Selective proteome-wide detection of hydrophobic integral membrane proteins using a novel fluorescence-based staining technology.
AuthorsHart C, Schulenberg B, Patton WF
JournalElectrophoresis
PubMed ID15300766
Integral proteins containing two or more alpha-helical membrane-spanning domains are underrepresented in two-dimensional gels. While sodium dodecyl sulfate (SDS)-polyacrylamide gels separate these proteins, staining profiles are usually dominated by high-abundance hydrophilic proteins in the specimen. A fluorescence-based stain is presented that selectively highlights integral proteins containing two or more alpha-helical ... More