Fluorescence lifetime characterization of novel low-pH probes.
AuthorsLin HJ, Herman P, Kang JS, Lakowicz JR
JournalAnal Biochem
PubMed ID11444806
'The structures and functions of the cellular acidic compartments are strongly dependent on the pH gradients across vesicular membranes. Measurement and imaging of the vesicular pH require fluorophores with appropriate pK(a) values. In this report, we characterized the pH-dependent lifetime responses of a family of acidotropic probes, LysoSensors, to evaluate ... More
Illumination of the malaria parasite Plasmodium falciparum alters intracellular pH. Implications for live cell imaging.
AuthorsWissing F, Sanchez CP, Rohrbach P, Ricken S, Lanzer M,
JournalJ Biol Chem
PubMed ID12140286
Live cell fluorescence microscopy has been widely used to study physiological processes in the human malarial parasite Plasmodium falciparum, including pH homeostasis, Ca(2+) signaling and protein targeting. However, the reproducibility of the data is often poor. Controversial statements exist regarding cytosolic and vacuolar baseline pH, as well as regarding the ... More
Kinetics of doxorubicin handling in the LLC-PK1 kidney epithelial cell line is mediated by both vesicle formation and P-glycoprotein drug transport.
AuthorsCrivellato E, Candussio L, Rosati AM, Decorti G, Klugmann FB, Mallardi F
JournalHistochem J
PubMed ID10576412
The subcellular distribution of doxorubicin was evaluated in living non-fixed LLC-PK1 cells, which maintain the structural and functional characteristics of the kidney proximal tubule epithelium and also express P-glycoprotein. After 10 min incubation, doxorubicin fluorescence was detectable in the nucleus. The intensity of nuclear fluorescence progressively increased, reaching the maximum ... More
Quantitative calcium measurements in subcellular compartments of Plasmodium falciparum-infected erythrocytes.
AuthorsRohrbach P, Friedrich O, Hentschel J, Plattner H, Fink RH, Lanzer M
JournalJ Biol Chem
PubMed ID15927958
The acidic food vacuole exerts several important functions during intraerythrocytic development of the human malarial parasite Plasmodium falciparum. Hemoglobin taken up from the host erythrocyte is degraded in the food vacuole, and the heme liberated during this process is crystallized to inert hemozoin. Several anti-malarial drugs target food vacuolar pathways, ... More