用于流式细胞分析的 MitoProbe™ TMRM 检测试剂盒
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用于流式细胞分析的 MitoProbe™ TMRM 检测试剂盒
Thermo Scientific™

用于流式细胞分析的 MitoProbe™ TMRM 检测试剂盒

The MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCP了解更多信息
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货号数量
M20036100 assays
货号 M20036
价格（CNY)
2,733.00
飞享价
Ends: 31-Dec-2025
3,631.00
共减 898.00 (25%)
Each
添加至购物车
数量:
100 assays
价格（CNY)
2,733.00
飞享价
Ends: 31-Dec-2025
3,631.00
共减 898.00 (25%)
Each
添加至购物车
The MitoProbe TMRM Assay Kit contains tetramethylrhodamine methyl ester (TMRM) for the detection of mitochondrial membrane potential state and CCCP for the induction of mitochondrial membrane depolarization, if desired.

• Study mitochondrial membrane potential in live cells
• Quick, easy protocol

Determining the state of mitochondrial health can be achieved by detecting changes in the mitochondrial membrane potential. In a healthy cell with active mitochondria, TMRM is readily sequestered, thereby emitting a red-orange fluorescent signal. When apoptosis is induced or a treatment like CCCP is applied, the mitochondrial membrane is depolarized and the TMRM signal diminishes.

规格
检测方法荧光
产品规格管装
数量100 assays
运输条件湿冰
发射561
适用于（应用）TMRM 检测
适用于（设备）流式细胞仪
产品类型流式细胞分析测定试剂盒
Unit SizeEach
内容与储存
  • 1小瓶 TMRM（100次检测）
  • 1小瓶 50 mM CCCP
  • 保存在 -5°C 到 -30°C。

常见问题解答 (FAQ)

In the MitoProbe TMRM Assay Kit for Flow Cytometry, should CCCP be present when adding the TMRM?

Yes. The CCCP should be first added to the cell sample, incubated for 5 mins, and then the TMRM should be added and incubated for another 30 mins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How does the MitoProbe TMRM Assay Kit for Flow Cytometry (Cat. No.M20036) differ from the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is provided as a 100 µM solution of TMRM in DMSO (5 vials, 100 µL in each vial). The MitoProbe TMRM Assay Kit for Flow Cytometry provides the TMRM as a 20 µM stock solution in DMSO (1 vial, 100 µL) and a vial of CCCP. Both kits have the stock solutions as a 1000X concentration, but final working concentrations required for imaging applications are 5-fold higher than what is needed for flow cytometry analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.