Integration of multianalyte sensing functions on a capillary-assembled microchip: simultaneous determination of ion concentrations and enzymatic activities by a "drop-and-sip" technique.
AuthorsHenares TG, Takaishi M, Yoshida N, Terabe S, Mizutani F, Sekizawa R, Hisamoto H
JournalAnal Chem
PubMed ID17263315
'A general and simple implementation of simultaneous multiparametric sensing in a single microchip is presented by using a capillary-assembled microchip (CAs-CHIP) integrated with the plural different reagent-release capillaries (RRCs), acting as various biochemical sensors. A novel "drop-and-sip" technique of fluid handling is performed with a microliter droplet of a model ... More
Measurement and simulation of myoplasmic calcium transients in mouse slow-twitch muscle fibres.
AuthorsHollingworth S, Kim MM, Baylor SM,
JournalJ Physiol
PubMed ID22124146
'Bundles of intact fibres from soleus muscles of adult mice were isolated by dissection and one fibre within a bundle was micro-injected with either furaptra or mag-fluo-4, two low-affinity rapidly responding Ca(2+) indicators. Fibres were activated by action potentials to elicit changes in indicator fluorescence (?F), a monitor of the ... More
The endoplasmic reticulum: one continuous or several separate Ca(2+) stores?
AuthorsPetersen OH, Tepikin A, Park MK
JournalTrends Neurosci
PubMed ID11311379
The Ca2+ store and sink in the endoplasmic reticulum (ER) is important for Ca2+ signal integration and for conveyance of information in spatial and temporal domains. Textbooks regard the ER as one continuous network, but biochemical and biophysical studies revealed apparently discrete ER Ca2+ stores. Recent direct studies of ER ... More
Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.
AuthorsHollingworth S, Gee KR, Baylor SM,
JournalBiophys J
PubMed ID19804716
The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of the above indicators and, usually, furaptra (previously shown to rapidly ... More
Correlations between the functional integrity of the endoplasmic reticulum and polarized Ca2+ signalling in mouse lacrimal acinar cells: a role for inositol 1,3,4,5-tetrakisphosphate.
AuthorsHarmer AR, Gallacher DV, Smith PM
JournalBiochem J
PubMed ID12117415
Ca(2+) signalling in exocrine acinar cells has been shown to be both polarized and pulsatile in all cell types tested, except acutely isolated mouse lacrimal acinar cells. Lacrimal cells are also unusual in that they display a very low sensitivity to Ins(1,4,5) P (3) (Ins P (3)) that may be ... More