Incubation Tray, 10 x 14 cm - FAQs

查看更多产品信息 Incubation Tray, 10 x 14 cm - FAQs (LC2102)

6 个常见问题解答

If a green color appears on the membrane after the transfer using the iBlot 2 Dry Blotting System, will it affect the results?

The green discoloration is copper deposits from the transfer stack, and it does not affect the results. To minimize this effect, shake excess water off the filter paper and buffer from the gel before placing each on the stack. The current formulation of stacks minimizes the green discoloration.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the bottom stack of my iBlot 2 Transfer Stack to fit my separating gel?

No. Do not trim the iBlot 2 Transfer Stacks to fit your gel size.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the PVDF membrane in the iBlot 2 PVDF Gel Transfer Stack pre-activated?

The PVDF membrane is pre-activated and ready for use without any pre-treatment with alcohol.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the stack and/or absorbent pad more than once when using the iBlot 2 Dry Blotting System?

No. The transfer stacks have a finite amount of ions to drive the transfer and are depleted after a single use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I perform multiple transfers, with a new transfer starting immediately after the previous one when using the iBlot 2 Dry Blotting System?

Yes. The device is designed to do so with no impact on performance.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I create my own transfer conditions with the iBlot 2 Dry Blotting System?

Yes. The device allows programming custom methods.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.