The release of oxygen radicals by blood and bone marrow leukocytes of patients with Fanconi anemia (FA) has been studied. It was found that the nonstimulated FA leukocytes and those stimulated by concanavalin A, SiO2, latex, and opsonized zymosan produced enhanced levels of luminol- and lucigenin-dependent chemiluminescence (CL) in comparison ... More
Role of oxidative stress and nitric oxide in regulation of spontaneous tone in aorta of DOCA-salt hypertensive rats.
AuthorsGhosh M, Wang HD, McNeill JR
JournalBr J Pharmacol
PubMed ID14744820
'1. The roles of nitric oxide (NO), superoxide anion (O(2)(-)), and hydrogen peroxide (H(2)O(2)) in the modulation of spontaneous tone were investigated in isolated aorta from deoxycorticosterone acetate (DOCA)-salt hypertensive rats. 2. Increases in preload from 1 to 5 g were accompanied by increases in spontaneous tone in aortic rings ... More
IL-3 induces down-regulation of CCR3 protein and mRNA in human eosinophils.
AuthorsDulkys Y, Kluthe C, Buschermöhle T, Barg I, Knöss S, Kapp A, Proudfoot AE, Elsner J
JournalJ Immunol
PubMed ID11544337
'Cytokines and chemokines are responsible for the attraction and activation of eosinophils in allergic and inflammatory diseases. Whereas cytokines such as IL-3, IL-5, and GM-CSF activate eosinophils via heterodimeric receptors containing a distinct alpha-chain (binding domain) and a common beta-chain (signaling domain), chemokines such as eotaxin activate eosinophils via seven-transmembrane ... More
Subcellular localization of Nox4 and regulation in diabetes.
AuthorsBlock K, Gorin Y, Abboud HE,
JournalProc Natl Acad Sci U S A
PubMed ID19706525
'Oxidative stress is implicated in human diseases. Some of the oxidative pathways are harbored in the mitochondria. NAD(P)H oxidases have been identified not only in phagocytic but also in somatic cells. Nox4 is the most ubiquitous of these oxidases and is a major source of reactive oxygen species (ROS) in ... More
Pleural surface fluorescence measurement of Na+ and Cl- transport across the air space-capillary barrier.
AuthorsJiang J, Song Y, Bai C, Koller BH, Matthay MA, Verkman AS
JournalJ Appl Physiol
PubMed ID12391048
'We developed a pleural surface fluorescence method to measure Na(+) and Cl(-) transport in perfused mouse lungs. The air space was filled with aqueous fluid containing membrane-impermeant fluorescent indicators of Cl(-) (lucigenin) or Na(+) (Sodium Green). After instillation of a Cl(-)-free solution into the air space, an increase in perfusate ... More
In vitro determination of phagocyte activity by luminol- and lucigenin-amplified chemiluminescence.
AuthorsMüller-Peddinghaus R
JournalInt J Immunopharmacol
PubMed ID6094369
'Amplified chemiluminescence (CL) detects most sensitively biologically important reactive oxygen species (ROS) which are generated by phagocytes by the respiratory burst permitting the determination of cell activity in vitro. Different murine phagocyte populations were used in combination with various ROS-catabolizing enzymes and some of their inhibitors to determine the possible ... More
Antioxidant activity of micronized diosmin on oxygen species from stimulated human neutrophils.
AuthorsCypriani B, Limasset B, Carrié ML, Le Doucen C, Roussie M, de Paulet AC, Damon M
JournalBiochem Pharmacol
PubMed ID8385947
'Flavonoids are known to reduce reactive oxygen species released by polymorphonuclear neutrophils (PMNs) in vitro. We have studied the effects of S5682 (Daflon 500 mg), a purified flavonoid fraction composed of 90% diosmin and 10% hesperidin. S5682 produced a dose-dependent inhibition of the luminol chemiluminescence (CL) induced by phorbol myristate ... More
Superoxide anion production by rabbit thoracic aorta: effect of endothelium-derived nitric oxide.
AuthorsPagano PJ, Tornheim K, Cohen RA
JournalAm J Physiol
PubMed ID7690193
'Rabbit thoracic aorta was assessed for the influence of the endothelium and nitric oxide (NO) on superoxide anion (SO) levels in the presence and absence of an inhibitor of superoxide dismutase. Aortic rings (0.5 cm) were incubated for 30 min at 37 degrees C in the presence or absence of ... More
'Indirect evidence suggests accelerated degradation of endothelium-derived nitric oxide (ENDO) by superoxide anion (O2-) in hypercholesterolemic vessels (HV). To directly measure O2- production by normal vessels (NV) and HV, we used an assay for O2- based on the chemiluminescence (CL) of lucigenin (L). HV (1 mo cholesterol-fed rabbits) produced threefold ... More
Thioridazine interacts with the membrane of mitochondria acquiring antioxidant activity toward apoptosis--potentially implicated mechanisms.
AuthorsRodrigues T, Santos AC, Pigoso AA, Mingatto FE, Uyemura SA, Curti C
JournalBr J Pharmacol
PubMed ID11976278
'We evaluated the effects of the phenothiazine derivative thioridazine on mechanisms of mitochondria potentially implicated in apoptosis, such as those involving reactive oxygen species (ROS) and cytochrome c release, as well as the involvement of drug interaction with mitochondrial membrane in these effects. Within the 0 - 100 microM range ... More
Effect of Polyoxidonium on the Phagocytic Activity of Human Peripheral Blood Leukocytes.
'The effect of polyoxidonium on the functional activity of human peripheral blood phagocytes was studied in vitro. Polyoxidonium is an N-oxidized polyethylene-piperazine derivative, a water-soluble high-molecular synthetic immunomodulator. It was established that a one-hour incubation of leukocytes with polyoxidonium increases the ability of leukocytes to kill the ingested Staphylococcus aureus ... More
Evidence for superoxide dismutase and catalase in mollicutes and release of reactive oxygen species.
AuthorsMeier B, Habermehl GG
JournalArch Biochem Biophys
PubMed ID2154957
'The presence of superoxide dismutase was demonstrated in 21 strains of mollicutes, including achuloplasmas, mycoplasmas, and ureaplasmas. No superoxide dismutase activities or only traces were detectable in fresh prepared cell lysates, whereas activities were evident after dialysis of the cell lysates. A further increase in superoxide dismutase activities was observed ... More
In vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor.
AuthorsSuematsu M, Kurose I, Asako H, Miura S, Tsuchiya M
JournalJ Biochem (Tokyo)
PubMed ID2808326
'Oxyradical-dependent chemiluminescence from granulocytes sticking to venular endothelium was successfully visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF). Intravital photonic image intensifier microscopy revealed that topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on endothelial walls and the subsequent activation of a luminol-dependent photonic burst. ... More
Increased NAD(P)H oxidase-mediated superoxide production in renovascular hypertension: evidence for an involvement of protein kinase C.
AuthorsHeitzer T, Wenzel U, Hink U, Krollner D, Skatchkov M, Stahl RA, MacHarzina R, Bräsen JH, Meinertz T, Münzel T
JournalKidney Int
PubMed ID9893134
'BACKGROUND: Angiotensin II infusion has been shown to cause hypertension and endothelial dysfunction and to increase superoxide (O-.2) production in vascular tissue, mainly via an activation of nicotinamide adenine dinucleotide (phosphate) [NAD(P)H]-dependent oxidase, the most significant O-.2 source in endothelial and/or smooth muscle cells. With these studies, we sought to ... More
Significance of glutathione depletion and oxidative stress in early embryogenesis in glucose-induced rat embryo culture.
AuthorsTrocino RA, Akazawa S, Ishibashi M, Matsumoto K, Matsuo H, Yamamoto H, Goto S, Urata Y, Kondo T, Nagataki S
JournalDiabetes
PubMed ID7622006
'Recent studies have demonstrated the protective effects of supplementing free oxygen radical scavenging enzymes against hyperglycemia-induced embryonic malformations. In this study, the glutathione (GSH)-dependent protection system in hyperglycemia-induced embryopathy was investigated. Rat embryos at the early head-fold stage (day 9.5) cultured in 66.7 mmol/l glucose for 48 h showed significant ... More
Oxygenation activities of chicken polymorphonuclear leukocytes investigated by selective chemiluminigenic probes.
AuthorsMerrill GA, Bretthauer R, Wright-Hicks J, Allen RC
JournalLab Anim Sci
PubMed ID8905586
'The redox metabolism of myeloperoxidase-deficient rooster (chicken) polymorphonuclear leukocytes (PMNL) was analyzed by differential chemiluminigenic probes. Chicken complement-opsonified zymosan, a phagocytosable particulate stimulus, and phorbol myristate acetate, a chemical stimulus, were used to activate the PMNL respiratory burst. The two probes used were luminol (5-amino-2,3-dihydro-1,4-phthalazinedione), a general probe of oxidase-peroxidase ... More
Interferon-gamma enhances monocyte cytotoxicity via enhanced reactive oxygen intermediate production. Absence of an effect on macrophage cytotoxicity is due to failure to enhance reactive nitrogen intermediate production.
AuthorsMartin JH, Edwards SW
JournalImmunology
PubMed ID8039809
'Interferon-gamma (IFN-gamma) enhanced the cytotoxic capability of freshly isolated human blood monocytes but failed to enhance the tumoricidal competence of monocyte-derived macrophages. Treatment of monocytes with IFN-gamma (100 U/ml) caused a significant increase (P < 0.001) in lucigenin-dependent chemiluminescence and O2- production stimulated by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) during the first few ... More
Fish oil treatment decreases superoxide anions in the myocardium and coronary arteries of atherosclerotic monkeys.
AuthorsSupari F, Ungerer T, Harrison DG, Williams JK
JournalCirculation
PubMed ID7850950
'BACKGROUND: This experiment sought to determine the effects of fish oil on superoxide anion production in the myocardium and coronary arteries of atherosclerotic monkeys. Recent evidence indicates that dietary supplementation with fish oil preserves normal vasomotion of atherosclerotic coronary arteries and reduces damage to the myocardium after ischemia and reperfusion, ... More
Detection of MCP-4 in dermal fibroblasts and its activation of the respiratory burst in human eosinophils.
'CC-chemokines are an important family of proinflammatory mediators that promote the recruitment and activation of human eosinophils in chronic inflammatory diseases. Recently, a novel human CC-chemokine, monocyte chemotactic protein 4 (MCP-4), has been reported that shows amino acid sequence similarities with eotaxin and RANTES, induces chemotaxis of eosinophils, and signals ... More
Pressure-induced conformational changes in a human Bence-Jones protein (Mcg).
AuthorsHerron JN, Ely KR, Edmundson AB
JournalBiochemistry
PubMed ID4041422
'The effect of high static pressures on the internal structure of the immunoglobulin light chain (Bence-Jones) dimer from the patient Mcg was assessed with measurements of intrinsic protein fluorescence polarization and intensity. Depolarization of intrinsic fluorescence was observed at relatively low pressures (less than 2 kbar), with a standard volume ... More
Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems.
AuthorsLi Y, Zhu H, Kuppusamy P, Roubaud V, Zweier JL, Trush MA
JournalJ Biol Chem
PubMed ID9442038
'Lucigenin is most noted for its wide use as a chemiluminescent detector of superoxide anion radical (O2-.) production by biological systems. However, its validity as a O2-.-detecting probe has recently been questioned in view of its ability to undergo redox cycling in several in vitro enzymatic systems, which produce little ... More
C-reactive protein selectively enhances the intracellular generation of reactive oxygen products by IgG-stimulated monocytes and neutrophils.
AuthorsZeller JM, Sullivan BL
JournalJ Leukoc Biol
PubMed ID1328445
'The acute phase protein, C-reactive protein (CRP), when heat-aggregated (Agg-CRP), potentiates immunoglobulin G (IgG) Fc receptor-mediated luminol-enhanced chemiluminescence (CL) in human monocytes and neutrophils. Luminol-CL is a sensitive measure of phagocyte respiratory burst activity; however, the nature of oxidative products contributing to the light emission and their site of generation ... More
Quantification of superoxide radical formation in intact vascular tissue using a Cypridina luciferin analog as an alternative to lucigenin.
AuthorsSkatchkov MP, Sperling D, Hink U, Anggard E, Münzel T
JournalBiochem Biophys Res Commun
PubMed ID9675145
'Lucigenin has been frequently used for the chemiluminescent detection of superoxide (*O-2) in intact tissue. More recent studies, however, revealed that lucigenin per se causes formation of *O-2 raising doubt about this probe to detect reliably *O-2. We therefore tested a more recently described chemiluminescence probe (2-methyl-6-phenyl-3,7-dihydroimidazol[1,2-alpha]pyrazine-3-one (CLA)) to estimate ... More
Further evidence that lucigenin-derived chemiluminescence monitors mitochondrial superoxide generation in rat alveolar macrophages.
AuthorsRembish SJ, Trush MA
JournalFree Radic Biol Med
PubMed ID7959172
'Lucigenin is well recognized for its ability to react with superoxide, yielding a product that emits chemiluminescence. Accordingly, lucigenin-derived chemiluminescence (LDCL) has been widely used to assess the generation of superoxide by the NADPH oxidase of leukocytes. Previously, we suggested that lucigenin could interact with mitochondrial-derived superoxide in alveolar macrophages. ... More
Effect of vitamin E on reoxygenation injury experienced by isolated rat hepatocytes.
AuthorsCaraceni P, Yao T, Degli Esposti S, Gasbarrini A, Bowie BT, Zern M, Borle AB, Van Thiel DH
JournalLife Sci
PubMed ID7934654
'The pathogenic role of lipid peroxidation in the reperfusion injury of the liver is still controversial. This study was performed to determine whether the damage caused by oxygen free radicals during reoxygenation in perfused rat hepatocytes is related to lipid peroxidation. Superoxide anion was detected by lucigenin-enhanced chemiluminescence. Lipid peroxidation ... More
Effect of linear polarized near-infrared ray irradiation on the chemiluminescence of human neutrophils and serum opsonic activity.
AuthorsShiraishi M, Suzuki K, Nakaji S, Sugawara K, Sugita N, Suzuki KJ, Ohta S
JournalLuminescence
PubMed ID10512987
'The purpose of this study was to investigate the in vitro effects of linear polarized near-infrared ray irradiation on neutrophil chemiluminescence (CL) and serum opsonic activity. We used luminol- and lucigenin-dependent CL to detect the affected reactive oxygen species production process of human neutrophils and measured serum opsonic activity based ... More
Reactive oxygen species associated with cell differentiation in Neurospora crassa.
AuthorsHansberg W, de Groot H, Sies H
JournalFree Radic Biol Med
PubMed ID8458586
'The conidiation process of Neurospora crassa is characterized by three morphogenetic events: adhesion of hyphae, development of aerial hyphae, and conidia formation. At the onset of all three events a spontaneous, low-level chemiluminescence was detected, indicating the formation of reactive oxygen species. Hyperoxic conditions increased chemiluminescence and accelerated differentiation. Hypoxic ... More
Use of a xanthine oxidase free radical generating system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa.
AuthorsAitken RJ, Buckingham D, Harkiss D
JournalJ Reprod Fertil
PubMed ID8388958
'The reaction between xanthine and xanthine oxidase results in the univalent and divalent reduction of dioxygen to generate superoxide (O2-.) and hydrogen peroxide (H2O2), respectively. With the aid of this system, the direct effect of reactive oxygen species (ROS) on human sperm function has been investigated. A protocol involving the ... More
Chemiluminescence in drug assay.
AuthorsCalokerinos AC, Deftereos NT, Baeyens WR
JournalJ Pharm Biomed Anal
PubMed ID8573629
'The phenomenon of chemiluminescence allows the development of simple analytical procedures using low cost instrumentation which can be easily controlled by computers. The sensitivity of the methods is excellent, and selectivity is improved by high-performance liquid chromatographic separation. A broad area of chemiluminometric applications is drug analysis, which is reviewed ... More
Endothelin-1 induces NAD(P)H oxidase in human endothelial cells.
AuthorsDuerrschmidt N, Wippich N, Goettsch W, Broemme HJ, Morawietz H
JournalBiochem Biophys Res Commun
PubMed ID10720482
'Superoxide anions (O(*-)(2)) induce oxidative stress and reduce endothelial NO availability by peroxynitrite formation. In human endothelial cells gp91(phox) was identified as the limiting subunit of the forming NAD(P)H oxidase. Because endothelin-1 (ET-1) is considered as a pro-atherosclerotic stimulus, we analyzed the effect of ET-1 on gp91(phox) expression and O(*-)(2) ... More
Anticonvulsive and free radical scavenging activities of vanillyl alcohol in ferric chloride-induced epileptic seizures in Sprague-Dawley rats.
AuthorsHsieh CL, Chang CH, Chiang SY, Li TC, Tang NY, Pon CZ, Hsieh CT, Lin JG
JournalLife Sci
PubMed ID10954052
'Vanillyl alcohol (VA) is a component of Gastrodia elata Bl. (GE), which is a traditional Chinese herb widely used to treat convulsive disorders or dizziness. This study examined the role of VA in the anticonvulsive properties of GE in a Sprague-Dawley rat model of epilepsy. The anticonvulsive and free radical ... More
Zonal distribution of the cation lucigenin in rat liver: influence of taurocholate.
AuthorsBraakman I, Verest O, Pijning T, Meijer DK, Groothuis GM
JournalMol Pharmacol
PubMed ID2811855
'The yellow fluorescent cation lucigenin (LU) was used as a model compound to study acinar heterogeneity in transport of hydrophilic cations that enter the liver by adsorptive endocytosis. Hepatic uptake was fast and saturable. The extraction was about 50% in a cyclically perfused rat liver preparation in which endogenous bile ... More
Lucigenin: redox potential in aqueous media and redox cycling with O-(2) production.
AuthorsSpasojevic I, Liochev SI, Fridovich I
JournalArch Biochem Biophys
PubMed ID10620371
'The use of lucigenin luminescence as a measure of ¿O-(2) has been questioned because lucigenin has been shown to be capable of mediating the production of O-(2). This being the case, lucigenin can signal the presence of O-(2) even in systems not producing it in the absence of lucigenin. The ... More
HSP25 overexpression attenuates oxidative stress-induced apoptosis: roles of ERK1/2 signaling and manganese superoxide dismutase.
AuthorsLee YJ, Cho HN, Jeoung DI, Soh JW, Cho CK, Bae S, Chung HY, Lee SJ, Lee YS
JournalFree Radic Biol Med
PubMed ID14975446
'HSP25 has been shown to induce resistance to radiation and oxidative stress; however, its exact mechanisms remain unclear. In the present study, a high concentration of H2O2 was found to induce DNA fragmentation in L929 mouse fibroblast cells, and HSP25 overexpression attenuated this phenomenon. To elucidate the mechanisms of H2O2-mediated ... More
Hyperoxia-induced NAD(P)H oxidase activation and regulation by MAP kinases in human lung endothelial cells.
AuthorsParinandi NL, Kleinberg MA, Usatyuk PV, Cummings RJ, Pennathur A, Cardounel AJ, Zweier JL, Garcia JG, Natarajan V
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12388366
'Hyperoxia increases reactive oxygen species (ROS) production in vascular endothelium; however, the mechanisms involved in ROS generation are not well characterized. We determined the role and regulation of NAD(P)H oxidase in hyperoxia-induced ROS formation in human pulmonary artery endothelial cells (HPAECs). Exposure of HPAECs to hyperoxia for 1, 3, and ... More
A critical investigation of NADPH oxidase activity in human spermatozoa.
AuthorsRicher SC, Ford WC
JournalMol Hum Reprod
PubMed ID11228243
'It has been suggested that human spermatozoa contain an NADPH oxidase that could generate reactive oxygen species involved in signalling pathways to promote fertility. The proposal depends on observations that the addition of NADPH to purified human spermatozoa stimulates chemiluminescence by the superoxide (O2-) probe, lucigenin. We confirmed these observations, ... More
Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012.
AuthorsDaiber A, August M, Baldus S, Wendt M, Oelze M, Sydow K, Kleschyov AL, Munzel T
JournalFree Radic Biol Med
PubMed ID14732294
'In the present study we sought to determine the ability of the chemiluminescence dye 8-amino-5-chloro-7-phenylpyridol[3,4-d]pyridazine-1,4-(2H,3H)dione sodium salt (L-012) to detect superoxide in different biological systems. In human whole blood or isolated leukocytes, the sensitivity of the luminol analogue L-012 to detect superoxide was higher as compared with luminol, lucigenin, coelenterazine, ... More
Vascular oxidant stress early after balloon injury: evidence for increased NAD(P)H oxidoreductase activity.
AuthorsSouza HP, Souza LC, Anastacio VM, Pereira AC, Junqueira ML, Krieger JE, da Luz PL, Augusto O, Laurindo FR
JournalFree Radic Biol Med
PubMed ID10889453
'Available evidence for oxidative stress after angioplasty is indirect or ambiguous. We sought to characterize the pattern, time course, and possible sources of free radical generation early after arterial balloon injury. Ex vivo injury performed in arterial rings in buffer with lucigenin yielded a massive oxygen-dependent peak of luminescence that ... More
Agonist-induced peroxynitrite production from endothelial cells.
AuthorsKooy NW, Royall JA
JournalArch Biochem Biophys
PubMed ID8179319
'Nitric oxide reacts with superoxide to form peroxynitrite, a potential mediator of oxidant-induced cellular injury. The endothelium is a primary target of injury in many pathological states, including acute lung injury, sepsis, multiple organ failure syndrome, and atherosclerosis, where enhanced production of nitric oxide and superoxide occurs simultaneously. It was ... More
Endogenous production of superoxide by rabbit lungs: effects of hypoxia or metabolic inhibitors.
AuthorsPaky A, Michael JR, Burke-Wolin TM, Wolin MS, Gurtner GH
JournalJ Appl Physiol
PubMed ID8396109
'We find spontaneous light emission from isolated Krebs-Henseleit-perfused rabbit lungs when the light-emitting super-oxide trap lucigenin is added to the perfusate. Lucigenin light emission appears to be specific for superoxide anion, because light emission from the lung caused by a superoxide-generating system is abolished by superoxide dismutase but not by ... More
A novel long chain polyunsaturated fatty acid, beta-Oxa 21:3n-3, inhibits T lymphocyte proliferation, cytokine production, delayed-type hypersensitivity, and carrageenan-induced paw reaction and selectively targets intracellular signals.
AuthorsCostabile M, Hii CS, Robinson BS, Rathjen DA, Pitt M, Easton C, Miller RC, Poulos A, Murray AW, Ferrante A
JournalJ Immunol
PubMed ID11564817
'A novel polyunsaturated fatty acid (PUFA), beta-oxa 21:3n-3, containing an oxygen atom in the beta position, was chemically synthesized, and found to have more selective biological activity than the n-3 PUFA, docosahexaenoic acid (22:6n-3) on cells of the immune system. Although beta-oxa 21:3n-3 was very poor compared with 22:6n-3 at ... More
A new highly sensitive chemiluminescent assay of alkaline phosphatase using lucigenin and its application to enzyme immunoassay.
AuthorsKitamura M, Maeda M, Tsuji A
JournalJ Biolumin Chemilumin
PubMed ID7762411
'The chemiluminescent reaction of lucigenin with various reducing sugars and reducing compounds has been studied. It was found that dihydroxyacetone gave the most intense chemiluminescence (CL). We have developed highly sensitive chemiluminescent methods for alkaline phosphatase (ALP) based on the production of dihydroxyacetone using NADP+ or glycerol-3-phosphate as substrate. The ... More
Detergent-amplified chemiluminescence of lucigenin for determination of superoxide anion production by NADPH oxidase and xanthine oxidase.
AuthorsStorch J, Ferber E
JournalAnal Biochem
PubMed ID2837920
'The detergent-induced amplification of lucigenin-dependent chemiluminescence of O2-, generated by xanthine oxidase or microsomal NADPH oxidase was studied. An assay system is described which is at least 10 times more sensitive than normal lucigenin-dependent chemiluminescence due to the amplification by high concentrations of octylphenylpolyethylene glycol (Triton X-100). Compared to the ... More
Chemiluminescence detection for high-performance liquid chromatography of biomedical samples.
AuthorsDe Jong GJ, Kwakman PJ
JournalJ Chromatogr
PubMed ID2670996
'During recent years, much progress has been made in the development of high-performance liquid chromatographic (HPLC) detection systems based on chemiluminescence (CL). CL is now one of the most sensitive detection methods in HPLC. For many compounds detection limits in the femtogram to picogram range have been obtained. Several on-line ... More
Hydroxyl radical generation: the effect of bicarbonate, dioxygen and buffer concentration on pH-dependent chemiluminescence.
AuthorsOosthuizen MM, Greyling D
JournalRedox Rep
PubMed ID11450981
'Enhanced chemiluminescence (CL) induced by HO* was detected using the primary enhancers luminol (Lum), isoluminol (ILum) and lucigenin (Luc), in the absence of HCO3-, at pH 9.5, 10.0 and 10.5 but not between pH 4.0 and 9.0. This was confirmed using nine different HO* generators. FeCl3/NTA/H2O2 was the only HO* ... More
Lucigenin chemiluminescence as a probe for measuring reactive oxygen species production in Escherichia coli.
AuthorsPeters TR, Tosk JM, Goulbourne EA
JournalAnal Biochem
PubMed ID2194399
'Addition of oxygen to whole cells of Escherichia coli suspended in the presence of the chemiluminescent probe bis-N-methylacridinium nitrate (lucigenin) resulted in a light emission increase of 200% of control. Addition of air to cells showed a chemiluminescent response far less than the response to oxygen. The redox cycling agents ... More
Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice.
AuthorsMeloni-Bruneri LH, Campa A, Abdalla DS, Calich VL, Lenzi HL, Burger E
JournalJ Leukoc Biol
PubMed ID8613700
'The oxidative burst of polymorphonuclear neutrophils (PMN) and their ability to inhibit Paracoccidioides brasiliensis growth was studied in susceptible (B10.A) and resistant (A/J) mice. The cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure PMN preparations; the number of cells was similar for both strains at 24 ... More
Micelle-enhanced chemiluminescence and application to the determination of biological reductants using lucigenin.
AuthorsHinze WL, Riehl TE, Singh HN, Baba Y
JournalAnal Chem
PubMed ID6507863
Luminescent bioassays based on macrophage cell lines.
AuthorsDe Baetselier P, Schram E
JournalMethods Enzymol
PubMed ID3547032
Phagocytic leukocyte oxygenation activities and chemiluminescence: a kinetic approach to analysis.
AuthorsAllen RC
JournalMethods Enzymol
PubMed ID3821549
Fluorescence and chemiluminescence determination of steroid and bile acid sulphates with lucigenin by flow injection analysis based on ion-pair solvent extraction.
AuthorsMaeda M, Tsuji A
JournalAnalyst
PubMed ID4037353
Development of chemiluminescence reactions in biomedical analysis.
AuthorsBaeyens WR, Nakashima K, Imai K, Ling BL, Tsukamoto Y
JournalJ Pharm Biomed Anal
PubMed ID2490747
Determination of conjugated glucuronic acid by combining enzymatic hydrolysis with lucigenin chemiluminescence.
AuthorsKlopf LL, Nieman TA
JournalAnal Chem
PubMed ID3985340
Pattern of formylmethionyl-leucyl-phenylalanine-induced luminol- and lucigenin-dependent chemiluminescence in human neutrophils.
AuthorsDahlgren C, Aniansson H, Magnusson KE
JournalInfect Immun
PubMed ID3965405
The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only ... More
Luminol and lucigenin as detectors for O2.-.
AuthorsFaulkner K, Fridovich I
JournalFree Radic Biol Med
PubMed ID8225026
Univalent oxidation of luminol and univalent reduction of lucigenin must precede reaction with O2.- if that reaction is to lead to luminescence. The assumption that luminol or lucigenin, per se, reacts with O2.- in a way leading to luminescence is incorrect, and leads to misinterpretation of results. The chemical reactions ... More
A new technique for enhancing luminol luminescent detection of free radicals and reactive oxygen species.
AuthorsTrevithick JR, Dzialoszynski T
JournalBiochem Mol Biol Int
PubMed ID7804144
A novel technique is reported which makes use of (1) an improved method for solubilizing luminol at neutral pH along with (2) the addition of dimethyl sulfoxide to stabilize superoxide anion in the solutions in which luminescence is detected. These improvements resulted in (1) very low blank values of luminescence ... More
Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations.
AuthorsTarpey MM, Wink DA, Grisham MB
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID14761864
Facile detection of reactive oxygen and nitrogen species in biologic systems is often problematic. This is a result of the numerous cellular mechanisms, both enzymatic and nonenzymatic involved in their catabolism/decomposition, the complex and overlapping nature of their reactivities, as well as the often limited intracellular access of detector systems. ... More
Effect of salmeterol on polymorphonuclear leukocyte (PMNL) chemiluminescence in vitro.
AuthorsRamage L, Blair AL, Cree IA, Dhillon DP
JournalJ Biolumin Chemilumin
PubMed ID7901968
Although beta-agonists remain an important aspect of the treatment of asthma, their role has recently been questioned. Salmeterol has recently been developed as a beta-agonist with prolonged bronchodilator action. Using lucigenin-enhanced chemiluminescence, we have shown that salmeterol inhibits this aspect of phagocyte function in vitro in a concentration-dependent manner. However, ... More
A new technique for highly sensitive detection of superoxide dismutase activity by chemiluminescence.
AuthorsCorbisier P, Houbion A, Remacle J
JournalAnal Biochem
PubMed ID2823632
A stable enzymatic free radical generation system has been developed which allows a precise production of 02-. and its detection by chemiluminescence between 2 pmol and 8 nmol. This test has been used for assaying superoxide dismutase (SOD) by inhibition of the chemiluminescence (CL) signal. No inhibition was observed with ... More
Enzymatic quantification of cell-matrix and cell-cell adhesion.
AuthorsLöster K, Horstkorte R
JournalMicron
PubMed ID10568230
Adhesion assays are powerful tools to investigate the adhesive properties of cells. The quantification of cell adhesion enables determination of the capacity of cells to stick to a target, screening for novel adhesion involved binding molecules, exploration of structure-function relationships of adhesion molecules, evaluation of adhesion targets, and examination of ... More
Potential role of the peroxidase-dependent metabolism of serotonin in lowering the polymorphonuclear leukocyte bactericidal function.
AuthorsSalman-Tabcheh S, Guérin MC, Torreilles J
JournalFree Radic Res
PubMed ID8747893
Serotonin (5-hydroxytryptamine, 5-HT) significantly and dose-dependently suppressed the luminol-enhanced chemiluminescence (CL) signal generated by polymorphonuclear leukocytes (PMN) activated with phorbol myristate acetate (PMA), but did not modify either lucigenin-enhanced CL or the reduction of superoxide dismutase-inhibitable cytochrome c. Moreover, stimulation of PMNs previously incubated with 5-HT resulted in a threefold ... More
Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes.
AuthorsKervinen M, Pätsi J, Finel M, Hassinen IE
JournalAnal Biochem
PubMed ID14654044
The chemiluminescent superoxide indicators lucigenin and coelenterazine were compared in rat liver submitochondrial particles and cytoplasmic membranes from Paracoccus denitrificans. Qualitative monitoring is possible with both probes, but quantitative work with lucigenin is hampered by its dependence on one-electron reduction before the photon-emitting reaction. Therefore, calibration of measurements on complex ... More
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
AuthorsWardman P,
JournalFree Radic Biol Med
PubMed ID17761297
Chemical probes for free radicals in biology are important tools; fluorescence and chemiluminescence offer high detection sensitivity. This article reviews progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use. Reactive species include hydrogen peroxide; hydroxyl, superoxide, and thiyl radicals; carbonate ... More
Superoxide production by normal and chronic granulomatous disease (CGD) patient-derived EBV-transformed B cell lines measured by chemiluminescence-based assays.
AuthorsPorter CD, Parkar MH, Collins MK, Levinsky RJ, Kinnon C
JournalJ Immunol Methods
PubMed ID1331241
We have compared assays for products of the neutrophil respiratory burst in normal EBV-transformed B cell lines stimulated with agonists of protein kinase C. Those measuring O2- directly or its immediate product, H2O2, were successful. Of these, the most sensitive were the lucigenin- and luminol-based chemiluminescence assays for O2- and ... More
Mitochondrial reactive oxygen species activate the slow force response to stretch in feline myocardium.
AuthorsCaldiz CI, Garciarena CD, Dulce RA, Novaretto LP, Yeves AM, Ennis IL, Cingolani HE, Chiappe de Cingolani G, Pérez NG,
JournalJ Physiol
PubMed ID17823205
When the length of the myocardium is increased, a biphasic response to stretch occurs involving an initial rapid increase in force followed by a delayed slow increase called the slow force response (SFR). Confirming previous findings involving angiotensin II in the SFR, it was blunted by AT1 receptor blockade (losartan). ... More
Spectral studies of the binding of lucigenin, a bisacridinium derivative, with double-helix DNA.
AuthorsWu HL, Li WY, He XW, Miao K, Liang H
JournalAnal Bioanal Chem
PubMed ID12043019
The non-covalent binding of the cationic reagent lucigenin to DNA has been investigated by spectroscopic methods. Results from absorption, circular dichroism, and fluorescence studies revealed that lucigenin could intercalate into the helix of DNA. Polarization and melting studies provided further evidence of the intercalation binding of lucigenin with DNA. The ... More
Effect of vitamin B6 on oxygen radicals, mitochondrial membrane potential, and lipid peroxidation in H2O2-treated U937 monocytes.
AuthorsKannan K, Jain SK
JournalFree Radic Biol Med
PubMed ID14975445
Vitamin B6 (Vit.B6) supplementation has been shown to be beneficial in reducing diabetic complications, cognitive aging, and in the prevention of coronary heart disease. It was hypothesized that Vit.B6 compounds may function as antioxidants and thus offer protection against oxidative stress under various pathophysiological and or experimental conditions. To test ... More
Dual lifetime referencing as applied to a chloride optical sensor.
AuthorsHuber C, Klimant I, Krause C, Wolfbeis OS
JournalAnal Chem
PubMed ID11354496
A membrane with an optical response to chloride has been developed that contains two luminophores that display two largely different decay times. The first luminophore (the "reference") is a chloride-insensitive ruthenium metal-ligand complex possessing a decay time in the microsecond range. The second luminophore is the short-lived chloride-quenchable fluorescent probe ... More
Lucigenin-dependent chemiluminescence as a new assay for NAD(P)H-oxidase activity in particulate fractions of human polymorphonuclear leukocytes.
AuthorsMinkenberg I, Ferber E
JournalJ Immunol Methods
PubMed ID6725961
The enzyme responsible for the respiratory burst in human neutrophils is an oxidase that catalyzes the reduction of oxygen to superoxide anion (O-2). Superoxide anion production may be measured by chemiluminescence (CL) in the presence of lucigenin (10,10'-dimethyl-9,9'- biacridinium dinitrate). We established an assay of the oxidase, by measuring the ... More
Interaction of chlormezanone enantiomers with rat liver microsomes.
AuthorsKlinger W, Oelschläger H, Rothley D, Karge E, Seeling A
JournalEur J Drug Metab Pharmacokinet
PubMed ID10412893
Both chlormezanone enantiomers, for the first time obtained by enantiospecific HPLC with a 100% yield, bind to oxidized cytochrome P-450 in rat liver microsomes with a binding curve according to type I, similar to hexobarbital but less pronounced. There are no differences between the binding curves of the two enantiomers. ... More
Lucigenin and linoleate enhanced chemiluminescent assay for superoxide dismutase activity.
AuthorsLaihia JK, Jansén CT, Ahotupa M
JournalFree Radic Biol Med
PubMed ID8394267
The xanthine/xanthine oxidase dependent chemiluminescence was enhanced both by lucigenin and linoleate to create a sensitive, specific, and rapid chemiluminescent method for superoxide dismutase (SOD) activity determination. A pH optimum at around 10.0 was found both for the chemiluminescence and its inhibition by SOD. At this pH, a linear inhibition ... More
Characterization of murine lung interstitial macrophages in comparison with alveolar macrophages in vitro.
AuthorsFranke-Ullmann G, Pförtner C, Walter P, Steinmüller C, Lohmann-Matthes ML, Kobzik L
JournalJ Immunol
PubMed ID8816420
The present study was performed to characterize the immunologic potential of interstitial macrophages (INT) in comparison with alveolar macrophages (AL). The data showed that AL, compared with INT, have a more efficient phagocytic potential. In addition, they have a strong microbicidal activity and secrete large amounts of reactive oxygen radicals, ... More
IgM monoclonal antibodies recognizing Fc alpha R but not Fc gamma RIII trigger a respiratory burst in neutrophils although both trigger an increase in intracellular calcium levels and degranulation.
AuthorsMackenzie SJ, Kerr MA
JournalBiochem J
PubMed ID7887905
The role of IgG receptor, Fc gamma RIII, in the triggering of neutrophil functions has been controversial. Here we show that IgM monoclonal antibodies, Leu 11b and 1D3, recognizing Fc gamma RIII, bind to human neutrophils triggering an increase in intracellular calcium concentration and release of myeloperoxidase upon degranulation but ... More
Lucigenin luminescence as a measure of intracellular superoxide dismutase activity in Escherichia coli.
AuthorsLiochev SI, Fridovich I
JournalProc Natl Acad Sci U S A
PubMed ID9096317
Lucigenin and paraquat are similar in that each can be taken into Escherichia coli and can then mediate O2.- production by cycles of univalent reduction, to the corresponding monocation radical, followed by autoxidation. Thus, both compounds caused induction of enzymes that are regulated by the soxRS regulon. The lucigenin cation ... More
Imaging reactive oxygen species in asthma.
AuthorsVachier I, Le Doucen C, Loubatière J, Damon M, Térouanne B, Nicolas JC, Chanez P, Godard P
JournalJ Biolumin Chemilumin
PubMed ID7942122
Inflammatory processes in asthma are characterized by an infiltration of inflammatory cells including mononuclear phagocytes. It has been observed that mononuclear phagocytes, alveolar macrophages and blood monocytes, release higher quantities of reactive oxygen species in asthmatic patients than in healthy subjects. Chemiluminescence assays were developed to measure the superoxide anion ... More
Analysis of nanoliter samples of electrolytes using a flow-through microfluorometer.
AuthorsZhelyaskov VR, Liu S, Broderick MP
JournalKidney Int
PubMed ID10760113
Several techniques have been developed to study the transport properties of nanoliter samples of renal tubule segments, such as continuous flow colorimetry and continuous fluorometry. We have extended the capability of the NANOFLO, a flow-through microfluorometer, designed for measurement of carbon dioxide, urea, ammonia, glucose, lactate, etc., to analyze sodium, ... More
Lucigenin chemiluminescence in the assessment of neutrophil superoxide production.
AuthorsGyllenhammar H
JournalJ Immunol Methods
PubMed ID3029229
Lucigenin-amplified chemiluminescence (LUCL) was induced by .O2- (from the xanthine/xanthine-oxidase reaction) but not, as with luminol-augmented CL (LCL), by myeloperoxidase (MPO) and only weakly by H2O2 or the H2O2-MPO-Cl- system. Neutrophil LUCL induced by fMet-Leu-Phe (fMLP) was dose-dependently inhibited by scavengers of .O2- but not by NaN3, catalase, mannitol or ... More
Changes in mechanisms of monocyte/macrophage-mediated cytotoxicity during culture. Reactive oxygen intermediates are involved in monocyte-mediated cytotoxicity, whereas reactive nitrogen intermediates are employed by macrophages in tumor cell killing.
AuthorsMartin JH, Edwards SW
JournalJ Immunol
PubMed ID8385686
Freshly isolated human blood monocytes were spontaneously cytotoxic toward K562 tumor cells. During culture of the monocytes in vitro cytotoxicity decreased during the first 48 h but tumoricidal competence was restored after 3 to 4 days in vitro. These changes were accompanied by changes in both reactive oxygen intermediate generating ... More
Vascular release of superoxide radicals is enhanced in hypercholesterolemic rabbits.
AuthorsMügge A, Brandes RP, Böger RH, Dwenger A, Bode-Böger S, Kienke S, Frölich JC, Lichtlen PR
JournalJ Cardiovasc Pharmacol
PubMed ID7898085
Production/release of superoxide anions from aortic rings was measured by a modified lucigenin-enhanced chemiluminescence (CL) technique. The aortic rings were obtained from control and cholesterol-fed (1% for 12 weeks) rabbits. The CL signal was significantly increased in aortic wall of cholesterol-fed rabbits. Pretreatment with oxypurinol, an inhibitor of xanthine oxidase, ... More
Is superoxide an initiator of microsomal lipid peroxidation?
The effects of "pro-oxidant" quinones, doxorubicin, Fe(3+)-ADP-doxorubicin complex, and menadione, as well as of free radical scavengers possessing superoxide-dismuting activities, Fe(3+)-rutin and Cu(2+)-rutin, on superoxide production and lipid peroxidation in rat liver microsomes have been studied. All quinone compounds efficiently suppressed lucigenin-dependent chemiluminescence produced in NADPH-dependent microsomal lipid peroxidation, but ... More
Activated or dominant inhibitory mutants of Rap1A decrease the oxidative burst of Epstein-Barr virus-transformed human B lymphocytes.
AuthorsMaly FE, Quilliam LA, Dorseuil O, Der CJ, Bokoch GM
JournalJ Biol Chem
PubMed ID8034626
Rap1A is a GTP-binding protein of the Ras superfamily that is highly abundant in phagocyte membranes. Although Rap1A copurifies with cytochrome b558, a component of the superoxide-generating NADPH oxidase complex of human phagocytes and B lymphocytes, the involvement of Rap1A in the regulation of the oxidative burst in these cells ... More
The goal of this study was to develop long-wavelength fluorescent Cl indicators that have improved optical properties over quinolinium compounds. A series of quaternized tricyclic heterocycles was screened. We found that N,N-dimethyl-9,9-bisacridinium (lucigenin) had very high halide sensitivity with Stern-Volmer constants for collisional quenching of 390 M-1 (Cl), 585 M-1 ... More
Sulfite stimulates NADPH oxidase of human neutrophils to produce active oxygen radicals via protein kinase C and Ca2+/calmodulin pathways.
AuthorsBeck-Speier I, Liese JG, Belohradsky BH, Godleski JJ
JournalFree Radic Biol Med
PubMed ID8392022
The effect of sulfite on the oxidative metabolism of human neutrophils was studied in vitro. Superoxide anion production of PMN was determined using superoxide dismutase-inhibitable lucigenin-dependent CL. The addition of sulfite in concentrations of 0.01 mM-1 mM results in an up to 6-fold increase in CL of nonstimulated neutrophils at ... More
A mechanism for tamoxifen-mediated inhibition of acidification.
AuthorsChen Y, Schindler M, Simon SM
JournalJ Biol Chem
PubMed ID10373441
Tamoxifen has been reported to inhibit acidification of cytoplasmic organelles in mammalian cells. Here, the mechanism of this inhibition is investigated using in vitro assays on isolated organelles and liposomes. Tamoxifen inhibited ATP-dependent acidification in organelles from a variety of sources, including isolated microsomes from mammalian cells, vacuoles from Saccharomyces ... More
Reactive oxygen species are involved in the apoptosis induced by nonsteroidal anti-inflammatory drugs in cultured gastric cells.
AuthorsKusuhara H, Komatsu H, Sumichika H, Sugahara K
JournalEur J Pharmacol
PubMed ID10594327
We previously reported the induction of apoptotic DNA fragmentation by nonsteroidal anti-inflammatory drugs (NSAIDs) in cultured rat gastric cells, and indicated that prostaglandin-synthesis is only marginally involved in the apoptotic process. In the present study, we examined whether the generation of reactive oxygen species is critically involved in NSAID-induced apoptosis. ... More
NADH oxidoreductase is a major source of superoxide anion in bovine coronary artery endothelium.
AuthorsMohazzab KM, Kaminski PM, Wolin MS
JournalAm J Physiol
PubMed ID8024019
In this study we examined the intracellular sources of superoxide anion (O2-.) in cultured bovine coronary endothelium, employing lucigenin (250 microM)-elicited chemiluminescence (CL). In the homogenate from these cells, 100 microM NADPH increased O2-. by 81% from 8.9 +/- 1.5 to 16.0 +/- 1.5 x 10(5) cpm/mg protein (P < ... More
Vesicular uptake system for the cation lucigenin in the rat hepatocyte.
AuthorsBraakman I, Pijning T, Verest O, Weert B, Meijer DK, Groothuis GM
JournalMol Pharmacol
PubMed ID2811856
The hepatic transport mechanism for the fluorescent bivalent hydrophilic organic cation lucigenin (LU) was characterized employing kinetic and morphological methods. The extraction of LU by the perfused rat liver was 50% and uptake was saturable. LU did not inhibit the carrier-mediated hepatic uptake of the model organic cationic compounds tributylmethyl ... More
Oxidative stress in human abdominal aortic aneurysms: a potential mediator of aneurysmal remodeling.
Abdominal aortic aneurysm (AAA) is an inflammatory disorder characterized by localized connective tissue degradation and smooth muscle cell (SMC) apoptosis, leading to aortic dilatation and rupture. Reactive oxygen species are abundantly produced during inflammatory processes and can stimulate connective tissue-degrading proteases and apoptosis of SMCs. We hypothesized that reactive oxygen ... More
The fatty acid bimodal action on superoxide anion production by human adherent monocytes under phorbol 12-myristate 13-acetate or diacylglycerol activation can be explained by the modulation of protein kinase C and p47phox translocation.
AuthorsKadri-Hassani N, Léger CL, Descomps B
JournalJ Biol Chem
PubMed ID7797495
We studied the translocation of protein kinase C (PKC), the endogenous phosphorylation and presence in the membrane fraction of p47phox (the 47-kDa cytosolic component of the phagocyte NADPH oxidase), and the O-.2 production in human adherent monocytes (HAMs). This was performed under phorbol myristate acetate (PMA) or diacylglycerol stimulation after ... More
Tumour necrosis factor-alpha induces superoxide anion generation in mitochondria of L929 cells.
AuthorsHennet T, Richter C, Peterhans E
JournalBiochem J
PubMed ID7678739
Within a few minutes after addition to L929 cells, tumour necrosis factor-alpha (TNF alpha) induced an increase in lucigenin-enhanced chemiluminescence that could be inhibited by superoxide dismutase. The generation of superoxide anion (O2.-) was sensitive to treatment with rotenone, antimycin A and cyanide, indicating that the signal originated from mitochondria. ... More
The effect of pH on chemiluminescence of different probes exposed to superoxide and singlet oxygen generators.
AuthorsOosthuizen MM, Engelbrecht ME, Lambrechts H, Greyling D, Levy RD
JournalJ Biolumin Chemilumin
PubMed ID9509335
The compromised optima for high intensity chemiluminescence (CL), using superoxide generators, were all above pH 9.0 for the CL probes luminol and lucigenin. With luminol the optima were at pH 9.0 and 9.4 for the generators KO2 and hypoxanthine/xanthine oxidase (HX/XO), respectively. Lucigenin, with the same generators, produced optima at ... More
Uncoupling-mediated generation of reactive oxygen by halogenated aromatic hydrocarbons in mouse liver microsomes.
Studying liver microsomes from 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced or vehicle-treated (noninduced) mice, we evaluated the in vitro effects of added chemicals on the production of reactive oxygen due to substrate/P450-mediated uncoupling. The catalase-inhibited NADPH-dependent H(2)O(2) production (luminol assay) was lower in induced than noninduced microsomes. The effects of adding chemicals (2.5 microM) ... More
Chemiluminescence of mononuclear cells is enhanced during antigen recognition.
AuthorsTengler RS, Furukawa K, de Weck AL, Maly FE
JournalJ Biolumin Chemilumin
PubMed ID8493886
Stimulation of phagocytes by several cytokines causes superoxide generation and consequently chemiluminescence. Since antigen-activated lymphocytes generate cytokines, we investigated whether antigen recognition by mononuclear cells, which contain both lymphocytes and monocytes, is accompanied by changes in lucigenin-dependent chemiluminescence. Mononuclear cells which underwent antigen-induced proliferation showed a delayed rise in lucigenin-dependent ... More
Suppression of the reactive oxygen intermediates production of human macrophages by colorectal adenocarcinoma cell lines.
AuthorsSiegert A, Denkert C, Leclere A, Hauptmann S
JournalImmunology
PubMed ID10594687
Although some in vitro studies indicate that macrophages exert cytotoxic responses against tumour cells by production of reactive oxygen intermediates (ROI), no obvious impairment of tumour cell growth is visible in various human malignant tumours, which contain a large number of tumour-associated macrophages (TAM). We made use of an in ... More
A metabolic enzyme that rapidly produces superoxide, fumarate reductase of Escherichia coli.
AuthorsImlay JA
JournalJ Biol Chem
PubMed ID7649986
Aerobic organisms synthesize superoxide dismutases in order to escape injury from endogenous superoxide. An earlier study of Escherichia coli indicated that intracellular superoxide is formed primarily by autoxidation of components of the respiratory chain. In order to identify those components, inverted respiratory vesicles were incubated with five respiratory substrates. In ... More
Superoxide formed from cigarette smoke impairs polymorphonuclear leukocyte active oxygen generation activity.
AuthorsTsuchiya M, Thompson DF, Suzuki YJ, Cross CE, Packer L
JournalArch Biochem Biophys
PubMed ID1332616
Reactive free radicals contained in cigarette smoke (CS) and compromised phagocytic antimicrobial activities including those of polymorphonuclear leukocytes (PMNs) have been implicated in the pathogenesis of severe CS-related pulmonary disorders. In CS-exposed buffer solutions, O2-. was the predominant generated reactive oxygen species, as demonstrated by lucigenin-amplified chemiluminescence and electron spin ... More
Angiotensin II-induced vascular dysfunction is mediated by the AT1A receptor in mice.
AuthorsRyan MJ, Didion SP, Mathur S, Faraci FM, Sigmund CD
JournalHypertension
PubMed ID15007032
Many of the actions of angiotensin II (Ang II) are mediated by angiotensin type 1 receptors (AT1), of which there are 2 pharmacologically indistinguishable subtypes (AT1A and AT1B). The purpose of this study was to evaluate the effect of an AT1A homozygous deletion (AT1A-/-) on vascular reactivity. AT1A-/- mice and ... More
Sites of superoxide anion production detected by lucigenin in calf pulmonary artery smooth muscle.
AuthorsMohazzab KM, Wolin MS
JournalAm J Physiol
PubMed ID7810685
Sources of superoxide anion (O2-.) production in calf pulmonary artery smooth muscle homogenate and subcellular fractions were examined in this study by measurement of the chemiluminescence produced by the reaction of O2-. with 50 microM lucigenin, because recent evidence suggests that endogenously produced reactive O2 species appear to mediate certain ... More
Vacuolar chloride transport in Mesembryanthemum crystallinum L. measured using the fluorescent dye lucigenin.
AuthorsWissing F, Smith JA
JournalJ Membr Biol
PubMed ID11014858
To study vacuolar chloride (Cl(-)) transport in the halophilic plant Mesembryanthemum crystallinum L., Cl(-) uptake into isolated tonoplast vesicles was measured using the Cl(-)-sensitive fluorescent dye lucigenin (N,N'-dimethyl-9,9'-bisacridinium dinitrate). Lucigenin was used at excitation and emission wavelengths of 433 nm and 506 nm, respectively, and showed a high sensitivity towards ... More
Increased NADPH oxidase activity, gp91phox expression, and endothelium-dependent vasorelaxation during neointima formation in rabbits.
AuthorsParavicini TM, Gulluyan LM, Dusting GJ, Drummond GR
JournalCirc Res
PubMed ID12114322
Reactive oxygen species including superoxide and hydrogen peroxide are important mediators in atherogenesis. We investigated the enzymatic source of vascular superoxide and its role in endothelium-dependent vasorelaxation during neointima formation. Silastic collars positioned around carotid arteries of rabbits for 14 days induced neointimal thickening. Using lucigenin-enhanced chemiluminescence, superoxide production was ... More