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IEF Cathode Buffer pH 3-10 (10X) - FAQs

查看更多产品信息 IEF Cathode Buffer pH 3-10 (10X) - FAQs (LC5310)

5 个常见问题解答

你们是否提供用于蛋白质凝胶染色的染色盘(staining trays)？

我们提供以下蛋白质凝胶染色盘：

•StainEase 染色盘（货号NI2400）可用于对小型和中型凝胶进行方便、均匀的染色。该染色盘组件包括一个用于放置凝胶和排出染料的多孔滤管嵌件、一个盖子以及不必操作凝胶即可在其中更换染料和溶液的外部容器。可轻松安装最多4块小型凝胶。
•小型凝胶孵育盘（货号22843）可用于小型凝胶和蛋白免疫印迹膜的染色。

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the components of the ZOOM IEF Fractionator?

The ZOOM IEF Fractionator includes the following components:

Chamber Assembly Tube with Anode Reservoir
Spill Trough with Cathode Reservoir Lid
Sample Chambers (7)
Sample Chamber O-ring Seals, red (10)
Sample Chamber Caps with O-rings (7)
Cathode End Sealer
Anode End Sealer
Cathode End Screw Cap
Spacers, black (8)
Spares Box 1
Sample Chamber O-ring Seals (8)
Sample Chamber Caps with O-rings (7)
Spares Box 2
Cathode Chamber Seals (2)
Spacers, black (8)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the storage conditions for the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer?

We recommend storing the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.