MitoSOX™ 线粒体超氧化物指示剂,用于活细胞成像
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MitoSOX™ 线粒体超氧化物指示剂,用于活细胞成像
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MitoSOX™ 线粒体超氧化物指示剂,用于活细胞成像

MitoSOX 超氧化物指示剂是一种新型荧光染料,专门靶向活细胞中的线粒体。利用线粒体超氧化物氧化 MitoSOX 试剂产生明亮的绿色或红色荧光。
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货号数量激发/发射指示器颜色
M360071 小瓶∼396/610 nmMitoSox Red红色
M360051 小瓶∼488/510 nmMitoSox Green绿色
M360065 小瓶∼488/510 nmMitoSox Green绿色
M3600810 小瓶∼396/610 nmMitoSox Red红色
M360092样品瓶(1样品瓶为绿色、1样品瓶为红色)488/510 nm(绿色),396/610 nm(红色)MitoSox Green, MitoSox Red绿色、红色
货号 M36007
价格(CNY)
1,448.00
飞享价
Ends: 31-Dec-2025
1,951.00
共减 503.00 (26%)
Each
添加至购物车
数量:
1 小瓶
激发/发射:
∼396/610 nm
指示器:
MitoSox Red
颜色:
红色
价格(CNY)
1,448.00
飞享价
Ends: 31-Dec-2025
1,951.00
共减 503.00 (26%)
Each
添加至购物车
MitoSOX Green 和 MitoSOX Red 超氧化物指示剂是一种新型荧光染料,专门靶向活细胞中的线粒体。利用线粒体超氧化物氧化 MitoSOX 试剂产生明亮的绿色或红色荧光。
这些指示剂的特点包括:
•易被超氧化物氧化,但不易被其他 ROS 或 RNS 生成系统氧化
• 用于活细胞成像
• 快速且选择性地靶向线粒体
• MitoSOX Green 指示剂:上限吸收/发射波长 ∼488/510 nm(传统 FITC/GFP 滤光片组)
• MitoSox Red 指示剂:上限吸收/发射波长 ∼396/610 nm*(推荐自定义滤光片组)

*Robinson 等人(1) 报告,如果 MitoSOX Red 染料在 396 nm 而非 510 nm 处激发,它可能是线粒体超氧化物的更具选择性标记物。

MitoSOX Green 指示剂以一样品瓶或一包五样品瓶的形式提供。MitoSOX Red 指示剂以一样品瓶或一包十样品瓶的形式提供。我们还提供 MitoSOX Red 和 MitSOX Green 指示剂各一瓶的一件混合装。

快速、简单地检测活细胞中的线粒体超氧化物
可利用 MitoSOX 超氧化物指示剂通过荧光显微镜检查查看线粒体产生超氧化物。指示剂渗透活细胞并在其中选择性地靶向线粒体。这些试剂被超氧化物迅速氧化,但不被其他活性氧 (ROS) 和活性氮 (RNS) 氧化。氧化产物发出强烈的荧光。

MitoSOX 指示剂可用于辨别分离线粒体制备物的伪影与活细胞线粒体中生成的超氧化物的直接测量结果。在研究各种病理中调节氧化应激的因子时,它们也可成为有价值的工具。此外,这些指示剂还用于使用高内涵仪器的代谢通量测定 (2)。MitoSOX 指示剂也用于使用流式细胞分析检测线粒体超氧化物 (3)。

参考文献
:1:Robinson, Kristine M., et al.Selective fluorescent imaging of superoxide in vivo using ethidium-based probes.Proceedings of the National Academy of Sciences 103.41 (2006): 15038-15043.
2: Little, Andrew Charles, et al.High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions. Communications biology 3.1 (2020): 1-10.
3: Kauffman, Megan E. et al.MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS.Reactive oxygen species (Apex, N.C.) vol. 2,5 (2016): 361-370.

仅供科研使用。不可用于诊断程序。
规格
颜色红色
数量1 小瓶
检测方法荧光
激发/发射∼396/610 nm
指示器MitoSox Red
产品线MitoSOX™
Unit SizeEach

常见问题解答 (FAQ)

Are MitoSOX Mitochondrial Superoxide Indicators fixable?

MitoSOX indicator-stained cells should be imaged within 2 hr and are not fixable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the shipping temperature for MitoSOX Mitochondrial Superoxide Indicators, for live-cell imaging?

MitoSOX Mitochondrial Superoxide Indicators, for live-cell imaging are shipped at ambient temperature and should be stored as recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why was the excitation wavelength for MitoSOX Red (Cat. Nos. M36008, M36007, M36009) changed from 510 nm to 396 nm in the new manual? What is the optimal wavelength to measure the signal?

The excitation wavelength for MitoSOX Red (Cat. Nos. M36008, M36007, M36009) was changed from 510 nm to 396 nm in the new manual because we have found that excitation at 396 nm selectively excites the superoxide oxidation product without exciting other non-specific products.  Although the indicator displays an adequate signal when excited at 510 nm, we highly recommend using the 396 nm excitation wavelength for selective detection of the indicator’s response to mitochondrial superoxide. For more information on the selective detection of superoxide at 396 nm, please refer to the article linked below.

Robinson KM, Janes MS, Pehar M, Monette JS, Ross MF, Hagen TM, Murphy MP, Beckman JS. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15038-43. doi: 10.1073/pnas.0601945103. Epub 2006 Oct 2. PMID: 17015830; PMCID: PMC1586181.

The optimal emission wavelength to observe the superoxide oxidation product is 580 nm. Please note that the 610 nm wavelength mentioned in the MitoSOX Green and MitoSOX Red Mitochondrial Superoxide Indicators User Guide is incorrect.

We suggest a working concentration range of 100 nM to 5 µM.  It is recommended to optimize the final working concentration for each sample and application. It is important to be cautious when using concentrations >1 µM, as higher non-specific labeling may occur as well as possible mitochondrial toxicity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) with other ROS reagents that don't accumulate within the mitochondria?

We do not recommend using MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) with other ROS reagents. Other ROS reagents may compete for radicals in or around the mitochondria limiting the response of the MitoSOX reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I store stock solutions of MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) beyond one day?

No. We do not recommend storing MitoSOX Mitochondrial Superoxide Indicators (Cat. Nos. M36005, M3006, M36007, M36008, M36009) stock solutions beyond one day as they are more susceptible to spontaneous oxidation (from radicals in the atmosphere) when in solution compared to storing the reagents in solid form.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.