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引用和文献 (3)
Invitrogen™
InVision™ His-Tag 胶内染色试剂盒
The InVision His-tag In-gel Staining Kit includes InVision His-tag In-gel Stain as well as BenchMark His-tagged Protein Standard. InVision His-tag了解更多信息
| 货号 | 数量 |
|---|---|
| LC6033 | 1 kit |
货号 LC6033
价格（CNY)
11,362.00
Each
数量:
1 kit
价格（CNY)
11,362.00
Each
The InVision His-tag In-gel Staining Kit includes InVision His-tag In-gel Stain as well as BenchMark His-tagged Protein Standard. InVision His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis.
Features:
• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours
Compare all His-tag stains ›
Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.
Features:
• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours
Compare all His-tag stains ›
Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.
仅供科研使用。不可用于诊断程序。
规格
检测定位凝胶内检测
检测方法荧光
产品线InVision
产品类型His 标签蛋白胶内染色试剂盒
数量1 kit
靶标分子蛋白质（His 标记）
标签或染料InVision His-Tag 染料
Unit SizeEach
内容与储存
InVision™ His 标签胶内染料以 1X 即用型染色试剂 (500 mL) 的形式提供。试剂盒中还包含 BenchMark™ His 标签蛋白标准品 (125 μL)。
常见问题解答 (FAQ)
我使用InVision His-Tag In-Gel Stain对His标签融合蛋白染色，并使用BenchMark His标签蛋白标准品作为阳性对照。结果看到BenchMark His标签蛋白标准品出现一些多余条带。问题出在哪里？
我在使用InVisionHis-Tag In-Gel Stain时，得到了较高的、不均匀的背景。你们有何建议？
我在使用InVisionHis-Tag In-Gel Stain时，得到的染色效果很弱。你们有何建议？
InVision His-Tag In-Gel Stain能否用于E-PAGE凝胶染色？
经过InVision His-Tag In-Gel Stain染色的His标签融合蛋白，能否用于免疫印迹分析？
引用和文献 (3)
引用和文献
Abstract
Novel determinant of PKC-epsilon anchoring at cardiac Z-lines.
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:15964917
'The Z-line represents a critical link between the transverse tubule network and cytoskeleton of cardiac cells with a role in anchoring structural proteins, ion channels, and signaling molecules. Protein kinase C-epsilon (PKC-epsilon) regulates cardiac excitability, cardioprotection, and growth, possibly as a consequence of translocation to the Z-line/T tubule region. To
Dss1 interaction with Brh2 as a regulatory mechanism for recombinational repair.
Journal:Mol Cell Biol
PubMed ID:17261595
Brh2, the BRCA2 ortholog in Ustilago maydis, enables recombinational repair of DNA by controlling Rad51 and is in turn regulated by Dss1. Interplay with Rad51 is conducted via the BRC element located in the N-terminal region of the protein and through an unrelated domain, CRE, at the C terminus. Mutation
A conserved inverted repeat, the LipR box, mediates transcriptional activation of the Streptomyces exfoliatus lipase gene by LipR, a member of the STAND class of P-loop nucleoside triphosphatases.
Journal:J Bacteriol
PubMed ID:17015647
Expression of the Streptomyces exfoliatus lipA gene, which encodes an extracellular lipase, depends on LipR, a transcriptional activator that belongs to the STAND class of P-loop nucleoside triphosphatases. LipR is closely related to activators present in some antibiotic biosynthesis clusters of actinomycetes, forming the LipR/TchG family of regulators. In this