Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.
AuthorsLebaron P, Parthuisot N, Catala P
JournalAppl Environ Microbiol
PubMed ID9572943
'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More
Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting.
AuthorsNebe-von-Caron G, Stephens PJ, Hewitt CJ, Powell JR, Badley RA
JournalJ Microbiol Methods
PubMed ID11000436
With the increased awareness of the problems associated with the growth dependent analysis of bacterial populations, direct optical detection methods such as flow cytometry have enjoyed increased popularity over the last few years. Among the analyses discussed here are: (1) Bacterial discrimination from other particles on the basis of nucleic ... More