Vaccination with adenovirus serotypes 35, 26, and 48 elicits higher levels of innate cytokine responses than adenovirus serotype 5 in rhesus monkeys.
AuthorsTeigler JE, Iampietro MJ, Barouch DH,
JournalJ Virol
PubMed ID22787208
Adenovirus (Ad) vaccine vectors have proven highly immunogenic in multiple experimental models, but the innate immune responses induced by these vectors remain poorly characterized. Here we report innate cytokine responses to 5 different Ad vectors in 26 rhesus monkeys. Vaccination with adenovirus serotype 35 (Ad35), Ad26, and Ad48 induced substantially ... More
The histone deacetylase inhibitor entinostat (SNDX-275) induces apoptosis in Hodgkin lymphoma cells and synergizes with Bcl-2 family inhibitors.
Based on promising in vitro and in vivo activity of several histone deacetylase inhibitors in Hodgkin lymphoma (HL), we investigated SNDX-275, an oral class 1 isoform-selective histone deacetylase inhibitors in HL-derived cell lines. Proliferation and cell death were examined by MTS assay, Annexin V/propidium iodide, and fluorescence-activated cell sorting analysis. Gene and ... More
Chemotherapeutic drugs and human tumor cells cytokine network.
AuthorsLevina V, Su Y, Nolen B, Liu X, Gordin Y, Lee M, Lokshin A, Gorelik E
JournalInt J Cancer
PubMed ID18697197
'The ability of human tumor cell lines to produce various cytokines, chemokines, angiogenic and growth factors was investigated using Luminex multiplex technology. Media conditioned by tumor cells protected tumor cells from drug-induced apoptosis and stimulated tumor cell proliferation. Antibodies neutralizing IL-6, CXCL8, CCL2 and CCL5 blocked this stimulation. Treatment of ... More
Sputum matrix metalloproteinase-12 in patients with chronic obstructive pulmonary disease and asthma: relationship to disease severity.
AuthorsChaudhuri R, McSharry C, Brady J, Donnelly I, Grierson C, McGuinness S, Jolly L, Weir CJ, Messow CM, Spears M, Miele G, Nocka K, Crowther D, Thompson J, Brannigan M, Lafferty J, Sproule M, Macnee W, Connell M, Murchison JT, Shepherd MC, Feuerstein G, Miller DK, Thomson NC,
JournalJ Allergy Clin Immunol
PubMed ID22305682
'Matrix metalloproteinase (MMP)-12 has been implicated in the pathogenesis of both chronic obstructive pulmonary disease (COPD) and asthma. The influence of disease severity on sputum MMP-12 concentrations and activity is not known. We sought to examine the relationship between disease severity assessed by means of lung function and computed tomography ... More
Interleukin-6 production in CD40-engaged fibrocytes in thyroid-associated ophthalmopathy: involvement of Akt and NF-?B.
AuthorsGillespie EF, Raychaudhuri N, Papageorgiou KI, Atkins SJ, Lu Y, Charara LK, Mester T, Smith TJ, Douglas RS,
JournalInvest Ophthalmol Vis Sci
PubMed ID23092922
'CD40-CD40 ligand (CD40L) interactions appear to play pathogenic roles in autoimmune disease. Here we quantify CD40 expression on fibrocytes, circulating, and bone marrow-derived progenitor cells. The functional consequences of CD40 ligation are determined since these may promote tissue remodeling linked with thyroid-associated ophthalmopathy (TAO). CD40 levels on cultivated fibrocytes and ... More
Urinary cytokine and chemokine profiles across the menstrual cycle in healthy reproductive-aged women.
'To assess the utility of urinary cytokines for monitoring reproductive function by considering detection, variation across the menstrual cycle, and relations with hormones. Longitudinal cohort study. Academic institution. Healthy, reproductive-aged women with self-reported regular menstrual cycles and at least one observed ovulatory cycle (n = 248). None. Urinary cytokines measured by 30-plex ... More
Why CCR2 and CCR5 blockade failed and why CCR1 blockade might still be effective in the treatment of rheumatoid arthritis.
AuthorsLebre MC, Vergunst CE, Choi IY, Aarrass S, Oliveira AS, Wyant T, Horuk R, Reedquist KA, Tak PP,
JournalPLoS One
PubMed ID21747955
'The aim of this study was to provide more insight into the question as to why blockade of CCR1, CCR2, and CCR5 may have failed in clinical trials in rheumatoid arthritis (RA) patients, using an in vitro monocyte migration system model. Monocytes from healthy donors (HD; n?=?8) or from RA ... More
Increased expression of TSH receptor by fibrocytes in thyroid-associated ophthalmopathy leads to chemokine production.
AuthorsGillespie EF, Papageorgiou KI, Fernando R, Raychaudhuri N, Cockerham KP, Charara LK, Goncalves AC, Zhao SX, Ginter A, Lu Y, Smith TJ, Douglas RS,
JournalJ Clin Endocrinol Metab
PubMed ID22399514
'The molecular basis for anatomically dispersed clinical manifestations in Graves'' disease (GD) eludes our understanding. Bone marrow-derived, pluripotent fibrocytes represent a subset of peripheral blood mononuclear cells and infiltrate the orbital and thyroid tissues in GD. These cells may be involved in the pathogenesis of thyroid-associated ophthalmopathy (TAO). The objective ... More
Differential susceptibility of peripheral blood CD5+ and CD5- B cells to apoptosis in chronic hepatitis C patients.
AuthorsMizuochi T, Ito M, Takai K, Yamaguchi K,
JournalBiochem Biophys Res Commun
PubMed ID19737536
'A body of evidence has suggested a close link between chronic hepatitis C virus (HCV) infection and B cell abnormalities, including mixed cryoglobulinemia, rheumatoid factor (RF) production, and lymphoproliferative disorders that may develop into non-Hodgkin''s lymphoma. Recent studies have demonstrated the expansion of CD5(+) B cells in the peripheral blood ... More
Phase 2 study of dasatinib in the treatment of head and neck squamous cell carcinoma.
AuthorsBrooks HD, Glisson BS, Bekele BN, Johnson FM, Ginsberg LE, El-Naggar A, Culotta KS, Takebe N, Wright J, Tran HT, Papadimitrakopoulou VA,
JournalCancer
PubMed ID21523723
'Treatment options for patients with advanced head and neck squamous cell carcinoma (HNSCC) are scarce. This phase 2 study was conducted to evaluate the safety, tolerability, pharmacokinetics, and efficacy of dasatinib in this setting. Patients with recurrent and/or metastatic HNSCC after platinum-based therapy were treated with dasatinib either orally or ... More
Local and systemic pro-inflammatory and anti-inflammatory cytokine patterns in patients with chronic subdural hematoma: a prospective study.
AuthorsStanisic M, Aasen AO, Pripp AH, Lindegaard KF, Ramm-Pettersen J, Lyngstadaas SP, Ivanovic J, Konglund A, Ilstad E, Sandell T, Ellingsen O, Sæhle T,
JournalInflamm Res
PubMed ID22527446
'Innate immune pro- and anti-inflammatory responses in patients with chronic subdural hematoma (CSDH) were investigated by measuring and comparing the systemic and subdural fluid levels of cytokines. Cytokine values were analyzed in samples obtained during surgery of 56 adult patients who were operated on for unilateral CSDHs using a Multiplex ... More
Role of eotaxin-1 signaling in ovarian cancer.
AuthorsLevina V, Nolen BM, Marrangoni AM, Cheng P, Marks JR, Szczepanski MJ, Szajnik ME, Gorelik E, Lokshin AE,
JournalClin Cancer Res
PubMed ID19351767
'Tumor cell growth and migration can be directly regulated by chemokines. In the present study, the association of CCL11 with ovarian cancer has been investigated. Experimental Design and Circulating levels of CCL11 in sera of patients with ovarian cancer were significantly lower than those in healthy women or women with ... More
The effect of topical amphotericin B on inflammatory markers in patients with chronic rhinosinusitis: a multicenter randomized controlled study.
AuthorsEbbens FA, Georgalas C, Luiten S, van Drunen CM, Badia L, Scadding GK, Hellings PW, Jorissen M, Mullol J, Cardesin A, Bachert C, van Zele TP, Lund VJ, Fokkens WJ,
JournalLaryngoscope
PubMed ID19160404
'It has been suggested that an exaggerated immune response to fungi is crucial in the pathogenesis of chronic rhinosinusitis (CRS). Based on this rationale, the use of topical antifungals (amphotericin B) has been advocated. Studies on its clinical effectiveness are, however, contradictory. To examine the effect of nasal antifungal treatment ... More
Temporal reliability of cytokines and growth factors in EDTA plasma.
AuthorsClendenen TV, Arslan AA, Lokshin AE, Idahl A, Hallmans G, Koenig KL, Marrangoni AM, Nolen BM, Ohlson N, Zeleniuch-Jacquotte A, Lundin E
JournalBMC Res Notes
PubMed ID21073739
Cytokines are involved in the development of chronic diseases, including cancer. It is important to evaluate the temporal reproducibility of cytokines in plasma prior to conducting epidemiologic studies utilizing these markers. ... More
Multiplex analysis of serum cytokines in melanoma patients treated with interferon-alpha2b.
Interferon (IFN)-alpha2b is the only Food and Drug Administration-approved treatment for operable high-risk melanoma that has been shown to significantly and durably prolong relapse-free survival (RFS) of patients with stage IIB-III melanoma. Development of reliable serum assays may contribute to the development of methods for earlier detection of melanoma and ... More
The effect of lung cancer on cytokine expression in peripheral blood mononuclear cells.
The purpose of this study is to evaluate cytokine expression by peripheral blood mononuclear cells (PBMC) from stage I lung cancer patients and to confirm these expression patterns by exposing PBMCs to lung cancer cells in vitro. Five altered cytokines in stage I lung cancer patients (CCL3, IL8, IL1ß, CXCL10, ... More
Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients.
AuthorsSrivastava RM, Lee SC, Andrade Filho PA, Lord CA, Jie HB, Davidson HC, López-Albaitero A, Gibson SP, Gooding WE, Ferrone S, Ferris RL,
JournalClin Cancer Res
PubMed ID23444227
Tumor antigen-specific monoclonal antibodies (mAb) block oncogenic signaling and induce Fc? receptor (Fc?R)-mediated cytotoxicity. However, the role of CD8(+) CTL and Fc?R in initiating innate and adaptive immune responses in mAb-treated human patients with cancer is still emerging. Fc?RIIIa codon 158 polymorphism was correlated with survival in 107 cetuximab-treated patients ... More
Possible recruitment of peripheral blood CXCR3+ CD27+ CD19+ B cells to the liver of chronic hepatitis C patients.
AuthorsMizuochi T, Ito M, Saito K, Kasai M, Kunimura T, Morohoshi T, Momose H, Hamaguchi I, Takai K, Iino S, Suzuki M, Mochida S, Ikebuchi K, Yamaguchi K,
JournalJ Interferon Cytokine Res
PubMed ID20377416
It has been suggested that hepatitis C virus (HCV) infects not only hepatocytes but also immune cells, including B cells. HCV infection of B cells is the likely cause of B-cell dysregulation disorders such as mixed cryoglobulinemia, rheumatoid factor production, and B-cell lymphoproliferative disorders that may evolve into non-Hodgkin's lymphoma. ... More
Distinct patterns of cytokine and angiogenic factor modulation and markers of benefit for vandetanib and/or chemotherapy in patients with non-small-cell lung cancer.
AuthorsHanrahan EO, Lin HY, Kim ES, Yan S, Du DZ, McKee KS, Tran HT, Lee JJ, Ryan AJ, Langmuir P, Johnson BE, Heymach JV,
JournalJ Clin Oncol
PubMed ID19949019
There is an unmet need for biomarkers for identifying patients likely to benefit from anticancer treatments, selecting dose, and understanding mechanisms of resistance. Plasma vascular endothelial growth factor (VEGF) and soluble VEGF receptor 2 (sVEGFR-2) are known to be modulated by VEGF pathway inhibitors. It is unknown whether chemotherapy or ... More
Were monocytes responsible for initiating the cytokine storm in the TGN1412 clinical trial tragedy?
AuthorsSandilands GP, Wilson M, Huser C, Jolly L, Sands WA, McSharry C,
JournalClin Exp Immunol
PubMed ID20964641
The precise biological mechanisms that caused the TGN1412 clinical trial tragedy (also known as 'The Elephant Man Clinical Trial') in March 2006 remain a mystery to this day. It is assumed widely that the drug used in this trial (TGN1412) bound to CD28 on T lymphocytes and following activation of ... More
Reduced FcepsilonRI-mediated release of asthma-promoting cytokines and chemokines from human basophils during omalizumab therapy.
AuthorsOliver JM, Tarleton CA, Gilmartin L, Archibeque T, Qualls CR, Diehl L, Wilson BS, Schuyler M,
JournalInt Arch Allergy Immunol
PubMed ID19844128
Treating asthmatics with the humanized IgE-scavenging antibody, omalizumab (rhuMAb-E25, Xolair, reduces airways inflammation and asthma symptoms. Previously, omalizumab was shown to cause a dramatic and reversible loss of cell surface high-affinity IgE receptors, FcepsilonRI, from the peripheral blood basophils of asthmatics. The consequences of receptor loss for the FcepsilonRI-mediated synthesis ... More