Identification of components of protein complexes using a fluorescent photo-cross-linker and mass spectrometry.
Authors Wine Robert N; Dial John M; Tomer Kenneth B; Borchers Christoph H;
JournalAnal Chem
PubMed ID12033289
'This study describes a novel method for improving the specific recognition, detection, and identification of proteins involved in multiprotein complexes. The method is based on a combination of coimmunoprecipitation, chemical cross-linking, and specific fluorescent tagging of protein components in close association with one another. Specific fluorescent tagging of the protein ... More