LIVE BacLight™ 细菌革兰氏染色试剂盒，用于显微镜检查和定量检测

LIVE <i>Bac</i>Light™ 细菌革兰氏染色试剂盒，用于显微镜检查和定量检测

引用和文献 (7)

Invitrogen™

LIVE BacLight™ 细菌革兰氏染色试剂盒，用于显微镜检查和定量检测

LIVE BacLight™ 细菌革兰氏染色试剂盒提供了一种用于测定活体细菌中革兰氏染色反应的新型一步荧光检测法。当使用可渗透细胞膜的 SYTO® 9 染料及碘化己啶同时对革兰氏阴性菌和革兰氏阳性菌进行染色时，革兰氏阴性菌会发出绿色荧光，而革兰氏阳性菌会发出红色荧光。了解更多信息

货号	数量
L7005	1 kit

货号 L7005

价格（CNY)

8,186.00

添加至购物车

1 kit

价格（CNY)

8,186.00

添加至购物车

LIVE BacLight™ 细菌革兰氏染色试剂盒提供了一种用于测定活体细菌中革兰氏染色反应的新型一步荧光检测法。当使用可渗透细胞膜的 SYTO® 9 染料及碘化己啶同时对革兰氏阴性菌和革兰氏阳性菌进行染色时，革兰氏阴性菌会发出绿色荧光，而革兰氏阳性菌会发出红色荧光。

仅供科研使用。不可用于诊断程序。

描述LIVE BacLight™ 细菌革兰氏染色试剂盒，用于显微镜检查和定量测定

检测方法荧光

染料类型其他标记或染料

产品规格试管、96 孔板、玻片

数量1 kit

运输条件室温

颜色绿色、红色

发射480-700 nm

Excitation Wavelength Range470 nm

适用于（应用）活力测定试剂盒

适用于（设备）荧光显微镜, 荧光计, 流式细胞仪, 微孔板读数仪

产品线BacLight

产品类型细菌染色试剂盒

Unit SizeEach

内容与储存

在冷柜（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

我可以在流式细胞仪上看到哪些细菌参数?

您可以用BacLight Green细菌染色剂（货号B35000）或BacLight Red细菌染色剂（货号B35001）等常规染色剂进行细菌染色。你们可以看到革兰氏特性（货号L7005），细胞存活率（货号L7007、L7012和L13152），细胞计数（货号L34856和B7277）和细胞活力。细胞活力可以通过膜电位法（货号B34950）或代谢法（货号B34954和B34956）测定。

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission maxima for SYTO 9 stain and hexidium iodide?

The excitation/emission maxima for these dyes are 480/500 nm for SYTO 9 stain and 480/625 nm for hexidium iodide.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (7)

引用和文献

Abstract

Persistence and cell culturability of biocontrol strain Pseudomonas fluorescens CHA0 under plough pan conditions in soil and influence of the anaerobic regulator gene anr.

Authors:Mascher F, Schnider-Keel U, Haas D, Défago G, Moënne-Loccoz Y

Journal:Environ Microbiol

PubMed ID:12558593

'Certain fluorescent pseudomonads can protect plants from soil-borne pathogens, and it is important to understand how these biocontrol agents survive in soil. The persistence of the biocontrol strain Pseudomonas fluorescens CHA0-Rif under plough pan conditions was assessed in non-sterile soil microcosms by counting total cells (immunofluorescence microscopy), intact cells (BacLight ... More

Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.

Authors:Lebaron P, Parthuisot N, Catala P

Journal:Appl Environ Microbiol

PubMed ID:9572943

'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More

Determination of cell viability in single or mixed samples using capillary electrophoresis laser-induced fluorescence microfluidic systems.

Authors:Armstrong DW, He L

Journal:Anal Chem

PubMed ID:11605830

The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems. ... More

Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.

Authors:Durtschi JD, Erali M, Bromley LK, Herrmann MG, Petti CA, Smith RE, Voelkerding KV

Journal:J Med Microbiol

PubMed ID:16091435

A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects ... More

Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria.

Authors:Forster S, Snape JR, Lappin-Scott HM, Porter J

Journal:Appl Environ Microbiol

PubMed ID:12324319

Wastewater treatment is one of the most important commercial biotechnological processes, and yet the component bacterial populations and their associated metabolic activities are poorly understood. The novel fluorescent dye hexidium iodide allows assessment of Gram status by differential absorption through bacterial cell walls. Differentiation between gram-positive and gram-negative wastewater bacteria ... More

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