I have two primary antibodies from the same host species that I wish to use to label the same cells. How do I choose secondary antibodies?
If the two primary antibodies are from different isotypes (such as mouse IgG1 and IgG2a), there are isotype-specific secondary antibodies to choose from. The other option is to make direct conjugates of the primary antibodies through the use of antibody label kits, reactive dyes, or custom conjugations.