Substrate translocation kinetics of excitatory amino acid carrier 1 probed with laser-pulse photolysis of a new photolabile precursor of D-aspartic acid.
AuthorsGrewer C, Madani Mobarekeh SA, Watzke N, Rauen T, Schaper K
JournalBiochemistry
PubMed ID11141075
'Here we report the synthesis and photochemical and biological characterization of a new photolabile precursor of D-aspartic acid, alpha-carboxynitrobenzyl-caged D-aspartate (alpha-CNB-caged D-aspartate), and its application for studying the molecular mechanism of the neuronal excitatory amino acid carrier 1 (EAAC1). Investigation of the photochemical properties of alpha-CNB-caged D-aspartate by transient absorption ... More
Compartmentalized and binary behavior of terminal dendrites in hippocampal pyramidal neurons.
AuthorsWei DS, Mei YA, Bagal A, Kao JP, Thompson SM, Tang CM
JournalScience
PubMed ID11567143
'The dendritic arbor of pyramidal neurons is not a monolithic structure. We show here that the excitability of terminal apical dendrites differs from that of the apical trunk. In response to fluorescence-guided focal photolysis of caged glutamate, individual terminal apical dendrites generated cadmium-sensitive all-or-none responses that were subthreshold for somatic ... More