Novex™ Tricine SDS 上样缓冲液 (2X)
Novex™ Tricine SDS 上样缓冲液 (2X)
Invitrogen™

Novex™ Tricine SDS 上样缓冲液 (2X)

Novex Tricine SDS 样品缓冲液 (2X) 可为使用 Tricine 凝胶进行的变性凝胶电泳制备蛋白样品。Novex Tricine了解更多信息
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货号数量
LC167620 mL
货号 LC1676
价格（CNY)
380.00
Each
添加至购物车
数量:
20 mL
价格（CNY)
380.00
Each
添加至购物车
Novex Tricine SDS 样品缓冲液 (2X) 可为使用 Tricine 凝胶进行的变性凝胶电泳制备蛋白样品。Novex Tricine SDS 样品缓冲液专为优化小分子量蛋白质和多肽的电泳分离而配制。配制此样品缓冲液时以考马斯蓝 G 和酚红作为示踪染料——而非溴酚蓝。采用 Tricine SDS 电泳缓冲液时，考马斯蓝 G 中具有清晰的染料前沿，与溴酚蓝的移动离子前沿迁移距离更接近。溴酚蓝的电泳速度比部分多肽更慢。这可确保小尺寸肽段不会在凝胶上迁移。

使用方法：在 85°C 下，加热 1X 稀释液（还原或非还原状态）中的样品 2 分钟，以获得理想的结果。

推荐的缓冲液：Tricine 电泳缓冲液须搭配 Tricine 样品缓冲液使用，方可充分发挥该凝胶系统的优点。

查看所有 SDS-PAGE 可用的缓冲液和试剂›
仅供科研使用。不可用于诊断程序。
规格
化学名称或材料上样缓冲液
建议的储存条件Tricine 上样缓冲液 (2X) 含十二烷基硫酸钠 (SDS) 及考马斯蓝 G 和酚红，pH 8.45。

2°C 至8°C 储存。

最大浓度2 X
物理形态Liquid
产品线Novex™
数量20 mL
Unit SizeEach

常见问题解答 (FAQ)

尿素能否用于Tricine凝胶系统，以获得变性结果？

在SDS发挥的变性作用不充分时，向样品和电泳缓冲液中加尿素，与SDS共同作用，可能会改善样品的溶解性。这必须经过对目标蛋白质的经验验证。

How long should I run the Novex Tricine Gels (e.g. Cat. No. EC6675BOX) and how do I recognize the running front?

You should run the gel until the phenol red tracking dye from the Novex Tricine SDS Sample Buffer (Cat. No. LC1676) reaches the bottom of the gel. Phenol red serves as an indicator of the running front as it is a very small molecule that migrates with the ion front in Tricine gels. The Coomassie from the sample buffer runs a little slower and can be 1-2 cm behind the phenol red.

Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.

Does the Novex Tricine SDS Sample Buffer (2X) (Cat. No. LC1676) contain Dithiothreitol (DTT)?

Novex Tricine SDS Sample Buffer (2X), does not contain DTT or other reducing agents.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can urea be used with the Tricine gel system to achieve denatured results?

Adding urea to the sample and running buffers, in conjunction with SDS, may provide improved solubilization of the sample if denaturation by SDS does not prove to be sufficient. This must be tested empirically for the protein of interest.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.