LIVE/DEAD™ Violet 活力/生命力试剂盒，用于 405 nm 激发
LIVE/DEAD™ Violet 活力/生命力试剂盒，用于 405 nm 激发
Invitrogen™

LIVE/DEAD™ Violet 活力/生命力试剂盒，用于 405 nm 激发

The LIVE/DEAD™ Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous了解更多信息
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货号数量
L34958200 次检测
货号 L34958
价格（CNY)
6,801.00
飞享价
Ends: 31-Dec-2025
9,036.00
共减 2,235.00 (25%)
Each
添加至购物车
数量:
200 次检测
价格（CNY)
6,801.00
飞享价
Ends: 31-Dec-2025
9,036.00
共减 2,235.00 (25%)
Each
添加至购物车
The LIVE/DEAD™ Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.

View a selection guide for all Nonfixable Viability Dyes for Flow Cytometry.
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性不透过
细胞类型真核细胞
描述LIVE/DEAD™ Violet 活力/生命力试剂盒，用于 405 nm 激发
检测方法荧光
染料类型其他标记或染料
形式冻干
产品规格管
数量200 次检测
运输条件室温
溶解度DMSO（二甲亚砜）
颜色浅绿色、紫色
发射浅绿色荧光反应性染料：367/526 nm
钙黄绿素紫色染色剂：400/452 nm
Excitation Wavelength Range367/400 nm
适用于（应用）活力测定试剂盒
适用于（设备）流式细胞仪
产品线LIVE/DEAD™
产品类型染色剂
Unit SizeEach
内容与储存
包含5小瓶钙黄绿素紫色染色剂（25 μg/瓶）、5小瓶浅绿色荧光反应染料和1小瓶 DMSO。

在冷柜（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

我如何为LIVE/DEAD细胞活力实验准备死细胞对照？

有两种简单的方法。一种是通过60°C放置20分钟热灭活细胞。第二种是将细胞放入70%乙醇。乙醇固定的细胞可以在冰箱中无限期储存直到使用，可达好几年。

1.离心细胞、使细胞沉淀并去除上清
2.固定细胞：在15mL含有细胞团的试管中加入10 mL70%冰乙醇，先开始逐滴加入后震荡，混合均匀。
3.在冰箱中储存直到使用。
4.快使用时，水洗两次且在选择的缓冲液中重悬。

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.