Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes.
AuthorsSun WC, Gee KR, Haugland RP
JournalBioorg Med Chem Lett
PubMed ID9873685
'Two new fluorinated fluorescent dyes, 6,8-difluoro-7-hydroxy-4-methylcoumarin (Marina Blue) and 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue), exhibit excellent photophysical properties among a series of novel fluorinated 7-hydroxycoumarins. Most of these fluorinated coumarins have quantum yields (0.63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their ... More
Optically directed molecular transport and 3D isoelectric positioning of amphoteric biomolecules.
AuthorsHafeman DG, Harkins JB, Witkowski CE, Lewis NS, Warmack RJ, Brown GM, Thundat T
JournalProc Natl Acad Sci U S A
PubMed ID16618926
'We demonstrate the formation of charged molecular packets and their transport within optically created electrical force-field traps in a pH-buffered electrolyte. We call this process photoelectrophoretic localization and transport (PELT). The electrolyte is in contact with a photoconductive semiconductor electrode and a counterelectrode that are connected through an external circuit. ... More
Fluorescent labeling of cell-free synthesized proteins by incorporation of fluorophore-conjugated nonnatural amino acids.
AuthorsKang SH, Jun SY, Kim DM
JournalAnal Biochem
PubMed ID17113028
'Although fluorescent dyes, such as fluorescein derivatives, have bulky and complex structures, nonnatural amino acids carrying these fluorescein derivatives are acceptable by the Escherichia coli ribosome and are useful for the cotranslational fluorescent labeling of cell-free synthesized proteins. Surprisingly, the incorporation efficiency of nonnatural amino acids carrying fluorescein derivatives into ... More
Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp.
AuthorsO'Brien RJ, Xu D, Petralia RS, Steward O, Huganir RL, Worley P
JournalNeuron
PubMed ID10399937
Narp (neuronal activity-regulated pentraxin) is a secreted immediate-early gene (IEG) regulated by synaptic activity in brain. In this study, we demonstrate that Narp possesses several properties that make it likely to play a key role in excitatory synaptogenesis. Narp is shown to be selectively enriched at excitatory synapses on neurons ... More
Investigation of domain formation in sphingomyelin/cholesterol/POPC mixtures by fluorescence resonance energy transfer and Monte Carlo simulations.
AuthorsFrazier ML, Wright JR, Pokorny A, Almeida PF
JournalBiophys J
PubMed ID17218467
We have recently proposed a phase diagram for mixtures of porcine brain sphingomyelin (BSM), cholesterol (Chol), and 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) on the basis of kinetics of carboxyfluorescein efflux induced by the amphipathic peptide delta-lysin. Although that study indicated the existence of domains, phase separations in the micrometer scale have not been ... More