Simultaneous detection of multiple cytokines and chemokines from nonhuman primates using luminex technology.
AuthorsGiavedoni LD,
JournalJ Immunol Methods
PubMed ID15896800
'Cytokines and chemokines are soluble mediators of the immune system that play a crucial role in intercellular signaling, and in the recruitment of cells to inflammation sites. Identification of these molecules in nonhuman primates (NHP) is crucial for the understanding of complex physiological and pathological mechanisms that occur in these ... More
Human tumor antigen MUC1 is chemotactic for immature dendritic cells and elicits maturation but does not promote Th1 type immunity.
AuthorsCarlos CA, Dong HF, Howard OM, Oppenheim JJ, Hanisch FG, Finn OJ,
JournalJ Immunol
PubMed ID16034102
'The immunostimulatory outcome of the interactions of many pathogens with dendritic cells (DCs) has been well characterized. There are many fewer examples of similar interactions between DCs and self-molecules, especially the abnormal self-proteins such as many tumor Ags, and their effects on DC function and the immune response. We show ... More
IG20, a MADD splice variant, increases cell susceptibility to gamma-irradiation and induces soluble mediators that suppress tumor cell growth.
AuthorsEfimova E, Martinez O, Lokshin A, Arima T, Prabhakar BS,
JournalCancer Res
PubMed ID14695193
'The IG20 gene encodes at least four splice variants, including DENN-SV and IG20. DENN-SV is constitutively expressed at higher levels in tumor tissues. Cells transfected with the DENN-SV cDNA show increased resistance to tumor necrosis factor alpha (TNFalpha), TNF-related apoptosis-inducing ligand (TRAIL), etoposide, and vinblastine treatment, whereas overexpression of IG20 ... More
Doxycycline-mediated effects on persistent symptoms and systemic cytokine responses post-neuroborreliosis: a randomized, prospective, cross-over study.
AuthorsSjowall J, Ledel A, Ernerudh J, Ekerfelt C, Forsberg P,
JournalBMC Infect Dis
PubMed ID22876748
'ABSTRACT: BACKGROUND: Persistent symptoms after treatment of neuroborreliosis (NB) are well-documented, although the causative mechanisms are mainly unknown. The effect of repeated antibiotic treatment has not been studied in detail. The aim of this study was to determine whether: (1) persistent symptoms improve with doxycycline treatment; (2) doxycycline has an ... More
Analysis of SF and plasma cytokines provides insights into the mechanisms of inflammatory arthritis and may predict response to therapy.
'Biologic drugs have revolutionized the care of RA, but are expensive and not universally effective. To further understand the inflammatory mechanisms underlying RA and identify potential biomarkers predicting response to therapy, we measured multiple cytokine concentrations in SF of patients with inflammatory arthritides (IAs) and, in a subset of patients ... More
Expression of ICOS on human melanoma-infiltrating CD4+CD25highFoxp3+ T regulatory cells: implications and impact on tumor-mediated immune suppression.
'Interaction of ICOS with its ligand (ICOSL, B7-H2) promotes T cell responses. As CD4+CD25highFoxp3+ naturally occurring T regulatory cells in melanoma patients express ICOS, we investigated the impact of ICOS on naturally occurring T regulatory cell function.' ... More
Nasopharyngeal acute phase cytokines in viral upper respiratory infection: impact on acute otitis media in children.
'The role of acute phase cytokines generated in the nasopharynx during viral upper respiratory infection (URI) in subsequent development of acute otitis media (AOM) has not been examined. We studied 326 virus-positive URI episodes in 151 children aged 6-36 months. Nasopharyngeal secretions collected within 1 to 7 days of URI ... More
Cytokine and growth factor expression by HTLV-1 Lck-tax transgenic cells in SCID mice.
AuthorsWatters KM, Dean J, Hasegawa H, Sawa H, Hall W, Sheehy N,
JournalAIDS Res Hum Retroviruses
PubMed ID20438380
'The Tax protein encoded by HTLV-1 plays a key role in the development of ATL in infected individuals. Our previous studies showed that tax transgenic mice develop disease that is almost identical to human ATL, with widespread organ invasion by lymphomatous cells and the development of leukemia. The same pathology ... More
Antibodies to self-antigens predispose to primary lung allograft dysfunction and chronic rejection.
AuthorsBharat A, Saini D, Steward N, Hachem R, Trulock EP, Patterson GA, Meyers BF, Mohanakumar T
JournalAnn Thorac Surg
PubMed ID20868794
Primary graft dysfunction (PGD) is a known risk factor for bronchiolitis obliterans syndrome (BOS) after lung transplantation. Here, we report that preformed antibodies to self-antigens increase PGD risk and promote BOS. ... More
Candidate polyanion microbicides inhibit HIV-1 infection and dissemination pathways in human cervical explants.
Heterosexual intercourse remains the major route of HIV-1 transmission worldwide, with almost 5 million new infections occurring each year. Women increasingly bear a disproportionate burden of the pandemic, thus there is an urgent need to develop new strategies to reduce HIV-1 transmission that could be controlled by women themselves. The ... More
Correlation between scaffold in vivo biocompatibility and in vitro cell compatibility using mesenchymal and mononuclear cell cultures.
AuthorsSeo YK, Yoon HH, Park YS, Song KY, Lee WS, Park JK,
JournalCell Biol Toxicol
PubMed ID18946717
The aim of this study was to compare the cell compatibility of silk and polyglycolic acid (PGA) scaffolds cultured in vitro with mesenchymal stem cells (MSCs) and peripheral blood mononuclear cells (PBMCs) to their biocompatibility in vivo following implantation. Scaffolds were knitted with silk or PGA thread and the average ... More
Lactate dehydrogenase as a marker of nasopharyngeal inflammatory injury during viral upper respiratory infection: implications for acute otitis media.
AuthorsEde LC, O'Brien J, Chonmaitree T, Han Y, Patel JA,
JournalPediatr Res
PubMed ID23202721
Acute otitis media (AOM) is a frequent complication of viral upper respiratory tract infection (URI). We hypothesized that the severity of nasopharyngeal cellular injury during URI, as measured by lactate dehydrogenase (LDH) concentrations in nasopharyngeal secretions (NPSs), is related to AOM complication. LDH concentrations were determined in NPS samples (n ... More
Mild controlled hypothermia in preterm neonates with advanced necrotizing enterocolitis.
AuthorsHall NJ, Eaton S, Peters MJ, Hiorns MP, Alexander N, Azzopardi DV, Pierro A,
JournalPediatrics
PubMed ID20100756
Necrotizing enterocolitis (NEC) with multiple organ dysfunction syndrome (MODS) carries significant morbidity and mortality. There is extensive experimental evidence to support investigation of therapeutic hypothermia in infants with these conditions. We aimed to establish the feasibility and safety of mild hypothermia in preterm neonates with NEC and MODS as a ... More
Patterns of cytokine profiles differ with pregnancy outcome and ethnicity.
AuthorsVelez DR, Fortunato SJ, Morgan N, Edwards TL, Lombardi SJ, Williams SM, Menon R,
JournalHum Reprod
PubMed ID18487217
Preterm birth (PTB) is hypothesized to be an inflammatory response disease. However, no single factor alone is likely to explain PTB risk. It is more probable that coordinated networks of cytokines affect risk. Therefore, we examined the relationships between amniotic fluid (AF) cytokines/chemokines and related biomarkers in PTB and normal ... More
Bacterial infection of human hematopoietic stem cells induces monocytic differentiation.
AuthorsKolb-Mäurer A, Weissinger F, Kurzai O, Mäurer M, Wilhelm M, Goebel W,
JournalFEMS Immunol Med Microbiol
PubMed ID14987733
Differentiation of hematopoietic stem cells (HSCs) can be influenced by different stimuli, including cytotoxic agents, certain cytokines, and contact with pathogens. Infection may result in dysregulation of these important progenitor cells and therefore interfere with the availability of blood cells. In this study we analyzed the effect of bacterial infection ... More