LIVE/DEAD™ 细胞介导的细胞毒性检测试剂盒，用于动物细胞

引用和文献 (14)

Invitrogen™

LIVE/DEAD™ 细胞介导的细胞毒性检测试剂盒，用于动物细胞

LIVE/DEAD® 细胞介导的细胞毒性检测试剂盒可测量自然杀伤 (NK) 细胞、淋巴因子激活的杀伤 (LAK) 细胞以及 T 细胞介导的细胞毒性。将靶标细胞与绿色荧光膜染色剂了解更多信息

货号	数量
L7010	1 kit

货号 L7010

价格（CNY)

4,915.00

添加至购物车

1 kit

价格（CNY)

4,915.00

添加至购物车

LIVE/DEAD® 细胞介导的细胞毒性检测试剂盒可测量自然杀伤 (NK) 细胞、淋巴因子激活的杀伤 (LAK) 细胞以及 T 细胞介导的细胞毒性。将靶标细胞与绿色荧光膜染色剂 DiOC₁₈ 预培养，然后在添加红色荧光、不可渗透细胞膜的染料（碘化丙锭）的情况下与效应细胞混合。存活和死亡的靶细胞均保留绿色荧光膜染色；细胞膜受损的靶细胞和效应细胞表现出红色荧光核酸染色；存活的效应细胞不发荧光。

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞、真核细胞

描述LIVE/DEAD™ 细胞介导的细胞毒性检测试剂盒，用于动物细胞

检测方法荧光

染料类型其他标记或染料

产品规格管、玻片

数量1 kit

运输条件室温

颜色绿色、红色

发射501、617 nm

Excitation Wavelength Range484、536 nm

适用于（应用）活力测定试剂盒

适用于（设备）荧光显微镜, 流式细胞仪

产品线LIVE/DEAD

产品类型细胞介导的细胞毒性试剂盒

Unit SizeEach

内容与储存

在冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (14)

引用和文献

Abstract

Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.

Authors:Ozdemir O,

Journal:J Immunol Methods

PubMed ID:17188705

'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this ... More

Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.

Authors:Bourke S, Mason HS, Borok Z, Kim KJ, Crandall ED, Kemp PJ,

Journal:Respir Res

PubMed ID:15857506

'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the ... More

Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.

Authors:Kroesen BJ, Mesander G, ter Haar JG, The TH, de Leij L

Journal:J Immunol Methods

PubMed ID:1431162

A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well ... More

Rapid flow cytometric assay for the assessment of natural killer cell activity.

Authors:Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK, Nehlsen-Cannarella SL

Journal:J Immunol Methods

PubMed ID:8228287

A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating ... More

Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.

Authors:

Journal:Int J Cancer

PubMed ID:23595559

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