Binding of monoclonal antibody AA4 to gangliosides on rat basophilic leukemia cells produces changes similar to those seen with Fc epsilon receptor activation.
AuthorsOliver C, Sahara N, Kitani S, Robbins AR, Mertz LM, Siraganian RP
JournalJ Cell Biol
PubMed ID1370498
'The mAb AA4 binds to novel derivatives of the ganglioside Gd1b on rat basophilic leukemia (RBL-2H3) cells. Some of the gangliosides are located close to the high affinity IgE receptor (Fc epsilon RI), and binding of mAb AA4 inhibits Fc epsilon RI-mediated histamine release. In the present study, mAb AA4 ... More
Fluorescent tetradecanoylphorbol acetate: a novel probe of phorbol ester binding domains.
AuthorsBalázs M, Szöllösi J, Lee WC, Haugland RP, Guzikowski AP, Fulwyler MJ, Damjanovich S, Feuerstein BG, Pershadsingh HA
JournalJ Cell Biochem
PubMed ID1663503
Protein kinase C (PKC) has a prominent role in signal transduction of many bioactive substances. We synthesized the fluorescent derivative, phorbol-13-acetate-12-N-methyl-N-4-(N,N'-di(2-hydroxyethyl)amino)-7-n itr obenz-2-oxa-1,3-diazole-aminododecanoate (N-C12-Ac(13)) of 12-O-tetradecanoylphorbol-13-acetate (TPA) to monitor the location of phorbol ester binding sites and evaluate its potential use as a probe of PKC in viable cells. The ... More