MitoSOX™ Green Mitochondrial Superoxide Indicator, for live-cell imaging, 1 vial x 9 μg - FAQs

View additional product information for MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging - FAQs (M36009, M36008, M36005, M36007, M36006)

6 product FAQs found

Are MitoSOX Mitochondrial Superoxide Indicators fixable?

MitoSOX indicator-stained cells should be imaged within 2 hr and are not fixable.

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What is the shipping temperature for MitoSOX Mitochondrial Superoxide Indicators, for live-cell imaging?

MitoSOX Mitochondrial Superoxide Indicators, for live-cell imaging are shipped at ambient temperature and should be stored as recommended.

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Can I use MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) with other ROS reagents that don't accumulate within the mitochondria?

We do not recommend using MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) with other ROS reagents. Other ROS reagents may compete for radicals in or around the mitochondria limiting the response of the MitoSOX reagents.

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Can I store stock solutions of MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) beyond one day?

No. We do not recommend storing MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) stock solutions beyond one day as they are more susceptible to spontaneous oxidation (from radicals in the atmosphere) when in solution compared to storing the reagents in solid form.

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Does MitoSOX Green Mitochondrial Superoxide Indicator (Cat. Nos. M36005, M36006) bind to nucleic acids after oxidation?

Upon oxidation, MitoSOX Green Mitochondrial Superoxide Indicator (Cat. Nos. M36005, M36006) does not bind to nucleic acids and unlike MitoSOX Red Mitochondrial Superoxide Indicator (Cat. Nos. M36007, M36008), does not require binding to nucleic acids to enhance its fluorescence.

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Is MitoSOX Green Mitochondrial Superoxide Indicator (Cat. No. M36005, M36006) less responsive to other radicals (not superoxide) compared to MitoSOX Red Mitochondrial Superoxide Indicator (Cat. No. M36007, M36008)?

According to Figure 1 of the MitoSOX Green and MitoSOX Red Mitochondrial Superoxide Indicators User Guide, MitoSOX Red shows a slightly higher propensity to oxidation by other radicals compared to MitoSOX Green.

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