Lipofectamine™ MessengerMAX™ 转染试剂

Name: Lipofectamine™ MessengerMAX™ 转染试剂
SKU: LMRNA008
Price: 8618.00 CNY

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货号	数量
LMRNA008	0.75 mL
LMRNA001	0.1 mL
LMRNA003	0.3 mL
LMRNA015	1.5 mL
LMRNA150	15 mL

5 选项

货号 LMRNA008

价格（CNY)

8,618.00

Each

添加至购物车

数量:

0.75 mL

价格（CNY)

8,618.00

Each

添加至购物车

Lipofectamine™ MessengerMAX mRNA 转染试剂可在神经元及广泛的原代细胞中实现出色的转染效率，从而改善应用结果以及更具生物学相关性的研究。这是因为我们新型的脂质纳米微粒技术经过优化，能够在无使用 DNA 所需的入核步骤的情况下递送较高量的 mRNA。

使用以下试剂成功转染更聚预测性的细胞模型：
•在神经元和原代细胞类型中表现惊人的转染效率
•更快的蛋白表达，无基因组整合风险
•切割率高达使用 mRNA CRISPR 的10倍

在新型基因组编辑应用中实现高转染效率
Lipofectamine™ MessengerMAX™ 试剂有助于增加利用 GeneArt™ CRISPR 核酸酶 mRNA 通过高效转染成功切割和重组的可能性，最终实现基因修饰效率较大化以及简化下游过程。

高效转染难转染细胞
Lipofectamine™ MessengerMAX™ 试剂设计用于转染更高量的 mRNA 到神经元以及广泛类型的难转染原代细胞中。这使得无需电穿孔或病毒，与其他基于脂质的试剂相比，转染效率提高了 >2 倍。

利用 mRNA 转染的优势
转染 mRNA 不需要细胞核摄取，其结果比 DNA 转染更快表达蛋白，因为 mRNA 在细胞质中被翻译。此外，mRNA 不存在基因组整合风险，转染效率也不依赖于细胞周期。

仅供科研使用。不可用于诊断程序。

规格

适用于（应用）转染

环保功能绿色可持续包装

产品线Lipofectamine™

产品类型转染试剂

数量0.75 mL

血清兼容性是

运输条件室温

细胞类型已建立的细胞系, 神经元细胞, 干细胞, 原代细胞, 难转染细胞

产品规格6孔板、12孔板、24孔板、48孔板、96孔板、培养瓶

样品类型mRNA

转染技术脂质转染

Unit SizeEach

内容与储存

储存在冰箱 (2–8°C) 中。

常见问题解答 (FAQ)

Can I deliver single-stranded oligonucleotides with Lipofectamine MessengerMAX Transfection Reagent?

If you are delivering a single-stranded oligonucleotide on its own, we recommend using Lipofectamine RNAiMAX Transfection Reagent. However, Lipofectamine MessengerMAX Transfection Reagent can be used for genome editing purposes when Cas9 mRNA is co-delivered with a sgRNA and a single-stranded or double-stranded donor template.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How do I use Lipofectamine MessengerMAX Reagent to deliver both my GeneArt Cas9 mRNA and IVT gRNA together?

Lipofectamine MessengerMAX Reagent may be used for both single gRNA delivery or multiplexing (gRNA for multiple targets) purposes with high transfection efficiency when used with GeneArt Cas9 mRNA. For a detailed protocol, please refer to the GeneArt Cas9 mRNA manual (https://www.thermofisher.com/order/catalog/product/A25640?ICID=search-a25640). We recommend using either the Invitrogen GeneArt Genomic Cleavage Detection Kit (Cat. No. A24732) or Invitrogen GeneArt Genomic Cleavage Selection Kit (Cat. No. A27663) for mutant analysis.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Which cell types can be used with Lipofectamine MessengerMAX Transfection Reagent?

Lipofectamine MessengerMAX Reagent demonstrates low toxicity and high transfection efficiency for all cell types (easy or difficult-to-transfect, primary, and stem cells).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

When would I use Lipofectamine MessengerMAX Reagent over other transfection reagents?

Lipofectamine MessengerMAX Regent is an excellent reagent for co-delivery of Invitrogen GeneArt CRISPR Cas9 mRNA with in vitro transcribed gRNA for geneome editing purposes. Lipofectamine MessengerMAX Reagent has the added benefit of flexibly delivering short dsDNA or HDR templates (0.5-1 kb) which can be ordered through the Invitrogen GeneArt Strings services.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the Invitrogen Lipofectamine MessengerMAX Transfection Reagent?

Lipofectamine MessengerMAX Transfection Reagent is an animal origin-free transfection reagent, especially formulated for the delivery of mRNA, small RNA (eg.,CRISPR IVT gRNA, siRNA, or miRNA), and short dsDNA or HDR templates (0.5-1 kb). Lipofectamine MessengerMAX Reagent is an excellent reagent choice for CRISPR-mediated genome editing applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.