Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311 - FAQs

查看更多产品信息 Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311 - FAQs (LC5317)

2 个常见问题解答

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.