Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay.
AuthorsDemo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, Scheller RH, Fisher JM
JournalCytometry
PubMed ID10404150
'BACKGROUND: Mast cells are primary mediators of allergic inflammation. Antigen-mediated crosslinking of their cell surface immunoglobulin E (IgE) receptors results in degranulation and the release of proinflammatory mediators including histamine, tumor necrosis factor-alpha, and leukotrienes. METHODS: Mast cells were stimulated to degranulate by using either IgE crosslinking or ionophore treatment. ... More
Determination of intracellular organelles implicated in daunorubicin cytoplasmic sequestration in multidrug-resistant MCF-7 cells using fluorescence microscopy image analysis.
AuthorsBour-Dill C, Gramain MP, Merlin JL, Marchal S, Guillemin F
JournalCytometry
PubMed ID10655559
'BACKGROUND: Anthracycline resistance is known to be mediated by P-glycoprotein (P-gp) or multidrug-resistance related protein (MRP) as well as intracellular sequestration of drugs. METHODS: The resistance phenotype of doxorubicin-selected MCF-7(DXR) human breast adenocarcinoma cell line was characterized by cellular and nuclear daunorubicin efflux, P-gp and MRP expression and apoptosis induction. ... More
In situ assessment of cell viability.
AuthorsYang H, Acker J, Chen A, McGann L
JournalCell Transplant
PubMed ID9786064
'Cryobiological studies of tissues often require the simultaneous assessment of tissue structure and in situ cellular function. Localization of damage during cryopreservation occurs as a consequence of tissue structure and morphology and as a result of biophysical constraints imposed by diffusion and heat transfer. This study used five experimental model ... More
Higher order Rab programming in phagolysosome biogenesis.
AuthorsRoberts EA, Chua J, Kyei GB, Deretic V
JournalJ Cell Biol
PubMed ID16982798
'Phagosomes offer kinetically and morphologically tractable organelles to dissect the control of phagolysosome biogenesis by Rab GTPases. Model phagosomes harboring latex beads undergo a coordinated Rab5-Rab7 exchange, which is akin to the process of endosomal Rab conversion, the control mechanisms of which are unknown. In the process of blocking phagosomal ... More
In vitro and in vivo photocytotoxicity of boron dipyrromethene derivatives for photodynamic therapy.
AuthorsLim SH, Thivierge C, Nowak-Sliwinska P, Han J, van den Bergh H, Wagnières G, Burgess K, Lee HB,
JournalJ Med Chem
PubMed ID20199028
To understand the effects of substitution patterns on photosensitizing the ability of boron dipyrromethene (BODIPY), two structural variations that either investigate the effectiveness of various iodinated derivatives to maximize the ... More
Utilization of fluorescent probes for the quantification and identification of subcellular proteomes and biological processes regulated by lipid peroxidation products.
AuthorsCummins TD, Higdon AN, Kramer PA, Chacko BK, Riggs DW, Salabei JK, Dell'italia LJ, Zhang J, Darley-Usmar VM, Hill BG,
JournalFree Radic Biol Med
PubMed ID22954622
Oxidative modifications to cellular proteins are critical in mediating redox-sensitive processes such as autophagy, the antioxidant response, and apoptosis. The proteins that become modified by reactive species are often compartmentalized to specific organelles or regions of the cell. Here, we detail protocols for identifying the subcellular protein targets of lipid ... More
Prolylcarboxypeptidase regulates proliferation, autophagy, and resistance to 4-hydroxytamoxifen-induced cytotoxicity in estrogen receptor-positive breast cancer cells.
AuthorsDuan L, Motchoulski N, Danzer B, Davidovich I, Shariat-Madar Z, Levenson VV,
JournalJ Biol Chem
PubMed ID21087932
Endocrine therapy with tamoxifen (TAM) significantly improves outcomes for patients with estrogen receptor-positive breast cancer. However, intrinsic (de novo) or acquired resistance to TAM occurs in a significant proportion of treated patients. To identify genes involved in resistance to TAM, we introduced full-length cDNA expression library into estrogen receptor-positive MCF7 ... More
Characterization of lysosomal contribution to whole-cell light scattering by organelle ablation.
AuthorsWilson JD, Foster TH,
JournalJ Biomed Opt
PubMed ID17614706
Angularly resolved light scattering measurements made at visible wavelengths have the ability to quantify subcellular morphology, with particular sensitivity to organelles the size of mitochondria and lysosomes. We have recently reported on a lysosome-staining-based method that provides scattering contrast between stained and unstained cells, and through the use of appropriate ... More
PEGylated liposomal doxorubicin targeted to a5ß1-expressing MDA-MB-231 breast cancer cells.
AuthorsShroff K, Kokkoli E,
JournalLangmuir
PubMed ID22268611
Targeting drugs selectively to cancer cells can potentially benefit cancer patients by avoiding side effects generally associated with several cancer therapies. One of the attractive approaches to direct the drug cargo to specific sites is to incorporate ligands at the surface of the delivery systems. Integrin a(5)ß(1) is overexpressed in ... More
Zinc dyshomeostasis is linked with the loss of mucolipidosis IV-associated TRPML1 ion channel.
AuthorsEichelsdoerfer JL, Evans JA, Slaugenhaupt SA, Cuajungco MP,
JournalJ Biol Chem
PubMed ID20864526
Chelatable zinc is important in brain function, and its homeostasis is maintained to prevent cytotoxic overload. However, certain pathologic events result in intracellular zinc accumulation in lysosomes and mitochondria. Abnormal lysosomes and mitochondria are common features of the human lysosomal storage disorder known as mucolipidosis IV (MLIV). MLIV is caused ... More
Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug.
The dose-limiting toxicity of chemotherapeutics, heterogeneity and drug resistance of cancer cells, and difficulties of targeted delivery to tumors all pose daunting challenges to effective cancer therapy. We report that small interfering RNA (siRNA) duplexes readily penetrate intact bacterially derived minicells previously shown to cause tumor stabilization and regression when ... More
A lysosomal tetraspanin associated with retinal degeneration identified via a genome-wide screen.
AuthorsXu H, Lee SJ, Suzuki E, Dugan KD, Stoddard A, Li HS, Chodosh LA, Montell C
JournalEMBO J
PubMed ID14963491
The Drosophila visual system has provided a model to study phototransduction and retinal degeneration. To identify new candidate proteins that contribute to these processes, we conducted a genome-wide screen for genes expressed predominately in the eye, using DNA microarrays. This screen appeared to be comprehensive as it led to the ... More
Cationic albumin-conjugated pegylated nanoparticles allow gene delivery into brain tumors via intravenous administration.
AuthorsLu W, Sun Q, Wan J, She Z, Jiang XG
JournalCancer Res
PubMed ID17178885
Patients with malignant gliomas have a poor prognosis because these tumors do not respond well to conventional treatments. Studies of glioma xenografts suggest that they may be amenable to gene therapy with cytotoxic genes, such as the proapoptotic Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Gene therapy of gliomas ideally ... More
EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes.
Authors
JournalNucleic Acids Res
PubMed ID32313933
Alpha-synuclein fibrils recruit TBK1 and OPTN to lysosomal damage sites and induce autophagy in microglial cells.
AuthorsBussi C, Peralta Ramos JM, Arroyo DS, Gallea JI, Ronchi P, Kolovou A, Wang JM, Florey O, Celej MS, Schwab Y, Ktistakis NT, Iribarren P
JournalJ Cell Sci
PubMed ID30404831
'Autophagic dysfunction and protein aggregation have been linked to several neurodegenerative disorders, but the exact mechanisms and causal connections are not clear and most previous work was done in neurons and not in microglial cells. Here, we report that exogenous fibrillary, but not monomeric, alpha-synuclein (AS, also known as SNCA) ... More
The translocator protein (TSPO) is prodromal to mitophagy loss in neurotoxicity.
Authors
JournalMol Psychiatry
PubMed ID33664474
Multicolor polymeric carbon dots: synthesis, separation and polyamide-supported molecular fluorescence.
Authors
JournalChem Sci
PubMed ID34164010
Synthesis of marmycin A and investigation into its cellular activity.
Authors
JournalNat Chem
PubMed ID26291947
Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.
Authors
JournalACS Nano
PubMed ID32940450
Live Imaging of Label-Free Graphene Oxide Reveals Critical Factors Causing Oxidative-Stress-Mediated Cellular Responses.
Authors
JournalACS Nano
PubMed ID29286639
Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide.
Authors
JournalNat Commun
PubMed ID34155215
Spatiotemporally controlled induction of autophagy-mediated lysosome turnover.
Authors
JournalNat Commun
PubMed ID23817530
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.
Authors
JournalNat Protoc
PubMed ID31996843
A Comparative Study of Cellular Uptake and Subcellular Localization of Doxorubicin Loaded in Self-Assemblies of Amphiphilic Copolymers with Pendant Dendron by MDA-MB-231 Human Breast Cancer Cells.
AuthorsViswanathan G, Hsu YH, Voon SH, Imae T, Siriviriyanun A, Lee HB, Kiew LV, Chung LY, Yusa S
JournalMacromol Biosci
PubMed ID26900760
Previously synthesized amphiphilic diblock copolymers with pendant dendron moieties have been investigated for their potential use as drug carriers to improve the delivery of an anticancer drug to human breast cancer cells. Diblock copolymer (P71 D3 )-based micelles effectively encapsulate the doxorubicin (DOX) with a high drug-loading capacity (˜95%, 104 ... More
Sphingolipid Modulation Activates Proteostasis Programs to Govern Human Hematopoietic Stem Cell Self-Renewal.
AuthorsXie SZ, Garcia-Prat L, Voisin V, Ferrari R, Gan OI, Wagenblast E, Kaufmann KB, Zeng AGX, Takayanagi SI, Patel I, Lee EK, Jargstorf J, Holmes G, Romm G, Pan K, Shoong M, Vedi A, Luberto C, Minden MD, Bader GD, Laurenti E, Dick JE
JournalCell Stem Cell
PubMed ID31631013
Cellular stress responses serve as crucial decision points balancing persistence or culling of hematopoietic stem cells (HSCs) for lifelong blood production. Although strong stressors cull HSCs, the linkage between stress programs and self-renewal properties that underlie human HSC maintenance remains unknown, particularly at quiescence exit when HSCs must also dynamically ... More
EGFR TKIs impair lysosome-dependent degradation of SQSTM1 to compromise the effectiveness in lung cancer.
AuthorsYang L, Ying S, Hu S, Zhao X, Li M, Chen M, Zhu Y, Song P, Zhu L, Jiang T, An H, Yousafzai NA, Xu W, Zhang Z, Wang X, Feng L, Jin H
JournalSignal Transduct Target Ther
PubMed ID31637005
Tyrosine kinase inhibitors for epidermal growth factor receptor (EGFR TKIs) greatly improved clinical outcomes of patients with non-small cell lung cancer (NSCLC). Unfortunately, primary and acquired resistance limits their clinical benefits. To overcome such resistance, new generations of EGFR TKIs have been developed by targeting newly identified mutations in ... More