Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.
AuthorsCarrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,
JournalJ Immunol
PubMed ID17548620
'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More
High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression.
AuthorsKhalil IA, Kogure K, Futaki S, Harashima H
JournalJ Biol Chem
PubMed ID16326716
'The mechanism of the arginine-rich peptide-mediated cellular uptake is currently a controversial issue. Several factors, including the type of peptide, the nature of the cargo, and the linker between them, appear to affect uptake. One of the less studied factors, which may affect the uptake mechanism, is the effect of ... More
Porphyrin-retinamides: synthesis and cellular studies.
AuthorsSibrian-Vazquez M, Jensen TJ, Vicente MG
JournalBioconjug Chem
PubMed ID17518439
'A series of four porphyrin-retinamides containing either all-trans- or 13-cis-retinoid acid residues, directly linked to the para-phenyl position of meso-tetraphenylporphyrin or via a low-molecular-weight PEG spacer, have been synthesized. The biological properties of these conjugates were evaluated in a model cell line, human HEp2, and in neuroblastoma SK-N-DZ cells, which ... More
Helicobacter pylori enter and survive within multivesicular vacuoles of epithelial cells.
AuthorsAmieva MR, Salama NR, Tompkins LS, Falkow S
JournalCell Microbiol
PubMed ID12366404
'Although intracellular Helicobacter pylori have been described in biopsy specimens and in cultured epithelial cells, the fate of these bacteria is unknown. Using differential interference contrast (DIC) video and immunofluorescence microscopy, we document that a proportion of cell-associated H. pylori enter large cytoplasmic vacuoles, where they remain viable and motile ... More
Carbohydrate oxidation acidifies endosomes, regulating antigen processing and TLR9 signaling.
AuthorsLewis CJ, Cobb BA,
JournalJ Immunol
PubMed ID20200279
'Phagocytes kill encapsulated microbes through oxidative cleavage of surface carbohydrates, releasing glycan fragments and microbial contents that serve as ligands for immune receptors, which tailor the immune response against the offending pathogen. The glycan fragments serve as MHC class II (MHC II) ligands and innate receptor agonists, whereas microbial proteins ... More
Syntaxin 3 is required for cAMP-induced acid secretion: streptolysin O-permeabilized gastric gland model.
AuthorsAmmar DA, Zhou R, Forte JG, Yao X
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID11751154
'Gastric gland stimulation triggers H(+),K(+)-ATPase translocation from cytoplasmic tubulovesicles to apical plasma membrane in parietal cells, resulting in HCl secretion. We studied the mechanisms involved in tubulovesicle translocation with a permeabilized gland system. Streptolysin O (SLO)-treated glands were permeabilized such that exogenous fluorescently labeled actin incorporated into cytoskeleton in a ... More
RabD, a Dictyostelium Rab14-related GTPase, regulates phagocytosis and homotypic phagosome and lysosome fusion.
AuthorsHarris E, Cardelli J
JournalJ Cell Sci
PubMed ID12186956
'RabD, a Dictyostelium Rab14-related GTPase, localizes in the endo-lysosomal pathway and contractile vacuole system of membranes. Cell lines expressing dominant-negative RabD were defective in endocytosis, endosomal membrane flow and homotypic lysosome fusion. In support of a role for RabD in fusion, cells overexpressing constitutively active RabD(Q67L) accumulated enlarged hydrolase-rich acidic ... More
Differential regulation of phagosome maturation in macrophages and dendritic cells mediated by Rho GTPases and ezrin-radixin-moesin (ERM) proteins.
AuthorsErwig LP, McPhilips KA, Wynes MW, Ivetic A, Ridley AJ, Henson PM
JournalProc Natl Acad Sci U S A
PubMed ID16908865
'Deletion of apoptotic cells from tissues involves their phagocytosis by macrophages, dendritic cells, and tissue cells. Although much attention has been focused on the participating ligands, receptors, and mechanisms of uptake, little is known of the disposition of the ingested cell within the phagosome. Here we show that uptake of ... More
A protein containing a serine-rich domain with vesicle fusing properties mediates cell cycle-dependent cytosolic pH regulation.
AuthorsBrazill DT, Caprette DR, Myler HA, Hatton RD, Ammann RR, Lindsey DF, Brock DA, Gomer RH
JournalJ Biol Chem
PubMed ID10747962
'Initial differentiation in Dictyostelium involves both asymmetric cell division and a cell cycle-dependent mechanism. We previously identified a gene, rtoA, which when disrupted randomizes the cell cycle-dependent mechanism without affecting either the underlying cell cycle or asymmetric differentiation. We find that in wild-type cells, RtoA levels vary during the cell ... More
Ca2+ accumulation into acidic organelles mediated by Ca2+- and vacuolar H+-ATPases in human platelets.
AuthorsLópez JJ, Camello-Almaraz C, Pariente JA, Salido GM, Rosado JA
JournalBiochem J
PubMed ID15847604
'Most physiological agonists increase cytosolic free [Ca2+]c (cytosolic free Ca2+ concentration) to regulate a variety of cellular processes. How different stimuli evoke distinct spatiotemporal Ca2+ responses remains unclear, and the presence of separate intracellular Ca2+ stores might be of great functional relevance. Ca2+ accumulation into intracellular compartments mainly depends on ... More
Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization.
AuthorsHornung V, Bauernfeind F, Halle A, Samstad EO, Kono H, Rock KL, Fitzgerald KA, Latz E,
JournalNat Immunol
PubMed ID18604214
'Inhalation of silica crystals causes inflammation in the alveolar space. Prolonged exposure to silica can lead to the development of silicosis, an irreversible, fibrotic pulmonary disease. The mechanisms by which silica and other crystals activate immune cells are not well understood. Here we demonstrate that silica and aluminum salt crystals ... More
Priming of insulin granules for exocytosis by granular Cl(-) uptake and acidification.
AuthorsBarg S, Huang P, Eliasson L, Nelson DJ, Obermüller S, Rorsman P, Thévenod F, Renström E
JournalJ Cell Sci
PubMed ID11493650
'ATP-dependent priming of the secretory granules precedes Ca(2+)-regulated neuroendocrine secretion, but the exact nature of this reaction is not fully established in all secretory cell types. We have further investigated this reaction in the insulin-secreting pancreatic B-cell and demonstrate that granular acidification driven by a V-type H(+)-ATPase in the granular ... More
Characterization of yeast V-ATPase mutants lacking Vph1p or Stv1p and the effect on endocytosis.
AuthorsPerzov N, Padler-Karavani V, Nelson H, Nelson N
JournalJ Exp Biol
PubMed ID11948198
'Subunit a of V-ATPase in the yeast Saccharomyces cerevisiae, in contrast to its other subunits, is encoded by two genes VPH1 and STV1. While disruption of any other gene encoding the V-ATPase subunits results in growth arrest at pH 7.5, null mutants of Vph1p or Stv1p can grow at this ... More
Functional dissection of COP-I subunits in the biogenesis of multivesicular endosomes.
AuthorsGu F, Aniento F, Parton RG, Gruenberg J
JournalJ Cell Biol
PubMed ID9382865
'In the present paper, we show that transport from early to late endosomes is inhibited at the restrictive temperature in a mutant CHO cell line (ldlF) with a ts-defect in epsilon coatomer protein (epsilonCOP), although internalization and recycling continue. Early endosomes then appear like clusters of thin tubules devoid of ... More
Low pH inhibits compensatory endocytosis at a step between depolarization and calcium influx.
AuthorsSmith RM, Baibakov B, Lambert NA, Vogel SS
JournalTraffic
PubMed ID12010458
'Cell function can be modulated by the insertion and removal of ion channels from the cell surface. The mechanism used to keep channels quiescent prior to delivery to the cell surface is not known. In eggs, cortical vesicle exocytosis inserts voltage-gated calcium channels into the cell surface. Calcium influx through ... More
Reactive oxygen species-producing site in radiation and hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID15067365
'In our previous studies, we showed that the apoptotic resistance of the human osteosarcoma cell line HS-Os-1 against irradiation was easily converted to a state of apoptotic-susceptibility by the addition of a relatively low concentration of hydrogen peroxide to the culture medium just prior to irradiation. When we consider the ... More
Using an AraC-based three-hybrid system to detect biocatalysts in vivo.
'Recent methods to create large libraries of proteins have greatly advanced the discovery of proteins with novel functions. However, one limitation in the discovery of new biocatalysts is the screening or selection methods employed to find enzymes from these libraries. We have developed a potentially general method termed QUEST (QUerying ... More
Polycations such as polyethylenimine (PEI) are used in many novel nonviral vector designs and there are continuous efforts to increase our mechanistic understanding of their interactions with cells. Even so, the mechanism of polyplex escape from the endosomal/lysosomal pathway after internalization is still elusive. The ... More
Fluorescent indicators for intracellular pH.
AuthorsHan J, Burgess K,
JournalChem Rev
PubMed ID19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
Monitoring autophagy in lysosomal storage disorders.
AuthorsRaben N, Shea L, Hill V, Plotz P,
JournalMethods Enzymol
PubMed ID19216919
Lysosomes are the final destination of the autophagic pathway. It is in the acidic milieu of the lysosomes that autophagic cargo is metabolized and recycled. One would expect that diseases with primary lysosomal defects would be among the first systems in which autophagy would be studied. In reality, this is ... More
Unimpaired lysosomal acidification in respiratory epithelial cells in cystic fibrosis.
AuthorsHaggie PM, Verkman AS,
JournalJ Biol Chem
PubMed ID19136560
The mechanisms remain uncertain by which mutations in CFTR cause lung disease in cystic fibrosis (CF). Teichgräber et al. recently reported increased ceramide in CF lungs, which was proposed to result from defective lysosomal acidification in airway epithelial cells and consequent impairment of pH-dependent ceramide-metabolizing enzymes (Teichgräber, V., Ulrich, M., ... More
Photoinduced cytotoxicity and biodistribution of prostate cancer cell-targeted porphyrins.
AuthorsSehgal I, Sibrian-Vazquez M, Vicente MG,
JournalJ Med Chem
PubMed ID18839477
A series of five porphyrin-peptide conjugates bearing one or two sequences containing a cell penetrating peptide (CPP), a nuclear localization signal (NLS), or a bifunctional CPP-NLS or NLS-CPP sequences were synthesized and investigated in vitro using PC-3M human prostate cancer cells, in comparison with FDA-approved purified hematoporphyrin derivative (Porfimer Sodium) ... More
Cutting edge: signals from the B lymphocyte antigen receptor regulate MHC class II containing late endosomes.
AuthorsSiemasko K, Eisfelder BJ, Williamson E, Kabak S, Clark MR
JournalJ Immunol
PubMed ID9605114
The B lymphocyte response to protein Ag is dependent upon the successful presentation to T cells of Ag-derived, MHC class II-restricted peptides. The B cell Ag receptor (BCR) facilitates this process by internalizing ligand and delivering it to specialized compartment(s) (MHC class II peptide-loading compartments (MIIC)) where it is processed ... More
Two members of the beige/CHS (BEACH) family are involved at different stages in the organization of the endocytic pathway in Dictyostelium.
AuthorsCornillon S, Dubois A, Brückert F, Lefkir Y, Marchetti A, Benghezal M, De Lozanne A, Letourneur F, Cosson P
JournalJ Cell Sci
PubMed ID11865029
Proteins of the Chediak-Higashi/Beige (BEACH) family have been implicated in the function of lysosomes, as well as in signal transduction, but their molecular role is still poorly understood. In Dictyostelium, at least six members of the family can be identified. Here cells with mutations in two of these genes, LVSA ... More
Glucose metabolism and glutamate analog acutely alkalinize pH of insulin secretory vesicles of pancreatic beta-cells.
AuthorsEto K, Yamashita T, Hirose K, Tsubamoto Y, Ainscow EK, Rutter GA, Kimura S, Noda M, Iino M, Kadowaki T
JournalAm J Physiol Endocrinol Metab
PubMed ID12644449
We studied acute changes of secretory vesicle pH in pancreatic beta-cells with a fluorescent pH indicator, lysosensor green DND-189. Fluorescence was decreased by 0.66 +/- 0.10% at 149 +/- 16 s with 22.2 mM glucose stimulation, indicating that vesicular pH was alkalinized by approximately 0.016 unit. Glucose-responsive pH increase was ... More
A myosin I is involved in membrane recycling from early endosomes.
AuthorsNeuhaus EM, Soldati T
JournalJ Cell Biol
PubMed ID10973992
Geometry-based mechanisms have been proposed to account for the sorting of membranes and fluid phase in the endocytic pathway, yet little is known about the involvement of the actin-myosin cytoskeleton. Here, we demonstrate that Dictyostelium discoideum myosin IB functions in the recycling of plasma membrane components from endosomes back to ... More
Mislocalization of membrane proteins associated with multidrug resistance in cisplatin-resistant cancer cell lines.
AuthorsLiang XJ, Shen DW, Garfield S, Gottesman MM
JournalCancer Res
PubMed ID14522917
The accumulation of [(14)C]carboplatin and [(3)H]methotrexate is reduced in single-step KB epidermoid adenocarcinoma (KB-CP) cells, which are cross-resistant to carboplatin, methotrexate, and sodium arsenite. In these KB-CP cells, multidrug resistance is accompanied by mislocalization of multidrug resistance associated protein (MRP) 1 and other membrane proteins such as folate-binding protein. MRP1 ... More
The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.
AuthorsSalazar G, Love R, Werner E, Doucette MM, Cheng S, Levey A, Faundez V
JournalMol Biol Cell
PubMed ID14657250
Synaptic vesicles (SV) are generated by two different mechanisms, one AP-2 dependent and one AP-3 dependent. It has been uncertain, however, whether these mechanisms generate SV that differ in molecular composition. We explored this hypothesis by analyzing the targeting of ZnT3 and synaptophysin both to PC12 synaptic-like microvesicles (SLMV) as ... More
Reactive oxygen species-producing site in radiation-induced apoptosis of human peripheral T cells: involvement of lysosomal membrane destabilization.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID14654973
In our previous studies, we have partly elucidated the mechanism of radiation-induced apoptosis of human peripheral T cells. The exact site of the ROS (reactive oxygen species) formation induced by irradiation has been so far unknown. Therefore, in this study, we investigated the site of ROS formation by utilizing MitoCapture, ... More
Quantitative three-dimensional analysis of the intracellular trafficking of plasmid DNA transfected by a nonviral gene delivery system using confocal laser scanning microscopy.
AuthorsAkita H, Ito R, Khalil IA, Futaki S, Harashima H
JournalMol Ther
PubMed ID15006612
Since endosomal escape and the nuclear delivery of plasmid DNA (pDNA) constitute major barriers for transgene expression, a quantitative evaluation of intracellular trafficking of pDNA would be highly desirable in terms of optimizing a nonviral gene delivery system. In the present study, a novel strategy is proposed for the quantification ... More
Genome-wide analysis of iron-dependent growth reveals a novel yeast gene required for vacuolar acidification.
We conducted a genome-wide screen in the budding yeast Saccharomyces cerevisiae of 4,792 homozygous diploid deletions to identify genes that function in iron metabolism. Strains unable to grow on iron-restricted medium contained deletions of genes that encode the structural components of the high affinity iron transport system (FET3, FTR1), the ... More
Arbuscules of vesicular-arbuscular mycorrhizal fungi inhabit an acidic compartment within plant roots.
AuthorsGuttenberger M
JournalPlanta
PubMed ID10987547
The most widespread type of mycorrhiza is the so-called vesicular-arbuscular mycorrhiza. In this endomycorrhiza, fungal hyphae penetrate plant cell walls in the root cortex. There they form densely branched arbuscules. Fungus and plant plasma membrane are separated by a common interfacial apoplast. The pH of the compartment between the symbionts ... More
Synaptic vesicle recycling in cultured cerebellar granule cells: role of vesicular acidification and refilling.
AuthorsCousin MA, Nicholls DG
JournalJ Neurochem
PubMed ID9349537
The role of the transvesicular protonmotive force in synaptic vesicle recycling was investigated in cultured cerebellar granule cells. The vesicular V-ATPase was inhibited by 1 microM bafilomycin A1; as an alternative, the pH component of the gradient was selectively collapsed by equilibration of the cells with 10 mM methylamine and ... More
ATP-independent luminal oscillations and release of Ca2+ and H+ from mast cell secretory granules: implications for signal transduction.
AuthorsQuesada I, Chin WC, Verdugo P
JournalBiophys J
PubMed ID12885643
InsP(3) is an important link in the intracellular information network. Previous observations show that activation of InsP(3)-receptor channels on the granular membrane can turn secretory granules into Ca(2+) oscillators that deliver periodic trains of Ca(2+) release to the cytosol (T. Nguyen, W. C. Chin, and P. Verdugo, 1998, Nature, 395:908-912; ... More
Inositol 3,4,5,6-tetrakisphosphate inhibits insulin granule acidification and fusogenic potential.
AuthorsRenström E, Ivarsson R, Shears SB
JournalJ Biol Chem
PubMed ID12055181
ClC Cl(-) channels in endosomes, synaptosomes, lysosomes, and beta-cell insulin granules provide charge neutralization support for the functionally indispensable acidification of the luminal interior by electrogenic H(+)-ATPases (Jentsch, T. J., Stein, V., Weinreich, F., and Zdebik, A. A. (2002) Physiol. Rev. 82, 503-568). Regulation of ClC activity is, therefore, of ... More
Extremely acidophilic protists from acid mine drainage host Rickettsiales-lineage endosymbionts that have intervening sequences in their 16S rRNA genes.
During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this group ... More
Cytoplasmic dynein contains a family of differentially expressed light chains.
AuthorsKing SM, Barbarese E, Dillman JF, Benashski SE, Do KT, Patel-King RS, Pfister KK
JournalBiochemistry
PubMed ID9790665
Cytoplasmic dynein contains a series of accessory proteins associated with the motor containing heavy chains.1 These include three distinct classes of light chains (Mr < approximately 22 000). Here we demonstrate that a previously cloned protein termed rp3 is a bona fide Mr 14 000 light chain component of this ... More
Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: involvement of lysosomal membrane destabilization.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID14767567
In our previous study, we examined the effect of exogenous hydrogen peroxide, which causes a potent oxidative stress and has been demonstrated to be a potent apoptosis-inducer in many kinds of cells. We found that the addition of 1 or 10 mM hydrogen peroxide induced reactive oxygen species (ROS) formation, ... More
Dictyostelium LvsB mutants model the lysosomal defects associated with Chediak-Higashi syndrome.
AuthorsHarris E, Wang N, Wu Wl WL, Weatherford A, De Lozanne A, Cardelli J
JournalMol Biol Cell
PubMed ID11854420
Chediak-Higashi syndrome is a genetic disorder caused by mutations in a gene encoding a protein named LYST in humans ("lysosomal trafficking regulator") or Beige in mice. A prominent feature of this disease is the accumulation of enlarged lysosome-related granules in a variety of cells. The genome of Dictyostelium discoideum contains ... More
SUR1 regulates PKA-independent cAMP-induced granule priming in mouse pancreatic B-cells.
AuthorsEliasson L, Ma X, Renström E, Barg S, Berggren PO, Galvanovskis J, Gromada J, Jing X, Lundquist I, Salehi A, Sewing S, Rorsman P
JournalJ Gen Physiol
PubMed ID12601083
Measurements of membrane capacitance were applied to dissect the cellular mechanisms underlying PKA-dependent and -independent stimulation of insulin secretion by cyclic AMP. Whereas the PKA-independent (Rp-cAMPS-insensitive) component correlated with a rapid increase in membrane capacitance of approximately 80 fF that plateaued within approximately 200 ms, the PKA-dependent component became prominent ... More
The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia.
Authors Touz Maria C.; Nores Maria J.; Slavin Ileana; Carmona Carlos; Conrad John T.; Mowatt Michael R.; Nash Theodore E.; Coronel Carlos E.; Lujan Hugo D.;
JournalJ Biol Chem
PubMed ID11773053
Giardia is an intestinal parasite that belongs to the earliest diverging branch of the eukaryotic lineage of descent. Giardia undergoes adaptation for survival outside the host's intestine by differentiating into infective cysts. Encystation involves the synthesis and transport of cyst wall constituents to the plasma membrane for release and extracellular ... More
Impaired platelet activation in familial high density lipoprotein deficiency (Tangier disease).
AuthorsNofer JR, Herminghaus G, Brodde M, Morgenstern E, Rust S, Engel T, Seedorf U, Assmann G, Bluethmann H, Kehrel BE
JournalJ Biol Chem
PubMed ID15163665
ATP binding cassette transporter A1 (ABCA1) is involved in regulation of intracellular lipid trafficking and export of cholesterol from cells to high density lipoproteins. ABCA1 defects cause Tangier disease, a disorder characterized by absence of high density lipoprotein and thrombocytopenia. In the present study we have demonstrated that ABCA1 is ... More
Intracellular trafficking of Brucella abortus in J774 macrophages.
AuthorsArenas GN, Staskevich AS, Aballay A, Mayorga LS
JournalInfect Immun
PubMed ID10858243
Brucella abortus is a facultative intracellular bacterium capable of surviving inside professional and nonprofessional phagocytes. The microorganism remains in membrane-bound compartments that in several cell types resemble modified endoplasmic reticulum structures. To monitor the intracellular transport of B. abortus in macrophages, the kinetics of fusion of phagosomes with preformed lysosomes ... More
Benzyl-N-acetyl-alpha-D-galactosaminide induces a storage disease-like phenotype by perturbing the endocytic pathway.
AuthorsUlloa F, Real FX
JournalJ Biol Chem
PubMed ID12538583
The sugar analog O-benzyl-N-acetyl-alpha-d-galactosaminide (BG) is an inhibitor of glycan chain elongation and inhibits alpha2,3-sialylation in mucus-secreting HT-29 cells. Long-term exposure of these cells to BG is associated with the accumulation of apical glycoproteins in cytoplasmic vesicles. The mechanisms involved therein and the nature of the vesicles have not been ... More
ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive aberrant growth signalling in Niemann-Pick type C.
AuthorsLim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL, Ory DS, Nomura DK, Zoncu R
JournalNat Cell Biol
PubMed ID31548609
'Cholesterol activates the master growth regulator, mTORC1 kinase, by promoting its recruitment to the surface of lysosomes by the Rag guanosine triphosphatases (GTPases). The mechanisms that regulate lysosomal cholesterol content to enable mTORC1 signalling are unknown. Here, we show that oxysterol binding protein (OSBP) and its anchors at the endoplasmic ... More
Efferocytosis requires periphagosomal Ca2+-signaling and TRPM7-mediated electrical activity.
Authors
JournalNat Commun
PubMed ID35680919
Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.
Authors
JournalJ Clin Invest
PubMed ID29920188
Decoy exosomes provide protection against bacterial toxins.
AuthorsKeller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K
JournalNature
PubMed ID32132711
'The production of pore-forming toxins that disrupt the plasma membrane of host cells is a common virulence strategy for bacterial pathogens such as methicillin-resistant Staphylococcus aureus (MRSA)' ... More
ER proteins decipher the tubulin code to regulate organelle distribution.
Authors
JournalNature
PubMed ID34912111
Design of efficacious somatic cell genome editing strategies for recessive and polygenic diseases.
Authors
JournalNat Commun
PubMed ID33293555
An intestinal zinc sensor regulates food intake and developmental growth.
Authors
JournalNature
PubMed ID32269334
Diclofenac impairs autophagic flux via oxidative stress and lysosomal dysfunction: Implications for hepatotoxicity.
B lymphocytes capture antigens from the surface of presenting cells by forming an immune synapse. Local secretion of lysosomes, which are guided to the synaptic membrane by centrosome repositioning, can facilitate the extraction of immobilized antigens. However, the molecular basis underlying their delivery to precise domains of the plasma membrane ... More
Autophagic flux disruption contributes to Ganoderma lucidum polysaccharide-induced apoptosis in human colorectal cancer cells via MAPK/ERK activation.
AuthorsPan H, Wang Y, Na K, Wang Y, Wang L, Li Z, Guo C, Guo D, Wang X
JournalCell Death Dis
PubMed ID31186406
Targeting autophagy may serve as a promising strategy for cancer therapy. Ganoderma lucidum polysaccharide (GLP) has been shown to exert promising anti-cancer effects. However, the underlying mechanisms remain elusive. Whether GLP regulates autophagy in cancer has never been reported. In this study, GLP induced the initiation of autophagy in colorectal ... More
Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK.
AuthorsLi M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou Z, Xie XS, Hardie DG, Lin SC
JournalCell Metab
PubMed ID31204282
Fructose-1,6-bisphosphate (FBP) aldolase links sensing of declining glucose availability to AMPK activation via the lysosomal pathway. However, how aldolase transmits lack of occupancy by FBP to AMPK activation remains unclear. Here, we show that FBP-unoccupied aldolase interacts with and inhibits endoplasmic reticulum (ER)-localized transient receptor potential channel subfamily V, inhibiting ... More
Shift in Immune Parameters After Repeated Exposure to Nanoplastics in the Marine Bivalve
AuthorsAuguste M, Balbi T, Ciacci C, Canonico B, Papa S, Borello A, Vezzulli L, Canesi L
JournalFront Immunol
PubMed ID32351496
Bivalves are widespread in coastal environments subjected to a wide range of environmental fluctuations: however, the rapidly occurring changes due to several anthropogenic factors can represent a significant threat to bivalve immunity. The mussel ... More
Topology, Antiviral Functional Residues and Mechanism of IFITM1.
AuthorsSun F, Xia Z, Han Y, Gao M, Wang L, Wu Y, Sabatier JM, Miao L, Cao Z
JournalViruses
PubMed ID32182730
Interferon-inducible transmembrane proteins (IFITM1/2/3) have been reported to suppress the entry of a wide range of viruses. However, their antiviral functional residues and specific mechanisms are still unclear. Here, we firstly resolved the topology of IFITM1 on the plasma membrane where N-terminus points into the cytoplasm and C-terminus resides extracellularly. ... More