The release of oxygen radicals by blood and bone marrow leukocytes of patients with Fanconi anemia (FA) has been studied. It was found that the nonstimulated FA leukocytes and those stimulated by concanavalin A, SiO2, latex, and opsonized zymosan produced enhanced levels of luminol- and lucigenin-dependent chemiluminescence (CL) in comparison ... More
Interaction of Mycobacterium avium-containing phagosomes with the antigen presentation pathway.
AuthorsUllrich HJ, Beatty WL, Russell DG
JournalJ Immunol
PubMed ID11086039
'Pathogenic mycobacteria infect macrophages where they replicate in phagosomes that minimize contact with late endosomal/lysosomal compartments. Loading of Ags to MHC class II molecules occurs in specialized compartments with late endosomal characteristics. This points to a sequestration of mycobacteria-containing phagosomes from the sites where Ags meet MHC class II molecules. ... More
Determination of phospholipid hydroperoxides using luminol chemiluminescence--high-performance liquid chromatography.
AuthorsMiyazawa T, Fujimoto K, Suzuki T, Yasuda K
JournalMethods Enzymol
PubMed ID8015467
Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils.
AuthorsLucas M, Solano F
JournalAnal Biochem
PubMed ID1332545
'The oxidation of free coelenterazine by superoxide anion was analyzed and compared to the oxidation by the semisynthetic photoprotein obelin, prepared by incorporation of synthetic coelenterazine into apoobelin. The oxidation of bound coelenterazine was triggered upon binding of calcium to the reconstituted photoprotein. The oxidation of free synthetic coelenterazine, in ... More
Antioxidant activity of micronized diosmin on oxygen species from stimulated human neutrophils.
AuthorsCypriani B, Limasset B, Carrié ML, Le Doucen C, Roussie M, de Paulet AC, Damon M
JournalBiochem Pharmacol
PubMed ID8385947
'Flavonoids are known to reduce reactive oxygen species released by polymorphonuclear neutrophils (PMNs) in vitro. We have studied the effects of S5682 (Daflon 500 mg), a purified flavonoid fraction composed of 90% diosmin and 10% hesperidin. S5682 produced a dose-dependent inhibition of the luminol chemiluminescence (CL) induced by phorbol myristate ... More
Effect of Polyoxidonium on the Phagocytic Activity of Human Peripheral Blood Leukocytes.
'The effect of polyoxidonium on the functional activity of human peripheral blood phagocytes was studied in vitro. Polyoxidonium is an N-oxidized polyethylene-piperazine derivative, a water-soluble high-molecular synthetic immunomodulator. It was established that a one-hour incubation of leukocytes with polyoxidonium increases the ability of leukocytes to kill the ingested Staphylococcus aureus ... More
Relationship between resazurin reduction test, reactive oxygen species generation, and gamma-glutamyltransferase.
AuthorsZalata A, Hafez T, Mahmoud A, Comhaire F
JournalHum Reprod
PubMed ID7657753
'The production of reactive oxygen species (ROS) from spermatozoa has been measured by chemiluminescence in the two fractions of a Percoll gradient column (47 and 90%). Chemiluminescent signals were recorded in each fraction after addition of luminol and horse-radish peroxidase (basal state), and after stimulation with formyl-methionyl-leucyl-phenyl-alanine (FMLP) and phorbol ... More
Ku entry into DNA inhibits inward DNA transactions in vitro.
AuthorsFrit P, Li RY, Arzel D, Salles B, Calsou P
JournalJ Biol Chem
PubMed ID10945984
'Association of the DNA end-binding Ku70/Ku80 heterodimer with the 460-kDa serine/threonine kinase catalytic subunit forms the DNA-dependent protein kinase (DNA-PK) that is required for double-strand break repair by non-homologous recombination in mammalian cells. Recently, we have proposed a model in which the kinase activity is required for translocation of the ... More
Evidence for superoxide dismutase and catalase in mollicutes and release of reactive oxygen species.
AuthorsMeier B, Habermehl GG
JournalArch Biochem Biophys
PubMed ID2154957
'The presence of superoxide dismutase was demonstrated in 21 strains of mollicutes, including achuloplasmas, mycoplasmas, and ureaplasmas. No superoxide dismutase activities or only traces were detectable in fresh prepared cell lysates, whereas activities were evident after dialysis of the cell lysates. A further increase in superoxide dismutase activities was observed ... More
Development of novel high-sensitivity chemiluminescence assay for luminol using thiourea derivatives.
AuthorsOhno K, Arakawa H, Yoda R, Maeda M
JournalLuminescence
PubMed ID10602308
'We have screened about 100 thiourea derivatives in order to develop a sensitive chemiluminescence detection for luminol derivatives. Among these derivatives, we found a new compound, 2-(3-methylthioureido) thiazole, that could be used to measure luminol in the presence of hydrogen peroxide (H(2)O(2)). The detection limits of luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) ... More
In vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor.
AuthorsSuematsu M, Kurose I, Asako H, Miura S, Tsuchiya M
JournalJ Biochem (Tokyo)
PubMed ID2808326
'Oxyradical-dependent chemiluminescence from granulocytes sticking to venular endothelium was successfully visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF). Intravital photonic image intensifier microscopy revealed that topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on endothelial walls and the subsequent activation of a luminol-dependent photonic burst. ... More
Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore--role of degranulation and myeloperoxidase.
AuthorsSuematsu M, Oshio C, Miura S, Suzuki M, Houzawa S, Tsuchiya M
JournalBiochem Biophys Res Commun
PubMed ID2843175
'Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 microgram/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 micrograms/ml). The additional application of 0.1 microM Ca2+ ionophore ... More
Imaging and detection technologies for image analysis in electrophoresis.
AuthorsMiura K
JournalElectrophoresis
PubMed ID11332747
'Image capture is the first step of image analysis. There are two major devices for image capture in the field of electrophoresis. One is the charged-couple device (CCD) camera and the other is the scanner. Image capture technologies have shown great progress in recent years especially in the field of ... More
An amplified chemiluminescent assay for the detection of alkaline phosphatase.
AuthorsFisher M, Harbron S, Rabin BR
JournalAnal Biochem
PubMed ID7668394
'An amplification assay for the measurement of alkaline phosphatase has been combined with a luminescent end point using the luminol-peroxidase system to produce the first enzyme-amplified chemiluminescent assay based on the principle of prosthetogenesis. This assay is both quantitative and extremely sensitive. When the assay was used to detect alkaline ... More
Chemiluminescence detection of H2O2 produced by human neutrophils during the respiratory burst.
AuthorsWymann MP, von Tscharner V, Deranleau DA, Baggiolini M
JournalAnal Biochem
PubMed ID3425906
'A sensitive luminol-dependent chemiluminescence assay for H2O2 was developed for the indirect determination of the transient changes in NADPH oxidase activity associated with the respiratory burst of human neutrophils. A relatively large, controlled amount of horseradish peroxidase was used in combination with added luminol to rapidly remove and simultaneously detect ... More
Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors.
'Cells isolated from the ejaculates of a high proportion of patients exhibiting oligozoospermia are characterized by generation rates of reactive oxygen species that considerably exceed those obtained for the normal fertile population. The purpose of this study was to resolve the cellular source of this enhanced activity. Semen samples from ... More
Oxygenation activities of chicken polymorphonuclear leukocytes investigated by selective chemiluminigenic probes.
AuthorsMerrill GA, Bretthauer R, Wright-Hicks J, Allen RC
JournalLab Anim Sci
PubMed ID8905586
'The redox metabolism of myeloperoxidase-deficient rooster (chicken) polymorphonuclear leukocytes (PMNL) was analyzed by differential chemiluminigenic probes. Chicken complement-opsonified zymosan, a phagocytosable particulate stimulus, and phorbol myristate acetate, a chemical stimulus, were used to activate the PMNL respiratory burst. The two probes used were luminol (5-amino-2,3-dihydro-1,4-phthalazinedione), a general probe of oxidase-peroxidase ... More
Induction of luminol chemiluminescence by the manganese cluster of the photosystem II water-oxidizing complex to the S0, S1, S2, and S3 states.
AuthorsPutrenko II
JournalBiochemistry
PubMed ID8608122
'Luminol chemiluminescence (CL) (lambda(max) = 425 nm) induced by the manganese cluster of photosystem II (PSII) (t(max) = 1-5 min) along with CL induced by mono-, di-, or tetranuclear manganese coordination complexes (t(max) = 2-40 s) is observed when either 200 mM sodium phosphate or 1 M Tris-HCl + 200 ... More
Autoxidation of dopamine: a comparison of luminescent and spectrophotometric detection in basic solutions.
AuthorsKlegeris A, Korkina LG, Greenfield SA
JournalFree Radic Biol Med
PubMed ID7744304
'Oxidation products of catecholamines, particularly dopamine, could play an important role in the physiology and pathology of the nervous system. This study has therefore characterised autoxidation of dopamine monitored in a variety of systems. Lucigenin-dependent chemiluminescence and reduction of cytochrome c were exploited to register generation of the byproduct superoxide ... More
Influence of oestrogens on formation of reactive oxygen species in liver microsomes of differently aged male Wistar rats.
AuthorsBarth A, Landmann G, Liepold K, Zapf H, Müller D, Karge E, Klinger W
JournalExp Toxicol Pathol
PubMed ID10445383
'Metabolic pathways of oestrogens are the formation of catechol oestrogens (CE; 2- and 4-hydroxy-oestrogens), redox cycling of CE and free radical generation, mediated through cytochrome P450 (P450) oxidase/reductase activity. We checked the oestrogens oestradiol (E2), oestradiol valerate (E2V) and ethinyloestradiol (EE2) for formation of reactive oxygen species in vitro and ... More
Resazurin reduction as a function of respiratory burst in bovine neutrophils.
AuthorsFang W, Myllys V, Sandholm M
JournalAm J Vet Res
PubMed ID9185965
'OBJECTIVE: To determine whether the respiratory burst of neutrophils from bovine blood and milk can be analyzed by use of a fluorometric resazurin reduction assay. SAMPLE POPULATION: Neutrophils were obtained from EDTA-anticoagulated blood of 7 dairy cows. Neutrophils also were isolated from milk samples of a cow intramammarily challenge exposed ... More
Comparative in vitro investigations of the influence of mofebutazone, phenylbutazone and diclofenac on phagocytosis and respiratory burst of human peripheral blood leucocytes.
AuthorsNeumüller J, Tohidast-Akrad M
JournalArzneimittelforschung
PubMed ID8024638
'The phagocytosis and the release of oxidative products generated by the respiratory burst have been studied under the influence of the non-steroidal anti-inflammatories (NSAID) phenylbutazone (PB), mofebutazone (monophenylbutazone, MPB) and diclofenac (DF) using phagocytes of the peripheral blood from healthy human donors and patients with soft tissue rheumatism. The measurement ... More
Platelet-activating factor and granulocyte-mediated oxidative stress. Strategy for in vivo oxyradical visualization.
AuthorsSuematsu M, Tsuchiya M
JournalLipids
PubMed ID1668122
'Platelet-activating factor (PAF) and leukotriene B4 are potent lipid mediators of endothelium-granulocyte interaction which results in granulocyte-dependent increased microvascular permeability. The specific mechanism by which PAF induces granulocyte-mediated endothelial injury has not been fully investigated. Digital imaging photonic intensified microscopy has revealed that PAF effectively induces granulocyte-mediated oxidative stress on ... More
Further characterization of the neutrophil oxidative burst by flow cytometry.
AuthorsSmith JA, Weidemann MJ
JournalJ Immunol Methods
PubMed ID8315293
'The ability to generate reactive oxygen species is essential for neutrophils to kill infectious micro-organisms. We have investigated the ''oxidative burst'' in human neutrophils stimulated in vitro with either opsonized zymosan (OZ) or phorbol myristate acetate (PMA) at the single cell level by flow cytometry using dichlorofluorescein diacetate (DCFH-DA) and ... More
Investigation on possible antioxidative properties of the NMDA-receptor antagonists ketamine, memantine, and amantadine in comparison to nicanartine in vitro.
AuthorsLupp A, Kerst S, Karge E, Quack G, Klinger W
JournalExp Toxicol Pathol
PubMed ID9784030
'Possible antioxidative properties of three N-methyl-D-aspartate (NMDA)-receptor antagonists, the anesthetic ketamine and the antiparkinson drugs memantine and amantadine were investigated in vitro on the microsomal cytochrome P450 (P450) system of rat livers and on rat whole blood chemiluminescence in comparison to nicanartine, a substance with known antiatherosclerotic, hypolipemic and antioxidative ... More
Release of reactive oxygen by hepatocytes on reoxygenation: three phases and role of mitochondria.
AuthorsLittauer A, de Groot H
JournalAm J Physiol
PubMed ID1616032
'Reoxygenation of isolated hepatocytes in primary culture resulted in a three-phase response in the release of reactive oxygen species (ROS) as determined by peroxidase-dependent luminol chemiluminescence. Release of ROS within the first and second phase correlated well with the extent of reoxygenation injury, both being most significant after approximately 4 ... More
Effects of neopterin-derivatives on H2O2-induced luminol chemiluminescence: mechanistic aspects.
AuthorsMurr C, Baier-Bitterlich G, Fuchs D, Werner ER, Esterbauer H, Pfleiderer W, Wachter H
JournalFree Radic Biol Med
PubMed ID8886794
'Neopterin, 6-D-erythro-1'',2'',3''-trihydroxypropyl-pterin, and its dihydroform, 7,8-dihydro-neopterin, are synthesized by human monocytes/macrophages upon stimulation by interferon-gamma. In the presence of iron chelator complexes neopterin enhances hydrogen peroxide-induced luminol chemiluminescence at neutral or slightly alkaline pH (7.5). In contrast, 7,8-dihydroneopterin scavenges chemiluminescence independently from the pH value and iron. In this study, ... More
Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury.
AuthorsWang P, Zweier JL
JournalJ Biol Chem
PubMed ID8910581
'Altered nitric oxide (NO.) production is a critical factor in tissue reperfusion injury; however, controversy remains regarding these alterations and how they cause injury. Since superoxide (O-2) generation is triggered during the early period of reperfusion the cytotoxic oxidant peroxynitrite (ONOO-) could be formed, but it is not known if ... More
Nonstructural 3 protein of hepatitis C virus triggers an oxidative burst in human monocytes via activation of NADPH oxidase.
AuthorsBureau C, Bernad J, Chaouche N, Orfila C, Béraud M, Gonindard C, Alric L, Vinel JP, Pipy B
JournalJ Biol Chem
PubMed ID11304537
'It has been shown that oxidative stress occurs in chronic hepatitis C. Release of reactive oxygen species (ROS) from sequestered phagocytes and activated resident macrophages represents the predominant component of oxidative stress in the liver. However, little is known about the ability of the monocyte to produce ROS in response ... More
Respiratory burst in human neutrophils.
AuthorsDahlgren C, Karlsson A
JournalJ Immunol Methods
PubMed ID10618505
'During phagocytosis of microbial intruders, professional phagocytes of our innate immune system increase their oxygen consumption through the activity of an NADPH-oxidase that generates superoxide anion (O(2)(-)) and hydrogen peroxide (H(2)O(2)). These oxygen metabolites give rise to yet other reactive oxygen species that are strongly anti-microbial but which may also ... More
Simultaneous measurement of O2 radicals and pulmonary vascular reactivity in rat lung.
AuthorsArcher SL, Nelson DP, Weir EK
JournalJ Appl Physiol
PubMed ID2532193
'The role of endogenous radicals in the regulation of pulmonary vascular tone was evaluated by simultaneous measurement of pulmonary artery pressure and lung radical levels during exposure of isolated rat lungs to varying inspired O2 concentrations (0-95%) and angiotensin II. Lung radical levels, measured "on-line" using luminol and lucigenin-enhanced chemiluminescence, ... More
Contribution of nitric oxide synthase to luminol-dependent chemiluminescence generated by phorbol-ester-activated Kupffer cells.
AuthorsWang JF, Komarov P, Sies H, de Groot H
JournalBiochem J
PubMed ID1718262
'Phorbol 12-myristate 13-acetate-induced luminol chemiluminescence in rat Kupffer cells was doubled by the addition of L-arginine and significantly (up to 70%) inhibited by NG-nitro-L-arginine and NG-monomethyl-L-arginine, competitive inhibitors of L-arginine-dependent nitric oxide (NO) formation. The release of superoxide anion (O2-) by NADPH oxidase was neither affected by L-arginine nor by ... More
Chemiluminescence microscopy as a tool in biomedical research.
AuthorsCréton R, Jaffe LF
JournalBiotechniques
PubMed ID11730016
'Chemiluminescence has become a standard tool in biomedical research. Chemiluminescent probes are used for immunoassays, nucleic acid identification, reporter gene assays, measuring enzyme activity, and the detection of ions and small molecules such as Ca2+, ATP, NO, O2- and H2O2. Along with the development of new chemiluminescent probes, significant progress ... More
Effect of linear polarized near-infrared ray irradiation on the chemiluminescence of human neutrophils and serum opsonic activity.
AuthorsShiraishi M, Suzuki K, Nakaji S, Sugawara K, Sugita N, Suzuki KJ, Ohta S
JournalLuminescence
PubMed ID10512987
'The purpose of this study was to investigate the in vitro effects of linear polarized near-infrared ray irradiation on neutrophil chemiluminescence (CL) and serum opsonic activity. We used luminol- and lucigenin-dependent CL to detect the affected reactive oxygen species production process of human neutrophils and measured serum opsonic activity based ... More
Luminol-dependent chemiluminescence microassay for phagocytic function.
'A luminol-dependent chemiluminescence (CL) microassay was developed to measure phagocytic function of peripheral blood leukocytes. Buffy coats, obtained by centrifugation of only 100 microliter of whole blood, provided an enriched population of polymorphonuclear leukocytes (PMNs). The total reaction mixture, consisting of leukocytes-luminol-inducer (opsonized zymosan), was 450 microliter. Peak CL activity ... More
Effects of human serum on bacterial competition with neutrophils for molecular oxygen.
AuthorsBritigan BE, Cohen MS
JournalInfect Immun
PubMed ID3086230
'A dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. Its effect on other human pathogens was investigated. A 10% serum solution increased peak O2 consumption for Escherichia coli and Staphylococcus aureus to 157% (P less than 0.05) and 199% (P less than 0.02), respectively, of their ... More
Fluorescent liposomes as quantitative markers of phagocytosis by alveolar macrophages.
AuthorsPerry DG, Martin WJ
JournalJ Immunol Methods
PubMed ID7538159
'A new phagocytic assay based on liposome ingestion by alveolar macrophages (AMs) is described. Fluorescent microspheres were encapsulated in liposomes, which allowed rapid enumeration by fluorometry. Liposomes made in the presence of vitronectin had the protein exposed on their outer surfaces, as determined by immunolabelling. Liposomes and rat AMs were ... More
Age-related accumulation of phosphatidylcholine hydroperoxide in cultured human diploid cells and its prevention by alpha-tocopherol.
AuthorsSuzuki T, Miyazawa T, Fujimoto K, Otsuka M, Tsutsumi M
JournalLipids
PubMed ID8377593
'The levels of phosphatidylcholine hydroperoxide in serially cultured human fetal diploid fibroblasts at various population doubling levels were determined by high-performance liquid chromatography combined with chemiluminescence detection. This methodology utilizes a mixture of cytochrome c and luminol as post-column hydroperoxide group specific luminescent reagents. The cellular hydroperoxide content increased with ... More
Photocatalytic and free radical interactions of the heterocyclic N-oxide resazurin with NADH, GSH, and Dopa.
AuthorsPrütz WA, Butler J, Land EJ
JournalArch Biochem Biophys
PubMed ID8619609
'Electron donating free radicals NAD(.), (.)CO2(-), MV(.)+, and e(aq)-, generated by pulse radiolysis, reduce resazurin (RNO) with rate constants of 1.9 x 10(9), 2.8 x 10(9), 4.8 x 10(9), and 2.3 x 10(10) M(-1) s(-1), respectively, neutral solution. The semireduced dye (RN(.)-O- disproportionates slowly to RN (resorufin) and RNO. There ... More
Reactive oxygen species associated with cell differentiation in Neurospora crassa.
AuthorsHansberg W, de Groot H, Sies H
JournalFree Radic Biol Med
PubMed ID8458586
'The conidiation process of Neurospora crassa is characterized by three morphogenetic events: adhesion of hyphae, development of aerial hyphae, and conidia formation. At the onset of all three events a spontaneous, low-level chemiluminescence was detected, indicating the formation of reactive oxygen species. Hyperoxic conditions increased chemiluminescence and accelerated differentiation. Hypoxic ... More
'The mechanism of luminol chemiluminescence is a special case of nucleophilic addition to carbonyl compounds. The breakdown of the key intermediate, an alpha hydroxy hydroperoxide, produces a peracid ortho to an acyl diazene group. After intramolecular addition of the peracid, the energy from nitrogen expulsion is utilized in the formation ... More
Luminescent visualization of low amounts of cytochrome P450 and hemoproteins by luminol in acrylamide gels.
AuthorsBonfils C, Charasse S, Bonfils JP, Larroque C
JournalAnal Biochem
PubMed ID7793632
'In this paper we apply luminol (5-amino-2,3-dihydro-1,4-phthalazinedione), a light-emitting substrate, in conjunction with H2O2 to the luminescence labeling of hemoproteins. We describe in detail a photodetection device which permits an efficient recording of the light emitted by heme-containing proteins resolved in acrylamide gels. The sensitivity of this procedure, when compared ... More
Role of high-frequency ventilation in surfactant-depleted lung injury as measured by granulocytes.
AuthorsMatsuoka T, Kawano T, Miyasaka K
JournalJ Appl Physiol
PubMed ID8175560
'Granulocytes were collected from the lung lavage fluid of surfactant-depleted rabbits to assess the relationship between granulocyte-related lung injury and ventilatory mode. The number of lavaged granulocytes was determined after 2 and 4 h of conventional mechanical ventilation (2CMV and 4CMV, respectively) or high-frequency oscillatory ventilation (2HFO and 4HFO, respectively). ... More
A myeloperoxidase-specific assay based upon bromide-dependent chemiluminescence of luminol.
AuthorsHaqqani AS, Sandhu JK, Birnboim HC
JournalAnal Biochem
PubMed ID10452808
'Measurement of myeloperoxidase (MPO; EC 1.11.1.7) activity is often used as a marker of neutrophil infiltration into tissues. However, most enzymatic assays for MPO are susceptible to interference from other peroxidases (including eosinophil peroxidase, EPX) and hemoproteins (such as hemoglobin and myoglobin) present in the tissues. In this report, we ... More
On the action of hypochlorite on human serum albumin.
AuthorsArnhold J, Hammerschmidt S, Wagner M, Mueller S, Arnold K, Grimm E
JournalBiomed Biochim Acta
PubMed ID1964376
'The luminol-enhanced chemiluminescence of zymosan-stimulated polymorphonuclear leukocytes is continuously diminished in the presence of increasing amounts of human serum albumin (from 1 to 30 mg/ml). HSA competes with luminol for hypochlorite as shown by adding sodium hypochlorite to luminol solutions containing HSA. Titration of HSA with NaOCl affects firstly the ... More
Antioxidative activity of flavonoids in stimulated human neutrophils.
AuthorsZielinska M, Kostrzewa A, Ignatowicz E
JournalFolia Histochem Cytobiol
PubMed ID10763121
'The release and production of oxidative products generated by the respiratory burst under the influence of natural flavonoids: quercetin, kaempferol and isorhamnetin derivatives have been studied in the polymorphonuclear neutrophils (PMNs) from healthy human donors. Flavonoids were tested in vitro at concentration range 1-100 microM. The antioxidative potential of flavonoids ... More
Anticonvulsive and free radical scavenging activities of vanillyl alcohol in ferric chloride-induced epileptic seizures in Sprague-Dawley rats.
AuthorsHsieh CL, Chang CH, Chiang SY, Li TC, Tang NY, Pon CZ, Hsieh CT, Lin JG
JournalLife Sci
PubMed ID10954052
'Vanillyl alcohol (VA) is a component of Gastrodia elata Bl. (GE), which is a traditional Chinese herb widely used to treat convulsive disorders or dizziness. This study examined the role of VA in the anticonvulsive properties of GE in a Sprague-Dawley rat model of epilepsy. The anticonvulsive and free radical ... More
The effect of three human recombinant hematopoietic growth factors (granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, and interleukin-3) on phagocyte oxidative activity.
AuthorsSullivan GW, Carper HT, Mandell GL
JournalBlood
PubMed ID7681704
'Hematopoietic growth factors not only modulate blood progenitor cell activity but also alter the function of mature phagocytes. Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF; 1 ng/mL for 60 min) did not stimulate luminol-enhanced chemiluminescence of polymorphonuclear leukocytes (PMNs) in suspension but primed PMN for as much as a 15-fold increase ... More
Chemiluminescence detection for hybridization assays on the flow-thru chip, a three-dimensional microchannel biochip.
AuthorsCheek BJ, Steel AB, Torres MP, Yu YY, Yang H
JournalAnal Chem
PubMed ID11791544
'Chemiluminescence (CL) detection is seldom used in two-dimensional solid support microarray platforms because adequate sensitivity and spatial resolution is difficult to achieve. The three-dimensional ordered microchannels of the Flow-thru Chip increase both the sensitivity and spatial resolution required for quantitative CL measurements on microarrays. Enzyme-catalyzed CL reactions for the detection ... More
Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay.
AuthorsMiyazawa T
JournalFree Radic Biol Med
PubMed ID2806945
'A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was developed (Miyazawa, T. et al., Anal. Lett., 20, 915-925, 1987) and applied for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in biological tissues such as human blood plasma (Miyazawa, T. et al., Anal Lett 21:1033-1044, 1988; J. Biochem. 103:744-746; 1988). This system ... More
High-sensitive chemiluminescent assay for cholesterol.
AuthorsChamoin MC, Charbonnier M, Lafont H, Ternaux JP
JournalBiochim Biophys Acta
PubMed ID8280764
'Chemiluminescent measurement of cholesterol can be performed in various biological tissues and fluids. The method described in this study has a sensitivity of 54 pmol. The tissue samples used for the determination of cholesterol can be reduced to as little as 1 mg and assay can be performed on diluted ... More
Biotin in vitro translation, nonradioactive detection of cell-free synthesized proteins.
AuthorsHoeltke HJ, Ettl I, Strobel E, Leying H, Zimmermann M, Zimmermann R
JournalBiotechniques
PubMed ID7619497
'In vitro translation of mRNAs into proteins is frequently used to study the coding capacity of RNAs or cDNAs and the functional effects of mutations. In vitro translation assays have traditionally been monitored by following the incorporation of a radiolabeled amino acid into newly synthesized protein. We have optimized an ... More
Helicobacter pylori-associated ammonia production enhances neutrophil-dependent gastric mucosal cell injury.
AuthorsSuzuki M, Miura S, Suematsu M, Fukumura D, Kurose I, Suzuki H, Kai A, Kudoh Y, Ohashi M, Tsuchiya M
JournalAm J Physiol
PubMed ID1443147
'The role of neutrophil and its chlorinated oxidant were investigated in Helicobacter pylori-induced gastric mucosal injury in vitro. Luminol-dependent chemiluminescence (ChL) was used to detect neutrophil-derived oxidants. ChL activity was significantly elevated when neutrophils were incubated in H. pylori, indicating that H. pylori actually elicits oxidative burst of neutrophils. To ... More
Chemiluminometric sensor for simultaneous determination of L-glutamate and L-lysine with immobilized oxidases in a flow injection system.
AuthorsKiba N, Miwa T, Tachibana M, Tani K, Koizumi H
JournalAnal Chem
PubMed ID11922293
'A chemiluminometric flow-through sensor for simultaneous determination of L-glutamate (Glu) and L-lysine (Lys) in a single sample has been developed. Immobilized uricase, immobilized peroxidase, support material, coimmobilized glutamate oxidase/peroxidase, support material, and coimmobilized lysine oxidase/peroxidase were packed sequentially in a transparent PTFE tube, and the tube was placed in front ... More
Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012.
AuthorsDaiber A, August M, Baldus S, Wendt M, Oelze M, Sydow K, Kleschyov AL, Munzel T
JournalFree Radic Biol Med
PubMed ID14732294
'In the present study we sought to determine the ability of the chemiluminescence dye 8-amino-5-chloro-7-phenylpyridol[3,4-d]pyridazine-1,4-(2H,3H)dione sodium salt (L-012) to detect superoxide in different biological systems. In human whole blood or isolated leukocytes, the sensitivity of the luminol analogue L-012 to detect superoxide was higher as compared with luminol, lucigenin, coelenterazine, ... More
Agonist-induced peroxynitrite production from endothelial cells.
AuthorsKooy NW, Royall JA
JournalArch Biochem Biophys
PubMed ID8179319
'Nitric oxide reacts with superoxide to form peroxynitrite, a potential mediator of oxidant-induced cellular injury. The endothelium is a primary target of injury in many pathological states, including acute lung injury, sepsis, multiple organ failure syndrome, and atherosclerosis, where enhanced production of nitric oxide and superoxide occurs simultaneously. It was ... More
Determination of lipid hydroperoxides in low density lipoprotein from human plasma using high performance liquid chromatography with chemiluminescence detection.
AuthorsMiyazawa T, Fujimoto K, Oikawa S
JournalBiomed Chromatogr
PubMed ID2383696
'A high performance liquid chromatographic system with chemiluminescence detection (HPLC-CL) was used for determining phospholipid hydroperoxides in human plasma low density lipoprotein (LDL). This system involved separation of phospholipids from LDL-total lipids with normal phase silica gel HPLC and post-column detection of hydroperoxide-dependent chemiluminescence produced by luminol oxidation during the ... More
Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier microscopy.
AuthorsSuematsu M, Oshio C, Miura S, Tsuchiya M
JournalBiochem Biophys Res Commun
PubMed ID3426608
'Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among ... More
Reactions of copper complexes with oxygen radicals generated by human neutrophils.
AuthorsHaseloff RF, Gruner S, Wischnewsky GG
JournalJ Biolumin Chemilumin
PubMed ID1325096
'The intensity of the chemiluminescence of unstimulated human neutrophils in the presence of luminol was used to investigate the effects of low-molecular-weight copper complexes at the cellular level. In different models (superoxide dismutase mimetic activity, inhibition of haematoporphyrin derivative/light-induced lysis of cells), the biological activity of the complexes exceeded the ... More
A chemiluminescent catecholamine assay: its application for monitoring adrenergic transmitter release.
AuthorsIsraël M, Tomasi M
JournalJ Neurosci Methods
PubMed ID10522828
'A chemiluminescent procedure for measuring catecholamines (dopamine, norepinephrine, epinephrine) is described. It is based on the observation that lactoperoxidase catalyses both the oxidation of catecholamines, and the chemiluminescent reaction of luminol with their oxidation product. The assay has been adapted for continuously monitoring the release of catecholamines from adrenergic tissues, ... More
Chemiluminescence intensities and spectra of luminol oxidation by sodium hypochlorite in the presence of hydrogen peroxide.
AuthorsArnhold J, Mueller S, Arnold K, Grimm E
JournalJ Biolumin Chemilumin
PubMed ID1746319
'Hydrogen peroxide amplifies the chemiluminescence in the oxidation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10(-8)-7.5 x 10(-6) mol/l. At 7.5 x 10(-6) mol/l H2O2 the chemiluminescence is amplified 550-fold. The chemiluminescence spectra ... More
Influence of polyclonal immunoglobulins on the polymorphonuclear leukocyte response to lipopolysaccharide of Salmonella enteritidis as measured with luminol-enhanced chemiluminescence.
AuthorsWagner DR, Heinrich D
JournalInfect Immun
PubMed ID7927690
'In gram-negative sepsis, the activation of polymorphonuclear leukocytes (PMN) by lipopolysaccharide (LPS) and the resulting production of superoxide and other oxygen radicals may be an important cause of tissue damage. A suppression of the PMN response to LPS stimulation would be therapeutically beneficial. The aim of this study was to ... More
Chemiluminescence detection for high-performance liquid chromatography of biomedical samples.
AuthorsDe Jong GJ, Kwakman PJ
JournalJ Chromatogr
PubMed ID2670996
'During recent years, much progress has been made in the development of high-performance liquid chromatographic (HPLC) detection systems based on chemiluminescence (CL). CL is now one of the most sensitive detection methods in HPLC. For many compounds detection limits in the femtogram to picogram range have been obtained. Several on-line ... More
Free radical production by the red tide alga, Chattonella antiqua.
AuthorsShimada M, Akagi N, Nakai Y, Goto H, Watanabe M, Watanabe H, Nakanishi M, Yoshimatsu S, Ono C
JournalHistochem J
PubMed ID1655681
'The red tide alga, Chattonella antiqua, was found to show a strong chemiluminescence, using luminol as the reagent, when exposed to ultraviolet irradiation. This luminescence was completely inhibited by ascorbate or catalase, suggesting that hydrogen peroxide was generated by the plankton. Red tide cells exposed to fish gill mucus from ... More
Hydroxyl radical generation: the effect of bicarbonate, dioxygen and buffer concentration on pH-dependent chemiluminescence.
AuthorsOosthuizen MM, Greyling D
JournalRedox Rep
PubMed ID11450981
'Enhanced chemiluminescence (CL) induced by HO* was detected using the primary enhancers luminol (Lum), isoluminol (ILum) and lucigenin (Luc), in the absence of HCO3-, at pH 9.5, 10.0 and 10.5 but not between pH 4.0 and 9.0. This was confirmed using nine different HO* generators. FeCl3/NTA/H2O2 was the only HO* ... More
Neutrophil oxidative metabolism and killing of P. brasiliensis after air pouch infection of susceptible and resistant mice.
AuthorsMeloni-Bruneri LH, Campa A, Abdalla DS, Calich VL, Lenzi HL, Burger E
JournalJ Leukoc Biol
PubMed ID8613700
'The oxidative burst of polymorphonuclear neutrophils (PMN) and their ability to inhibit Paracoccidioides brasiliensis growth was studied in susceptible (B10.A) and resistant (A/J) mice. The cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure PMN preparations; the number of cells was similar for both strains at 24 ... More
Enzymatic chemiluminescence assay for inorganic pyrophosphate.
AuthorsJansson V, Jansson K
JournalAnal Biochem
PubMed ID11969200
Mechanism of the luminol-dependent chemiluminescence of human neutrophils.
AuthorsDeChatelet LR, Long GD, Shirley PS, Bass DA, Thomas MJ, Henderson FW, Cohen MS
JournalJ Immunol
PubMed ID6286768
Evaluation of antioxidant capacity by chemiluminescence.
AuthorsVisioli F, Galli C
JournalAnal Biochem
PubMed ID9212878
Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages.
AuthorsAllen RC, Loose LD
JournalBiochem Biophys Res Commun
PubMed ID1259763
Development of chemiluminescence reactions in biomedical analysis.
AuthorsBaeyens WR, Nakashima K, Imai K, Ling BL, Tsukamoto Y
JournalJ Pharm Biomed Anal
PubMed ID2490747
Luminol and lucigenin as detectors for O2.-.
AuthorsFaulkner K, Fridovich I
JournalFree Radic Biol Med
PubMed ID8225026
Univalent oxidation of luminol and univalent reduction of lucigenin must precede reaction with O2.- if that reaction is to lead to luminescence. The assumption that luminol or lucigenin, per se, reacts with O2.- in a way leading to luminescence is incorrect, and leads to misinterpretation of results. The chemical reactions ... More
Analytical applications of liquid phase chemiluminescence reactions--a review.
AuthorsBowie AR, Sanders MG, Worsfold PJ
JournalJ Biolumin Chemilumin
PubMed ID8726582
This paper reviews the literature on analytical applications of quantitative liquid phase chemiluminescence (CL) from 1991 to mid-1995. Other relevant reviews in this general area are also cited to provide an historical perspective. The focus is on the two major analytical techniques used in conjunction with flow-through CL detection, namely ... More
Measurement of reactive oxygen species in cells and mitochondria.
AuthorsArmstrong JS, Whiteman M,
JournalMethods Cell Biol
PubMed ID17445704
This chapter discusses (1) the cellular sources of ROS and their enzymatic detoxification; (2) common methods used to determine cellular and mitochondrial ROS, including chemiluminescence (CL), spin trapping, and fluorescence and enzymatic techniques; (3) common problems associated with these assays and the interpretation of data; and (4) simple protocols for ... More
4-Phenylylboronic acid: a new type of enhancer for the horseradish peroxidase catalysed chemiluminescent oxidation of luminol.
AuthorsKricka LJ, Ji X
JournalJ Biolumin Chemilumin
PubMed ID7762416
4-Phenylylboronic acid enhances the light emission from the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide. Optimization studies showed that the greatest enhancement was obtained using micromolar concentrations of the new enhancer. The largest degree of enhancement was found with the basic isoenzyme of horseradish peroxidase (Type VIA), and ... More
A sensitive, rapid, chemiluminescence-based method for the determination of diamines and polyamines.
AuthorsBachrach U, Plesser YM
JournalAnal Biochem
PubMed ID3083714
A new sensitive and rapid chemiluminescence-based method for the determination of diamines and polyamines is described. Phosphocellulose paper strips are used for the removal of neutral or negatively charged molecules from polyamine-containing fluid. The procedure is based on the determination of hydrogen peroxide, produced during the oxidation of polyamines, by ... More
Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine.
AuthorsIsraël M, Lesbats B
JournalJ Neurochem
PubMed ID7045285
It is now possible to extend to mammalian tissues the chemiluminescent acetylcholine assay. Mammalian tissue extracts must be treated with oxidants (which is not necessary for electric organ extracts). The assay can then be performed as previously described (acetylcholinesterase hydrolyses acetylcholine; choline oxidase converts choline to betaine and H2O2, which ... More
A new technique for enhancing luminol luminescent detection of free radicals and reactive oxygen species.
AuthorsTrevithick JR, Dzialoszynski T
JournalBiochem Mol Biol Int
PubMed ID7804144
A novel technique is reported which makes use of (1) an improved method for solubilizing luminol at neutral pH along with (2) the addition of dimethyl sulfoxide to stabilize superoxide anion in the solutions in which luminescence is detected. These improvements resulted in (1) very low blank values of luminescence ... More
Chemiluminescent assays for choline and phospholipase-D using a flow injection system.
AuthorsYaqoob M, Nabi A, Masoom-Yasinzai M
JournalJ Biolumin Chemilumin
PubMed ID9427112
A flow injection chemiluminescent method is described for the determination of choline. The method is based on the production of hydrogen peroxide from choline using on-line covalently bound immobilized choline oxidase column. The product is mixed downstream and detected via the cobalt catalyzed chemiluminescent oxidation of luminol. The detection limit ... More
A simple chemiluminescence assay for the determination of reactive oxygen species produced by human neutrophils.
AuthorsLiu L, Dahlgren C, Elwing H, Lundqvist H
JournalJ Immunol Methods
PubMed ID8699014
We show that phagocyte production of reactive oxygen species can be measured using a microtitre plate based chemiluminescence blotting technique. The production of reactive oxygen species is determined by their ability to catalyze the oxidation of luminol or isoluminol, resulting in light emission which is recorded on a photographic film. ... More
Unraveling peroxynitrite formation in biological systems.
AuthorsRadi R, Peluffo G, Alvarez MN, Naviliat M, Cayota A
JournalFree Radic Biol Med
PubMed ID11182518
Peroxynitrite promotes oxidative damage and is implicated in the pathophysiology of various diseases that involve accelerated rates of nitric oxide and superoxide formation. The unambiguous detection of peroxynitrite in biological systems is, however, difficult due to the combination of a short biological half-life, limited diffusion, multiple target molecule reactions, and ... More
Rapid detection and counting of single bacteria in a wide field using a photon-counting TV camera.
AuthorsMasuko M, Hosoi S, Hayakawa T
JournalFEMS Microbiol Lett
PubMed ID1778434
Using Escherichia coli as a model bacterium, we tested a photon-counting method for enumeration of bacteria. This method is based on the principle that microscopic sized luminous particles in a wide field can be directly detected and counted using a photon-counting TV camera without the use of a microscope. E. ... More
A chemiluminescence reaction between hydrogen peroxide and acetonitrile and its applications.
AuthorsLu J, Lau C, Morizono M, Ohta K, Kai M
JournalAnal Chem
PubMed ID11791569
Here we report a new chemiluminescence reaction between basic aqueous H2O2 and acetonitrile. Its ultraweak chemiluminescence could be greatly enhanced by luminol, isoluminol-labeled streptavidin, and an Edman-type fluorescent reagent. Light emission was intense and long-lived, and this facilitated the initiation of the reaction and the measurement of the light emission. ... More
Presence of phosphatidylcholine hydroperoxide in human plasma.
AuthorsMiyazawa T, Yasuda K, Fujimoto K, Kaneda T
JournalJ Biochem (Tokyo)
PubMed ID2846518
A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through ... More
Potential role of the peroxidase-dependent metabolism of serotonin in lowering the polymorphonuclear leukocyte bactericidal function.
AuthorsSalman-Tabcheh S, Guérin MC, Torreilles J
JournalFree Radic Res
PubMed ID8747893
Serotonin (5-hydroxytryptamine, 5-HT) significantly and dose-dependently suppressed the luminol-enhanced chemiluminescence (CL) signal generated by polymorphonuclear leukocytes (PMN) activated with phorbol myristate acetate (PMA), but did not modify either lucigenin-enhanced CL or the reduction of superoxide dismutase-inhibitable cytochrome c. Moreover, stimulation of PMNs previously incubated with 5-HT resulted in a threefold ... More
Photochemiluminescent detection of antiradical activity; III: A simple assay of ascorbate in blood plasma.
AuthorsLewin G, Popov I
JournalJ Biochem Biophys Methods
PubMed ID7963248
A simple procedure to determine the ascorbate in blood plasma was elaborated based on a temporary inhibition of the photo-induced, O(-.)2-mediated, chemiluminescence emanating from oxidation of luminol. To remove the interferences, mainly from urate, a simple enzyme-free one-step method for plasma sample preparation by means of a gel filtration was ... More
Enhanced chemiluminescent sandwich enzyme immunoassay for hen egg lysozyme.
AuthorsRauch P, Poplstein M, Hochel I, Fukal L, Ferri E, Abagnato CA, Girotti S, Roda A
JournalJ Biolumin Chemilumin
PubMed ID7762414
A sensitive chemiluminescent sandwich-type enzyme immunoassay for hen egg lysozyme was developed. The assay was performed on polystyrene microtitre plates using immobilized specific polyclonal rabbit antibody against lysozyme, a peroxidase conjugate and the H2O2/luminol-enhanced chemiluminescence detection reagent. The chemiluminescent signal was detected using either a microplate luminometer, or photographic film ... More
Luminol chemiluminescence using xanthine and hypoxanthine as xanthine oxidase substrates.
AuthorsRadi R, Rubbo H, Thomson L, Prodanov E
JournalFree Radic Biol Med
PubMed ID2158934
Luminol chemiluminescence induced by the xanthine or hypoxanthine-O2-xanthine oxidase system is analyzed and compared. Characteristics of the light emission curves were examined considering the conventional reaction scheme for the oxidation of both substrates in the presence of xanthine oxidase. The ratio of the areas of the rate of superoxide production ... More
Co-oxidation of luminol by hypochlorite and hydrogen peroxide implications for neutrophil chemiluminescence.
AuthorsBrestel EP
JournalBiochem Biophys Res Commun
PubMed ID2982377
Stimulated neutrophils produce several potent oxidants including H2O2, O2- and HOCl. Previous studies have revealed all of these compounds to be capable of oxidizing luminol, a reagent often used to indicate, by its chemiluminescence, the oxidative burst of neutrophils. Data presented in this paper indicate that H2O2 and HOCl spontaneously ... More
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
AuthorsWardman P,
JournalFree Radic Biol Med
PubMed ID17761297
Chemical probes for free radicals in biology are important tools; fluorescence and chemiluminescence offer high detection sensitivity. This article reviews progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use. Reactive species include hydrogen peroxide; hydroxyl, superoxide, and thiyl radicals; carbonate ... More
Superoxide production by normal and chronic granulomatous disease (CGD) patient-derived EBV-transformed B cell lines measured by chemiluminescence-based assays.
AuthorsPorter CD, Parkar MH, Collins MK, Levinsky RJ, Kinnon C
JournalJ Immunol Methods
PubMed ID1331241
We have compared assays for products of the neutrophil respiratory burst in normal EBV-transformed B cell lines stimulated with agonists of protein kinase C. Those measuring O2- directly or its immediate product, H2O2, were successful. Of these, the most sensitive were the lucigenin- and luminol-based chemiluminescence assays for O2- and ... More
Granulocyte colony-stimulating factor enhances the extracellular emission of reactive oxygen from neutrophils stimulated with formylmethionylleucylphenylalanine.
AuthorsKatsura Y, Tsuru S, Noritake M, Kayashima S, Wakiyama H, Shinomiya N, Shinomiya M, Rokutanda M
JournalCell Immunol
PubMed ID7684324
Pretreatment of neutrophils with granulocyte colony-stimulating factor (G-CSF) enhanced luminol-dependent chemiluminescence emission by the stimulation of formylmethionylleucylphenylalanine (FMLP), opsonized zymosan, and zymosan. The intensity of the priming effects was in the following order: FMLP > zymosan > opsonized zymosan. However, the priming effect of G-CSF was not detected by the ... More
What do we measure by a luminol-dependent chemiluminescence of phagocytes?
AuthorsVilim V, Wilhelm J
JournalFree Radic Biol Med
PubMed ID2546866
The review presents a survey of published findings concerning the mechanism of luminol-dependent chemiluminescence in biological systems. The potential of various oxygen species (superoxide anion, hydrogen peroxide, hydroxyl radical) to react with luminol is discussed. The ability of commonly used enzymes (superoxide dismutase, catalase), inhibitors, and oxygen radical scavengers to ... More
Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite.
AuthorsArnhold J, Mueller S, Arnold K, Sonntag K
JournalJ Biolumin Chemilumin
PubMed ID8285109
Two different mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite were found. Most substances investigated in these experiments acted by scavenging NaOCl. This mechanism was independent of the concentration of hydrogen peroxide and the incubation time between luminol and inhibitors. The most potent inhibitors were ... More
Interaction of chlormezanone enantiomers with rat liver microsomes.
AuthorsKlinger W, Oelschläger H, Rothley D, Karge E, Seeling A
JournalEur J Drug Metab Pharmacokinet
PubMed ID10412893
Both chlormezanone enantiomers, for the first time obtained by enantiospecific HPLC with a 100% yield, bind to oxidized cytochrome P-450 in rat liver microsomes with a binding curve according to type I, similar to hexobarbital but less pronounced. There are no differences between the binding curves of the two enantiomers. ... More
Oxidative damage of human skin lipids. Dependence of lipid peroxidation on sterol concentration.
AuthorsLasch J, Schönfelder U, Walke M, Zellmer S, Beckert D
JournalBiochim Biophys Acta
PubMed ID9421189
Photoprotection against sunburn and associated irradiation-induced damages of the human skin is mainly attributed to the darkening of the biochrome melanin by its oxidation. Human skin lipids were examined for an additional protection by sterols. Lipid vesicles prepared from extracted human skin lipids as well as from mixtures of typical ... More
Practical methods for detection of nitric oxide.
AuthorsNagano T
JournalLuminescence
PubMed ID10602296
Nitric oxide (NO), which is generated in vivo through conversion of L-arginine to L-citrulline by NO synthase (NOS), mediates many physiological and pathophysiological processes. At least two distinct isoforms of NOS have been identified, constitutive NOS (cNOS) and inducible NOS (iNOS). The cNOS, which is constitutively expressed in endothelial cells ... More
Charge-coupled device camera-based detection of fluorescence-labeled proteins immobilized on nitrocellulose membranes.
AuthorsGraf R, Friedl P
JournalElectrophoresis
PubMed ID11332757
Proteins dotted on nitrocellulose membranes are biotinylated by reaction with a biotinyl-succinimide ester. The resulting biotinyl residues serve as specific binding sites for a subsequent streptavidin-based detection system. Using streptavidin-peroxidase, the proteins are visualized either by deposition of a colored formazan dye or by enhanced chemiluminescence the latter being twofold ... More
Interaction between neutrophil-derived elastase and reactive oxygen species in cartilage degradation.
AuthorsIwamura H, Moore AR, Willoughby DA
JournalBiochim Biophys Acta
PubMed ID8461320
The role of proteases and reactive oxygen species (ROS) in polymorphonuclear neutrophil (PMN) induced cartilage degradation in vitro were studied. ONO-5046, a novel synthetic elastase inhibitor, significantly and dose dependently protected cartilage from degradation induced by PMNs stimulated with phorbol myristate acetate (PMA), opsonized zymosan, N-formyl-methionyl-leucyl-phenylalanine plus cytochalasin-B, or A-23187. ... More
Chemiluminescent response to PMA in isolated rat liver after in situ ischemia-reperfusion.
AuthorsIwata S, Jamieson DD, Chance B
JournalFree Radic Biol Med
PubMed ID8138182
The median and left lateral lobes of rat liver in situ were rendered ischemic for 30 min, then blood flow reinstituted. After 1, 3, 6, 24, or 48 h, livers were removed and set up for isolated perfused organ study. Luminol enhanced chemiluminescence (LEC) was recorded from the surface of ... More