Novex™ IEF 阴极缓冲液 pH 3-7 (10X)
Novex™ IEF 阴极缓冲液 pH 3-7 (10X)
Invitrogen™

Novex™ IEF 阴极缓冲液 pH 3-7 (10X)

Novex™ IEF 阴极缓冲液 pH 3-7 (10X) 是经过优化的预混合 IEF 阴极缓冲液,适合与 Novex™了解更多信息
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货号数量
LC5370125 mL
货号 LC5370
价格(CNY)
1,635.00
Each
添加至购物车
数量:
125 mL
价格(CNY)
1,635.00
Each
添加至购物车
Novex™ IEF 阴极缓冲液 pH 3-7 (10X) 是经过优化的预混合 IEF 阴极缓冲液,适合与 Novex™ pH 3-7 IEF 凝胶配合使用。IEF 是受许多因素影响的灵敏技术。优化后的预混 IEF 缓冲液减少了不定因素,可确保一致的结果。

使用说明:IEF 阴极缓冲液中含有颜色可在白色至淡黄色之间变换的试剂。这种颜色变换可能导致终溶液颜色出现类似变化,在白色至淡黄色之间变换。这种颜色变换不会影响产品自身性能。
仅供科研使用。不可用于诊断程序。
规格
缓冲液阳极&阴极缓冲液
最大浓度10 X
凝胶兼容性Novex™ IEF 凝胶
产品线Novex™
产品类型IEF 阴极缓冲液
数量125 mL
运输条件湿冰
凝胶类型等电聚焦(IEF)
pH 范围3 - 7
Unit SizeEach
内容与储存
储存在冰箱 (2–8°C) 中。

常见问题解答 (FAQ)

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.