Bcl-2 controls caspase activation following a p53-dependent cyclin D1-induced death signal.
AuthorsPratt MA, Niu MY
JournalJ Biol Chem
PubMed ID12480939
'MCF-7 and ZR-75 breast cancer cells infected with an adenovirus constitutively expressing high levels of cyclin D1 demonstrated widespread mitochondrial translocation of Bax and cytochrome c release that was approximately doubled after the addition of all-trans retinoic acid (RA) or Bcl-2 antisense oligonucleotide. By comparison, the percentage of cells in ... More
Ketamine induces toxicity in human neurons differentiated from embryonic stem cells via mitochondrial apoptosis pathway.
AuthorsBosnjak ZJ, Yan Y, Canfield S, Muravyeva MY, Kikuchi C, Wells CW, Corbett JA, Bai X,
JournalCurr Drug Saf
PubMed ID22873495
'Ketamine is widely used for anesthesia in pediatric patients. Growing evidence indicates that ketamine causes neurotoxicity in a variety of developing animal models. Our understanding of anesthesia neurotoxicity in humans is currently limited by difficulties in obtaining neurons and performing developmental toxicity studies in fetal and pediatric populations. It may ... More
Spatiotemporal compartmentalization of key physiological processes during muscle precursor differentiation.
AuthorsOzbudak EM, Tassy O, Pourquié O,
JournalProc Natl Acad Sci U S A
PubMed ID20160088
'The development of multicellular organisms is controlled by transcriptional networks. Understanding the role of these networks requires a full understanding of transcriptome regulation during embryogenesis. Several microarray studies have characterized the temporal evolution of the transcriptome during development in different organisms [Wang QT, et al. (2004) Dev Cell 6:133-144; Furlong ... More
'Huntington disease (HD) is caused by a pathological elongation of CAG repeats in the huntingtin protein gene and is characterized by atrophy and neuronal loss primarily in the striatum. Mitochondrial dysfunction and impaired Ca2+ homeostasis in HD have been suggested previously. Here, we elucidate the effects of Ca2+ on mitochondria ... More
Motor deficit in a Drosophila model of mucolipidosis type IV due to defective clearance of apoptotic cells.
'Disruption of the Transient Receptor Potential (TRP) mucolipin 1 (TRPML1) channel results in the neurodegenerative disorder mucolipidosis type IV (MLIV), a lysosomal storage disease with severe motor impairments. The mechanisms underlying MLIV are poorly understood and there is no treatment. Here, we report a Drosophila MLIV model, which recapitulates the ... More
AAT-1, a novel testis-specific AMY-1-binding protein, forms a quaternary complex with AMY-1, A-kinase anchor protein 84, and a regulatory subunit of cAMP-dependent protein kinase and is phosphorylated by its kinase.
AuthorsYukitake H, Furusawa M, Taira T, Iguchi-Ariga SM, Ariga H
JournalJ Biol Chem
PubMed ID12223483
'AMY-1 has been identified by us as a c-Myc-binding protein and was found to stimulate c-Myc transcription activity. AMY-1 was also found to be associated with protein kinase A anchor protein 84/149 (S-AKAP84/AKAP149) in the mitochondria in somatic cells and sperm, suggesting that it plays a role in spermatogenesis. To ... More
An incremental approach to automated protein localisation.
AuthorsTscherepanow M, Jensen N, Kummert F,
JournalBMC Bioinformatics
PubMed ID18937856
'BACKGROUND: The subcellular localisation of proteins in intact living cells is an important means for gaining information about protein functions. Even dynamic processes can be captured, which can barely be predicted based on amino acid sequences. Besides increasing our knowledge about intracellular processes, this information facilitates the development of innovative ... More
Activation of serine/threonine protein phosphatase-1 is required for ceramide-induced survival of sympathetic neurons.
AuthorsPlummer G, Perreault KR, Holmes CF, Posse De Chaves EI
JournalBiochem J
PubMed ID15361069
'In sympathetic neurons, C6-ceramide, as well as endogenous ceramides, blocks apoptosis elicited by NGF (nerve growth factor) deprivation. The mechanism(s) involved in ceramide-induced neuronal survival are poorly understood. Few direct targets for the diverse cellular effects of ceramide have been identified. Amongst those proposed is PP-1c, the catalytic subunit of ... More
Direct evidence of nitric oxide presence within mitochondria.
'Nitric oxide (NO) has been implicated in the modulation of mitochondrial respiration, membrane potential, and subsequently in apoptosis. Although the presence of a mitochondrial NO synthase (mtNOS) has been described, there is no direct evidence in vivo of the presence of NO within mitochondria. It was the aim of this ... More
AuthorsJenei A, Kirsch AK, Subramaniam V, Arndt-Jovin DJ, Jovin TM
JournalBiophys J
PubMed ID9916041
'We have implemented simultaneous picosecond pulsed two- and three-photon excitation of near-UV and visible absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 1064-nm emission from a pulsed Nd:YVO4 laser was used to excite the visible mitochondrial specific dye MitoTracker Orange CM-H2TMRos or a Cy3-labeled antibody by two-photon excitation, ... More
ZnT-2, a mammalian protein that confers resistance to zinc by facilitating vesicular sequestration.
AuthorsPalmiter RD, Cole TB, Findley SD
JournalEMBO J
PubMed ID8617223
'A cDNA encoding a second zinc transporter (ZnT-2) was isolated from a rat kidney cDNA expression library by complementation of a zinc-sensitive BHK cell line. The protein predicted from the open reading frame of ZnT-2 cDNA has 359 amino acids and initiates with a CTG codon. It resembles ZnT-1 (a ... More
Controlling the intracellular localization of fluorescent polyamide analogues in cultured cells.
AuthorsCrowley KS, Phillion DP, Woodard SS, Schweitzer BA, Singh M, Shabany H, Burnette B, Hippenmeyer P, Heitmeier M, Bashkin JK
JournalBioorg Med Chem Lett
PubMed ID12699756
The intracellular distribution of fluorescent-labeled polyamides was examined in live cells. We showed that BODIPY-labeled polyamides accumulate in acidic vesicles, mainly lysosomes, in the cytoplasm of HCT116 colon cancer cells and human rheumatoid synovial fibroblasts (RSF). Verapamil blocked vesicular accumulation and led to nuclear accumulation of the BODIPY-labeled polyamide in ... More
Real-time measurement of intracellular reactive oxygen species using Mito tracker orange (CMH2TMRos).
AuthorsKweon SM, Kim HJ, Lee ZW, Kim SJ, Kim SI, Paik SG, Ha KS
JournalBiosci Rep
PubMed ID11893000
We have investigated a novel method to monitor real changes of intracellular ROS by the use of CMH2TMRos (a reduced form of MitoTracker orange) in Swiss 3T3 fibroblasts. Arachidonic acid induced a rapid increase of CMTMRos fluorescence with a maximal elevation at 120-150 sec, which was determined by scanning every ... More
Bcl-2 inhibits a Fas-induced conformational change in the Bax N terminus and Bax mitochondrial translocation.
AuthorsMurphy KM, Streips UN, Lock RB
JournalJ Biol Chem
PubMed ID10751382
Members of the Bcl-2 family of proteins control the cellular commitment to apoptosis, although their role in Fas-induced apoptosis is ill-defined. In this report we demonstrate that activation of the Fas receptor present on a human breast epithelial cell line resulted in a conformational change in the N terminus of ... More
Measurement of mitochondrial pH in situ.
AuthorsTakahashi A, Zhang Y, Centonze E, Herman B
JournalBiotechniques
PubMed ID11314264
In this article, we describe the advantages and disadvantages of procedures for monitoring mitochondrial pH in situ using optical microscopic techniques. The first method employs the combination of the fluorescent pH-sensitive indicator carboxy-SNARF and laser scanning confocal microscopy. Manipulation of the loading and post-loading conditions enables relatively specific accumulation of ... More
The cellular target of histatin 5 on Candida albicans is the energized mitochondrion.
AuthorsHelmerhorst EJ, Breeuwer P, van't Hof W, Walgreen-Weterings E, Oomen LC, Veerman EC, Amerongen AV, Abee T
JournalJ Biol Chem
PubMed ID10066791
Histatin 5 is a human basic salivary peptide with strong fungicidal properties in vitro. To elucidate the mechanism of action, the effect of histatin 5 on the viability of Candida albicans cells was studied in relation to its membrane perturbing properties. It was found that both the killing activity and ... More
Mitochondrial targeting and a novel transmembrane arrest of Alzheimer's amyloid precursor protein impairs mitochondrial function in neuronal cells.
AuthorsAnandatheerthavarada HK, Biswas G, Robin MA, Avadhani NG
JournalJ Cell Biol
PubMed ID12695498
Alzheimer's amyloid precursor protein 695 (APP) is a plasma membrane protein, which is known to be the source of the toxic amyloid beta (Abeta) peptide associated with the pathogenesis of Alzheimer's disease (AD). Here we demonstrate that by virtue of its chimeric NH2-terminal signal, APP is also targeted to mitochondria ... More
Chondrocyte protein with a poly-proline region (CHPPR) is a novel mitochondrial protein and promotes mitochondrial fission.
AuthorsTonachini L, Monticone M, Puri C, Tacchetti C, Pinton P, Rizzuto R, Cancedda R, Tavella S, Castagnola P
JournalJ Cell Physiol
PubMed ID15389597
We have recently identified a chondrocyte protein with a poly-proline region, referred to as CHPPR, and showed that this protein is expressed intracellularly in chick embryo chondrocytes. Conventional fluorescence and confocal localization of CHPPR shows that CHPPR is sorted to mitochondria. Furthermore, immunoelectron microscopy of CHPPR transfected cells demonstrates that ... More
Gaf-1, a gamma -SNAP-binding protein associated with the mitochondria.
AuthorsChen D, Xu W, He P, Medrano EE, Whiteheart SW
JournalJ Biol Chem
PubMed ID11278501
The role of alpha/beta-SNAP (Soluble NSF Attachment Protein) in vesicular trafficking is well established; however, the function of the ubiquitously expressed gamma-SNAP remains unclear. To further characterize the cellular role of this enigmatic protein, a two-hybrid screen was used to identify new, gamma-SNAP-binding proteins and to uncover potentially novel functions ... More
Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase.
AuthorsGuo K, Lukacik P, Papagrigoriou E, Meier M, Lee WH, Adamski J, Oppermann U
JournalJ Biol Chem
PubMed ID16380372
Human DHRS6 is a previously uncharacterized member of the short chain dehydrogenases/reductase family and displays significant homologies to bacterial hydroxybutyrate dehydrogenases. Substrate screening reveals sole NAD(+)-dependent conversion of (R)-hydroxybutyrate to acetoacetate with K(m) values of about 10 mm, consistent with plasma levels of circulating ketone bodies in situations of starvation ... More
Bradykinin induces mitochondrial ROS generation via NO, cGMP, PKG, and mitoKATP channel opening and leads to cardioprotection.
AuthorsOldenburg O, Qin Q, Krieg T, Yang XM, Philipp S, Critz SD, Cohen MV, Downey JM
JournalAm J Physiol Heart Circ Physiol
PubMed ID12958031
Bradykinin (BK) mimics ischemic preconditioning by generating reactive oxygen species (ROS). To identify intermediate steps that lead to ROS generation, rabbit cardiomyocytes were incubated in reduced MitoTracker Red stain, which becomes fluorescent after exposure to ROS. Fluorescence intensity in treated cells was expressed as a percentage of that in paired, ... More
Identification of a peroxisomal acyl-activating enzyme involved in the biosynthesis of jasmonic acid in Arabidopsis.
AuthorsKoo AJ, Chung HS, Kobayashi Y, Howe GA
JournalJ Biol Chem
PubMed ID16963437
Jasmonic acid (JA) is a lipid-derived signal that regulates a wide variety of developmental and defense-related processes in higher plants. JA is synthesized from linolenic acid via an enzymatic pathway that initiates in the plastid and terminates in peroxisomes. The C18 JA precursor 12-oxo-phytodienoic acid (OPDA) is converted in the ... More
Mitochondrial effects with ceramide-induced cardiac apoptosis are different from those of palmitate.
AuthorsKong JY, Rabkin SW
JournalArch Biochem Biophys
PubMed ID12667483
The objective of this study was to evaluate whether ceramide, palmitate, and inhibitors of mitochondrial electron transport chain shared similar effects on the mitochondria of intact cardiomyocytes in order to determine the likelihood that ceramide and palmitate utilize similar mitochondrial mechanisms or pathways to apoptosis. In embryonic chick cardiomyocytes, ceramide, ... More
Mitotic Motor KIFC1 Is an Organizer of Microtubules in the Axon.
Authors
JournalJ Neurosci
PubMed ID30804089
Rhes travels from cell to cell and transports Huntington disease protein via TNT-like protrusion.
Authors
JournalJ Cell Biol
PubMed ID31076452
Methylated HNRNPK acts on RPS19 to regulate ALOX15 synthesis in erythropoiesis.
Authors
JournalNucleic Acids Res
PubMed ID33660773
Essential role of the redox-sensitive kinase p66shc in determining energetic and oxidative status and cell fate in neuronal preconditioning.
Authors
JournalJ Neurosci
PubMed ID20392947
The Arabidopsis thiamin-deficient mutant pale green1 lacks thiamin monophosphate phosphatase of the vitamin B1 biosynthesis pathway.