Novex™ Hi-Density TBE Sample Buffer (5X) - FAQs

查看更多产品信息 Novex™ Hi-Density TBE Sample Buffer (5X) - FAQs (LC6678)

4 个常见问题解答

我的TBE缓冲液出现沉淀。我该怎么办？

如果出现了轻微的浑浊，可以在110°C高压灭菌5分钟溶解沉淀。不要在原装的容器内灭菌。这样做不会对TBE的缓冲性质产生不良影响。

你们有TBE电泳缓冲液和Hi-Density TBE上样缓冲液的配方吗？

请参见以下配方。

TBE电泳缓冲液（5X）：
Tris碱, 54.0 g
硼酸，27.5 g
EDTA（游离酸），2.9 g
加去离子水至1.0 L

Hi-Density TBE上样缓冲液(5X):
5X TBE电泳缓冲液（货号LC6675)），2 mL
Ficoll Type 400, 1.5 g
溴酚蓝，1 mL1%溶液
二甲苯青1 mL1%溶液
加去离子水至10.0 mL

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Do you have the recipe for the TBE running buffer and the Hi-Density TBE Sample Buffer?

Please see the recipes below:

TBE Running Buffer (5X):
Tris base, 54.0 g
Boric acid, 27.5 g
EDTA (free acid), 2.9 g
Deionized water to 1.0 L

Hi-Density TBE Sample Buffer (5X):
5X TBE Running buffer (Cat. No. LC6675), 2 mL
Ficoll Type 400, 1.5 g
Bromophenol blue, 1 mL of 1% solution
Xylene cyanol 1 mL of 1% solution
Deionized water to 10.0 mL