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查看更多产品信息 LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry - FAQs (L34952)
5 个常见问题解答
有两种简单的方法。一种是通过60°C放置20分钟热灭活细胞。第二种是将细胞放入70%乙醇。乙醇固定的细胞可以在冰箱中无限期储存直到使用，可达好几年。
1.离心细胞、使细胞沉淀并去除上清
2.固定细胞：在15mL含有细胞团的试管中加入10 mL70%冰乙醇，先开始逐滴加入后震荡，混合均匀。
3.在冰箱中储存直到使用。
4.快使用时，水洗两次且在选择的缓冲液中重悬。
可以。流式细胞仪可用于：
•计数检测：用于流式细胞仪的细菌计数试剂盒（货号B7277）或LIVE/DEAD BacLight细菌活性和计数试剂盒（货号L34856）
•活性/活力检测：LIVE/DEAD BacLight 细菌活性试剂盒（货号 L13152）
•膜电位：BacLight细菌膜电位试剂盒（货号B34950）
•酵母活性/活力实验：用于流式细胞仪（货号L34952）的LIVE/DEAD FungaLight酵母活性试剂盒，FungaLight酵母CFDA, AM/碘化丙啶活力试剂盒（货号F34953）。
SYTO 9 dye: 480/500 nm
Propidium iodide: 490/635 nm
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.
Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes, you can. We offer:
-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.