iBright™ Prestained Protein Ladder
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iBright™ Prestained Protein Ladder
Invitrogen™

iBright™ Prestained Protein Ladder

iBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct了解更多信息
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货号数量
LC56152 x 250μL
货号 LC5615
价格(CNY)
1,975.00
Each
添加至购物车
数量:
2 x 250μL
价格(CNY)
1,975.00
Each
添加至购物车
iBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

Product features
• Fluorescent visualization—detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager
• Western blot confirmation—detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein

Applications
• Western blotting: detection of the two unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies.
• Protein sizing on SDS-polyacrylamide gels, visualization of proteins during electrophoresis and transfer, and visualization of proteins during fluorescence imaging (ten blue-stained bands)
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using near-infrared (NIR) fluorescence imagers

仅供科研使用。不可用于诊断程序。
规格
检测方法比色、NIR 荧光和用户定义检测系统(2个条带)
凝胶兼容性Novex™ 中型凝胶、Novex™ 小型凝胶、Novex™ Tris-甘氨酸凝胶、NuPAGE™ Bis-Tris 凝胶、NuPAGE™ 凝胶、NuPAGE™ MES 凝胶、NuPAGE™ MOPS 凝胶、NuPAGE™ Tris 醋酸盐凝胶、Precise™ Tris-甘氨酸凝胶、SDS-PAGE 凝胶、Precise™ Tris-HEPES 凝胶
分子量250、130、95、80、70、55、43、34、30、26、15、11 kDa
产品线iBright™
产品类型蛋白分子量标准品
数量2 x 250μL
即用型
运输条件干冰
染色剂类型单色:蓝色
系统类型Western 印迹,SDS-PAGE
标记物数量12
大小范围11-250 kDa
Unit SizeEach
内容与储存
储存缓冲液:62.5 mM Tris-H3PO4(25°C 下 pH 值为 7.5)、1 mM EDTA、2% (w/v) SDS、10 mM DTT、1 mM NaN3 和 33% 甘油

接收后,储存于 -20°C 下。产品置于干冰上运输。

常见问题解答 (FAQ)

What gel running buffer should I use with the iBright Prestained Protein Ladder? Tris-glycine, Tris-tricine, or Tris-acetate running buffer?

Most of the common gel running buffers are composed of Tris-glycine or Tris-tricine. Tris-glycine buffer systems are useful for separation of proteins over a wide range of molecular weights (5-300 kDa) and are compatible with denaturing or non-denaturing conditions. Tris-tricine buffer is generally recommended for the electrophoresis of low molecular weight proteins and peptides (<10 kDa) that need to be reduced and denatured prior to loading. Tris-acetate buffer system is used for separation of larger proteins (>200 kDa).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the iBright Prestained Protein Ladder be detected by IgM antibodies?

The 2 unstained bands in the iBright Prestained Ladder contain an IgG binding site, allowing direct visualization with the same antibody-conjugate reagents used to detect the target protein. The proteins in the standard will not bind to IgM antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the iBright Prestained Protein Ladder, I am unable to detect the 2 unstained protein bands. Why is this?

Primary antibodies with low starting concentrations may result in insufficient chemiluminescent detection of the western blot positive control bands. If the unstained 30 kDa and 80 kDa bands produce weak or no signal, spike the diluted primary antibody with the corresponding rabbit IgG or mouse IgG to a concentration of 1-5 µg/mL, prior to secondary antibody incubation. Follow with respective secondary (GAM/GAR) incubation to increase the intensity of western blot positive control bands in the iBright Prestained Protein Ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the recommended gel loading volume for the iBright Prestained Protein Ladder?

If performing detection on a mini gel, the recommended loading volume is 1-3 µL. If performing visualization on a midi gel, 2-4 µL is recommended, and for detection, 2-3 µL is recommended. Optimal loading volume should be determined empirically.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I use the iBright Prestained Protein Ladder over other protein ladders?

The iBright Prestained Protein Ladder has been optimized and designed for use with western blotting as it provides 2 control bands (unstained ladder bands) that are detected using the same antibody conjugate and protocol. The 2 unstained control bands contain IgG binding sites that can be visualized simultaneously with your target without any additional steps in the protocol. The 10 stained bands will appear during electrophoresis and transfer. These 10 bands will also appear in fluorescent imaging in the NIR channels. The 2 control bands will appear with chemiluminescent or fluorescent detection similar to your target.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.