MitoProbe™ 转换孔检测试剂盒，用于流式细胞分析

引用和文献 (16)

Invitrogen™

MitoProbe™ 转换孔检测试剂盒，用于流式细胞分析

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability了解更多信息

货号	数量
M34153	1 kit

货号 M34153

价格（CNY)

6,928.00

Ends: 31-Dec-2025

9,205.00

共减 2,277.00 (25%)

添加至购物车

1 kit

价格（CNY)

6,928.00

Ends: 31-Dec-2025

9,205.00

共减 2,277.00 (25%)

添加至购物车

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.

仅供科研使用。不可用于诊断程序。

激发/发射488

反应次数100 Reactions

产品线MitoProbe

产品类型转换孔检测试剂盒

数量1 kit

运输条件室温

偶联物钙黄绿素 AM

产品规格管装

Unit SizeEach

内容与储存

包含 5 样品瓶钙黄绿素，AM（50 µg 干粉/瓶）、1 样品瓶钴 (II) 氯六水合物（1.2 mL，80 mM 的盐水溶液）、1 样品瓶离子霉素 (55 µg)、钙盐和 1 样品瓶 DMSO (1.5 mL)。

在冷冻冰箱（-5 至 -30°C）中储存。

常见问题解答 (FAQ)

流式细胞术的优势有哪些？

•可测定来自单个细胞的数据。
•可从大量细胞中获得数据，产生细胞群的丰富统计学分析结果。
•由于可测定单个细胞，能够揭示种群的异质性。
•支持多重检测，可鉴定小型亚群。
•可以快速分析数以千计的细胞。
•非常适合血液样本和其他悬浮细胞。
•数据获取后，可以多次重复分析。
•流式细胞仪文件（FCS）可以归档。

我能在流式细胞仪上进行哪些应用？

可进行多种应用，包括免疫分型、细胞周期分析、凋亡检测（如膜联蛋白V染色检测实验）、CellEvent Caspase-3/7检测、TUNEL检测、细胞活性检测、增殖检测（如CellTrace 检测和Click-iT EdU检测）、MitoProbe检测法测定线粒体电势、利用计数微球进行细胞计数。

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

引用和文献 (16)

引用和文献

Abstract

Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore.

Authors:Nguyen TT, Stevens MV, Kohr M, Steenbergen C, Sack MN, Murphy E,

Journal:J Biol Chem

PubMed ID:21930693

The mitochondrial permeability transition pore (mPTP) opening plays a critical role in mediating cell death during ischemia/reperfusion (I/R) injury. Our previous studies have shown that cysteine 203 of cyclophilin D (CypD), a critical mPTP mediator, undergoes protein S-nitrosylation (SNO). To investigate the role of cysteine 203 in mPTP activation, we ... More

Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.

Authors:Petronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F

Journal:Biophys J

PubMed ID:9929477

'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More

Calpain and caspase orchestrated death signal to accomplish apoptosis induced by resveratrol and its novel analog hydroxystilbene-1 [correction of hydroxstilbene-1] in cancer cells.

Authors:Guha P, Dey A, Dhyani MV, Sen R, Chatterjee M, Chattopadhyay S, Bandyopadhyay SK,

Journal:J Pharmacol Exp Ther

PubMed ID:20484155

'Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol is a promising chemopreventive compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid of such an ulcerogenic side effect. Consequently, here we tried to explore the chemopreventive efficacy ... More

Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts.

Authors:Cotán D, Cordero MD, Garrido-Maraver J, Oropesa-Ávila M, Rodríguez-Hernández A, Gómez Izquierdo L, De la Mata M, De Miguel M, Lorite JB, Infante ER, Jackson S, Navas P, Sánchez-Alcázar JA,

Journal:FASEB J

PubMed ID:21551238

Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease most usually caused by point mutations in tRNA genes encoded by mtDNA. Here, we report on how this mutation affects mitochondrial function in primary fibroblast cultures established from 2 patients with MELAS who harbored the A3243G mutation. Both ... More

Imaging the mitochondrial permeability transition pore in intact cells.

Authors:Petronilli V, Miotto G, Canton M, Colonna R, Bernardi P, Di Lisa F

Journal:Biofactors

PubMed ID:9914828

The involvement of mitochondrial permeability transition pore (MTP) in cellular processes is generally investigated by indirect means, such as changes in mitochondrial membrane potential or pharmacological inhibition. However, such effects could not be related univocally to MTP. In addition, source of errors could be represented by the increased retention of ... More

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