An easily synthesized, photolyzable luciferase substrate for in vivo luciferase activity measurement.
AuthorsYang J, Thomason DB
JournalBiotechniques
PubMed ID8267979
'Many reporter gene assays require killing the cell by fixation or lysis. For assays in living cells, the substrate delivery is inefficient and cannot be supplied in situ in a bolus, which makes assays highly variable. We report a simple synthesis of a luciferin ester that is both photolyzable and ... More
Lysine N(epsilon)-trimethylation, a tool for improving the selectivity of antimicrobial peptides.
AuthorsFernández-Reyes M, Díaz D, de la Torre BG, Cabrales-Rico A, Vallès-Miret M, Jiménez-Barbero J, Andreu D, Rivas L,
JournalJ Med Chem
PubMed ID20617807
'The effects of lysine N(epsilon)-trimethylation at selected positions of the antimicrobial cecropin A-melittin hybrid peptide KWKLFKKIGAVLKVL-amide have been studied. All five monotrimethylated, four bis-trimethylated plus the per-trimethylated analogues have been synthesized and tested for antimicrobial activity on Leishmania parasites and on Gram-positive and -negative bacteria, as well as for hemolysis ... More
Caged ATP - an internal calibration method for ATP bioluminescence assays.
ATP bioluminescence, based on the firefly luciferase system, is used for the rapid determination of hygienic practices in the food industry. This study has demonstrated the use of caged ATP as an internal ATP standard and quantified the effects of industrial cleansing solutions, alcoholic beverages and pH on firefly luciferase ... More