Endothelial and smooth muscle cell conduction in arterioles controlling blood flow.
AuthorsWelsh DG, Segal SS
JournalAm J Physiol
PubMed ID9458866
We performed intracellular recording with Lucifer yellow dye microinjection to investigate the cellular pathway(s) by which constriction and dilation are conducted along the wall of arterioles (diameter 47 +/- 1 microns, n = 63) supplying blood flow to the cheek pouch of anesthetized hamsters. At rest, membrane potential (Em) of ... More
Bursting response to current-evoked depolarization in rat CA1 pyramidal neurons is correlated with lucifer yellow dye coupling but not with the presence of calbindin-D28k.
AuthorsBaimbridge KG, Peet MJ, McLennan H, Church J
JournalSynapse
PubMed ID2042109
Calbindin-D28k (CaBP) immunohistochemistry has been combined with electrophysiological recording and Lucifer Yellow (LY) cell identification in the CA1 region of the rat hippocampal formation. CaBP is shown to be contained within a distinct sub-population of CA1 pyramidal cells which is equivalent to the superficial layer described by Lorente de Nó ... More
Membrane conductance oscillations in astrocytes induced by phorbol ester.
AuthorsMacVicar BA, Crichton SA, Burnard DM, Tse FW
JournalNature
PubMed ID3627267
Glial cells in the central nervous systems (CNS) have complex functions which are difficult to decipher because of the intimate intertwining of glial cells with neurons. We have therefore developed an essentially neuron-free preparation of CNS astrocytes in the kainic acid lesioned hippocampal slice. With this preparation we have examined ... More
Deformation of network connectivity in the inferior olive of connexin 36-deficient mice is compensated by morphological and electrophysiological changes at the single neuron level.
AuthorsDe Zeeuw CI, Chorev E, Devor A, Manor Y, Van Der Giessen RS, De Jeu MT, Hoogenraad CC, Bijman J, Ruigrok TJ, French P, Jaarsma D, Kistler WM, Meier C, Petrasch-Parwez E, Dermietzel R, Sohl G, Gueldenagel M, Willecke K, Yarom Y
JournalJ Neurosci
PubMed ID12805309
Compensatory mechanisms after genetic manipulations have been documented extensively for the nervous system. In many cases, these mechanisms involve genetic regulation at the transcription or expression level of existing isoforms. We report a novel mechanism by which single neurons compensate for changes in network connectivity by retuning their intrinsic electrical ... More
Dual fluorescence combined with a two-color immunoperoxidase technique: a new way of visualizing diverse neuronal elements.
AuthorsPilowksy PM, Lipski J, Prestidge R, Jiang C
JournalJ Neurosci Methods
PubMed ID1712061
'A method is described that allows an estimation of the neurotransmitter-related immunoreactivity, morphology and relationship to other immunoreactive elements, of single functionally identified neurons in the central nervous system. First, neurons are identified electrophysiologically using intracellular recording and labelled by iontophoresis of lucifer yellow (LY). After fixation and sectioning of ... More
Role of the small GTPase Rab7 in the late endocytic pathway.
AuthorsVitelli R, Santillo M, Lattero D, Chiariello M, Bifulco M, Bruni CB, Bucci C
JournalJ Biol Chem
PubMed ID9020161
'Rab7 is a small GTPase localized to the late endosomal compartment. Its function was investigated by overexpressing dominant negative or constitutively active mutants in BHK-21 cells. The effects of such overexpression on the internalization and/or degradation of different endocytic markers and on the morphology of the late endosomal compartment were ... More
Intracellular dye injection of previously immunolabeled insect neurons in fixed brain slices.
AuthorsWegerhoff R, Breidbach O
JournalJ Neurosci Methods
PubMed ID7990518
'Our method combines intracellular dye injection and immunohistochemistry. Under optical control, Lucifer Yellow was injected into immunohistochemically identified neurons that reside in fixed tissue. The technique allows visualization of the complete arborization patterns of immunostained neurons. Injections were performed on small neurons (somata < 10 microns in diameter). The technique ... More
Specific sterols required for the internalization step of endocytosis in yeast.
AuthorsMunn AL, Heese-Peck A, Stevenson BJ, Pichler H, Riezman H
JournalMol Biol Cell
PubMed ID10564282
'Sterols are major components of the plasma membrane, but their functions in this membrane are not well understood. We isolated a mutant defective in the internalization step of endocytosis in a gene (ERG2) encoding a C-8 sterol isomerase that acts in the late part of the ergosterol biosynthetic pathway. In ... More
Structure and function of retinal ganglion cells innervating the cat's geniculate wing: an in vitro study.
AuthorsPu M, Berson DM, Pan T
JournalJ Neurosci
PubMed ID8027783
'We have examined in vitro the morphology and visual response properties of retinal ganglion cells innervating a component of the cat's lateral geniculate nucleus known as the geniculate wing (or retinorecipient zone of the pulvinar). Ganglion cells were first labeled in situ by retrograde transport of fluorescent microspheres from the ... More
Semaphorin II can function as a selective inhibitor of specific synaptic arborizations.
'Previous studies showed that grasshopper semaphorin I, a transmembrane semaphorin, functions in vivo to steer a pair of growth cones, prevent defasciculation, and inhibit branching; and that chick collapsin, a secreted semaphorin, can function in vitro to cause growth cone collapse. Semaphorin II, a secreted semaphorin in Drosophila, is transiently ... More
An electron-microscopic study of smooth muscle cell dye coupling in the pig coronary arteries. Role of gap junctions.
AuthorsBény JL, Connat JL
JournalCirc Res
PubMed ID1309317
'Arterial smooth muscles behave like a syncytium, since they are electrically coupled. It is generally assumed that electrical coupling and dye coupling are mediated by gap junctions. No gap junctions could be detected by transmission electron microscopy in media of coronary arteries. We looked for the presence of gap junction ... More
The orbital and medial prefrontal circuit through the primate basal ganglia.
AuthorsHaber SN, Kunishio K, Mizobuchi M, Lynd-Balta E
JournalJ Neurosci
PubMed ID7623116
'The ventral striatum is considered an interface between limbic and motor systems. We followed the orbital and medial prefrontal circuit through the monkey basal ganglia by analyzing the projection from this cortical area to the ventral striatum and the representation of orbitofrontal cortex via the striatum, in the globus pallidus ... More
Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques.
AuthorsMusil LS, Goodenough DA
JournalJ Cell Biol
PubMed ID1659577
'We previously demonstrated that the gap junction protein connexin43 is translated as a 42-kD protein (connexin43-NP) that is efficiently phosphorylated to a 46,000-Mr species (connexin43-P2) in gap junctional communication-competent, but not in communication-deficient, cells. In this study, we used a combination of metabolic radiolabeling and immunoprecipitation to investigate the assembly ... More
Antiserum to lucifer yellow: preparation, characterization, and use for immunocytochemical localization of dye-filled retinal neurons.
AuthorsBrandon C, Criswell MH
JournalJ Histochem Cytochem
PubMed ID1918929
'We present a new method for the preparation of antisera to Lucifer Yellow, and these antisera are here shown to be particularly suitable for immunocytochemical localization of multiple dye-injected cells in large pieces of vertebrate retina. The method involves the preparation of covalent conjugates of the VS isomer of Lucifer ... More
Synaptic vesicle proteins and early endosomes in cultured hippocampal neurons: differential effects of Brefeldin A in axon and dendrites.
AuthorsMundigl O, Matteoli M, Daniell L, Thomas-Reetz A, Metcalf A, Jahn R, De Camilli P
JournalJ Cell Biol
PubMed ID8376458
'The pathways of synaptic vesicle (SV) biogenesis and recycling are still poorly understood. We have studied the effects of Brefeldin A (BFA) on the distribution of several SV membrane proteins (synaptophysin, synaptotagmin, synaptobrevin, p29, SV2 and rab3A) and on endosomal markers to investigate the relationship between SVs and the membranes ... More
Sequential changes of gap-junctional intercellular communications during multistage rat liver carcinogenesis: direct measurement of communication in vivo.
AuthorsKrutovskikh VA, Oyamada M, Yamasaki H
JournalCarcinogenesis
PubMed ID1893531
'We have developed a simple method to measure gap-junctional intercellular communication (GJIC), by means of microinjection/dye transfer assay, in liver slices freshly removed from the rat. Using this method and immunostaining of connexin 32 (cx32), the major liver gap junction protein, we studied sequential changes of GJIC during chemical hepatocarcinogenesis ... More
Fibroblast growth factor 4 directs gap junction expression in the mesenchyme of the vertebrate limb Bud.
AuthorsMakarenkova H, Becker DL, Tickle C, Warner AE
JournalJ Cell Biol
PubMed ID9281589
'Pattern in the developing limb depends on signaling by polarizing region mesenchyme cells, which are located at the posterior margin of the bud tip. Here we address the underlying cellular mechanisms. We show in the intact bud that connexin 43 (Cx43) and Cx32 gap junctions are at higher density between ... More
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
AuthorsSoldani C, Croce AC, Bottone MG, Fraschini A, Biggiogera M, Bottiroli G, Pellicciari C,
JournalHistochem Cell Biol
PubMed ID17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored ... More
Physiological role of gap-junctional hemichannels. Extracellular calcium-dependent isosmotic volume regulation.
AuthorsQuist AP, Rhee SK, Lin H, Lal R
JournalJ Cell Biol
PubMed ID10704454
'Hemichannels in the overlapping regions of apposing cells plasma membranes join to form gap junctions and provide an intercellular communication pathway. Hemichannels are also present in the nonjunctional regions of individual cells and their activity is gated by several agents, including calcium. However, their physiological roles are unknown. Using techniques ... More
Kinesin-mediated organelle translocation revealed by specific cellular manipulations.
AuthorsFeiguin F, Ferreira A, Kosik KS, Caceres A
JournalJ Cell Biol
PubMed ID7962067
'The distribution of membrane-bound organelles was studied in cultured hippocampal neurons after antisense oligonucleotide suppression of the kinesin-heavy chain (KHC). We observed reduced 3,3''-dihexyloxacarbocyanine iodide (DiOC6(3)) fluorescent staining in neurites and growth cones. In astrocytes, KHC suppression results in the disappearance of the DiOC6(3)-positive reticular network from the cell periphery, ... More
Lessons from loricrin-deficient mice: compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein.
AuthorsKoch PJ, de Viragh PA, Scharer E, Bundman D, Longley MA, Bickenbach J, Kawachi Y, Suga Y, Zhou Z, Huber M, Hohl D, Kartasova T, Jarnik M, Steven AC, Roop DR
JournalJ Cell Biol
PubMed ID11038185
'The epidermal cornified cell envelope (CE) is a complex protein-lipid composite that replaces the plasma membrane of terminally differentiated keratinocytes. This lamellar structure is essential for the barrier function of the skin and has the ability to prevent the loss of water and ions and to protect from environmental hazards. ... More
Importance of MEK in neutrophil microbicidal responsiveness.
'Exposure of neutrophils to inflammatory stimuli such as the chemoattractant FMLP leads to activation of responses including cell motility, the oxidative burst, and secretion of proteolytic enzymes. A signaling cascade involving sequential activation of Raf-1, mitogen-activated protein kinase (MEK), and extracellular signal regulated kinase (ERK) is also rapidly activated after ... More
Neuronal coupling in the developing mammalian retina.
AuthorsPenn AA, Wong RO, Shatz CJ
JournalJ Neurosci
PubMed ID8207489
'During the first 3 weeks of postnatal development in the ferret retina, cells in the ganglion cell layer spontaneously generate waves of electrical activity that travel across the retina in the absence of mature photoreceptors (Meister et al., 1991; Wong et al., 1993). Since few chemical synapses are present at ... More
Dissection of the molecular basis of pp60(v-src) induced gating of connexin 43 gap junction channels.
AuthorsZhou L, Kasperek EM, Nicholson BJ
JournalJ Cell Biol
PubMed ID10085299
'Suppression of gap-junctional communication by various protein kinases, growth factors, and oncogenes frequently correlates with enhanced mitogenesis. The oncogene v-src appears to cause acute closure of gap junction channels. Tyr265 in the COOH-terminal tail of connexin 43 (Cx43) has been implicated as a potential target of v-src, although v-src action ... More
Rod-signal interneurons in the rabbit retina: 2. AII amacrine cells.
AuthorsVaney DI, Gynther IC, Young HM
JournalJ Comp Neurol
PubMed ID1955580
'AII amacrine cells, which are the third-order neurons in the rod pathway, can be differentially labelled in rabbit retina by injecting Nuclear Yellow into the posterior chamber. Under ultraviolet excitation, the labelled retina appears strongly metachromatic, with the AII nuclei fluorescing silvery-yellow and the nuclei of other amacrine cells fluorescing ... More
Postnatal development of dye-coupling among astrocytes in rat visual cortex.
AuthorsBinmöller FJ, Müller CM
JournalGlia
PubMed ID1328051
'Intercellular coupling among astrocytes was studied in rat visual cortex slices from animals aged 1 week to 4 months. Cell coupling via gap junctions was determined by the dye spread of the low molecular weight dye Lucifer Yellow CH injected into electrophysiologically identified cells to adjacent cells. Coupling among glial ... More
Phorbol esters stimulate macropinocytosis and solute flow through macrophages.
AuthorsSwanson JA
JournalJ Cell Sci
PubMed ID2613767
'The morphology and kinetics of pinocytosis by bone marrow-derived macrophages were studied to determine how stimulation by phorbol esters increases net solute accumulation. Application of phorbol myristate acetate (PMA) increased both the abundance of macropinosomes and the rate of solute flow through the endocytic compartment. The large pinosomes originated as ... More
A system of indoleamine-accumulating neurons in the rabbit retina.
AuthorsSandell JH, Masland RH
JournalJ Neurosci
PubMed ID3772435
'The indoleamine-accumulating neurons of the rabbit retina were labeled by intraocular injection of 5,7-dihydroxytryptamine (5,7-DHT). The retinas were fixed with 2.5% paraformaldehyde and 0.2% glutaraldehyde and inspected by fluorescence microscopy. Five kinds of cell accumulated the indoleamine. They were labeled to essentially the same brightness and remained so despite variations ... More
Retrograde transport of sodium selenite and intracellular injection of micro-ruby: a combined method to describe the morphology of zinc-rich neurones.
AuthorsMiró-Bernié N, Sancho-Bielsa FJ, López-García C, Pérez-Clausell J
JournalJ Neurosci Methods
PubMed ID12906949
'Zinc is found in synaptic vesicles in a large number of glutamatergic systems. Its involvement in neurotransmission and neurological disorders has been suggested. There are methods for tracing these circuits, but they do not fill the dendritic tree. In this study, extracellular selenite injections in vivo were combined with intracellular ... More
Dynamics from a time series: can we extract the phase resetting curve from a time series?
AuthorsOprisan SA, Thirumalai V, Canavier CC
JournalBiophys J
PubMed ID12719224
'Recordings of the membrane potential from a bursting neuron were used to reconstruct the phase curve for that neuron for a limited set of perturbations. These perturbations were inhibitory synaptic conductance pulses able to shift the membrane potential below the most hyperpolarized level attained in the free running mode. The ... More
Connexin26 is responsible for anionic molecule permeability in the cochlea for intercellular signalling and metabolic communications.
AuthorsZhao HB
JournalEur J Neurosci
PubMed ID15869481
'Abstract A gap junction is composed of two hemichannels and possesses a relatively large pore size ( approximately 10-15 A), allowing passage of ions and molecules up to 1 kDa. Here, we report that connexin hemichannels and gap junctions in the guinea pig cochlea had significant charge selectivity among permeating ... More
Electrical properties of iridial arterioles of the rat.
AuthorsHirst GD, Edwards FR, Gould DJ, Sandow SL, Hill CE
JournalAm J Physiol
PubMed ID9374786
'When intracellular recordings were made from iridial arterioles, the cells had membrane potentials of about -65 mV and perivascular nerve stimulation evoked a membrane depolarization. When these cells were labeled with lucifer yellow, all cells that responded to perivascular nerve stimulation had the morphological characteristics of smooth muscle cells. Cells ... More
Patch-clamp recordings from white matter glia in thin longitudinal slices of adult rat spinal cord.
'We developed a technique of whole cell patch-clamp recordings from white matter oligodendrocytes and astrocytes in 200-250 microm-thick horizontal slices of adult (>2 months, 240-260 g) rat thoracic spinal cord. The viability of the white matter, sectioned in Na(+)-free, low Ca(2+) media, and the function of axons were preserved for ... More
Local circuitry of identified projection neurons in cat visual cortex brain slices.
AuthorsKatz LC
JournalJ Neurosci
PubMed ID3553446
'The relationship between pyramidal cell morphology and efferent target was investigated in layer 6 of cat primary visual cortex (area 17). Layer 6 has 2 projections, one to the lateral geniculate nucleus (LGN) and another to the visual claustrum. The cells of origin of each projection were identified by retrograde ... More
A prelysosomal compartment sequesters membrane-impermeant fluorescent dyes from the cytoplasmic matrix of J774 macrophages.
AuthorsSteinberg TH, Swanson JA, Silverstein SC
JournalJ Cell Biol
PubMed ID3417786
'After the membrane impermeant dye Lucifer Yellow is introduced into the cytoplasmic matrix of J774 cells, the dye is sequestered within cytoplasmic vacuoles and secreted into the extracellular medium. In the present work we studied the intracellular transport of Lucifer Yellow in J774 macrophages and the nature of the cytoplasmic ... More
Cell-cell interactions in the process of differentiation of thyroid epithelial cells into follicles: a study by microinjection and fluorescence microscopy on in vitro reconstituted thyroid follicles.
AuthorsMunari-Silem Y, Mesnil M, Selmi S, Bernier-Valentin F, Rabilloud R, Rousset B
JournalJ Cell Physiol
PubMed ID2125602
'Thyroid cells, cultured in the presence of thyroid stimulating hormone, reorganized within 36-48 hr into follicular structures, the in vitro reconstituted thyroid follicles or RTF. By microinjection of fluorescent probes either into the neoformed intrafollicular lumen (IL) or into cells forming the follicles, we have studied the development and some ... More
Anatomical and physiological localization of prelabeled grafts in rat hippocampus.
AuthorsPyapali GK, Turner DA, Madison RD
JournalExp Neurol
PubMed ID1577121
'Dissociated rat fetal hippocampal cells were grafted into normal adult rats. The fetal cells were incubated with one of a number of fluorescent compounds at the time of the dissociation to facilitate identification of the individual grafted cells. The fluorescent labels which were analyzed for this purpose included rhodamine latex ... More
Simultaneous localization and quantification of relative G and F actin content: optimization of fluorescence labeling methods.
AuthorsKnowles GC, McCulloch CA
JournalJ Histochem Cytochem
PubMed ID1527379
'Previous studies of fluorescence probes for labeling the monomeric actin pool have demonstrated lack of specificity. We have used quantitative analytical methods to assess the sensitivity and specificity of rhodamine DNAse I as a probe for monomeric (G) actin. The G-actin pool of attached or suspended fibroblasts was stabilized by ... More
Synaptic responses evoked by mechanical stimulation of the mucosa in morphologically characterized myenteric neurons of the guinea-pig ileum.
AuthorsBornstein JC, Furness JB, Smith TK, Trussell DC
JournalJ Neurosci
PubMed ID1846909
'Recordings were made from myenteric neurons of the guinea-pig ileum during reflexes evoked by mechanical stimulation of the mucosa. Impaled neurons were injected with dye (Lucifer yellow or biocytin), and their shapes were determined. All neurons were 5-12 mm from the stimulus, a brush stroke that deformed the mucosal villi. ... More
Fluorescent gangliosides as probes for the retention and organization of fibronectin by ganglioside-deficient mouse cells.
AuthorsSpiegel S, Yamada KM, Hom BE, Moss J, Fishman PH
JournalJ Cell Biol
PubMed ID3882721
'Ganglioside-deficient transformed mouse fibroblasts (NCTC 2071A cells), which grow in serum-free medium, synthesize fibronectin but do not retain it on the cell surface. When fluorescent derivatives of gangliosides, containing either rhodamine or Lucifer yellow CH attached to the sialic acid residues, were added to the culture medium, the cells incorporated ... More
Labeling of pyramidal and nonpyramidal neurons with lectin Vicia villosa during postnatal development of the guinea pig.
AuthorsOhyama J, Ojima H
JournalJ Comp Neurol
PubMed ID9414006
'Labeling of cortical neurons with a lectin, Vicia villosa (VVA), was investigated in guinea pigs aged 1 day old to adult. Lectin histochemistry revealed a perineuronal sheath, which outlined the cell bodies, apical dendrites, and axon initial segments, in distinct populations of pyramidal and nonpyramidal neurons. Their laminar positions were ... More
The postnatal development of layer VI pyramidal neurons in the cat's striate cortex, as visualized by intracellular Lucifer yellow injections in aldehyde-fixed tissue.
AuthorsLübke J, Albus K
JournalBrain Res Dev Brain Res
PubMed ID2917410
'The postnatal development of layer VI pyramidal neurons in the cat''s striate cortex has been studied by means of intracellular injections of Lucifer yellow in aldehyde-fixed tissue (LYF technique). It is shown that the LYF technique gives results qualitatively and quantitatively similar to results obtained with other techniques (Golgi, marker-injections ... More
Gap junctional communication of primary human keratinocytes: characterization by dual voltage clamp and dye transfer.
AuthorsSalomon D, Chanson M, Vischer S, Masgrau E, Vozzi C, Saurat JH, Spray DC, Meda P
JournalExp Cell Res
PubMed ID1639140
'We have compared dye coupling in pairs of small (less than 10 microns in diameter) and large (greater than 20 microns in diameter) keratinocytes isolated from normal human epidermis, using Lucifer yellow microinjection. Under control conditions, dye coupling was found in only 1 out of the 25 small pairs tested, ... More
Dopamine enhances a glutamate-gated ionic current in OFF bipolar cells of the tiger salamander retina.
AuthorsMaguire G, Werblin F
JournalJ Neurosci
PubMed ID7931565
'The transmitter glutamate is thought to be used by all vertebrate photoreceptors to drive the second-order neurons of the retina, horizontal and bipolar neurons. Dopamine, an endogenous retinal neurotransmitter localized to amacrine and interplexiform cells, has previously been shown to enhance glutamate-gated currents in retinal horizontal cells. In the present ... More
Signaling at the gliovascular interface.
AuthorsSimard M, Arcuino G, Takano T, Liu QS, Nedergaard M
JournalJ Neurosci
PubMed ID14534260
'Advances in fluorescent calcium indicating dyes over the past decade have identified calcium signaling as the tool by which astrocytes communicate among themselves and with neighboring neurons. Studies of astrocyte-neuron interactions have shown that calcium signaling is a potent modulator of the strength of both excitatory and inhibitory synapses. The ... More
Quantitative determination of gap junction intercellular communication by scrape loading and image analysis.
AuthorsOpsahl H, Rivedal E
JournalCell Adhes Commun
PubMed ID10830616
'Gap junction intercellular communication (GJIC) consists of intercellular exchange of low molecular weight molecules. Chemically induced alterations of this communication have been suggested to result in abnormal cell growth and tumour promotion. Several in vitro assays have been developed to determine the effect of chemicals on gap junction communication in ... More
Lack of bradykinin-induced smooth muscle cell hyperpolarization despite heterocellular dye coupling and endothelial cell hyperpolarization in porcine ciliary artery.
AuthorsBény JL, Zhu P, Haefliger IO
JournalJ Vasc Res
PubMed ID9349728
'In porcine coronary artery, bradykinin-induced endothelium-dependent vasodilatations are associated with simultaneous endothelium as well as endothelium-dependent smooth muscle cell (SMC) hyperpolarizations. In contrast, in porcine ciliary artery bradykinin evokes endothelium-dependent relaxations, but no change in SMC membrane potential. This study addresses the question of whether the lack of bradykinin-induced SMC ... More
Inhibition of lipid peroxidation and structure-activity-related studies of the dietary constituents anthocyanins, anthocyanidins, and catechins.
AuthorsSeeram NP, Nair MG
JournalJ Agric Food Chem
PubMed ID12207466
'The antioxidant activities of a series of commonly consumed and biogenetically related plant phenolics, namely, anthocyanidins, anthocyanins, and catechins, in a liposomal model system have been investigated. The antioxidant efficacies of the compounds were evaluated on their abilities to inhibit the fluorescence intensity decay of an extrinsic probe, 3-[p-(6-phenyl)-1,3,5-hexatrienyl]phenylpropionic acid, ... More
Communication compartments in the gastrulating mouse embryo.
AuthorsKalimi GH, Lo CW
JournalJ Cell Biol
PubMed ID3392100
'We characterized the pattern of gap junctional communication in the 7.5-d mouse embryo (at the primitive streak or gastrulation stage). First we examined the pattern of dye coupling by injecting the fluorescent tracers, Lucifer Yellow or carboxyfluorescein, and monitoring the extent of dye spread. These studies revealed that cells within ... More
Pep7p provides a novel protein that functions in vesicle-mediated transport between the yeast Golgi and endosome.
AuthorsWebb GC, Zhang J, Garlow SJ, Wesp A, Riezman H, Jones EW
JournalMol Biol Cell
PubMed ID9168472
'Saccharomyces cerevisiae pep7 mutants are defective in transport of soluble vacuolar hydrolases to the lysosome-like vacuole. PEP7 is a nonessential gene that encodes a hydrophilic protein of 515 amino acids. A cysteine-rich tripartite motif in the N-terminal half of the polypeptide shows striking similarity to sequences found in many other ... More
Acidification of the cytosol inhibits endocytosis from coated pits.
AuthorsSandvig K, Olsnes S, Petersen OW, van Deurs B
JournalJ Cell Biol
PubMed ID2887575
'Acidification of the cytosol of a number of different cell lines strongly reduced the endocytic uptake of transferrin and epidermal growth factor. The number of transferrin binding sites at the cell surface was increased in acidified cells. Electron microscopic studies showed that the number of coated pits at the cell ... More
Cell-to-cell diffusion of fluorescent dyes in paired ventricular cells.
AuthorsImanaga I, Kameyama M, Irisawa H
JournalAm J Physiol
PubMed ID3812712
'The intracellular and cell-to-cell diffusion of fluorescent dyes of various molecular sizes were studied in enzymatically isolated paired ventricular cells of the guinea pig heart. Fluorescein sodium (mol wt 332), 6-carboxyfluorescein (mol wt 376), Lucifer yellow CH (mol wt 457), lissamine rhodamine B-200 (mol wt 559), and tetraglycine-conjugated lissamine rhodamine ... More
Bystander tumoricidal effect and gap junctional communication in lung cancer cell lines.
AuthorsImaizumi K, Hasegawa Y, Kawabe T, Emi N, Saito H, Naruse K, Shimokata K
JournalAm J Respir Cell Mol Biol
PubMed ID9476907
'Tumor cells expressing the herpes simplex virus-thymidine kinase (HSV-tk) gene become sensitive to ganciclovir (GCV), and the phenomenon by which tumor cells surrounding the HSV-tk expressing cells also become sensitive to GCV is known as the "bystander effect." The purpose of this study was to investigate the bystander effect in ... More
Functional consequences of compartmentalization of synaptic input.
AuthorsColeman MJ, Nusbaum MP
JournalJ Neurosci
PubMed ID7965058
'Intra-axonal recordings of stomatogastric nerve axon 1 (SNAX1) indicate that there are synaptic inputs onto the SNAX1 terminals in the stomatogastric ganglion (STG) of the crab Cancer borealis (Nusbaum et al., 1992b). To determine whether this synaptic input only influenced SNAX1 activity within the STG, we identified the SNAX1 soma ... More
Cellular heterogeneity in cerebral cortex: a study of the morphology of pyramidal neurones in visual areas of the marmoset monkey.
AuthorsElston GN, Tweedale R, Rosa MG
JournalJ Comp Neurol
PubMed ID10540356
'The morphological characteristics of the basal dendritic fields of layer III pyramidal neurones were determined in visual areas in the occipital, parietal, and temporal lobes of adult marmoset monkeys by means of intracellular iontophoretic injection of Lucifer yellow. Neurones in the primary visual area (V1) had the least extensive and ... More
Photoinactivation of neurones axonally filled with the fluorescent dye 5(6)-carboxyfluorescein in the pond snail, Lymnaea stagnalis.
AuthorsKemenes G, Daykin K, Elliott CJ
JournalJ Neurosci Methods
PubMed ID1787741
'We describe a new, simple and reliable technique to fill molluscan neurones from their cut axons with sufficient fluorescent dye for photoinactivation experiments. The fluorescent dye 5(6)-carboxyfluorescein (5-CF) travels quickly up the nerves of the gastropod mollusc, Lymnaea stagnalis into the buccal ganglia and fills the cell bodies in 1-3 ... More
Actin-dependent fluid-phase endocytosis in inner cortex cells of maize root apices.
AuthorsBaluska F, Samaj J, Hlavacka A, Kendrick-Jones J, Volkmann D
JournalJ Exp Bot
PubMed ID14739268
'The fluorescent dye Lucifer Yellow (LY) is a well-known and widely-used marker for fluid-phase endocytosis. In this paper, both light and electron microscopy revealed that LY was internalized into transition zone cells of the inner cortex of intact maize root apices. The internalized LY was localized within tubulo-vesicular compartments invaginating ... More
Variation among acetylcholine receptor clusters induced by ciliary ganglion neurons in vitro.
AuthorsDubinsky JM, Morgan M, Fischbach GD
JournalDev Biol
PubMed ID2846386
'We have examined the variation in receptor density and area among neurite-associated acetylcholine receptor patches (NARPs) induced by chick ciliary ganglion neurons on nearby myotubes in vitro. Quantitative analysis of rhodamine-alpha-bungarotoxin (RBTX) NARPs revealed that about 15% of the NARPs were "outstanding" in terms of size (greater than 60 micron ... More
Inhibition of endosome function in CHO cells bearing a temperature-sensitive defect in the coatomer (COPI) component epsilon-COP.
AuthorsDaro E, Sheff D, Gomez M, Kreis T, Mellman I
JournalJ Cell Biol
PubMed ID9412469
'Recent evidence has suggested that subunits of the coatomer protein (COPI) complexes are functionally associated with endosomes in mammalian cells. We now provide genetic evidence that COPI plays a role in endocytosis in intact cells. The ldlF mutant CHO cell line bears a temperature-sensitive defect in the COPI subunit epsilon-COP. ... More
A simple method for embedding small specimens for photomicrography and sectioning following intracellular microiontophoresis of lucifer yellow CH.
AuthorsJones CJ
JournalHistochem J
PubMed ID2426220
'A simple method for the rapid processing of small specimens following intracellular labelling with the fluorescent naphthalimide dye Lucifer Yellow CH is described which involves embedding in glycol methacrylate-based resin on cavity slides. The technique, which may be suitable for other intracellular and extracellular fluorescent markers, permits early fluorescence photomicrography ... More
Transport of fluorescent phospholipid analogues from the erythrocyte membrane to the parasite in Plasmodium falciparum-infected cells.
AuthorsHaldar K, de Amorim AF, Cross GA
JournalJ Cell Biol
PubMed ID2661561
'The asexual development of the human malaria parasite Plasmodium falciparum is largely intraerythrocytic. When 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazole-4-yl)amino]caproyl] phosphatidylcholine (NBD-PC) was incorporated into infected and uninfected erythrocyte membranes at 0 degrees C, it remained at the cell surface. At 10 degrees C, the lipid was rapidly internalized in infected erythrocytes at all stages ... More
Cell-cell interactions during the migration of an identified commissural growth cone in the embryonic grasshopper.
AuthorsMyers PZ, Bastiani MJ
JournalJ Neurosci
PubMed ID8423467
'One of the fascicles of the posterior commissure of the embryonic grasshopper is pioneered by an individually identifiable neuron named Q1. Q1 initially grows along a longitudinal pathway established by another pioneer neuron, MP1, and then crosses to the midline, where it meets and fasciculates with the axon of the ... More
Hyaluronan and the hyaluronan receptor RHAMM promote focal adhesion turnover and transient tyrosine kinase activity.
AuthorsHall CL, Wang C, Lange LA, Turley EA
JournalJ Cell Biol
PubMed ID7518470
'The molecular mechanisms whereby hyaluronan (HA) stimulates cell motility was investigated in a C-H-ras transformed 10T 1/2 fibroblast cell line (C3). A significant (p < 0.001) stimulation of C3 cell motility with HA (10 ng/ml) was accompanied by an increase in protein tyrosine phosphorylation as detected by anti-phosphotyrosine antibodies using ... More
Biotin staining in the giant fiber systems of the lobster.
AuthorsMa PM
JournalJ Comp Neurol
PubMed ID7515403
'The avidin-biotin-complex method is a popular immunocytochemical technique. This method labels consistently a group of neurons in the lobster ventral nerve cord in the absence of primary antibodies. The specific staining is due to a relatively high level of endogenous biotin (or biocytin) in these neurons. These biotin-positive neurons are ... More
Initiation and propagation of regenerative Ca(2+)-dependent potentials in dendrites of layer 5 pyramidal neurons.
AuthorsOakley JC, Schwindt PC, Crill WE
JournalJ Neurophysiol
PubMed ID11431528
'The initiation and propagation of dendritic Ca(2+)-dependent regenerative potentials (CDRPs) were investigated by imaging the Ca(2+)-sensitive dye Fluo-4 during whole cell recording from the soma of layer 5 pyramidal neurons visualized in a slice preparation of rat neocortex by the use of infrared-differential interference contrast microscopy. CDRPs were evoked by ... More
Number, distribution, and morphology of retinal ganglion cells with axons regenerated into peripheral nerve graft in adult cats.
AuthorsWatanabe M, Sawai H, Fukuda Y
JournalJ Neurosci
PubMed ID8478691
'The optic nerve of adult cats was transected and an autologous peripheral nerve was transplanted to the stump. Two months later retinal ganglion cells (RGCs) that had regenerated their axons were retrogradely labeled with fluorescent dye injected into the graft. The more than 4000 labeled cells, which were 3.0-4.3% of ... More
Fluid flow increases membrane permeability to merocyanine 540 in human endothelial cells.
AuthorsBerthiaume F, Frangos JA
JournalBiochim Biophys Acta
PubMed ID8155677
'Fluid shear stress is a ubiquitous stimulus of mammalian cell metabolism; however, its signal transduction pathway is unknown. We hypothesized that shear stress may alter some physical properties of the cell membrane. Using primary human umbilical vein endothelial cells (HUVECs), we investigated the effects of shear on the cell membrane ... More
A versatile means of intracellular labeling: injection of biocytin and its detection with avidin conjugates.
AuthorsHorikawa K, Armstrong WE
JournalJ Neurosci Methods
PubMed ID3146670
'Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Since the latter molecule has been conjugated to several histochemical markers, the use of biocytin as an intracellular marker was investigated. Electrodes were filled with a solution of 4-6% biocytin ... More
Modulation of gap junctional mechanisms during calcium-free induced field burst activity: a possible role for electrotonic coupling in epileptogenesis.
AuthorsPerez-Velazquez JL, Valiante TA, Carlen PL
JournalJ Neurosci
PubMed ID8027781
'To date, there is little experimental evidence supporting or refuting electrotonic interactions through gap junctions in the generation and/or spread of seizure activity in the mammalian brain. We have studied gap junctional mechanisms in the in vitro calcium-free induced model of epilepsy using electrophysiological and staining techniques in the CA1 ... More
Spontaneous and stimulated firing in cultured rat suprachiasmatic neurons.
AuthorsWalsh IB, van den Berg RJ, Marani E, Rietveld WJ
JournalBrain Res
PubMed ID1382804
'Neurons from the suprachiasmatic nucleus (SCN) of the hypothalamus, the site of a circadian pacemaker in mammals, were isolated from embryonic rat. After mechanical dissociation neurons were brought into culture for 1-2 weeks, using a chemically defined medium. Recordings were made from 74 bipolar neurons using two different configurations of ... More
Interactions of pluronic block copolymers with brain microvessel endothelial cells: evidence of two potential pathways for drug absorption.
AuthorsMiller DW, Batrakova EV, Waltner TO, Alakhov VYu Kabanov AV
JournalBioconjug Chem
PubMed ID9327127
'Pluronic block copolymers have been previously reported to increase the delivery of agents to the brain [Kabanov et al. (1992) J. Controlled Release 22, 141-158]. In the present study, primary cultured bovine brain microvessel endothelial cells (BBMEC) were used as an in vitro model of the blood-brain barrier to examine ... More
N-type Ca2+ channels are located on somata, dendrites, and a subpopulation of dendritic spines on live hippocampal pyramidal neurons.
AuthorsMills LR, Niesen CE, So AP, Carlen PL, Spigelman I, Jones OT
JournalJ Neurosci
PubMed ID7525892
'In the nervous system the influx of Ca2+ orchestrates multiple biochemical and electrical events essential for development and function. A major route for Ca2+ entry is through voltage-dependent calcium channels (VDCCs). It is becoming increasingly clear that the precise contribution VDCCs make to neuronal function depends not only upon their ... More
Thalamic relay nuclei of the basal ganglia form both reciprocal and nonreciprocal cortical connections, linking multiple frontal cortical areas.
AuthorsMcFarland NR, Haber SN
JournalJ Neurosci
PubMed ID12223566
'Thalamic relay nuclei transmit basal ganglia output to the frontal cortex, forming the last link in corticobasal ganglia circuitry. The thalamus regulates cortical activity through differential laminar connections, providing not only feedback, but also initiating "feedforward" loops, via nonreciprocal projections, that influence higher cortical areas. This study examines the organization ... More
A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes.
'Gap junctions provide direct intercellular communication by linking adjacent cells with aqueous pores permeable to molecules up to 1 kDa in molecular mass and 8-14 A in diameter. The identification of over a dozen connexins in the mammalian gap junction family has stimulated interest in the functional significance of this ... More
Survey of the morphology of macaque retinal ganglion cells that project to the pretectum, superior colliculus, and parvicellular laminae of the lateral geniculate nucleus.
AuthorsRodieck RW, Watanabe M
JournalJ Comp Neurol
PubMed ID8308173
'In common with other vertebrates, the primate retina contains a number of different ganglion cell types that project to different regions in the brain. We wanted to determine how the different ganglion cell types, distinguished morphologically, mapped to these regions of the brain. We injected a fluorescent dye into one ... More
Electrophysiological and dye-coupling studies on secretory, myoepithelial and duct cells in human eccrine sweat glands.
AuthorsJones CJ, Kealey T
JournalJ Physiol
PubMed ID3681733
'1. Electrophysiological properties and dye-coupling status of secretory, myoepithelial and coiled duct cells in isolated human eccrine sweat glands have been assessed by single-micro-electrode studies and intracellular micro-iontophoresis of the fluorescent naphthalimide dye Lucifer Yellow CH (molecular weight 457). Treated glands were embedded in LKB HistoResin and examined by transmission ... More
The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications.
AuthorsSosinsky GE, Crum J, Jones YZ, Lanman J, Smarr B, Terada M, Martone ME, Deerinck TJ, Johnson JE, Ellisman MH,
JournalJ Struct Biol
PubMed ID17962040
'The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its own challenges for the preparation of thick sections. High pressure freezing in combination with freeze substitution provides the best method for obtaining the largest volume of well-preserved tissue. However, for ... More
A morphometric analysis of functionally defined subpopulations of neurons in the paraventricular nucleus of the rat with observations on the effects of colchicine.
AuthorsRho JH, Swanson LW
JournalJ Neurosci
PubMed ID2784832
'Two populations of neurons in the paraventricular nucleus of the hypothalamus that have different efferent projections and physiological roles in the regulation of visceral responses were characterized morphologically with a combined intracellular filling, retrograde tracer, and immunohistochemical method. Neuroendocrine cells were retrogradely labeled by an intravenous injection of Fast blue, ... More
Structure, function, and motility of vacuoles in filamentous fungi
AuthorsCole L, Orlovich DA, Ashford AE
JournalFungal Genet Biol
PubMed ID9742195
'Current information on the structure and function of motile tubular vacuoles in Pisolithus tinctorius and other fungi is reviewed. The use of fluorochromes to label the vacuole lumen is evaluated and observations on the structure and motility of vacuoles in P. tinctorius are differentiated from possible artifacts. The styryl dyes ... More
In vitro nasal transport across ovine mucosa: effects of ammonium glycyrrhizinate on electrical properties and permeability of growth hormone releasing peptide, mannitol, and lucifer yellow.
AuthorsReardon PM, Gochoco CH, Audus KL, Wilson G, Smith PL
JournalPharm Res
PubMed ID8483838
'Transport of growth hormone releasing peptide across ovine nasal mucosa in the absence or presence of ammonium glycyrrhizinate (AMGZ) was studied in vitro. Ovine nasal mucosa was stripped from underlying cartilage and mounted in Ussing chambers. Transepithelial conductance (Gt) and short-circuit current (Isc) were monitored during experiments to assess tissue ... More
Synchronized spontaneous Ca2+ transients in acute anterior pituitary slices.
AuthorsGuérineau NC, Bonnefont X, Stoeckel L, Mollard P
JournalJ Biol Chem
PubMed ID9553096
'We investigated the organization of spontaneous rises in cytosolic free Ca2+ concentration ([Ca2+]i) due to electrical activity in acute pituitary slices. Real time confocal imaging revealed that 73% of the cells generated fast peaking spontaneous [Ca2+]i transients. Strikingly, groups of apposing cells enhanced their [Ca2+]i in synchrony with a speed ... More
Intercellular communication in normal and aberrant crypts of rat colon mucosa.
AuthorsPassani MB, Luceri C, Caderni G, Dolara P
JournalCancer Lett
PubMed ID9461021
'An original system was developed to detect intercellular communication between epithelial cells of rat colon mucosa. Cell-to-cell communication was tested both in normal and in azoxymethane (AOM)-induced aberrant crypts in an attempt to identify chemically-induced modifications of cell properties. Stripes of unstained live tissue were superfused and oxygenated at room ... More
The relationship between gap-junctional intercellular communication and the proliferative activity of retinal pigment epithelial cells.
AuthorsYamori Y, Shiraki K, Moriwaki M, Miki T
JournalJpn J Ophthalmol
PubMed ID10913643
'PURPOSE: To investigate the relationship between gap-junction mediated intercellular communication and the proliferative activity of retinal pigment epithelial cells in the retinal tear base. METHODS Retinal tears were created experimentally in white rabbits, and the changes of intercellular communication via gap junctions between retinal pigment epithelial cells in the retinal ... More
Overexpression of a novel rho family GTPase, RacC, induces unusual actin-based structures and positively affects phagocytosis in Dictyostelium discoideum.
AuthorsSeastone DJ, Lee E, Bush J, Knecht D, Cardelli J
JournalMol Biol Cell
PubMed ID9763450
'Rho family proteins have been implicated in regulating various cellular processes, including actin cytoskeleton organization, endocytosis, cell cycle, and gene expression. In this study, we analyzed the function of a novel Dictyostelium discoideum Rho family protein (RacC). A cell line was generated that conditionally overexpressed wild-type RacC three- to fourfold ... More
Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast.
AuthorsPruyne DW, Schott DH, Bretscher A
JournalJ Cell Biol
PubMed ID9864365
'The actin cytoskeleton in budding yeast consists of cortical patches and cables, both of which polarize toward regions of cell growth. Tropomyosin localizes specifically to actin cables and not cortical patches. Upon shifting cells with conditionally defective tropomyosin to restrictive temperatures, actin cables disappear within 1 min and both the ... More
Dye tracers define differential endothelial and smooth muscle coupling patterns within the arteriolar wall.
AuthorsLittle TL, Xia J, Duling BR
JournalCirc Res
PubMed ID7859395
'Dye tracers were chosen, based on net charge, chemical structure, and reactive groups, to test for the existence of and to provide novel insight into channel selectivities of junctional pathways connecting smooth muscle and endothelial cells of the arteriolar wall. Dyes were injected into individual smooth muscle or endothelial cells ... More
ATP stimulation of P2X(7) receptors activates three different ionic conductances on cultured mouse Schwann cells.
AuthorsColomar A, Amédée T
JournalEur J Neurosci
PubMed ID11595031
'Extracellular ATP, by acting on P2 purinergic receptors, is a potent mediator of cell-to-cell communication both within and between the nervous and the immune systems. We show here by patch-clamp recording, fluorescent dye uptake and immunocytochemistry that, in cultured mouse Schwann cells, ATP activates a P2X(7) receptor associated with three ... More
Interaction of cholera toxin with ganglioside GM1 receptors in supported lipid monolayers.
AuthorsReed RA, Mattai J, Shipley GG
JournalBiochemistry
PubMed ID3567148
'Lipid monolayers formed at the air-water interface containing the ganglioside GM1 in egg yolk phosphatidylcholine have been transferred according to the Langmuir-Blodgett technique to glass cover slips coated with octadecyl- or hexadecyltrichlorosilane and carbon-coated electron microscope grids. Monolayer transfer has been demonstrated with fluorescence microscopy, by the transfer of a ... More
Synapse formation and morphological differentiation of neuron types in embryonic rat dentate gyrus explants in vitro.
AuthorsWerner M, Hatt H, Gottmann K
JournalBrain Res Dev Brain Res
PubMed ID9497075
'Cultured explants obtained from the dentate gyrus of rat embryos (embryonic day 19-20) were used to investigate synapse formation and morphological differentiation of neuron types in the absence of extrinsic afferents. Synaptogenesis was studied by whole-cell recordings of postsynaptic currents and by ultrastructural analysis. Neurons were visualized using Lucifer Yellow ... More
Permeance of Novikoff hepatoma gap junctions: quantitative video analysis of dye transfer.
AuthorsBiegon RP, Atkinson MM, Liu TF, Kam EY, Sheridan JD
JournalJ Membr Biol
PubMed ID3612766
'Fluorescent dyes are commonly used to study permeable (gap) junctions, but only rarely have quantitative values for junctional dye permeability been determined. In the present study, junctional permeance (PA, i.e., the product of the junctional permeability coefficient, P, times the junctional area, A) to Lucifer Yellow CH (LY) has been ... More
Weak acid-induced release of liposome-encapsulated carboxyfluorescein.
AuthorsBarbet J, Machy P, Truneh A, Leserman LD
JournalBiochim Biophys Acta
PubMed ID6326829
'Leakage of the entrapped anionic fluorophore carboxyfluorescein was used as a measure of the permeability of liposomes to several different acids. Carboxyfluorescein leakage increased with increasing buffer concentration at a given pH and depended on its chemical nature: apolar weak acids such as acetic or pyruvic acids induced fast leakage ... More
Free calcium in Micrasterias: local gradients are not detected in growing lobes.
AuthorsHolzinger A, Callaham DA, Hepler PK, Meindl U
JournalEur J Cell Biol
PubMed ID8521876
'Intracellular free Ca2+ ([Ca2+]) has been measured in growing unicells of two species of the green alga, Micrasterias, which have been injected with the indicator dye fura-2-dextran. Ratiometric imaging of Micrasterias denticulata yields levels of 170 to 200 nM [Ca2+] but fails to reveal a significant [Ca2+] gradient associated with ... More
Lipid-assisted microinjection: introducing material into the cytosol and membranes of small cells.
AuthorsLaffafian I, Hallett MB
JournalBiophys J
PubMed ID9788951
'The microinjection of synthetic molecules, proteins, and nucleic acids into the cytosol of living cells is a powerful technique in cell biology. However, the insertion of a glass micropipette into the cell is a potentially damaging event, which presents significant problems, especially for small mammalian cells (spherical diameter = 2-15 ... More
Dermal microvascular endothelial cells express the 180-kDa macrophage mannose receptor in situ and in vitro.
AuthorsGröger M, Holnthoner W, Maurer D, Lechleitner S, Wolff K, Mayr BB, Lubitz W, Petzelbauer P
JournalJ Immunol
PubMed ID11067894
'Expression of the 180-kDa mannose receptor (MR) is mainly found on cells of the macrophage lineage. MR mediates the uptake of micro-organisms and host-derived glycoproteins. We demonstrate that endothelium of the human skin in situ and dermal microvascular endothelial cells (DMEC) in vitro expressed MR at both the protein and ... More
In vivo visualization of living flatworm neurons using Lucifer yellow intracellular injections.
AuthorsKoopowitz H, Elvin M, Keenan L
JournalJ Neurosci Methods
PubMed ID8912938
'Turbellarian flatworms lend themselves to neurobiological investigations using intracellular iontophoresis of Lucifer yellow provided that one is able to anesthetize the animal and expose the nervous system. This paper details the methods used with the polyclad Notoplana acticola and the rhabdocoel Mesostoma ehrenbergii. Marine turbellarians can be anesthetized with equal ... More
Intracellular calcium during chemotaxis of Dictyostelium discoideum: a new fura-2 derivative avoids sequestration of the indicator and allows long-term calcium measurements.
AuthorsSchlatterer C, Knoll G, Malchow D
JournalEur J Cell Biol
PubMed ID1322818
'During stimulation of Dictyostelium discoideum amoebae with the chemoattractant cAMP, extracellular calcium is taken up by the cells. The aim of this study was to determine the cytosolic free calcium concentration ([Ca++]i) during chemotaxis of Dictyostelium cells. In contrast to most vertebrate cells, three major drawbacks were encountered: 1) the ... More
Concanavalin A: a tool to investigate neuronal plasticity.
AuthorsLin SS, Levitan IB
JournalTrends Neurosci
PubMed ID1719672
'Neuronal plasticity is the ability of neurons to alter their cellular properties in response to changes in their environment. These changes are typically triggered by the binding of specific ligands, such as neurotransmitters, growth factors or other neuromodulators, to receptors on the neuronal membrane surface. Since the extracellular domains of ... More
Electrophysiological properties of frog olfactory supporting cells.
AuthorsTrotier D
JournalChem Senses
PubMed ID9669050
'Cells, identified as supporting cells by Lucifer Yellow injection, were recorded from slices of frog olfactory epithelium using patch-clamp recordings. Cell-attached single-channel recordings indicated that the intracellular potential (IP) was -68 +/- 7 mV (n = 22) with 4 mM K+ in the bath ([K+]o). IP was -67 +/- 4 ... More
Mapping calcium transients in the dendrites of Purkinje cells from the guinea-pig cerebellum in vitro.
AuthorsRoss WN, Werman R
JournalJ Physiol
PubMed ID3681730
'1. A 10 X 10 photodiode array was used to detect stimulation-dependent absorbance changes simultaneously from many positions in the dendrite field of guinea-pig Purkinje cells which had been injected with the calcium indicator Arsenazo III in thin cerebellar slices. Signals from each element of the array were matched to ... More
Functional coupling in bovine ciliary epithelial cells is modulated by carbachol.
AuthorsStelling JW, Jacob TJ
JournalAm J Physiol
PubMed ID9435492
'The functional coupling of the ciliary epithelium was studied in isolated pairs (couplets) of pigmented ciliary epithelial (PCE) and nonpigmented ciliary epithelial (NPCE) cells using the whole cell patch clamp and the fluorescent dye lucifer yellow. One cell of the pair (usually the NPCE cell of a NPCE-PCE cell couplet) ... More