LDL and acetylated LDL and the ability of cholesteryl ester accumulation by cells of a human monocytic cell line, U937, has been characterized by flow cytometric assay using a fluorescent probe, DiI, and by high-performance liquid chromatography (HPLC). The increase of mean fluorescence intensity of U937 incubated ... More
Living isolated cells from inner ear vessels: a new approach for studying the regulation of cochlear microcirculation and vascular permeability.
AuthorsLamm K, Zajic G, Schacht J
JournalHear Res
PubMed ID7737932
The spiral modiolar artery with its proximal branches and the microvessels in the spiral ligament and the stria vascularis were microdissected from the guinea pig cochlea. After incubation with proteolytic and collagenolytic enzymes the mixed cell suspension was fractionated by gradient centrifugation. The cells migrated according to their buoyant densities ... More
[Ca2+]i and protein kinase C in vasopressin-induced prostacyclin and ANP release in rat cardiomyocytes.
AuthorsVan der Bent V, Church DJ, Vallotton MB, Meda P, Kem DC, Capponi AM, Lang U
JournalAm J Physiol
PubMed ID8141361
Exposure of cultured, spontaneously beating rat cardiomyocytes to arginine vasopressin (AVP) led to marked increases in the release of prostacyclin (PGI2) and atrial natriuretic peptide (ANP). These responses were accompanied by a rapid, transient rise of cytosolic free Ca2+ concentration ([Ca2+]i) and of membranous protein kinase C (PKC) activity. Ca2+ ... More
Ca2+- and phosphatidylinositol 3-kinase-dependent nitric oxide generation in lung endothelial cells in situ with ischemia.
AuthorsAl-Mehdi AB, Song C, Tozawa K, Fisher AB
JournalJ Biol Chem
PubMed ID11042195
Endothelial cells generate nitric oxide (NO) in response to agonist stimulation or increased shear stress. In this study, we evaluated the effects of abrupt cessation of shear stress on pulmonary endothelial NO generation and its relationship to changes in intracellular Ca(2+). In situ endothelial generation of NO and changes in ... More
Cytokine- and endotoxin-induced nitric oxide synthase in rat astroglial cultures: differential modulation by angiotensin II.
AuthorsKopnisky KL, Sumners C, Chandler LJ
JournalJ Neurochem
PubMed ID9048738
Recent studies have shown that the stimulatory effects of bacterial endotoxin [lipopolysaccharide (LPS)] on inducible nitric oxide (NO) synthase (iNOS) in astroglia are significantly reduced by the peptide angiotensin II (Ang II). In the present study we have compared the modulatory actions of Ang II on cytokine- and LPS-stimulated iNOS ... More
Slow degradation of aggregates of the Alzheimer's disease amyloid beta-protein by microglial cells.
AuthorsParesce DM, Chung H, Maxfield FR
JournalJ Biol Chem
PubMed ID9361021
Microglia are immune system cells associated with senile plaques containing beta-amyloid (Abeta) in Alzheimer's disease. Although microglia are an integral part of senile plaques, their role in the development of Alzheimer's disease is not known. Because microglia are phagocytic cells, it has been suggested that microglia may function as plaque-attacking ... More
Extracellular ATP activates a cation conductance and a K+ conductance in cultured microglial cells from mouse brain.
AuthorsWalz W, Ilschner S, Ohlemeyer C, Banati R, Kettenmann H
JournalJ Neurosci
PubMed ID7692013
Microglial cells have important functions during regenerative processes after brain injury. It is well established that they rapidly respond to damage to the brain tissue. Stages of activation are associated with changes of cellular properties such as proliferation rate or expression of surface antigens. Yet, nothing is known about signal ... More
Two-stage isolation procedure for obtaining homogenous populations of microvascular endothelial and mesothelial cells from human omentum.
AuthorsChung-Welch N, Patton WF, Shepro D, Cambria RP
JournalMicrovasc Res
PubMed ID9327383
The human omentum is a highly vascularized tissue often advocated as a source of human microvascular endothelial (HOME) cells. The omentum also contains mesothelial (MESO) cells and isolation protocols published to date do not describe a separation of the two cell populations. Using a two-stage collagenase digestion procedure, homogenous populations ... More
Membrane properties of ameboid microglial cells in the corpus callosum slice from early postnatal mice.
AuthorsBrockhaus J, Ilschner S, Banati RB, Kettenmann H
JournalJ Neurosci
PubMed ID8410196
Microglial cells in culture are distinct from neurons, macroglial cells, and macrophages of tissues other than brain with respect to their membrane current pattern. To assess these cells in the intact tissue, we have applied the patch-clamp technique to study membrane currents in microglial cells from acute, whole brain slices ... More
Phagocytosis of cholesteryl ester is amplified in diabetic mouse macrophages and is largely mediated by CD36 and SR-A.
Type 2 diabetes (T2D) is associated with accelerated atherosclerosis, which accounts for approximately 75% of all diabetes-related deaths. Here we investigate the link between diabetes and macrophage cholesteryl ester accumulation. When diabetic (db/db) mice are given cholesteryl ester intraperitoneally (IP), peritoneal macrophages (PerMPhis) recovered from these animals showed a 58% ... More
Age-related increase in the uptake of acetylated low density lipoprotein into cultured endothelial cells from rat aorta.
AuthorsHashimoto M, Ishinaga Y, Honda M, Ohoka M, Morioka S, Moriyama K
JournalExp Gerontol
PubMed ID1936198
A simple and reliable method for the separation of endothelial cells from the thoracic aorta of rats was established in the present study. The cultured endothelial cells separated by this improved explantation method showed typical characteristics of endothelial cells, morphology of cobblestone monolayer, immunoreactivity against antibodies to Von Willebrand's factor ... More
Endothelial barrier strengthening by activation of focal adhesion kinase.
AuthorsQuadri SK, Bhattacharjee M, Parthasarathi K, Tanita T, Bhattacharya J
JournalJ Biol Chem
PubMed ID12556538
Endothelial cell barrier (EC) properties regulate blood tissue fluid flux. To determine the role of endothelial-matrix interactions in barrier regulation, we induced cell shrinkage by exposing confluent endothelial monolayers to hyperosmolarity. The dominant effect of a 15-min hyperosmolar exposure was an increase in the trans-endothelial electrical resistance, indicating the induction ... More