Comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases.
AuthorsYoshimura T, Sonoda KH, Sugahara M, Mochizuki Y, Enaida H, Oshima Y, Ueno A, Hata Y, Yoshida H, Ishibashi T
JournalPLoS One
PubMed ID19997642
'Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal vein occlusion (BRVO, n ... More
Comparison of commercial kits to measure cytokine responses to Plasmodium falciparum by multiplex microsphere suspension array technology.
AuthorsBerthoud TK, Manaca MN, Quelhas D, Aguilar R, Guinovart C, Puyol L, Barbosa A, Alonso PL, Dobaño C
JournalMalar J
PubMed ID21554671
'Multiplex cytokine profiling systems are useful tools for investigating correlates of protective immunity. Several Luminex and flow cytometry methods are commercially available but there is limited information on the relative performance of different kits. A series of comparison experiments were carried out to determine the most appropriate method for our ... More
Effects of docosahexaenoic acid-rich n-3 fatty acid supplementation on cytokine release from blood mononuclear leukocytes: the OmegAD study.
AuthorsVedin I, Cederholm T, Freund Levi Y, Basun H, Garlind A, Faxén Irving G, Jönhagen ME, Vessby B, Wahlund LO, Palmblad J,
JournalAm J Clin Nutr
PubMed ID18541548
'Dietary fish or fish oil rich in n-3 fatty acids (n-3 FAs), eg, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), ameliorate inflammatory reactions by various mechanisms. Whereas most studies have explored the effects of predominantly EPA-based n-3 FAs preparations, few have addressed the effects of n-3 FAs preparations with DHA ... More
Isolation of immunoresistant human glioma cell clones after selection with alloreactive cytotoxic T lymphocytes: cytogenetic and molecular cytogenetic characterization.
AuthorsGomez GG, Varella-Garcia M, Kruse CA,
JournalCancer Genet Cytogenet
PubMed ID16527606
'Intratumoral heterogeneity and genetic instability within gliomas may allow intrinsically immunoresistant (IR) cells to escape alloreactive cytotoxic T lymphocyte (aCTL) cellular immunotherapy. The potential existence of aCTL-resistant variants prompted us to investigate whether cellular immunotherapy resistant glioma models could be isolated. To generate the models, repeated intermittent or continuous selective ... More
Cytokine mapping of sera from women with preeclampsia and normal pregnancies.
AuthorsJonsson Y, Rubèr M, Matthiesen L, Berg G, Nieminen K, Sharma S, Ernerudh J, Ekerfelt C
JournalJ Reprod Immunol
PubMed ID16388854
Preeclampsia is a pregnancy-specific syndrome. The immune system in preeclampsia is changed with an increased innate activity and there is a hypothesis of a shift towards Th1-type immunity. The aim of this study was to determine a spectrum of soluble immunological factors denoting different aspects of immune activation in third ... More
Elevation of cerebrospinal fluid interleukin-1ß in bipolar disorder.
AuthorsSöderlund J, Olsson SK, Samuelsson M, Walther-Jallow L, Johansson C, Erhardt S, Landén M, Engberg G
JournalJ Psychiatry Neurosci
PubMed ID21138659
In recent years, a role for the immune system in the pathogenesis of psychiatric diseases has gained increased attention. Although bipolar disorder appears to be associated with altered serum cytokine levels, a putative immunological contribution to its pathophysiology remains to be established. Hitherto, no direct analyses of cerebrospinal fluid (CSF) ... More
Salivary cytokine levels in subjects with chronic periodontitis and in periodontally healthy individuals: a cross-sectional study.
AuthorsTeles RP, Likhari V, Socransky SS, Haffajee AD
JournalJ Periodontal Res
PubMed ID19210336
Saliva has been proposed as a noninvasive diagnostic fluid that could be used in the diagnosis of oral and systemic diseases. The levels of salivary biomarkers, such as cytokines, could potentially be used as a surrogate to distinguish periodontally healthy individuals from subjects with periodontitis. Therefore, the goal of the ... More
Allergic sensitisation in tuberculosis patients at the time of diagnosis and following chemotherapy.
AuthorsEllertsen LK, Storla DG, Diep LM, Brokstad KA, Wiker HG, Hetland G
JournalBMC Infect Dis
PubMed ID19558655
It is still a matter of debate whether there is an association between infection with Mycobacterium tuberculosis (M. tuberculosis) and allergy. Previously, we have shown higher levels of specific IgE to different inhalant allergens and total IgE in tuberculosis (TB) patients compared to controls. The objectives of this study were ... More
Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.
AuthorsLambert C, Li J, Jonscher K, Yang TC, Reigan P, Quintana M, Harvey J, Freed BM,
JournalJ Biol Chem
PubMed ID17491020
Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha,beta-unsaturated aldehydes in cigarette smoke (acrolein and crotonaldehyde) inhibited production of interleukin-2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T ... More
Adenosine production by human B cells and B cell-mediated suppression of activated T cells.
AuthorsSaze Z, Schuler PJ, Hong CS, Cheng D, Jackson EK, Whiteside TL,
Journal
PubMed ID23678003
Antibody-independent role of B cells in modulating T-cell responses is incompletely understood. Freshly isolated or cultured B cells isolated from the peripheral blood of 30 normal donors were evaluated for CD39 and CD73 coexpression, the ability to produce adenosine 5'-monophosphate (AMP) and adenosine (ADO) in the presence of exogenous adenosine ... More
Cutaneous microdialysis: cytokine evidence for altered innate reactivity in the skin of psoriasis patients?
AuthorsSjögren F, Davidsson K, Sjöström M, Anderson CD,
JournalAAPS J
PubMed ID22374383
Cutaneous microdialysis demonstrates cytokine production in living human skin. In the present study, microdialysis samples taken from uninvolved and lesional skin in three test subjects with psoriasis over 24 h have been investigated for cytokine content with a bead-based multiplex immunoassay from Luminex. Concentration curves for a set of Th1/Th2 and ... More
Are cutaneous microdialysis cytokine findings supported by end point biopsy immunohistochemistry findings?
AuthorsSjögren F, Anderson CD,
JournalAAPS J
PubMed ID20967522
Insertion of a cutaneous microdialysis catheter into normal dermis has been shown to induce the production of IL1b, IL6 and IL8 in an innate response to minimal trauma. In the present study, skin biopsy for immunohistochemistry has been performed at the site of the microdialysis catheter to compare the findings ... More
Analysis of inflammatory cytokines in the tears of dry eye patients.
AuthorsMassingale ML, Li X, Vallabhajosyula M, Chen D, Wei Y, Asbell PA,
JournalCornea
PubMed ID19724208
To determine the levels of 8 important cytokines and 1 chemokine in tears of patients with dry eye disease. Tear samples were collected from 7 patients with dry eye disease and 7 healthy volunteers, and impression cytology samples were collected from 3 of the dry eye patients and 3 of ... More
Determination of cytokine protein levels in cervical mucus samples from young women by a multiplex immunoassay method and assessment of correlates.
AuthorsLieberman JA, Moscicki AB, Sumerel JL, Ma Y, Scott ME,
JournalClin Vaccine Immunol
PubMed ID17978011
Cytokines in cervical mucus are likely to play important roles in controlling pathogens. The cervical mucosal environment is complex, however, with many endogenous and exogenous factors that may affect cytokine levels. We used a multiplex, suspension-array-based immunoassay method to measure 10 proinflammatory (interleukin-1beta [IL-1beta], IL-6, and IL-8) and immunoregulatory (gamma ... More
An experimental model of rhinovirus induced chronic obstructive pulmonary disease exacerbations: a pilot study.
AuthorsMallia P, Message SD, Kebadze T, Parker HL, Kon OM, Johnston SL,
JournalRespir Res
PubMed ID16956406
Acute exacerbations of COPD are a major cause of morbidity, mortality and hospitalisation. Respiratory viruses are associated with the majority of exacerbations but a causal relationship has not been demonstrated and the mechanisms of virus-induced exacerbations are poorly understood. Development of a human experimental model would provide evidence of causation ... More
IL-23 up-regulates IL-10 and induces IL-17 synthesis by polyclonally activated naive T cells in human.
AuthorsVanden Eijnden S, Goriely S, De Wit D, Willems F, Goldman M,
JournalEur J Immunol
PubMed ID15682457
Interleukin (IL)-23 is a heterodimeric cytokine of the IL-12 family. Human IL-23 is known to induce interferon (IFN)-gamma production and proliferation in T cells, preferentially in the CD45RO+ memory subset. Yet, its role in the differentiation of human naive T cells remains largely unknown. We investigated the effect of recombinant ... More
Campylobacter jejuni induces maturation and cytokine production in human dendritic cells.
AuthorsHu L, Bray MD, Osorio M, Kopecko DJ,
JournalInfect Immun
PubMed ID16622206
Campylobacter jejuni is a leading bacterial cause of human diarrheal disease in both developed and developing nations. Colonic mucosal invasion and the resulting host inflammatory responses are thought to be the key contributing factors to the dysenteric form of this disease. Dendritic cells (DCs) play an important role in both ... More
Acute neuronal injury and blood genomic profiles in a nonhuman primate model for ischemic stroke.
AuthorsRodriguez-Mercado R, Ford GD, Xu Z, Kraiselburd EN, Martinez MI, Eterovic VA, Colon E, Rodriguez IV, Portilla P, Ferchmin PA, Gierbolini L, Rodriguez-Carrasquillo M, Powell MD, Pulliam JV, McCraw CO, Gates A, Ford BD,
JournalComp Med
PubMed ID23114047
The goal of this study was to characterize acute neuronal injury in a novel nonhuman primate (NHP) ischemic stroke model by using multiple outcome measures. Silk sutures were inserted into the M1 segment of the middle cerebral artery of rhesus macaques to achieve permanent occlusion of the vessel. The sutures ... More
Chronic stress, glucocorticoid receptor resistance, inflammation, and disease risk.
AuthorsCohen S, Janicki-Deverts D, Doyle WJ, Miller GE, Frank E, Rabin BS, Turner RB
JournalProc Natl Acad Sci U S A
PubMed ID22474371
We propose a model wherein chronic stress results in glucocorticoid receptor resistance (GCR) that, in turn, results in failure to down-regulate inflammatory response. Here we test the model in two viral-challenge studies. In study 1, we assessed stressful life events, GCR, and control variables including baseline antibody to the challenge ... More
Triggering of Toll-like receptor 4 expressed on human head and neck squamous cell carcinoma promotes tumor development and protects the tumor from immune attack.
AuthorsSzczepanski MJ, Czystowska M, Szajnik M, Harasymczuk M, Boyiadzis M, Kruk-Zagajewska A, Szyfter W, Zeromski J, Whiteside TL
JournalCancer Res
PubMed ID19318560
Toll-like receptors (TLR) expressed on inflammatory cells play a key role in host defense against pathogens, benefiting the host. TLR are also expressed on tumor cells. To evaluate the role of TLR in tumor cells, we investigated TLR4 signaling effects on human head and neck squamous cell carcinoma (HNSCC). Tumor ... More
Characterization of myeloid and plasmacytoid dendritic cells in human lung.
AuthorsMasten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF
JournalJ Immunol
PubMed ID17114449
Dendritic cells (DCs) are bone marrow-derived mononuclear cells that play a central role in the initiation of immune responses. Because human lung DCs have been incompletely characterized, we enumerated and phenotyped mononuclear cell populations from excess lung tissue obtained at surgery. Myeloid DCs (MDCs) were identified as CD1c(+)CD11c(+)CD14(-)HLA-DR(+) cells and ... More
Multisite comparison of high-sensitivity multiplex cytokine assays.
AuthorsBreen EC, Reynolds SM, Cox C, Jacobson LP, Magpantay L, Mulder CB, Dibben O, Margolick JB, Bream JH, Sambrano E, Martínez-Maza O, Sinclair E, Borrow P, Landay AL, Rinaldo CR, Norris PJ
JournalClin Vaccine Immunol
PubMed ID21697338
The concentrations of cytokines in human serum and plasma can provide valuable information about in vivo immune status, but low concentrations often require high-sensitivity assays to permit detection. The recent development of multiplex assays, which can measure multiple cytokines in one small sample, holds great promise, especially for studies in ... More