Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More
Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More
Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).
AuthorsChalah T, Brillard JP
JournalTheriogenology
PubMed ID10732141
'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More
Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.
AuthorsJanett F, Thun R, Bettschen S, Burger D, Hassig M
JournalAnim Reprod Sci
PubMed ID12695055
'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
AuthorsKamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
JournalCytometry
PubMed ID10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More
Frequent structural chromosome aberrations in immotile human sperm exposed to culture media.
AuthorsWatanabe S
JournalHum Reprod
PubMed ID14990536
'BACKGROUND: The influence of culture media or centrifugation on chromosomes of immotile human sperm was examined using ICSI into mouse oocytes. METHODS: In experiment 1, immotile and motile human sperm retrieved directly from ejaculates were injected into mouse oocytes. In experiment 2, immotile human sperm were exposed to seminal plasma ... More
Protection of boar spermatozoa from cold shock damage by 2-hydroxypropyl-beta-cyclodextrin.
AuthorsZeng WX, Terada T
JournalTheriogenology
PubMed ID11233787
'This study examined whether 2-hydroxypropyl-beta-cyclodextrin (HBCD) could play a role in protecting spermatozoa from cold shock, as judged by motility parameters, intact acrosomes, and membrane integrity. Motility parameters were assessed by a computer-assisted sperm motility analysis (CASA) system, and the acrosome and membrane integrity were evaluated by fluorescent staining with ... More
The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa.
AuthorsFlajshans M, Cosson J, Rodina M, Linhart O
JournalCell Biol Int
PubMed ID15566965
'The viability of spermatozoa has been assessed using SYBR 14 staining for DNA of living cells and propidium iodide staining for DNA of degenerate cells. This dual staining was performed on four fish species (Siberian sturgeon, Acipenser baerii; common carp, Cyprinus carpio; tench, Tinca tinca and wels, Silurus glanis) and ... More
Incorporating lipids into boar sperm decreases chilling sensitivity but not capacitation potential.
AuthorsHe L, Bailey JL, Buhr MM
JournalBiol Reprod
PubMed ID11133660
'Fresh boar sperm were incubated with small unilamellar liposomes composed of either the total lipids extracted from head plasma membranes (HPM) of fresh boar sperm or selected lipids (SL) of five defined phospholipids with specific acyl chains. To optimize fusion, liposomes with 2 mol% octadecyl rhodamine fluorophore in Beltsville Thawing ... More
Flow cytometric evaluation of antibiotic effects on viability and mitochondrial function of refrigerated spermatozoa of Nile tilapia.
AuthorsSegovia M, Jenkins JA, Paniagua-Chavez C, Tiersch TR
JournalTheriogenology
PubMed ID10898218
'Improved techniques for storage and evaluation of fish sperm would enhance breeding programs around the world. The goal of this study was to test the effect of antibiotics on refrigerated sperm from Nile tilapia (Oreochromis niloticus) by use of flow cytometry with 2 dual-staining protocols for objective assessment of sperm ... More
Effects of water- and lipid-soluble antioxidants on turkey sperm viability, membrane integrity, and motility during liquid storage.
AuthorsDonoghue AM, Donoghue DJ
JournalPoult Sci
PubMed ID9316122
'Aerobic conditions are required to maintain the viability of turkey sperm in vitro. In mammalian sperm, excess oxygen during in vitro storage results in lipid peroxidation, causing membrane damage and reduced sperm motility and subsequent fertility. The effect of adding antioxidants to turkey sperm during liquid storage was studied. Semen ... More
Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification.
AuthorsFigueroa E, Merino O, Risopatrón J, Isachenko V, Sánchez R, Effer B, Isachenko E, Farias JG, Valdebenito I,
Journal
PubMed ID25442390
'This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0.13-M sucrose + ... More
Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of Leishmania infantum promastigotes.
AuthorsKamau SW, Nunez R, Grimm F
JournalBMC Pharmacol
PubMed ID11299045
'BACKGROUND: Leishmaniasis is a major parasitic disease in the tropical regions. However, Leishmania infantum has recently emerged as a very important cause of opportunistic infections for individuals positive for human immunodeficiency virus (HIV). However, there is a lack of in vitro tests for assessing the effect of anti-parasitic drugs on ... More
Cell damage by near-IR microbeams.
AuthorsKönig K, Liang H, Berns MW, Tromberg BJ
JournalNature
PubMed ID7659153
Influence of Eqvalan (ivermectin) on quality and freezability of stallion semen.
AuthorsJanett F, Thun R, Ryhiner A, Burger D, Hassig M, Hertzberg H
JournalTheriogenology
PubMed ID11245265
'The objective of this study was to evaluate the effect of Eqvalan (ivermectin) on stallion semen quality and freezability. Experiments were performed using 22 Freiberger stallions, randomly divided into a control and test group. Semen was collected once a week for 17 weeks from October 1997 to February 1998. Eqvalan ... More
Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.
AuthorsGarner DL, Johnson LA
JournalBiol Reprod
PubMed ID7492679
'The proportion of living sperm in semen from six representative mammals was assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). SYBR-14, a newly developed fluorescent nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. ... More
Optimal utilization of cryopreserved human semen for assisted reproduction: recovery and maintenance of sperm motility and viability.
AuthorsPolcz TE, Stronk J, Xiong C, Jones EE, Olive DL, Huszar G
JournalJ Assist Reprod Genet
PubMed ID9785198
'PURPOSE: Our purpose was to evaluate sperm motility and viability and the maintenance of these parameters in already cryopreserved semen samples following repeated freezing/thawing cycles. METHODS: Human spermatozoa were subjected to five cycles of cryopreservation/thawing. Recovery of sperm motility and viability and the proportion of viable nonmotile sperm were determined ... More
Viability and membrane integrity of spermatozoa after dilution and flow cytometric sorting in the presence or absence of seminal plasma.
AuthorsMaxwell WM, Welch GR, Johnson LA
JournalReprod Fertil Dev
PubMed ID8981641
'Boar, bull and ram spermatozoa were examined after staining with the DNA-permeant Hoechst 33342 fluorochrome and flow cytometric sorting in the presence or absence of seminal plasma. Spermatozoa were assessed for viability with flow cytometry using the live cell nucleic acid stain SYBR-14 and propidium iodide (PI), and for membrane ... More
Comparative genomic hybridization analysis of sperm DNA apoptosis after exposure to heat shock.
AuthorsMann SL, Patton WC, King A, Chan PJ
JournalJ Assist Reprod Genet
PubMed ID12036087
'PURPOSE: A DNA disc chip assay based on comparative genomic hybridization (CGH) was developed to measure sperm DNA integrity. The objective was to correlate DNA integrity of heat-treated sperm with the sperm capacitation index (CI) determined from the sperm penetration assay. METHODS: Basic semen and kinematic parameters were measured (N ... More
Viability assessment of honey bee, Apis mellifera, sperm using dual fluorescent staining.
AuthorsCollins AM, Donoghue AM
JournalTheriogenology
PubMed ID10729078
'Since the development of instrumental insemination of honey bee (Apis mellifera) queens in the 1930s, there has been interest in the evaluation and in vitro storage of semen. Several fluorescent stains, when used in combination, have been effectively used to assess sperm viability in mammalian and avian species. Our objectives ... More
Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function.
AuthorsGravance CG, Garner DL, Miller MG, Berger T
JournalReprod Toxicol
PubMed ID11137373
'Fluorescent assessment of cellular integrity and mitochondrial function by flow cytometry can provide a rapid and precise means of determining the functional status of large numbers of spermatozoa. In the present study, rat sperm viability was assessed with SYBR-14 and PI and sperm mitochondria were differentially labeled with JC-1. Sperm ... More
Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry.
Relationships between sperm motility parameters and viability were evaluated using two fluorescent staining techniques in fresh extended semen (fresh and after 24 h storage at 5 degrees C) that had various concentrations of dead sperm added to simulate different levels of viable and nonviable sperm. Both protocols incorporated SYBR-14 and ... More
Combining reduced glutathione and ascorbic acid has supplementary beneficial effects on boar sperm cryotolerance.
AuthorsGiaretta E, Estrada E, Bucci D, Spinaci M, Rodríguez-Gil JE, Yeste M,
Journal
PubMed ID25459422
The main aim of this work was to evaluate how supplementing freezing and thawing media with reduced glutathione (GSH) and l-ascorbic acid (AA) affected the quality parameters of frozen-thawed boar spermatozoa. With this purpose, semen samples of 12 ejaculates coming from 12 boars were used. Each ejaculate was split into ... More
A comparison of alternative assays to measure DNA damage in stallion spermatozoa: TUNEL test versus 'Nicoletti assay'.
AuthorsKakasi B, Nagy S, Pál L, Czimber GE, Husvéth F,
Journal
PubMed ID25655419
The aberrations of sperm DNA may cause various problems and have negative consequences on fertility. These influence embryonic development or might lead to early embryo loss. Sperm Chromatin Structure Assay (SCSA) is the flow cytometric method most often used for the detection of DNA lesions; however, some studies using that ... More
In vitro exposure to the organochlorine p,p'-DDE affects functional human sperm parameters.
AuthorsTavares RS, Amaral S, Paiva C, Baptista M, Ramalho-Santos J,
Journal
PubMed ID25240159
Although no information exists regarding the levels of p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) on reproductive fluids of heavily exposed populations, they are possibly quite high given the serum levels reported so far. In these populations altered semen quality has been reported, although the direct effects of this DDT metabolite on crucial sperm parameters ... More
Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa.
Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris.HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was not destroyed by freeze-drying or freezing without cryoprotection in this solution. Embryos derived after injecting ... More
Quantitative monitoring of the mRNA expression pattern of the TGF-beta-isoforms (beta 1, beta 2, beta 3) during transdifferentiation of hepatic stellate cells using a newly developed real-time SYBR Green PCR.
AuthorsWickert L, Steinkrüger S, Abiaka M, Bolkenius U, Purps O, Schnabel C, Gressner AM
JournalBiochem Biophys Res Commun
PubMed ID12150952
Current methods to determine the mRNA of the TGF-beta-isoforms, beta 1, beta 2, and beta 3, are not sensitive enough to detect small alterations in the expression levels. Therefore, we established a SYBR Green I-based real-time quantitative PCR procedure with fragment-specific standards. The advantage of gene-specific quantification is the possibility ... More
Electrophoretic behavior and potency assessment of boar sperm using a capillary electrophoresis-laser induced fluorescence system.
AuthorsHe L, Jepsen RJ, Evans LE, Armstrong DW
JournalAnal Chem
PubMed ID12622373
The assessment of mammalian fertility, and the agents that affect it, is of increasing concern in medicinal, environmental, and agricultural science. The viability, integrity, and overall state of the male gamete (sperm) is an essential factor that must be considered in such studies. Traditional potency evaluations tend to be labor ... More
Comparison of motility and membrane integrity to assess rat sperm viability.
AuthorsVetter CM, Miller JE, Crawford LM, Armstrong MJ, Clair JH, Conner MW, Wise LD, Skopek TR
JournalReprod Toxicol
PubMed ID9535503
Rat sperm motility and membrane integrity were compared as endpoints for viability. Sperm motility was measured by computer-assisted semen analysis (CASA), whereas membrane integrity was assessed by flow cytometric analysis of sperm stained with two nucleic acid stains, SYBR-14 and propidium iodide. The two techniques were compared in experiments that ... More
Survival of boar spermatozoa frozen in diluents of varying osmolality.
AuthorsZeng WX, Shimada M, Isobe N, Terada T
JournalTheriogenology
PubMed ID11516124
We investigated the effects of freezing diluents of differing levels of osmolality on boar sperm cryosurvival. The spermatozoa were frozen using a pellet technique. Cryosurvival was evaluated in terms of motility, intact acrosomes and membrane integrity. The motility parameters were assessed using a computer-assisted sperm motility analysis (CASA) system. Acrosomal ... More
Sperm apoptosis in nonpregnant luteal phase sera after in vitro fertilization as assessed by comparative genomic hybridization.
AuthorsBouma CL, Patton WC, Jacobson JD, King A, Chan PJ
JournalArch Androl
PubMed ID14660171
Toxicity in serum has been reported in cases of recurrent spontaneous abortions and endometriosis. The null hypothesis was that serum toxicity was not involved in failed pregnancies after in vitro fertilization procedures. The objective was to expose donor sperm to pregnant versus nonpregnant patient sera and analyze for sperm DNA ... More
Survival of honey bee (Hymenoptera: Apidae) spermatozoa stored at above-freezing temperatures.
AuthorsCollins AM
JournalJ Econ Entomol
PubMed ID10902300
The development of practical techniques for the storage of honey bee, Apis mellifera L., semen would significantly improve our ability to breed for desirable genotypes and maintain genetic diversity in populations. Artificial insemination of queens has been possible for some time, but the semen used is usually freshly collected, or ... More
Organelle-specific probe JC-1 identifies membrane potential differences in the mitochondrial function of bovine sperm.
AuthorsGarner DL, Thomas CA
JournalMol Reprod Dev
PubMed ID10331460
The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differentiates these from mitochondria exhibiting relatively high membrane potentials. This discrimination occurs because JC-1 forms aggregates at high membrane potentials. These J-aggregates emit a ... More
Birth of live mice resulting from oocytes fertilized in vitro with cryopreserved spermatozoa.
AuthorsSongsasen N, Betteridge KJ, Leibo SP
JournalBiol Reprod
PubMed ID9002643
The objective of this study was to develop a reliable method to cryopreserve mouse spermatozoa, using in vitro fertilization (IVF) of oocytes, development of resultant zygotes into blastocysts, and the birth of live young after embryo transfer as criteria of sperm survival. Epididymal sperm were frozen in a raffinose-glycerol solution ... More
Changes in plasma membrane and acrosome integrity of frozen-thawed bovine spermatozoa during a 4 h incubation as measured by multicolor flow cytometry.
AuthorsNagy S, Hallap T, Johannisson A, Rodriguez-Martinez H
JournalAnim Reprod Sci
PubMed ID15036499
Cryopreservation of bull semen is sub-optimal, causing cell death of a majority of spermatozoa. Even the surviving cells are affected post-thaw, either structurally or functionally. The aim of this study was to investigate the sequence of events that take place when sperm plasma membrane and acrosome deteriorate during a 4 ... More
Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry.
AuthorsThomas CA, Garner DL, DeJarnette JM, Marshall CE
JournalBiol Reprod
PubMed ID9510967
Flow cytometry was used to compare the functional status of fluorescently stained sperm organelles from 12 Holstein bulls after storage for 24 h at 5 degrees C and after cryopreservation. The organelle-specific stains, SYBR-14 and LysoTracker Green DND-26, identified spermatozoa with intact plasmalemma and those with intact acrosomes, respectively. The ... More
Fluorometric assessments of acrosomal integrity and viability in cryopreserved bovine spermatozoa.
AuthorsThomas CA, Garner DL, DeJarnette JM, Marshall CE
JournalBiol Reprod
PubMed ID9096883
The combination of specific fluorometric staining and flow cytometry provides a rapid and precise means of assessing the functional status of cells. We sought to utilize this approach to quantify two important seminal characteristics, acrosomal integrity and sperm viability, and to compare these with classical microscopic measurements of acrosomal integrity ... More
A flow cytometric method for rapid determination of sperm concentration and viability in mammalian and avian semen.
AuthorsChristensen P, Stenvang JP, Godfrey WL
JournalJ Androl
PubMed ID14760012
A new flow cytometric method has been developed to rapidly determine sperm concentration and viability in semen from bulls and boars. Sperm viability was determined on the basis of staining with SYBR-14 and propidium iodide (PI), and this allowed detection of live (membrane-intact) sperm, dying (moribund) sperm, as well as ... More
Viability assessment of turkey sperm using fluorescent staining and flow cytometry.
AuthorsDonoghue AM, Garner DL, Donoghue DJ, Johnson LA
JournalPoult Sci
PubMed ID7479495
Turkey sperm viability was evaluated using several fluorescent stains both singularly and in combination. Dilution curves and several extenders were used to determine optimal stain concentrations. Semen was collected from eight toms, pooled, diluted, stained, and evaluated microscopically within 2 h of collection. Replicates were assessed for both viable and ... More
Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.
AuthorsGarner DL, Thomas CA, Joerg HW, DeJarnette JM, Marshall CE
JournalBiol Reprod
PubMed ID9408246
Mitochondrial function and sperm viability were quantified in samples of cryopreserved bovine spermatozoa from 12 bulls using fluorometric techniques. The active mitochondria of the spermatozoa were fluorescently stained using three different fluorophores: rhodamine 123 (R123), 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) or MitoTracker Green FM (MITO). The stained spermatozoa, and companion aliquots ... More
Description of a flow cytometry approach based on SYBR-14 staining for the assessment of DNA content, cell cycle analysis, and sorting of living normal and neoplastic cells.
AuthorsNunez R, Garay N, Villafane C, Bruno A, Lindgren V
JournalExp Mol Pathol
PubMed ID14738866
RATIONALE: This study aimed to expand the utilization of a simplified flow cytometric approach that employing SYBR-14/PI staining into broader flow cytometry applications, including (i) measurement of the DNA content; (ii) performing cell cycle analysis on mammalian cells; and (iii) sorting of live SYBR-14-stained mammalian cells based on DNA content. ... More
Fertility of ram semen frozen in Bioexcell and used for cervical artificial insemination.
AuthorsGil J, Rodriguez-Irazoqui M, Lundeheim N, Söderquist L, Rodríguez-Martínez H
JournalTheriogenology
PubMed ID12527064
The current use of ingredients of animal origin, such as egg yolk, in semen extenders presents a risk of microbial contamination, and has led to the search for alternatives. Such an extender is commercially available for bull semen (Bioexcell), IMV, L'Aigle, France), and it has previously been tested in vitro ... More
Influence of centrifugation or low extension rates prefreezing on the fertility of ram semen after cervical insemination.
AuthorsGil J, Rodriguez-Irazoqui M, Söderquist L, Rodriguez-Martinez H
JournalTheriogenology
PubMed ID12041682
We compared the fertility of thawed ram semen, frozen according to different prefreezing semen handling protocols and previously well-defined in vitro, after cervical artificial insemination (AI) during natural estrus in Corriedale sheep. Following primary extension 1 + 1, we adjusted the final sperm concentration before packaging (200 x 10(6)/straw) either ... More
Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling, or cryopreservation.
AuthorsMaxwell WM, Johnson LA
JournalMol Reprod Dev
PubMed ID9041145
In this study, the effects of staining procedure with chlortetracycline (CTC) and method of analysis of boar spermatozoa after staining were examined. The hypothesis that incubation, flow cytometric sorting, cooling, and cryopreservation cause changes to boar sperm membranes which resemble capacitation and the acrosome reaction was also tested. Membrane status ... More
Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle, and peregrine falcon spermatozoa.
AuthorsBlanco JM, Gee G, Wildt DE, Donoghue AM
JournalBiol Reprod
PubMed ID10993841
Potential factors influencing spermatozoa survival to cryopreservation and thawing were analyzed across a range of the following avian species: domestic chicken (Gallus domesticus), domestic turkey (Meleagris gallopavo), golden eagle (Aquila chrysaetos), Bonelli's eagle (Hieraaetus fasciatus), imperial eagle (Aquila adalberti), and peregrine falcon (Falco peregrinus). Studies focused on spermatozoa tolerance to ... More
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry.
AuthorsGarner DL, Thomas CA, Allen CH
JournalJ Androl
PubMed ID9203062
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull was collected on all four collection dates as a control for effects of collection date. The ejaculates from ... More
Post-thaw functional status of boar spermatozoa cryopreserved using three controlled rate freezers: a comparison.
AuthorsThurston LM, Holt WV, Watson PF
JournalTheriogenology
PubMed ID12620584
This study compared variation in the quality of cryopreserved boar spermatozoa and the control and accuracy of cooling rates between three semen freezers (CryoLogic Freeze Control CL3000, Planer Products Kryo Save Compact KS1.7/Kryo 10 Control module and a controlled rate 'Watson' freezing machine developed within our laboratory). Five ejaculates were ... More
Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate.
The objectives of this study were to compare different methods of evaluating sperm plasmalemma and to determine their relationship with in vitro fertilization rate. A single batch of frozen semen from each of eight beef bulls was used for assessment of sperm viability and for in vitro fertilization. Conventional viability ... More
Influence of centrifugation and different extenders on post-thaw sperm quality of ram semen.
AuthorsGil J, Söderquist L, Rodriguez-Martinez H
JournalTheriogenology
PubMed ID10990351
Using a 2-step extension methodology to freeze ram semen, 2 freezing protocols (P1 and P2) and 3 extenders were evaluated in a split-sample experiment. The freezing protocols were tested in combination with Extenders A and B (Experiment 1), and B and C (Experiment 2). Protocol 1 included centrifugation before filling ... More
Effect of pentoxifylline on tumor suppressor and proto-oncogene apoptosis in sperm.
AuthorsMaxwell DT, Jacobson JD, King A, Chan PJ
JournalJ Assist Reprod Genet
PubMed ID12166633
PURPOSE: Pentoxifylline (PTX), a methylxanthine phosphodiesterase inhibitor reduces superoxide anions responsible for DNA apoptosis. The null hypothesis was that PTX was equally effective in reducing damage to specific cell genes. The objective was to determine the DNA integrity of the BRCA1 tumor suppressor gene and the c-myc proto-oncogene after PTX. ... More
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide.
AuthorsGarner DL, Johnson LA, Yue ST, Roth BL, Haugland RP
JournalJ Androl
PubMed ID7721666
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI only stained degenerate cells that had lost their membrane integrity. Staining with SYBR-14 resulted in the nuclei of living ... More
Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen.
AuthorsGil J, Lundeheim N, Söderquist L, Rodriíuez-Martínez H
JournalTheriogenology
PubMed ID12527072
Using a two-step extension methodology, two experiments were conducted using a split-sample design to compare the effect on post-thaw ram sperm parameters of a milk-based extender (Experiment 1) containing four different egg yolk concentrations (5% [M5], 10% [M10], 15% [M15], and 20% [M20]), and a commercially available extender (Bioexcell); IMV, ... More
Semen characteristics after vasectomy in the ram.
AuthorsJanett F, Hüssy D, Lischer C, Hässig M, Thun R
JournalTheriogenology
PubMed ID11516127
The objective of this study was to monitor the changes in semen characteristics in vasectomized rams and to determine if infertility was present 14 days after vasectomy. Experiments were performed using five cross-breed rams, aged between 18 and 30 months. Semen was collected weekly by artificial vagina from 2 months ... More
A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles.
AuthorsNagy S, Jansen J, Topper EK, Gadella BM
JournalBiol Reprod
PubMed ID12606354
Simultaneously evaluating postthaw viability and acrosome integrity of spermatozoa by flow cytometry would provide a valuable testing tool in both research and routine work. In the present study, a new triple-stain combination was developed for the simultaneous evaluation of viability and acrosome integrity of bovine sperm processed in egg yolk-based ... More
Effect of cooling rates on post-thaw sperm motility, membrane integrity, capacitation status and fertility of dairy bull semen used for artificial insemination in Sweden.
AuthorsJanuskauskas A, Gil J, Söderquist L, Håård MG, Håård MC, Johannisson A, Rodriguez-Martinez H
JournalTheriogenology
PubMed ID10734363
We studied the effects of 2 different cooling rates during equilibration of semen from room temperature to 4 degrees C, at 4.2 degrees C/min (control split sample) or at 0.1 degree C/min (treatment split sample) on in vitro sperm viability post thawing and fertility after AI. Forty batches of split-frozen ... More
Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro.
AuthorsByrne GP, Lonergan P, Wade M, Duffy P, Donovan A, Hanrahan JP, Boland MP
JournalAnim Reprod Sci
PubMed ID10924829
Ram spermatozoa are most susceptible to damage during freezing between the temperatures of -10 degrees C and -25 degrees C. The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen ... More
Validation of the FACSCount AF system for determination of sperm concentration in boar semen.
AuthorsHansen C, Christensen P, Stryhn H, Hedeboe AM, Rode M, Boe-Hansen G
JournalReprod Domest Anim
PubMed ID12464070
A flow cytometric method has been developed for rapid determination of sperm concentration in semen from various mammalian species.* All cells containing DNA are stained with SYBR-14 or propidium iodide (PI) and sperm concentration is determined in relation to an internal standard of fluorescent microspheres (beads). Satisfactory staining can be ... More
In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo.
AuthorsChalah T, Seigneurin F, Blesbois E, Brillard JP
JournalCryobiology
PubMed ID10529312
A series of experiments was conducted to compare the viability of fresh fowl spermatozoa, samples suspended in three cryoprotectants (CPAs), frozen/thawed samples, and frozen/thawed samples maintained in vitro for up to 24 h. The CPAs used were glycerol (Glyc), dimethylacetamide (DMA), and dimethylformamide (DMF). Viability was assayed using two double ... More
Seasonal changes in semen quality and freezability in the Warmblood stallion.
AuthorsJanett F, Thun R, Niederer K, Burger D, Hässig M
JournalTheriogenology
PubMed ID12763159
The objective of this study was to investigate seasonal changes in stallion semen quality and to determine the best time for semen cryopreservation. Experiments were performed using 10 Warmblood stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and frozen every other week during 1 year from ... More
Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways.
Authors
JournalSci Rep
PubMed ID27883089
Resveratrol Improves Boar Sperm Quality via 5'AMP-Activated Protein Kinase Activation during Cryopreservation.
Authors
JournalOxid Med Cell Longev
PubMed ID31565152
Post-thaw ATP supplementation enhances cryoprotective effect of iodixanol in rat spermatozoa.
Authors
JournalReprod Biol Endocrinol
PubMed ID26821719
Introduction of Varroa destructor has not altered honey bee queen mating success in the Hawaiian archipelago.
Authors
JournalSci Rep
PubMed ID33446846
Impact of Segmented Magnetization on the Flagellar Propulsion of Sperm-Templated Microrobots.
Authors
JournalAdv Sci (Weinh)
PubMed ID33898186
Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm.
Authors
JournalFront Physiol
PubMed ID30914972
Glycogen Synthase Kinase-3 Regulates Sperm Motility and Acrosome Reaction via Affecting Energy Metabolism in Goats.
Authors
JournalFront Physiol
PubMed ID31417426
Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility.
Authors
JournalSci Rep
PubMed ID27633131
A testis-expressing heme peroxidase HPX12 regulates male fertility in the mosquito Anopheles stephensi.
Authors
JournalSci Rep
PubMed ID35173215
A phenotypic screening platform utilising human spermatozoa identifies compounds with contraceptive activity.