Superoxide production in the islet of Langerhans detected by the MCLA chemiluminescence method.
AuthorsSakurai T, Terakawa S
JournalMethods Mol Biol
PubMed ID12152200
Generation of superoxide anion by succinate-cytochrome c reductase from bovine heart mitochondria.
AuthorsZhang L, Yu L, Yu CA
JournalJ Biol Chem
PubMed ID9852050
'Production of superoxide anion (O-2), measured as the chemiluminescence of the 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]pyrazin-3-one hydrochloride (MCLA)-O-2 adduct, was observed during electron transfer from succinate to cytochrome c by reconstituted succinate-cytochrome c reductase-phospholipid vesicles replenished with succinate dehydrogenase. Addition of carbonyl cyanide p-trifluoromethoxyphenylhydrazone or detergent to the reconstituted reductase-phospholipid vesicles abolished O-2 ... More
Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012.
AuthorsImada I, Sato EF, Miyamoto M, Ichimori Y, Minamiyama Y, Konaka R, Inoue M
JournalAnal Biochem
PubMed ID10361004
'Reactive oxygen species (ROS) play important roles in the defense mechanism against infection and in the pathogenesis of various diseases. Although chemical properties of ROS generated by leukocytes have been studied extensively, methods available for their analysis are not sufficiently sensitive. We found that 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4-(2H,3H)dione (L-012) reacted with various types ... More
Superoxide anion impairs contractility in cultured aortic smooth muscle cells.
AuthorsKimura C, Cheng W, Hisadome K, Wang YP, Koyama T, Karashima Y, Oike M, Ito Y
JournalAm J Physiol Heart Circ Physiol
PubMed ID12063312
'We examined the effects of superoxide anion (O) generated by xanthine plus xanthine oxidase (X/XO) on the intracellular Ca(2+) concentration ([Ca(2+)](i)) and muscle contractility in cultured bovine aortic smooth muscle cells (BASMC). Cells were grown on collagen-coated dish for the measurement of [Ca(2+)](i). Pretreatment with X/XO inhibited ATP-induced Ca(2+) transient ... More
The involvement and sources of active oxygen in experimentally induced acute pancreatitis.
AuthorsIto T, Nakao A, Kishimoto W, Nakano M, Takagi H
JournalPancreas
PubMed ID8720665
'The involvement of active oxygen has been suggested in the development of cerulein-induced acute pancreatitis in rats. Previously, we directly detected pancreatic active oxygen (O2-) production in rats with cerulein-induced pancreatitis by using a supersensitive photon counter and a cypridina luciferin analogue (MCLA) that reacts specifically with O2- by emitting ... More
Improved preparation of leukocytes for chemiluminescent study of human phagocytic leukocyte-generated reactive oxygen species.
AuthorsSaniabadi AR, Nakano M
JournalJ Biolumin Chemilumin
PubMed ID8396845
'Leukocyte oxidative function was investigated in a more physiological milieu than currently used in the chemiluminescence (CL) technique. Heparinized blood was mixed with 6% dextran-T70 (9:1) and the leukocyte-rich plasma obtained without centrifugation was used for the CL experiments (phagocyte count was adjusted to 0.7 x 10(6)/mL with Hanks'' buffer) ... More
Evaluation of five imidazopyrazinone-type chemiluminescent superoxide probes and their application to the measurement of superoxide anion generated by Listeria monocytogenes.
AuthorsShimomura O, Wu C, Murai A, Nakamura H
JournalAnal Biochem
PubMed ID9570834
'Superoxide-triggered chemiluminescence of five new imidazopyrazinone derivatives was investigated using the hypoxanthine-xanthine oxidase system as the source of superoxide anion. The results showed that they are highly sensitive and have favorable properties in measuring superoxide anion. With those new probes, the generation of superoxide anion from the bacteria Listeria monocytogenes ... More
Continuous detection of superoxide in situ during ischemia and reperfusion in the rabbit heart.
AuthorsUshiroda S, Maruyama Y, Nakano M
JournalJpn Heart J
PubMed ID9186285
'In order to clarify the time course of superoxide generation in situ during ischemia and reperfusion in the rabbit heart, we used a method of enhanced chemiluminescence (CL) with 2-methyl-6-[p-methoxyphenyl]-3, 7-dihydroimidazo [1, 2-alpha]pyrazin-3-one (MCLA) as a specific probe for detecting superoxide radicals. The surface of the rabbit heart was exposed ... More
A highly sensitive method for determining both Mn- and Cu-Zn superoxide dismutase activities in tissues and blood cells.
AuthorsNakano M, Kimura H, Hara M, Kuroiwa M, Kato M, Totsune K, Yoshikawa T
JournalAnal Biochem
PubMed ID2200307
'A highly sensitive method for determining the superoxide dismutase (EC 1.15.1.1) in various tissues and blood cells is described. This method involves inhibition of a cypridina luciferin analog that is chemiluminescence dependent upon O2- generated by hypoxanthine-xanthine oxidase. Manganeous superoxide dismutase, which is sensitive to sodium dodecyl sulfate, was determined ... More
A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells.
AuthorsNishinaka Y, Aramaki Y, Yoshida H, Masuya H, Sugawara T, Ichimori Y
JournalBiochem Biophys Res Commun
PubMed ID8390246
'A sensitive chemiluminescence method for measuring the production of superoxide anion (O2-) by activated EoL-1 cells (human eosinophilic leukemia cell line) is described. Recently, we succeeded in synthesizing a new chemiluminescence probe, 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)dione (L-012). In the presence of L-012, activated EoL-1 cells which produce reactive oxygen species generated a marked ... More
The first application of a chemiluminescence probe, 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA), for detecting O2- production, in vitro, from Kupffer cells stimulated by phorbol myristate acetate.
AuthorsUehara K, Maruyama N, Huang CK, Nakano M
JournalFEBS Lett
PubMed ID8253189
'The purpose of this study was to investigate the generation of superoxide anion radical, O2-, by Kupffer cells in vivo in rat liver. Phorbol myristate acetate (PMA) was infused into perfused rat liver which had been continuously infused with 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo- [1,2-a]pyrazin-3-one (MCLA, a highly sensitive luminescence reagent for O2-) and ... More
Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1.
AuthorsChistoserdova L, Lidstrom ME
JournalMicrobiology
PubMed ID9168622
'A region of 14.2 kb has been analysed that is a part of a locus on the Methylobacterium extorquens AM1 chromosome containing a number of genes involved in one-carbon (C1) metabolism, including serine cycle genes, pqq genes, regulatory methanol oxidation genes and the gene for N5,N10-methylene tetrahydrofolate dehydrogenase (mtdA). Fifteen ... More
Enhanced chemiluminescence of 6-(4-methoxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one by attachment of a cyclomaltooligosaccharide (cyclodextrin). Attachment of cyclomaltononaose (delta-cyclodextrin).
AuthorsTeranishi K, Nishiguchi T, Ueda H
JournalCarbohydr Res
PubMed ID12681923
'2-Methyl-6-(4-methoxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one (MCLA) is an oxygen-induced chemiluminescent compound. It has been shown that the chemiluminescence can be enhanced by forming a cyclomaltooligosaccharide (cyclodextrin)-bound MCLA, and therefore, in continuation of the survey of the types of cyclodextrins, in this study, MCLA was attached to the secondary hydroxyl face of delta-cyclodextrin, which consists ... More
Effects of antiallergic drugs on superoxide anion generation from activated human alveolar macrophages measured by chemiluminescence method.
AuthorsKurosawa M, Ishizuka T, Kobayashi S, Nakano M
JournalArzneimittelforschung
PubMed ID1675572
'Human alveolar macrophages were recovered by bronchoalveolar lavage and superoxide anion (O2-) generation from the macrophages were measured by 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA)-dependent luminescence. Addition of 0.5 mumol/l MCLA and a stimulatory agent, such as phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP), to a suspension of the cells caused a marked ... More
Superoxide production from paraquat evoked by exogenous NADPH in pulmonary endothelial cells.
AuthorsTampo Y, Tsukamoto M, Yonaha M
JournalFree Radic Biol Med
PubMed ID10490279
'Superoxide production from paraquat in a pulmonary microvascular endothelial cell (PMEC) suspension was demonstrated using 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyraz in-3-one (MCLA), a chemiluminescence probe, to detect superoxide anions. Increased rates of superoxide production from paraquat, which were sensitive to superoxide dismutase (SOD), required the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) in ... More
A possible mechanism of iron neurotoxicity.
AuthorsNakano M
JournalEur Neurol
PubMed ID8375432
'Iron-dependent microsomal lipid peroxidation and catechol-iron (copper)-induced lipid peroxidation have been studied to prove possible nigrostriatal cell damage. Iron-dependent microsomal lipid peroxidation could be initiated by reduced irons coordinated with phosphate moieties in the membranes and significantly inhibited by copper salicylate (hydrophobic and permeable O2-scavenger) and desferrioxamine (a powerful iron-chelating ... More
Detection of heterogeneity of Cu, Zn-superoxide dismutase with monoclonal antibodies and the establishment of a highly sensitive fluorescence sandwich enzyme-linked immunosorbent assay.
AuthorsOno K, Kimura S, Nakano M, Naruse T
JournalFEBS Lett
PubMed ID2026245
'Murine monoclonal antibodies directed against a native form of Cu, Zn-superoxide dismutase (SOD) were produced by immunizing SOD purified from human erythrocytes. The monoclonal antibodies able to bind SOD were further screened for their ability to absorb SOD activity using anti-mouse IgG conjugated iron beads as solid supports in magnetic ... More
Serum of Behçet's disease enhances superoxide production of normal neutrophils.
AuthorsYoshida T, Tanaka M, Sotomatsu A, Okamoto K, Hirai S
JournalFree Radic Res
PubMed ID9554831
'Using an MCLA-dependent chemiluminescence technique, we evaluated superoxide production by neutrophils isolated from 7 patients with Behçet''s disease. After stimulation by phorbol myristate acetate, N-formyl-methionyl-leucyl-phenylalanine or opsonized zymosan, neutrophils from the patients produced significantly more superoxide than those from 20 controls. Pretreatment of control neutrophils with serum prominently enhanced superoxide ... More
A defect in the cytochrome b large subunit in complex II causes both superoxide anion overproduction and abnormal energy metabolism in Caenorhabditis elegans.
AuthorsSenoo-Matsuda N, Yasuda K, Tsuda M, Ohkubo T, Yoshimura S, Nakazawa H, Hartman PS, Ishii N
JournalJ Biol Chem
PubMed ID11527963
'A mev-1(kn1) mutant of the nematode Caenorhabditis elegans is defective in the cytochrome b large subunit (Cyt-1/ceSDHC) in complex II of the mitochondrial electron transport chain. We have previously shown that a mutation in mev-1 causes shortened life span and rapid accumulation of aging markers such as fluorescent materials and ... More
Hypoxia-induced alterations in Ca(2+) mobilization in brain microvascular endothelial cells.
AuthorsKimura C, Oike M, Ito Y
JournalAm J Physiol Heart Circ Physiol
PubMed ID11045967
'To investigate the possible cellular mechanisms of the ischemia-induced impairments of cerebral microcirculation, we investigated the effects of hypoxia/reoxygenation on the intracellular Ca(2+) concentration ([Ca(2+)](i)) in bovine brain microvascular endothelial cells (BBEC). In the cells kept in normal air, ATP elicited Ca(2+) oscillations in a concentration-dependent manner. When the cells ... More
Developmental expression of copper, zinc-superoxide dismutase in human brain by chemiluminescence.
AuthorsNishida A, Misaki Y, Kuruta H, Takashima S
JournalBrain Dev
PubMed ID8059927
'Copper, zinc-superoxide dismutase (Cu,Zn-SOD) activities were determined by the chemiluminescence method using the cypridina luciferin analog (MCLA) in 22 human brains from fetuses to adults. Cu,Zn-SOD activity of the cerebral cortex and white matter increased from 15% in fetuses to 50% of adult levels in neonates. The activity of the ... More
Low superoxide scavenging activity associated with enhanced superoxide generation by monocytes from male hypertriglyceridemia with and without diabetes.
AuthorsPrónai L, Hiramatsu K, Saigusa Y, Nakazawa H
JournalAtherosclerosis
PubMed ID1665975
'To investigate the mechanism of increased superoxide (O2-) generation by monocytes from patients with hypertriglyceridemia, superoxide scavenging activity (SSA) and O2- generation by monocytes were determined concomitantly employing an electron spin resonance/spin trapping method and 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo [1,2-a]-pyrazin-3-one (MCLA)-dependent chemiluminescence, respectively. Peripheral monocytes were separated by the adherent methods from the ... More
Time course of superoxide generation by leukocytes--the MCLA chemiluminescence system.
AuthorsPrónai L, Nakazawa H, Ichimori K, Saigusa Y, Ohkubo T, Hiramatsu K, Arimori S, Fehér J
JournalInflammation
PubMed ID1330925
'This study was performed to examine the pattern of superoxide (O2-.) generation from leukocytes using the O2-. specific chemiluminescence (CL) method. Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo [1,2-alpha]pyrazin-3-one (MCLA) was used as a CL probe. The appropriate conditions of the MCLA method was first determined for the evaluation of the time course ... More
Effect of cystathionine as a scavenger of superoxide generated from human leukocytes or derived from xanthine oxidase in vitro.
AuthorsWada K, Kamisaki Y, Nakamoto K, Itoh T
JournalEur J Pharmacol
PubMed ID8904086
'We studied the direct effects of cystathionine on human leukocyte-generated or xanthine-xanthine oxidase-derived superoxide radicals in vitro. Washed leukocyte suspensions (10(6) cells/ml) prepared from healthy male volunteers were stimulated with phorbol myristate acetate (1 mu M) or opsonized zymosan (1 mg/ml) to generate superoxide radicals, which were measured with a ... More
Failure of inhibitory effects by platelets on super-oxide anion generation from stimulated neutrophils in a severe bronchial asthmatic.
AuthorsKurosawa M, Kobayashi H, Kobayashi S, Nakano M
JournalAllergy
PubMed ID1647708
'Blood was drawn from healthy human volunteers and a 30-year-old woman with severe bronchial asthma (atopic and perennial type), whose peripheral blood platelets counts were continuously higher than 60 x 10(4)/mm3. Neutrophils were purified on a Conray-Ficoll gradient and platelets were isolated by a gel filtration method with Sepharose 2B. ... More
Detection of superoxide free radicals in rats with acute pancreatitis.
AuthorsKishimoto W, Nakao A, Nakano M, Takahashi A, Inaba H, Takagi H
JournalPancreas
PubMed ID7479667
'To study the importance of oxygen-derived free radicals in acute pancreatitis, an experimental study of in vivo detection of superoxide free radicals (O2-) was performed using rats. Using a new chemiluminescence probe (2-methyl-6-[p-methoxyphenyl]-3,7-dihydro-imidazol[1,2-1]pyrazin- 3-one; MCLA; a Cypridina luciferin analogue) and a highly sensitive photon counting system, O2- from the pancreatic ... More
Measurement of rate constants for quenching singlet oxygen with a Cypridina luciferin analog (2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo [1,2-a]pyrazin-3-one) and sodium azide.
AuthorsMashiko S, Suzuki N, Koga S, Nakano M, Goto T, Ashino T, Mizumoto I, Inaba H
JournalJ Biolumin Chemilumin
PubMed ID1882708
'The rate constants for [1O2] [MCLA] and [1O2] [NaN3] were measured by quenching the near-infrared emission (1 delta g----3 sigma g) in steady state with MCLA and NaN3, respectively. 1O2 was constantly generated by energy transfer to O2 from Ar laser-excited Rose Bengal. The Stern-Volmer plots yielded the second-order rate ... More
The first observation of O2- generation at real time in vivo from non-Kupffer sinusoidal cells in perfused rat liver during acute ethanol intoxication.
AuthorsNakano M, Kikuyama M, Hasegawa T, Ito T, Sakurai K, Hiraishi K, Hashimura E, Adachi M
JournalFEBS Lett
PubMed ID7556655
'Infusion of ethanol or phorbol myristate acetate (PMA) into the perfused rat liver immediately produces O2- which was detected directly by infusion of a Cypridina luciferin analogue, MCLA as a chemiluminescence reagent. The MCLA photon emission was inhibitable by SOD. Generation of O2- in the liver was further verified by ... More
A miniaturized fibrinolytic assay for plasminogen activators.
AuthorsLewis ML, Nachtwey DS, Damron KL
JournalThromb Res
PubMed ID1811341
'This report describes a micro-clot lysis assay (MCLA) for evaluating fibrinolytic activity of plasminogen activators (PA). Fibrin clots were formed in wells of microtiter plates. Lysis of the clots by PA, indicated by change in turbidity (optical density, OD), was monitored with a microplate reader at five minutes intervals. Log-log ... More
Highly sensitive chemiluminescence method for determining myeloperoxidase in human polymorphonuclear leukocytes.
AuthorsUehara K, Hori K, Nakano M, Koga S
JournalAnal Biochem
PubMed ID1667454
'Myeloperoxidase activity was assayed by a chemiluminescence method, using a cypridina luciferin analog as a chemiluminescence probe, after extraction from peripheral human polymorphonuclear leukocytes. The chemiluminescence method was based on the detection of 1O2 generated by myeloperoxidase-catalyzed HOBr formation followed by the interaction of HOBr with H2O2 at pH 4.5. ... More
1,2-Diacylglycerol hydroperoxides induce the generation and release of superoxide anion from human polymorphonuclear leukocytes.
AuthorsYamamoto Y, Kambayashi Y, Ito T, Watanabe K, Nakano M
JournalFEBS Lett
PubMed ID9276447
'We examined the effect of 1-palmitoyl-2-linoleoylglycerol (PLG), PLG hydroxide (PLG-OH), and PLG hydroperoxide (PLG-OOH) on the release of superoxide anion from human PMNs monitored by the chemiluminescence generated by the superoxide anion-sensitive reagent, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA). PLG-OOH at low micromolar concentrations stimulated human PMNs whereas PLG and PLG-OH did not. ... More
Detection and cytotoxicity of cisplatin-induced superoxide anion in monolayer cultures of a human ovarian cancer cell line.
AuthorsMasuda H, Tanaka T, Tateishi M, Naito M, Tamai H
JournalCancer Chemother Pharmacol
PubMed ID11269742
'Superoxide anions (O2-) generated by cisplatin [cis-diamminedichloroplatinum (II), DDP] were determined by measuring the chemiluminescence from the luminescence probe, 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (methyl Cypridina luciferin analog, MCLA), in monolayer cultures of a human ovarian cancer cell line (A2780) in physiological saline at pH 7.0. In a time-course study, chemiluminescence of MCLA (C-MCLA) ... More
Hydroxyl radical generation: the effect of bicarbonate, dioxygen and buffer concentration on pH-dependent chemiluminescence.
AuthorsOosthuizen MM, Greyling D
JournalRedox Rep
PubMed ID11450981
'Enhanced chemiluminescence (CL) induced by HO* was detected using the primary enhancers luminol (Lum), isoluminol (ILum) and lucigenin (Luc), in the absence of HCO3-, at pH 9.5, 10.0 and 10.5 but not between pH 4.0 and 9.0. This was confirmed using nine different HO* generators. FeCl3/NTA/H2O2 was the only HO* ... More
Kinetic evaluation of endogenous leukotriene B4 and E4 acute activation of inflammatory cells in the rabbit.
AuthorsCelardo A, Dell'Elba G, Manarini S, Evangelista V, de Gaetano G, Cerletti C
JournalAdv Exp Med Biol
PubMed ID10667365
1,2-Diacylglycerol hydroperoxide induces the generation and release of superoxide anion from human polymorphonuclear leukocytes.
AuthorsYamamoto Y, Kambayashi Y, Ito T, Watanabe K, Nakano M
JournalAdv Exp Med Biol
PubMed ID10667364
Luciferin derivative for assay of myeloperoxidase and dopamine metabolism.
AuthorsNakano M, Koga S
JournalMethods Enzymol
PubMed ID8015485
Radical production and degranulation in pancreatic islets induced by glucose.
AuthorsSakurai T, Terakawa S
JournalJpn J Physiol
PubMed ID9266312
Assay for superoxide dismutase based on chemiluminescence of luciferin analog.
AuthorsNakano M
JournalMethods Enzymol
PubMed ID2233294
Galacturonic-acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo.
AuthorsKim D, Oda T, Ishimatsu A, Muramatsu T
JournalBiosci Biotechnol Biochem
PubMed ID10830520
Red tide phytoplankton, Chattonella marina and Heterosigma akashiwo, are known to generate superoxide anion (O2-). We found that galacturonic acid (GaLUA) stimulated C. marina and H. akashiwo to generate increased amounts of O2-. Since such effect was not observed in any other monosaccharides tested, our results suggest that the binding ... More
Determination of horseradish peroxidase concentration using the chemiluminescence of Cypridina luciferin analogue, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one.
AuthorsMitani M, Yokoyama Y, Ichikawa S, Sawada H, Matsumoto T, Fujimori K, Kosugi M
JournalJ Biolumin Chemilumin
PubMed ID7879651
The chemiluminescence of the Cypridina luciferin analogue, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA) was observed at 462 nm in the presence of horseradish peroxidase (HRP) and the total spectrum of light emitted was found to depend linearly on HRP concentration. Methods for the determination of HRP concentration using the chemiluminescence was investigated. HRP ... More
A high involvement of O2- possibly generated in inner membranes for iron-induced microsomal lipid peroxidation.
AuthorsKoga S, Nakano M
JournalBiochem Biophys Res Commun
PubMed ID1323282
The lipid peroxidation of and the O2- generation by rat liver microsomes in the presence of NADPH or both NADPH and Fe3+ were determined by thiobarbituric acid-reacting substance formation and by chemiluminescence intensities with a cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]pyrazin-3-one(MCLA), as a chemiluminescence probe. Judging from the experiments with various ... More
Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations.
AuthorsTarpey MM, Wink DA, Grisham MB
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID14761864
Facile detection of reactive oxygen and nitrogen species in biologic systems is often problematic. This is a result of the numerous cellular mechanisms, both enzymatic and nonenzymatic involved in their catabolism/decomposition, the complex and overlapping nature of their reactivities, as well as the often limited intracellular access of detector systems. ... More
Inhibitory effects of bromocriptine on phospholipid peroxidation induced by dopa and iron.
AuthorsTanaka M, Sotomatsu A, Yoshida T, Hirai S
JournalNeurosci Lett
PubMed ID7746468
Membrane lipid peroxidation has been suggested to participate in the nigral degeneration of Parkinson's disease. In the present study, we demonstrate that bromocriptine inhibits lipid peroxidation in phospholipid liposomes induced by dopa and iron complexes. Because this lipid peroxidation is not mediated by active oxygen species, antioxidant properties of bromocriptine ... More
Synthetic melanin and ferric ions promote superoxide anion-mediated lipid peroxidation.
AuthorsSotomatsu A, Tanaka M, Hirai S
JournalFEBS Lett
PubMed ID8143859
In this study, we demonstrate that synthetic dopa-melanin produced superoxide anions and promoted the peroxidative cleavage of phospholipids in the presence of Fe(3+)-ADP complexes. SOD significantly suppressed this lipid peroxidation, while catalase or sodium benzoate did not. During the reaction, MCLA-dependent chemiluminescence was detected, and this was suppressed to the ... More
Inhibitory effect of nicergoline on superoxide generation by activated rat microglias measured using a simple chemiluminescence method.
AuthorsYoshida T, Tanaka M, Okamoto K
JournalNeurosci Lett
PubMed ID11114471
We evaluated the effect of nicergoline on superoxide production by rat microglias using a 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]pyrazin-3-one-dependent chemiluminescence assay. Nicergoline dose-dependently inhibited superoxide production by microglias stimulated with phorbol myristate acetate or opsonized zymosan, while it had no effect on superoxide production by a hypoxanthine-xanthine oxidase system. These results indicate that ... More
Maturational changes in superoxide anion production of rabbit alveolar macrophages.
AuthorsKojima T, Hattori K, Fujiwara T, Hirata Y, Kobayashi Y
JournalLife Sci
PubMed ID8057752
We investigated the effect of maturation on the superoxide anion production of rabbit alveolar macrophages by endothelin-1, N-formyl-methionyl-leucyl-phenylalanine, and phorbol 12-myristate 13-acetate. The O2- production by phorbol 12-myristate 13-acetate in young (0.2-0.3 kg) rabbits was as high as that in adult (3.0-3.5 kg) rabbits. However, when alveolar macrophages were stimulated ... More
Mechanism for the generation of superoxide anion and singlet oxygen during heme compound-catalyzed linoleic acid hydroperoxide decomposition.
AuthorsKoga S, Nakano M, Uehara K
JournalArch Biochem Biophys
PubMed ID1654851
Heme compound, hematin or cytochrome c, catalyzes the decomposition of 13-hydroperoxy linoleic acid yielding both O2- and 1O2 under aerobic conditions. No 1O2 is produced when hydrogen peroxide and cumene hydroperoxide are used as substrates. In these experiments, both O2- and 1O2 could be precisely detected by a chemiluminescence method ... More
Inhibitory effects of vasoactive intestinal peptide on superoxide anion formation by N-formyl-methionyl-leucyl-phenylalanine-activated inflammatory cells in vitro.
AuthorsKurosawa M, Ishizuka T
JournalInt Arch Allergy Immunol
PubMed ID8381317
Effects of vasoactive intestinal peptide (VIP) on superoxide anion (O2-) formation by N-formyl-methionyl-leucyl-phenylalanine (fMLP)-activated inflammatory cells from healthy volunteers were investigated using 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo [1,2-a]pyrazin-3-one (MCLA) as a chemiluminescence probe. VIP inhibited the maximal light intensity of MCLA-dependent luminescence in a dose-dependent manner by the activated peripheral blood neutrophils, mononuclear cells ... More
The first observation of O2- generation in in situ lungs of rats treated with drugs to induce experimental acute respiratory distress syndrome.
AuthorsTakahashi A, Nakano M, Mashiko S, Inaba H
JournalFEBS Lett
PubMed ID2155828
To investigate O2- generation in in situ lungs of rats treated with drugs to induce experimental acute respiratory distress syndrome, phorbol myristate acetate (PMA) or endotoxin were injected into rats, who had been continuously infused with 2(methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-alpha]pyraz in-3-one (MCLA) and the chemiluminescence in in situ lung was detected by a ... More
Inhibitory effect of bifemelane on superoxide generation by activated neutrophils measured using a simple chemiluminescence method.
AuthorsYoshida T, Sotomatsu A, Tanaka M, Hirai S
JournalFree Radic Res
PubMed ID7834051
We evaluated the effect of 4-(2-benzylphenoxy)-N-methylbutylamine hydrochloride (bifemelane hydrochloride) on superoxide production by human neutrophils using an MCLA-dependent chemiluminescence assay. Bifemelane hydrochloride dose-dependently inhibited superoxide production by neutrophils stimulated with phorbol myristate acetate, opsonized zymosan, or N-formyl-methionyl-leucyl-phenylalanine, while it had no effect on superoxide production by a hypoxanthine-xanthine oxidase system. ... More
Reevaluation of superoxide scavenging activity of dihydrolipoic acid and its analogues by chemiluminescent method using 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo-[1,2-a]pyrazine-3-one (MCLA) as a superoxide probe.
AuthorsMatsugo S, Konishi T, Matsuo D, Tritschler HJ, Packer L
JournalBiochem Biophys Res Commun
PubMed ID8858128
The superoxide scavenging activity of dihydrolipoic acid and its analogues has been reevaluated by a chemiluminescence method using MCLA as a superoxide indicator. The results demonstrated that all the compounds having the thiol chromophore in the molecule showed scavenging activity toward superoxide. The short-chain analogue, tetranor-dihydrolipoic acid, showed almost the ... More
Involvement of superoxide in acute reaction of angiotensin II in mesenteric microcirculation.
AuthorsKawazoe T, Kosaka H, Yoneyama H, Hata Y
JournalJpn J Physiol
PubMed ID10603428
Superfusion of angiotensin II (Ang II) ceased blood flow in rat mesenteric microcirculation, however, successive reflow occurred. When nitric oxide synthase inhibitor was present, the stoppage of flow occurred by the lower concentration of Ang II. Superoxide dismutase (SOD) significantly delayed the stoppage by Ang II and restored the successive ... More
The antioxidant action of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyra z in-3-one (MCLA), a chemiluminescence probe to detect superoxide anions.
AuthorsTampo Y, Tsukamoto M, Yonaha M
JournalFEBS Lett
PubMed ID9688569
The antioxidant effect of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyraz in-3-one (MCLA), a Cypridina luciferin analog that acts as a chemiluminescence probe to detect O2.-, was investigated. MCLA produced a lag in oxygen consumption induced by cumene hydroperoxide in microsomes or by 2,2'-azobis (2-amidinopropane) dihydrochloride in liposomes and disappeared during the duration of the lag. ... More
Detection of active oxygen species in biological systems.
AuthorsNakano M
JournalCell Mol Neurobiol
PubMed ID9876866
1. Cypridina luciferin analogues, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLD) and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one(CLA ), react with O2- or 1O2 to emit light in visible region. Such chemiluminescences were used for the detection of O2- or 1O2 in activated leukocyte systems and myeloperoxidase (granulocyte-extract) + Br- + H2O2 systems in vitro. 2. The mechanisms of ... More
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
AuthorsWardman P,
JournalFree Radic Biol Med
PubMed ID17761297
Chemical probes for free radicals in biology are important tools; fluorescence and chemiluminescence offer high detection sensitivity. This article reviews progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use. Reactive species include hydrogen peroxide; hydroxyl, superoxide, and thiyl radicals; carbonate ... More
AuthorsBhattacharya A, Muller FL, Liu Y, Sabia M, Liang H, Song W, Jang YC, Ran Q, Van Remmen H,
JournalJ Biol Chem
PubMed ID19001413
Previously, we demonstrated that mitochondria from denervated muscle exhibited dramatically higher Amplex Red dependent fluorescence (thought to be highly specific for hydrogen peroxide) compared with control muscle mitochondria. We now demonstrate that catalase only partially inhibits the Amplex Red signal in mitochondria from denervated muscle. In contrast, ebselen (a glutathione ... More
Detection of superoxide production by activated microglia using a sensitive and specific chemiluminescence assay and microglia-mediated PC12h cell death.
AuthorsTanaka M, Sotomatsu A, Yoshida T, Hirai S, Nishida A
JournalJ Neurochem
PubMed ID8207432
Superoxide production by cultured microglia derived from neonatal rat brains and the cytotoxicity of these cells were evaluated. The chemiluminescence (photon counts) detected in the presence of MCLA, a new chemiluminescence probe, was strongly correlated with the microglial cell count. Chemiluminescence observed in this system was confirmed to originate specifically ... More
Inhibitory effects of azelastine on superoxide anion generation from activated inflammatory cells measured by a simple chemiluminescence method.
AuthorsKurosawa M, Hanawa K, Kobayashi S, Nakano M
JournalArzneimittelforschung
PubMed ID1977394
Blood was drawn from healthy human volunteers and neutrophils and eosinophils were purified on a Conray-Ficoll and a Percoll gradient, respectively. Rat mast cells were also purified on a Percoll gradient. Superoxide anion (O2-) generation from the cells were measured by 2-methyl-6-[p-methoxy-phenyl]-3,7-dihydroimidazo[1,2-a]pyrazin+ ++-3-one (MCLA)-dependent luminescence. Addition of 0.5 mumol/l MCLA ... More
Activated microglia cause superoxide-mediated release of iron from ferritin.
AuthorsYoshida T, Tanaka M, Sotomatsu A, Hirai S
JournalNeurosci Lett
PubMed ID7624046
Ferritin contains the greatest part of the iron found in the brain, and the release of iron stores from ferritin has an essential role in iron-dependent lipid peroxidation. We examined the effect of cultured microglia on iron mobilization from ferritin. Microglia stimulated by phorbol myristate acetate caused the release of ... More
Immunoglobulin G induces microglial superoxide production.
AuthorsYoshida T, Tanaka M, Okamoto K
JournalNeurol Res
PubMed ID12069282
Activating microglias observed in the white matter after chronic cerebral hypoperfusion may play an important role in white matter changes (WMC). Microglial activation has been considered as a result of neuronal damage, however, recently it came to be recognized as a possible cause of the damage in various neurodegenerative diseases. ... More
Oxiradical-dependent photoemission induced by a phacoemulsification probe.
AuthorsShimmura S, Tsubota K, Oguchi Y, Fukumura D, Suematsu M, Tsuchiya M
JournalInvest Ophthalmol Vis Sci
PubMed ID1326494
Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O(2-)-sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7-dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of ... More
Pitfalls of using lucigenin in endothelial cells: implications for NAD(P)H dependent superoxide formation.
AuthorsSohn HY, Keller M, Gloe T, Crause P, Pohl U
JournalFree Radic Res
PubMed ID10730825
Since an increased endothelial superoxide formation plays an important role in the pathogenesis of endothelial dysfunction its specific detection is of particular interest. The widely used superoxide probe lucigenin, however, has been reported to induce superoxide under certain conditions, especially in the presence of NADH. This raises questions as to ... More
The direct effect of interferon-gamma on human eosinophilic leukemia cell lines: the induction of interleukin-5 mRNA and the presence of an interferon-gamma receptor.
AuthorsIshizuka T, Morita K, Hisada T, Ishizuka T, Ando S, Adachi M, Dobashi K, Mori M
JournalInflammation
PubMed ID8728018
The EoL-1 and EoL-3, human eosinophilic leukemia cell lines, have been used as models for studying the maturation and the function of human eosinophils. We investigated the effects of interferon-gamma (IFN-gamma) on superoxide anion (O2-) production of these cell lines and interleukin-5 (IL-5) mRNA expression in the EoL-1. O2- was ... More
Direct measurement of superoxide-dependent chemiluminescence from rat skin following UV-dependent fluoroquinolone-induced dermatitis.
AuthorsWada K, Saniabadi AR, Umemura K, Nakano M, Ito T, Nakashima M
JournalFree Radic Biol Med
PubMed ID7797102
Fluoroquinolones are widely used for the treatment of bacterial infection. However, some members of the group cause UV-dependent dermatitis. Therefore, animal models are required to screen and predict if a derivative is likely to cause photodermatitis. Under anaesthesia, the hair on the dorsal side of rats was shaved, then a ... More
5,5-dimethyl-1-pyrroline-N-oxide alone enhances the spontaneous superoxide generation by primaquine.
AuthorsPrónai L, Ichimori K, Saigusa Y, Nakazawa H
JournalArch Biochem Biophys
PubMed ID1654827
Primaquine (PQ), a well-known antimalarial drug, has been reported to generate superoxide (O2-) in the presence of reducing agents such as NADPH. In the present study, chemiluminescence was detected by adding only PQ to aqueous 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo-[1,2-alpha] pyrazin-3-one (MCLA), which is a specific chemiluminescent probe for O2-, and was quenched by ... More
Effects of azelastine on superoxide anion generation from gamma interferon treated human monoblast cell line U937 measured by chemiluminescence method.
AuthorsKurosawa M, Ishizuka T, Kobayashi S, Nakano M, Yodoi J
JournalArzneimittelforschung
PubMed ID1675571
U937 cells are established human monoblast cell line originally isolated from a patient with diffuse histiocytic lymphoma. In the present study, superoxide anion (O2-) generation from U937 cells were measured by 2-methyl-6-[p-methoxyphenyl]-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLA)-dependent luminescence. Addition of 0.5 mumol/l MCLA and a stimulatory agent, such as N-formyl-methionyl-leucyl- phenylalanine (fMLP) and ... More
Coelenterazine analogs as chemiluminescent probe for superoxide anion.
AuthorsTeranishi K, Shimomura O
JournalAnal Biochem
PubMed ID9193706
Eleven new coelenterazine analogs containing the 3,7-dihydroimidazo[1,2-alpha]pyrazin-3-one structure were synthesized. The superoxide-triggered chemiluminescence of these compounds was investigated using the hypoxanthine-xanthine oxidase system in comparison with four known compounds. The results showed that an alkyl substitution at the position 5 of the imidazopyrazinone ring causes a drastic decrease in the ... More
Chemiluminescence because of the production of reactive oxygen species in the lungs of newborn piglets during resuscitation periods after asphyxiation load.
AuthorsKondo M, Itoh S, Isobe K, Kondo M, Kunikata T, Imai T, Onishi S
JournalPediatr Res
PubMed ID10759161
Reactive oxygen species are regarded as a possible cause of many diseases. However, there are few reports offering in vivo and in situ proof of the direct involvement of reactive oxygen species in the pathogenesis of disease. In the present study, the luciferin derivative 2-methyl-6-[4-methoxyphenyl]-3,7-dihydroimidazo [1,2-alpha] pyrazin-3-one (MCLA) was used ... More
Preventive effect of natural and synthetic antioxidants on lipid peroxidation in the mammalian eye.
AuthorsUeda T, Ueda T, Armstrong D
JournalOphthalmic Res
PubMed ID8829176
Lipid peroxidation was initiated with 5 mM ferric iron and aliquots of bovine or porcine retina, purified rod outer segments (ROS) and retinal pigment epithelium (RPE). After 30 min of oxidation, the lipid hydroperoxide content of the tissues increased approximately 4- to 8-fold over control levels with ROS showing the ... More
Chemiluminescence of 2-methyl-6-arylimidazo-[1,2-a]pyrazin-3(7H)-one in protic solvents: electron-donating substituent effect on the formation of the neutral singlet excited-state molecule.
AuthorsTeranishi K, Hisamatsu M, Yamada T
JournalLuminescence
PubMed ID10602298
2-Methyl-6-arylimidazo[1,2-a]pyrazin-3(7H)-ones with a substituent such as phenyl, 4-methoxyphenyl or 4-trifluoromethoxyphenyl at the 6-position of the imidazo[1,2-a]pyrazin-3(7H)-one ring system, produced chemiluminescence emission in mixtures of water and DMF and in several mixtures of MeOH and DMF under neutral conditions. Under these protic luminescence conditions, the respective light emissions were generated from ... More
Generation of reactive oxygen species by raphidophycean phytoplankton.
AuthorsOda T, Nakamura A, Shikayama M, Kawano I, Ishimatsu A, Muramatsu T
JournalBiosci Biotechnol Biochem
PubMed ID9362113
Chattonella marina, a raphidophycean flagellate, is one of the most toxic red tide phytoplankton and causes severe damage to fish farming. Recent studies demonstrated that Chattonella sp. generates superoxide (O2-), hydrogen peroxide (H2O2), and hydroxyl radicals (.OH), which may be responsible for the toxicity of C. marina. In this study, ... More
Investigation of cyclomaltooligosaccharide-bound 6-(4-methoxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one for enhanced chemiluminescence.
AuthorsTeranishi K, Tanabe S, Hisamatsu M, Yamada T
JournalLuminescence
PubMed ID10602299
The chemiluminescence compound 2-methyl-6-(4-methoxyphenyl)imidazo[1, 2-a]pyrazin-3(7H)-one (MCLA) was attached to cyclomaltooligosaccharides (cyclodextrins) through a single spacer by the formation of an amide bond. The properties of oxygen-induced chemiluminescence of the synthesized cyclodextrin-bound MCLA were investigated in an aqueous phosphate buffer, pH 8.0. The light-emitting efficiency was remarkably dependent on the kind ... More
Superoxide generation by alveolar macrophages from aged rats: improvement by in vitro treatment with IFN-gamma.
AuthorsHayakawa H, Sato A, Yagi T, Uchiyama H, Ide K, Nakano M
JournalMech Ageing Dev
PubMed ID7564571
Alveolar macrophages (AM) from aged rats show an impaired oxidative response, but it is unclear whether or not this is due to the inability of these cells to be activated. To elucidate this, we investigated the capacity of AM from young (16-week-old) and aged (100-week-old) rats to become primed with ... More
Mechanism of superoxide anion generation in the toxic red tide phytoplankton Chattonella marina: possible involvement of NAD(P)H oxidase.
AuthorsKim D, Nakamura A, Okamoto T, Komatsu N, Oda T, Iida T, Ishimatsu A, Muramatsu T
JournalBiochim Biophys Acta
PubMed ID11113571
Red tide phytoplankton Chattonella marina is known to produce reactive oxygen species (ROS), such as superoxide anion (O(2)(-)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (&z.rad;OH), under normal physiological conditions. Although several lines of evidence suggest that ROS are involved in the mortality of fish exposed to C. marina, the mechanism ... More
The ability of neonatal and maternal erythrocytes to produce reactive oxygen species in response to oxidative stress.
AuthorsKondo M, Itoh S, Kusaka T, Imai T, Isobe K, Onishi S
JournalEarly Hum Dev
PubMed ID11872312
The ability of neonatal and maternal erythrocytes to produce reactive oxygen species (ROS) in response to oxidative stress was investigated using the chemiluminescence probe Cypridina luciferin analogue. The chemiluminescence probe, based on 2-methyl-6-[p-methoxyphenyl]-3,7-dihydro-imidazo[1,2-alpha]pyrazin-3-one (MCLA), is highly specific and sensitive to superoxide anion (O(2)(-)) and singlet oxygen (1O(2)). Blood from 11 ... More
Impairment of phagocytic cell respiratory burst by UVA in the presence of fluoroquinolones: an oxygen-dependent phototoxic damage to cell surface microvilli.
AuthorsSaniabadi AR, Wada K, Umemura K, Sakuma S, Nakashima M
JournalJ Photochem Photobiol B
PubMed ID8691354
Fluoroquinolones are widely used clinically as broad-spectrum antimicrobial agents. One of their side effects is UVA-dependent photosensitivity, observed after the skin is exposed to sunlight. We have investigated five fluoroquinolones and have found that their phototoxicity is oxygen dependent. Human phagocytic leucocytes were stimulated with serum opsonized zymosan to produce ... More
Protective effect of nitric oxide against iron-induced neuronal damage.
AuthorsNara K, Konno D, Uchida J, Kiuchi Y, Oguchi K
JournalJ Neural Transm
PubMed ID10599866
We investigated the effect of nitric oxide (NO) on iron-induced neuronal damage. Incubation of PC12 cells after the addition of FeCl2 induced rapid increases (within 1 hr) in lipid peroxidation and a concentration (0.1-2 mM)-dependent decrease in cell viability at 48 hr, both of which were blocked by deferoxamine and ... More
A chemiluminescent detection of superoxide radical produced by adherent leucocytes to the subendothelium following thrombolysis: studies with a photochemically induced thrombosis model in the guinea pig femoral artery.
AuthorsWada K, Umemura K, Nishiyama H, Saniabadi AR, Takiguchi Y, Nakano M, Nakashima M
JournalAtherosclerosis
PubMed ID8769684
Reocclusion following thrombolysis is a major limitation of thrombolytic therapy with recombinant tissue-type plasminogen activator (rt-PA) because denuded vessel wall exposed to blood following thrombolysis is a favourable surface for platelet and leucocyte deposition. We have applied a chemiluminescence technique to detect superoxide radical (0(-2)) produced by leucocytes adherent to ... More
Selectivity and sensitivity in the measurement of reactive oxygen species (ROS) using chemiluminescent microspheres prepared by the binding of acridinium ester or ABEI to polymer microspheres.
AuthorsHosaka S, Itagaki T, Kuramitsu Y
JournalLuminescence
PubMed ID10602307
Two kinds of chemiluminescent microspheres were prepared as tools for measuring reactive oxygen species (ROS) released into phagosomes in phagocytizing cells, by chemically binding acridinium ester or ABEI (isoluminol derivative) to polymer microspheres, and were examined from the viewpoint of specificity and sensitivity to ROS. Acridinium ester-bound microspheres (AE-ms) were ... More
A role for oxidative stress in apoptosis: oxidation and externalization of phosphatidylserine is required for macrophage clearance of cells undergoing Fas-mediated apoptosis.
AuthorsKagan VE, Gleiss B, Tyurina YY, Tyurin VA, Elenström-Magnusson C, Liu SX, Serinkan FB, Arroyo A, Chandra J, Orrenius S, Fadeel B
JournalJ Immunol
PubMed ID12077280
Exposure of phosphatidylserine (PS) on the surface of apoptotic cells has been suggested to serve as an important recognition signal for macrophages. In this work we show that triggering of the death receptor Fas on Jurkat cells results in the generation of reactive oxygen species with oxidation and externalization of ... More
The effect of pH on chemiluminescence of different probes exposed to superoxide and singlet oxygen generators.
AuthorsOosthuizen MM, Engelbrecht ME, Lambrechts H, Greyling D, Levy RD
JournalJ Biolumin Chemilumin
PubMed ID9509335
The compromised optima for high intensity chemiluminescence (CL), using superoxide generators, were all above pH 9.0 for the CL probes luminol and lucigenin. With luminol the optima were at pH 9.0 and 9.4 for the generators KO2 and hypoxanthine/xanthine oxidase (HX/XO), respectively. Lucigenin, with the same generators, produced optima at ... More
Capacitation-associated production of superoxide anion by human spermatozoa.
Authorsde Lamirande E, Gagnon C
JournalFree Radic Biol Med
PubMed ID9101239
Previous results demonstrating the role of the superoxide anion in human sperm capacitation indirectly suggest that the superoxide anion is not only essential to this process, but is also generated by spermatozoa themselves. Our aim was to provide direct evidence for production of superoxide anion by capacitating spermatozoa. Three chemiluminescence ... More
Prostasomes inhibit the NADPH oxidase activity of human neutrophils.
AuthorsSaez F, Motta C, Boucher D, Grizard G
JournalMol Hum Reprod
PubMed ID11006316
Prostasomes are particular lipid vesicles secreted by the prostate in human semen and involved in several physiological functions such as the improvement of sperm motility or immunomodulation. We have previously shown that they reduced the overall reactive oxygen species (ROS) production of seminal polymorphonuclear neutrophils (PMN). The present study was ... More
Chemiluminescence of superoxide generated by Candida albicans: differential effects of the superoxide generator paraquat on a wild-type strain and a respiratory mutant.
AuthorsAoki S, Ito-Kuwa S, Nakamura K, Nakamura Y, Vidotto V, Takeo K
JournalMed Mycol
PubMed ID11860009
We previously reported that a respiration-competent parent strain (K) of Candida albicans was more susceptible to the intracellular superoxide radical (O2-) generator paraquat (PQ) than was a respiration-deficient mutant (KRD-19), although both showed a similar sensitivity to extracellularly generated O2-. To clarify the cause of the differential PQ lethality, we ... More
Involvement of reactive oxygen species in human sperm arcosome reaction induced by A23187, lysophosphatidylcholine, and biological fluid ultrafiltrates.
Authorsde Lamirande E, Tsai C, Harakat A, Gagnon C
JournalJ Androl
PubMed ID9796619
Although recent evidence indicated that the production of reactive oxygen species (ROS) by human spermatozoa may be involved in the regulation of capacitation, very little is known about the role of ROS in the acrosome reaction. To address this issue, Percoll-washed spermatozoa were incubated in Ham's F-10 medium in the ... More
Acceleration of oxidative stress-induced endothelial cell death by nitric oxide synthase dysfunction accompanied with decrease in tetrahydrobiopterin content.
AuthorsIshii M, Shimizu S, Yamamoto T, Momose K, Kuroiwa Y
JournalLife Sci
PubMed ID9252248
The purpose of this study was to examine whether nitric oxide (NO) synthase dysfunction accompanied with decrease in tetrahydrobiopterin (BH4) content increases H2O2-induced endothelial cell death. Endothelial cell death was measured by the release of intracellular lactate dehydrogenase (LDH). Intracellular BH4 content was changed by pretreatment with 2,4-diamino-6-hydroxypyrimidine (DAHP), an ... More
Sinusoidal endothelial cell injury by superoxide anion and iron in the Propionibacterium acnes-pretreated and lipopolysaccharide-stimulated rat liver.
AuthorsHasegawa T, Nakano M, Hashimoto T, Hiraishi K, Suzuki K, Kuwano H
JournalLiver
PubMed ID11903887
AIMS/BACKGROUND: We attempted to measure the generation of superoxide anion, examine its site of release and determine its pathological role in Propionibacterium acnes-lipopolysaccharide-induced liver injury in the rat. METHODS: The P. acnes-pretreated (16 mg/kg i.v.) rat liver was perfused with buffer containing lipopolysaccharide (2.5 microg/ml). Chemiluminescence enhanced with Cypridina luciferin ... More
Propofol prevents endothelial dysfunction induced by glucose overload.
AuthorsKarashima Y, Oike M, Takahashi S, Ito Y
JournalBr J Pharmacol
PubMed ID12381682
Surgical operations often induce acute hyperglycemia, which is known to affect endothelial functions. In this study, we examined the effects of propofol, a commonly used general anaesthetic, on bovine aortic endothelial cell (BAEC) dysfunction induced by glucose overload. 2 D-glucose overload (23 mM) induced an accumulation of superoxide anion (O2-), ... More
Mechanism of superoxide anion production by hepatic sinusoidal endothelial cells and Kupffer cells during short-term ethanol perfusion in the rat.
AuthorsHasegawa T, Kikuyama M, Sakurai K, Kambayashi Y, Adachi M, Saniabadi AR, Kuwano H, Nakano M
JournalLiver
PubMed ID12296966
BACKGROUND/AIMS: The aim of this study was to clarify the candidate cells for and the mechanism of superoxide anion (O2*-) release into the hepatic sinusoids during short-term exposure to ethanol. METHODS: The rat liver was perfused continuously with ethanol (a substrate for alcohol dehydrogenase) or tert-buthanol (not a substrate for ... More
Functional equivalence of Escherichia coli sigma E and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa.
AuthorsYu H, Schurr MJ, Deretic V
JournalJ Bacteriol
PubMed ID7768826
Mucoid colony morphology is the result of the overproduction of the exopolysaccharide alginate and is considered to be a major pathogenic determinant expressed by Pseudomonas aeruginosa during chronic respiratory infections in cystic fibrosis. Conversion to mucoidy can be caused by mutations in the second or third gene of the stress-responsive ... More
A HMG-CoA reductase inhibitor possesses a potent anti-atherosclerotic effect other than serum lipid lowering effects--the relevance of endothelial nitric oxide synthase and superoxide anion scavenging action.
AuthorsSumi D, Hayashi T, Thakur NK, Jayachandran M, Asai Y, Kano H, Matsui H, Iguchi A
JournalAtherosclerosis
PubMed ID11254905
We have determined whether the anti-atherosclerotic effect of a 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibitor (fluvastatin) is mediated through nitric oxide (NO) as well as affecting plasma lipids. NO related vascular responses, endothelial nitric oxide synthase (eNOS) mRNA and superoxide anion (O(2)(-)) release were examined in vascular walls of oophorectomized female rabbits ... More