Mitochondrial transcription factor A induction by redox activation of nuclear respiratory factor 1.
AuthorsPiantadosi CA,Suliman HB
JournalThe Journal of biological chemistry
PubMed ID16230352
Authors
Journal
PubMed ID18258751
A retinoic acid--rich tumor microenvironment provides clonal survival cues for tumor-specific CD8(+) T cells.
AuthorsGuo Y, Pino-Lagos K, Ahonen CA, Bennett KA, Wang J, Napoli JL, Blomhoff R, Sockanathan S, Chandraratna RA, Dmitrovsky E, Turk MJ, Noelle RJ,
JournalCancer Res
PubMed ID22902413
While vitamin A has been implicated in host resistance to infectious disease, little is known about the role of vitamin A and its active metabolite, retinoic acid (RA) in host defenses against cancer. Here, we show that local RA production within the tumor microenvironment (TME) is increased up to 5-fold ... More
A hyperfused mitochondrial state achieved at G1-S regulates cyclin E buildup and entry into S phase.
AuthorsMitra K, Wunder C, Roysam B, Lin G, Lippincott-Schwartz J,
JournalProc Natl Acad Sci U S A
PubMed ID19617534
Mitochondria undergo fission-fusion events that render these organelles highly dynamic in cells. We report a relationship between mitochondrial form and cell cycle control at the G(1)-S boundary. Mitochondria convert from isolated, fragmented elements into a hyperfused, giant network at G(1)-S transition. The network is electrically continuous and has greater ATP ... More
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
AuthorsCoatesworth W, Bolsover S
JournalCell Calcium
PubMed ID16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into ... More
An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle.
AuthorsShima DT, Cabrera-Poch N, Pepperkok R, Warren G
JournalJ Cell Biol
PubMed ID9585414
'During mitosis, the ribbon of the Golgi apparatus is transformed into dispersed tubulo-vesicular membranes, proposed to facilitate stochastic inheritance of this low copy number organelle at cytokinesis. Here, we have analyzed the mitotic disassembly of the Golgi apparatus in living cells and provide evidence that inheritance is accomplished through an ... More
Translocation of SAPK/JNK to mitochondria and interaction with Bcl-x(L) in response to DNA damage.
AuthorsKharbanda S, Saxena S, Yoshida K, Pandey P, Kaneki M, Wang Q, Cheng K, Chen YN, Campbell A, Sudha T, Yuan ZM, Narula J, Weichselbaum R, Nalin C, Kufe D
JournalJ Biol Chem
PubMed ID10617621
'Activation of the stress-activated protein kinase (SAPK/JNK) by genotoxic agents is necessary for induction of apoptosis. We report here that ionizing radiation ionizing radiation exposure induces translocation of SAPK to mitochondria and association of SAPK with the anti-apoptotic Bcl-x(L) protein. SAPK phosphorylates Bcl-x(L) on threonine 47 (Thr-47) and threonine 115 ... More
Targeting of the c-Abl tyrosine kinase to mitochondria in the necrotic cell death response to oxidative stress.
AuthorsKumar S, Bharti A, Mishra NC, Raina D, Kharbanda S, Saxena S, Kufe D
JournalJ Biol Chem
PubMed ID11350980
'The ubiquitously expressed c-Abl tyrosine kinase is activated in the response of cells to genotoxic and oxidative stress. The present study demonstrates that reactive oxygen species (ROS) induce targeting of c-Abl to mitochondria. We show that ROS-induced localization of c-Abl to mitochondria is dependent on activation of protein kinase C ... More
Inhibition of wild-type p66ShcA in mesangial cells prevents glycooxidant-dependent FOXO3a regulation and promotes the survival phenotype.
AuthorsChintapalli J, Yang S, Opawumi D, Goyal SR, Shamsuddin N, Malhotra A, Reiss K, Meggs LG,
JournalAm J Physiol Renal Physiol
PubMed ID17077388
'Hyperglycemia triggers an exponential increase in reactive oxygen species (ROS) at the cellular level. Here, we demonstrate induction of the oxidant-resistant phenotype in mesangial cells by silencing the wild-type (WT) p66ShcA gene. Two approaches were employed to inhibit WTp66ShcA in SV40 murine mesangial cells and normal human mesangial cells: transient ... More
Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.
AuthorsDagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
JournalJ Biol Chem
PubMed ID19279012
'Mitochondrial dysregulation is strongly implicated in Parkinson disease. Mutations in PTEN-induced kinase 1 (PINK1) are associated with familial parkinsonism and neuropsychiatric disorders. Although overexpressed PINK1 is neuroprotective, less is known about neuronal responses to loss of PINK1 function. We found that stable knockdown of PINK1 induced mitochondrial fragmentation and autophagy ... More
An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation.
AuthorsSharp MD, Pogliano K
JournalProc Natl Acad Sci U S A
PubMed ID10588743
'Shortly after the synthesis of the two cells required for sporulation in Bacillus subtilis, the membranes of the larger mother cell begin to migrate around and engulf the smaller forespore cell. At the completion of this process the leading edges of the migrating membrane meet and fuse, releasing the forespore ... More
SM-20 is a novel mitochondrial protein that causes caspase-dependent cell death in nerve growth factor-dependent neurons.
AuthorsLipscomb EA, Sarmiere PD, Freeman RS
JournalJ Biol Chem
PubMed ID11060309
'Sympathetic neurons undergo protein synthesis-dependent apoptosis when deprived of nerve growth factor (NGF). Expression of SM-20 is up-regulated in NGF-deprived sympathetic neurons, and ectopic SM-20 is sufficient to promote neuronal death in the presence of NGF. We now report that SM-20 is a mitochondrial protein that promotes cell death through ... More
Mitochondrial association of a plus end-directed microtubule motor expressed during mitosis in Drosophila.
AuthorsPereira AJ, Dalby B, Stewart RJ, Doxsey SJ, Goldstein LS
JournalJ Cell Biol
PubMed ID9060472
'The kinesin superfamily is a large group of proteins (kinesin-like proteins [KLPs]) that share sequence similarity with the microtubule (MT) motor kinesin. Several members of this superfamily have been implicated in various stages of mitosis and meiosis. Here we report our studies on KLP67A of Drosophila. DNA sequence analysis of ... More
Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment.
AuthorsColvin RA, Laskowski M, Fontaine CP,
JournalBrain Res
PubMed ID16581038
'Zinquin (Zn(2+) selective fluorophore), when used to visualize intracellular Zn(2+), typically shows brightly fluorescent perinuclear endosome-like structures, presumably identifying Zn(2+) containing organelles. In this study, zinquin identified numerous and widespread sites of Zn(2+) compartmentalization in primary cultures of embryonic rat cortical neurons. Nuclear fluorescence, however, was absent. We labeled neuronal ... More
Subcellular localization of Nox4 and regulation in diabetes.
AuthorsBlock K, Gorin Y, Abboud HE,
JournalProc Natl Acad Sci U S A
PubMed ID19706525
'Oxidative stress is implicated in human diseases. Some of the oxidative pathways are harbored in the mitochondria. NAD(P)H oxidases have been identified not only in phagocytic but also in somatic cells. Nox4 is the most ubiquitous of these oxidases and is a major source of reactive oxygen species (ROS) in ... More
Modulation of Mg2+ efflux from rat ventricular myocytes studied with the fluorescent indicator furaptra.
AuthorsTursun P, Tashiro M, Konishi M
JournalBiophys J
PubMed ID15626700
'The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular conditions (0.1 mM EGTA) at 25 degrees C, ... More
Feedback inhibition of sodium/calcium exchange by mitochondrial calcium accumulation.
AuthorsOpuni K, Reeves JP
JournalJ Biol Chem
PubMed ID10801871
'Chinese hamster ovary cells expressing the bovine cardiac Na(+)/Ca(2+) exchanger were subjected to two periods of 5 and 3 min, respectively, during which the extracellular Na(+) concentration ([Na(+)](o)) was reduced to 20 mm; these intervals were separated by a 5-min recovery period at 140 mm Na(+)(o). The cytosolic Ca(2+) concentration ... More
Apparent cytosolic calcium gradients in T-lymphocytes due to fura-2 accumulation in mitochondria.
AuthorsQuintana A, Hoth M
JournalCell Calcium
PubMed ID15193858
'Fura-2 is the most common dye to measure cytosolic Ca2+ concentrations ([Ca2+]i). To facilitate simultaneous imaging of many cells while preserving their cytosolic environment, fura-2 is often loaded into the cytosol in its membrane-permeant ester form. It has been reported that small amounts of fura-2 accumulate in intracellular compartments, an ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
AuthorsNagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
JournalJ Immunol
PubMed ID15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More
MitoTracker labeling in primary neuronal and astrocytic cultures: influence of mitochondrial membrane potential and oxidants.
AuthorsBuckman JF, Hernández H, Kress GJ, Votyakova TV, Pal S, Reynolds IJ
JournalJ Neurosci Methods
PubMed ID11164242
'MitoTracker dyes are fluorescent mitochondrial markers that covalently bind free sulfhydryls. The impact of alterations in mitochondrial membrane potential (Delta Psi(m)) and oxidant stress on MitoTracker staining in mitochondria in cultured neurons and astrocytes has been investigated. p-(Trifluoromethoxy) phenyl-hydrazone (FCCP) significantly decreased MitoTracker loading, except with MitoTracker Green in neurons ... More
Porphyrin-retinamides: synthesis and cellular studies.
AuthorsSibrian-Vazquez M, Jensen TJ, Vicente MG
JournalBioconjug Chem
PubMed ID17518439
'A series of four porphyrin-retinamides containing either all-trans- or 13-cis-retinoid acid residues, directly linked to the para-phenyl position of meso-tetraphenylporphyrin or via a low-molecular-weight PEG spacer, have been synthesized. The biological properties of these conjugates were evaluated in a model cell line, human HEp2, and in neuroblastoma SK-N-DZ cells, which ... More
Cold-induced apoptosis of hepatocytes: mitochondrial permeability transition triggered by nonmitochondrial chelatable iron.
AuthorsRauen U, Kerkweg U, Weisheit D, Petrat F, Sustmann R, de Groot H
JournalFree Radic Biol Med
PubMed ID14680689
'We previously described that the cold-induced apoptosis of cultured hepatocytes is mediated by an increase in the cellular chelatable iron pool. We here set out to assess whether a mitochondrial permeability transition (MPT) is involved in cold-induced apoptosis. When cultured hepatocytes were rewarmed after 18 h of cold (4 degrees ... More
Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.
AuthorsDe Pauw A, Demine S, Tejerina S, Dieu M, Delaive E, Kel A, Renard P, Raes M, Arnould T,
JournalAm J Physiol Endocrinol Metab
PubMed ID22354779
'In adipocytes, mitochondrial uncoupling is known to trigger a triglyceride loss comparable with the one induced by TNFa, a proinflammatory cytokine. However, the impact of a mitochondrial uncoupling on the abundance/composition of mitochondria and its connection with triglyceride content in adipocytes is largely unknown. In this work, the effects of ... More
Ketamine induces toxicity in human neurons differentiated from embryonic stem cells via mitochondrial apoptosis pathway.
AuthorsBosnjak ZJ, Yan Y, Canfield S, Muravyeva MY, Kikuchi C, Wells CW, Corbett JA, Bai X,
JournalCurr Drug Saf
PubMed ID22873495
'Ketamine is widely used for anesthesia in pediatric patients. Growing evidence indicates that ketamine causes neurotoxicity in a variety of developing animal models. Our understanding of anesthesia neurotoxicity in humans is currently limited by difficulties in obtaining neurons and performing developmental toxicity studies in fetal and pediatric populations. It may ... More
Detection of changes in mitochondrial function during apoptosis by simultaneous staining with multiple fluorescent dyes and correlated multiparameter flow cytometry.
AuthorsPoot M, Pierce RH
JournalCytometry
PubMed ID10213196
'BACKGROUND: The possible relationships between changes in mitochondrial membrane potential and other mitochondrial functions during apoptosis remain controversial. METHODS: To detect concomitant changes in mitochondrial function during apoptosis, we performed correlated multiparameter flow cytometry after simultaneous cell staining with several dyes. RESULTS: After camptothecin treatment, nonapoptotic cells exhibited a concomitant ... More
Mitotracker green is a P-glycoprotein substrate.
AuthorsMarques-Santos LF, Oliveira JG, Maia RC, Rumjanek VM
JournalBiosci Rep
PubMed ID14763437
'P-glycoprotein has a widespread expression on normal tissues. The protein has also been strongly associated with the multidrug resistance phenotype (MDR) on tumor cells. The employment of flow cytometry and confocal microscopy has contributed to the discovery and application of new particular fluorescent dyes. Nevertheless, several studies are being performed ... More
Functional genomics in Dictyostelium: MidA, a new conserved protein, is required for mitochondrial function and development.
'Genomic sequencing has revealed a large number of evolutionary conserved genes of unknown function. In the absence of characterized functional domains, the discovery of the role of these genes must rely on experimental approaches. We have selected 30 Dictyostelium discoideum genes of unknown function that showed high similarity to uncharacterized ... More
Mitochondrial permeability transition and swelling can occur reversibly without inducing cell death in intact human cells.
AuthorsMinamikawa T, Williams DA, Bowser DN, Nagley P
JournalExp Cell Res
PubMed ID9882512
'Severe disruption of mitochondrial function is generally considered to provide a powerful trigger for apoptosis in mammalian cells. We report here that intact cells may undergo the mitochondrial permeability transition and mitochondria swell in a fully reversible manner, without inducing cell death. Cultured human osteosarcoma cells (143B TK-) stained with ... More
The mitochondrial membrane potential and Ca2+ oscillations in smooth muscle.
AuthorsChalmers S, McCarron JG,
JournalJ Cell Sci
PubMed ID18073239
'Ca2+ uptake by mitochondria might both modulate the cytosolic Ca2+ concentration ([Ca2+]c) and depolarize the mitochondrial membrane potential (delta Psi m) to limit ATP production. To investigate how physiological Ca2+ signaling might affect energy production, delta Psi m was examined during Ca2+ oscillations in smooth muscle cells. In single, voltage-clamped ... More
A role for mitochondria in NLRP3 inflammasome activation.
AuthorsZhou R, Yazdi AS, Menu P, Tschopp J,
JournalNature
PubMed ID21124315
'An inflammatory response initiated by the NLRP3 inflammasome is triggered by a variety of situations of host ''danger'', including infection and metabolic dysregulation. Previous studies suggested that NLRP3 inflammasome activity is negatively regulated by autophagy and positively regulated by reactive oxygen species (ROS) derived from an uncharacterized organelle. Here we ... More
The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy.
AuthorsLemasters JJ, Nieminen AL, Qian T, Trost LC, Elmore SP, Nishimura Y, Crowe RA, Cascio WE, Bradham CA, Brenner DA, Herman B
JournalBiochim Biophys Acta
PubMed ID9714796
'Using confocal microscopy, onset of the mitochondrial permeability transition (MPT) in individual mitochondria within living cells can be visualized by the redistribution of the cytosolic fluorophore, calcein, into mitochondria. Simultaneously, mitochondria release membrane potential-indicating fluorophores like tetramethylrhodamine methylester. The MPT occurs in several forms of necrotic cell death, including oxidative ... More
Non-uniform distribution of mitochondria in pancreatic acinar cells.
AuthorsJohnson PR, Dolman NJ, Pope M, Vaillant C, Petersen OH, Tepikin AV, Erdemli G
JournalCell Tissue Res
PubMed ID12838407
'The distribution of mitochondria in pancreatic acinar cells was investigated using confocal fluorescence microscopy and transmission electron microscopy (EM). Acinar cells were studied either after enzymatic isolation or in small segments of undisassociated pancreatic tissue. Loading of isolated acinar cells with Mito Tracker Green or Red, a fluorescence mitochondrial probe, ... More
Single-cell analysis by a scanning thermal lens microscope with a microchip: direct monitoring of cytochrome c distribution during apoptosis process.
AuthorsTamaki E, Sato K, Tokeshi M, Sato K, Aihara M, Kitamori T
JournalAnal Chem
PubMed ID12033245
'We developed a microsystem for cell experiments consisting of a scanning thermal lens microscope detection system and a cell culture microchip. The microchip system was good for liquid control in microspace, and this results in secure cell stimulation and coincident in vivo observation of the cell responses. The system could ... More
Pseudoapoptosis induced by brief activation of ATP-gated P2X7 receptors.
AuthorsMackenzie AB, Young MT, Adinolfi E, Surprenant A
JournalJ Biol Chem
PubMed ID15994333
'P2X7 receptors are ATP-gated ion channels primarily expressed on antigen-presenting immune cells where they play a role in the acute inflammatory response. These ion channels couple not only to influx of cations, including calcium, but also to rapid alterations in cell morphology (membrane blebbing, phosphatidylserine exposure, microvesicle shedding). These features ... More
Apoptosis-inducing factor is involved in the regulation of caspase-independent neuronal cell death.
'Caspase-independent death mechanisms have been shown to execute apoptosis in many types of neuronal injury. P53 has been identified as a key regulator of neuronal cell death after acute injury such as DNA damage, ischemia, and excitotoxicity. Here, we demonstrate that p53 can induce neuronal cell death via a caspase-mediated ... More
Mitochondria present in excised patches from pancreatic B-cells may form microcompartments with ATP-dependent potassium channels.
AuthorsRustenbeck I, Dickel C, Herrmann C, Grimmsmann T
JournalBiosci Rep
PubMed ID10888471
'Experiments with inside-out patches excised from pancreatic B-cells have yielded evidence that mitochondria are often contained in the cytoplasmic plug protruding into the tip of patch pipette. When intact B-cells were loaded with the fluorescent mitochondrial stain, rhodamine 123, and membrane patches excised from these cells, a green fluorescence could ... More
Heterogeneity of mitochondrial matrix free ca2+: resolution of Ca2+ dynamics in individual mitochondria in situ.
AuthorsMonteith GR, Blaustein MP
JournalAm J Physiol
PubMed ID10329969
'The role of mitochondria in Ca2+ homeostasis is controversial. We employed the Ca2+-sensitive dye rhod 2 with novel, high temporal and spatial resolution imaging to evaluate changes in the matrix free Ca2+ concentration of individual mitochondria ([Ca2+]m) in agonist-stimulated, primary cultured aortic myocytes. Stimulation with 10 microM serotonin (5-HT) evoked ... More
MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection.
AuthorsPresley AD, Fuller KM, Arriaga EA
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID12880861
'MitoTracker Green (MTG) is a mitochondrial-selective fluorescent label commonly used in confocal microscopy and flow cytometry. It is expected that this dye selectively accumulates in the mitochondrial matrix where it covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues. Here we demonstrate that MTG can ... More
Modulation of [Ca2+]i signaling dynamics and metabolism by perinuclear mitochondria in mouse parotid acinar cells.
AuthorsBruce JI, Giovannucci DR, Blinder G, Shuttleworth TJ, Yule DI
JournalJ Biol Chem
PubMed ID14699167
'Parotid acinar cells exhibit rapid cytosolic calcium signals ([Ca2+]i) that initiate in the apical region but rapidly become global in nature. These characteristic [Ca2+]i signals are important for effective fluid secretion, which critically depends on a synchronized activation of spatially separated ion fluxes. Apically restricted [Ca2+]i signals were never observed ... More
Translocation of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro.
AuthorsSun QY, Wu GM, Lai L, Park KW, Cabot R, Cheong HT, Day BN, Prather RS, Schatten H
JournalReproduction
PubMed ID11425340
'The distribution of active mitochondria during pig oocyte maturation, fertilization and early embryo development in vitro was revealed by using MitoTracker Green staining and confocal laser scanning microscopy. The regulation of mitochondrial translocation by microfilaments and microtubules was also studied. In oocytes collected from small follicles, strong staining of active ... More
Asbestos induces mitochondrial DNA damage and dysfunction linked to the development of apoptosis.
AuthorsShukla A, Jung M, Stern M, Fukagawa NK, Taatjes DJ, Sawyer D, Van Houten B, Mossman BT
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12909582
'To test the hypothesis that asbestos-mediated cell injury is mediated through an oxidant-dependent mitochondrial pathway, isolated mesothelial cells were examined for mitochondrial DNA damage as determined by quantitative PCR. Mitochondrial DNA damage occurred at fourfold lower concentrations of crocidolite asbestos compared with concentrations required for nuclear DNA damage. DNA damage ... More
Phosphoaspirin (MDC-43), a novel benzyl ester of aspirin, inhibits the growth of human cancer cell lines more potently than aspirin: a redox-dependent effect.
'Aspirin is chemopreventive against colon and probably other cancers, but this effect is relatively weak and its chronic administration to humans is associated with significant side effects. Because of these limitations, extensive effort has been exerted to improve the pharmacological properties of aspirin. We have determined the anticancer activity and ... More
Bax regulates production of superoxide in both apoptotic and nonapoptotic neurons: role of caspases.
AuthorsKirkland RA, Saavedra GM, Cummings BS, Franklin JL,
JournalJ Neurosci
PubMed ID21123558
'A Bax- and, apparently, mitochondria-dependent increase in superoxide (O(2)(·-)) and other reactive oxygen species (ROS) occurs in apoptotic superior cervical ganglion (SCG) and cerebellar granule (CG) neurons. Here we show that Bax also lies upstream of ROS produced in nonapoptotic neurons and present evidence that caspases partially mediate the pro-oxidant ... More
Suppression of mitochondria-dependent neutrophil apoptosis with thermal injury.
AuthorsHu Z, Sayeed MM
JournalAm J Physiol Cell Physiol
PubMed ID13679304
'Neutrophil apoptosis is delayed under trauma and/or sepsis conditions. The mechanism for the delay has remained unclear. We hypothesize that modulation of the mitochondrial pathway of apoptosis contributes to the delay in neutrophil apoptosis with burn injury. Rats were subjected to burn injury (30% of total body surface area, 98 ... More
Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells.
AuthorsHodge S, Hodge G, Scicchitano R, Reynolds PN, Holmes M
JournalImmunol Cell Biol
PubMed ID12848850
'Chronic obstructive pulmonary disease is a highly prevalent, complex disease, usually caused by cigarette smoke. It causes serious morbidity and mortality and costs the global community billions of dollars per year. While chronic inflammation, extracellular matrix destruction and increased airway epithelial cell apoptosis are reported in chronic obstructive pulmonary disease, ... More
Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro.
AuthorsMukhopadhyay P, Rajesh M, Bátkai S, Kashiwaya Y, Haskó G, Liaudet L, Szabó C, Pacher P,
JournalAm J Physiol Heart Circ Physiol
PubMed ID19286953
'Doxorubicin (DOX) is a potent available antitumor agent; however, its clinical use is limited because of its cardiotoxicity. Cell death is a key component in DOX-induced cardiotoxicity, but its mechanisms are elusive. Here, we explore the role of superoxide, nitric oxide (NO), and peroxynitrite in DOX-induced cell death using both ... More
Active mitochondria surrounding the pancreatic acinar granule region prevent spreading of inositol trisphosphate-evoked local cytosolic Ca(2+) signals.
'Agonist-evoked cytosolic Ca(2+) spikes in mouse pancreatic acinar cells are specifically initiated in the apical secretory pole and are mostly confined to this region. The role played by mitochondria in this process has been investigated. Using the mitochondria-specific fluorescent dyes MitoTracker Green and Rhodamine 123, these organelles appeared as a ... More
Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes.
AuthorsBa X, Gupta S, Davidson M, Garg NJ,
JournalJ Biol Chem
PubMed ID20145242
'In this study, we demonstrate that human cardiomyocytes (AC16) produce reactive oxygen species (ROS) and inflammatory cytokines in response to Trypanosoma cruzi. ROS were primarily produced by mitochondria, some of which diffused to cytosol of infected cardiomyocytes. These ROS resulted in an increase in 8-hydroxyguanine lesions and DNA fragmentation that ... More
Subcellular positioning of small molecules.
AuthorsTakayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM
JournalNature
PubMed ID11429594
Sustained activity of calcium release-activated calcium channels requires translocation of mitochondria to the plasma membrane.
AuthorsQuintana A, Schwarz EC, Schwindling C, Lipp P, Kaestner L, Hoth M
JournalJ Biol Chem
PubMed ID17056596
'A rise of the intracellular Ca(2+) concentration has multiple signaling functions. Sustained Ca(2+) influx across plasma membrane through calcium release-activated calcium (CRAC) channels is required for T-cell development in the thymus, gene transcription, and proliferation and differentiation of naïve T-cells into armed effectors cells. Intracellular Ca(2+) signals are shaped by ... More
Granzyme B induces BID-mediated cytochrome c release and mitochondrial permeability transition.
AuthorsAlimonti JB, Shi L, Baijal PK, Greenberg AH
JournalJ Biol Chem
PubMed ID11114298
'Many cell death pathways converge at the mitochondria to induce release of apoptogenic proteins and permeability transition, resulting in the activation of effector caspases responsible for the biochemical and morphological alterations of apoptosis. The death receptor pathway has been described as a triphasic process initiated by the activation of apical ... More
Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.
AuthorsBannai H, Inoue T, Nakayama T, Hattori M, Mikoshiba K
JournalJ Cell Sci
PubMed ID14676272
'Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal ... More
Activation of serine/threonine protein phosphatase-1 is required for ceramide-induced survival of sympathetic neurons.
AuthorsPlummer G, Perreault KR, Holmes CF, Posse De Chaves EI
JournalBiochem J
PubMed ID15361069
'In sympathetic neurons, C6-ceramide, as well as endogenous ceramides, blocks apoptosis elicited by NGF (nerve growth factor) deprivation. The mechanism(s) involved in ceramide-induced neuronal survival are poorly understood. Few direct targets for the diverse cellular effects of ceramide have been identified. Amongst those proposed is PP-1c, the catalytic subunit of ... More
Galectin-1 sensitizes resting human T lymphocytes to Fas (CD95)-mediated cell death via mitochondrial hyperpolarization, budding, and fission.
AuthorsMatarrese P, Tinari A, Mormone E, Bianco GA, Toscano MA, Ascione B, Rabinovich GA, Malorni W
JournalJ Biol Chem
PubMed ID15556941
'Galectins have emerged as a novel family of immunoregulatory proteins implicated in T cell homeostasis. Recent studies showed that galectin-1 (Gal-1) plays a key role in tumor-immune escape by killing antitumor effector T cells. Here we found that Gal-1 sensitizes human resting T cells to Fas (CD95)/caspase-8-mediated cell death. Furthermore, ... More
Evidence for secretory pathway localization of a voltage-dependent anion channel isoform.
AuthorsBuettner R, Papoutsoglou G, Scemes E, Spray DC, Dermietzel R
JournalProc Natl Acad Sci U S A
PubMed ID10716730
'Voltage-dependent anion channels (VDACs) are pore-forming proteins (porins) that form the major pathway for movement of adenine nucleotides through the outer mitochondrial membrane. Electrophysiological studies indicate that VDAC-like channel activity is also prevalent in the cell membranes of many mammalian cells. However, the multitopological localization of porins outside the mitochondrion ... More
Fluorescence correlation microscopy of cells in the presence of autofluorescence.
AuthorsBrock R, Hink MA, Jovin TM
JournalBiophys J
PubMed ID9788950
'Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes with mitochondria, ... More
Role of mitochondria in calcium regulation of spontaneously contracting cardiac muscle cells.
AuthorsBowser DN, Minamikawa T, Nagley P, Williams DA
JournalBiophys J
PubMed ID9746542
'Mitochondrial involvement in the regulation of cytosolic calcium concentration ([Ca2+]i) in cardiac myocytes has been largely discounted by many authors. However, recent evidence, including the results of this study, has forced a reappraisal of this role. [Ca2+]i and Ca2+ in the mitochondria ([Ca2+]m) were measured in this study with specific ... More
Rapid activation of antioxidant defenses by nerve growth factor suppresses reactive oxygen species during neuronal apoptosis: evidence for a role in cytochrome c redistribution.
AuthorsKirkland RA, Saavedra GM, Franklin JL,
JournalJ Neurosci
PubMed ID17942726
'Depriving mouse sympathetic neurons of nerve growth factor (NGF) causes their apoptotic death. A Bax-dependent increase of mitochondrial-derived reactive oxygen species (ROS) begins in these cells soon after NGF withdrawal. We investigated the effects on these ROS of adding NGF to cultures of NGF-deprived neurons. ROS levels were monitored with ... More
Mitochondria play no roles in Mn(II)-induced apoptosis in HeLa cells.
AuthorsOubrahim H, Stadtman ER, Chock PB
JournalProc Natl Acad Sci U S A
PubMed ID11493712
'Manganese(II) has been shown to exhibit catalase-like activity under physiological conditions. In the course of studies to test the antioxidant activity of Mn(II) on HeLa cells, it was observed at high concentrations (1-2 mM) that Mn(II) also induced apoptosis, as judged by changes in cell morphology, caspase-3 activation, cleavage of ... More
Characteristics of intermittent mitochondrial transport in guinea pig enteric nerve fibers.
AuthorsVanden Berghe P, Hennig GW, Smith TK
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID14592946
'Enteric neurons controlling various gut functions are prone to oxidative insults that might damage mitochondria (e.g., intestinal inflammation). To resume local energy supply, mitochondria need to be transported. We used MitoTracker dyes and confocal microscopy to investigate basic characteristics of mitochondrial transport in guinea pig myenteric neurites. During a 10-s ... More
Agonist-evoked mitochondrial Ca2+ signals in mouse pancreatic acinar cells.
AuthorsGonzález A, Schulz I, Schmid A
JournalJ Biol Chem
PubMed ID10995756
'In the present study we have investigated cytosolic and mitochondrial Ca(2+) signals in isolated mouse pancreatic acinar cells double-loaded with the fluorescent probes fluo-3 and rhod-2. Stimulation of pancreatic acinar cells with 500 nm acetylcholine caused release of Ca(2+) from intracellular stores and produced cytosolic Ca(2+) signals in form of ... More
Intracellular localization of the radiation enhancer motexafin gadolinium using interferometric Fourier fluorescence microscopy.
AuthorsWoodburn KW
JournalJ Pharmacol Exp Ther
PubMed ID11356908
'Motexafin gadolinium (MGd) is a unique therapeutic agent that localizes in cancer cells and increases tumor response to ionizing radiation and certain chemotherapeutics. The in vitro intracellular localization, accumulation, and retention of MGd in murine EMT6 mammary sarcoma and Rif-1 fibrosarcoma cell lines were studied using interferometric Fourier fluorescence microscopy. ... More
Mitochondrial PO2 measured by delayed fluorescence of endogenous protoporphyrin IX.
AuthorsMik EG, Stap J, Sinaasappel M, Beek JF, Aten JA, van Leeuwen TG, Ince C
JournalNat Methods
PubMed ID17060918
'Molecular oxygen is the primary oxidant in biological systems. The ultimate destination of oxygen in vivo is the mitochondria where it is used in oxidative phosphorylation. The ability of this process to produce an amount of high-energy phosphates adequate to sustain life highly depends on the available amount of oxygen. ... More
Differential effects of superoxide dismutase isoform expression on hydroperoxide-induced apoptosis in PC-12 cells.
AuthorsPias EK, Ekshyyan OY, Rhoads CA, Fuseler J, Harrison L, Aw TY
JournalJ Biol Chem
PubMed ID12551919
'The current study examines the contribution of mitochondria-derived reactive oxygen species (ROS) in tert-butyl-hydroperoxide (TBH)-induced apoptotic signaling using clones of undifferentiated pheochromocytoma (PC-12) cells that stably overexpress the human mitochondrial or cytoplasmic forms of superoxide dismutase (SOD) (viz. Mn-SOD or CuZn-SOD, respectively). Exposure of wild type cells to TBH caused ... More
Origin sites of calcium release and calcium oscillations in frog sympathetic neurons.
AuthorsMcDonough SI, Cseresnyés Z, Schneider MF
JournalJ Neurosci
PubMed ID11124983
'In many neurons, Ca(2+) signaling depends on efflux of Ca(2+) from intracellular stores into the cytoplasm via caffeine-sensitive ryanodine receptors (RyRs) of the endoplasmic reticulum. We have used high-speed confocal microscopy to image depolarization- and caffeine-evoked increases in cytoplasmic Ca(2+) levels in individual cultured frog sympathetic neurons. Although caffeine-evoked Ca(2+) ... More
Effect of transient and permanent permeability transition pore opening on NAD(P)H localization in intact cells.
'To study the effect of mitochondrial permeability transition pore (PTP) opening on NAD(P)H localization, intact cells were exposed to the Ca(2+) ionophore A23187. PTP opening, mitochondrial membrane potential, mitochondrial volume, and NAD(P)H localization were assessed by time-lapse laser confocal microscopy using the calcein-cobalt technique, tetramethylrhodamine methyl ester, MitoTracker, and NAD(P)H ... More
A mitochondrion-specific dye for multicolour fluorescent imaging of Trypanosoma brucei.
AuthorsVassella E, Straesser K, Boshart M
JournalMol Biochem Parasitol
PubMed ID9497065
Probing mitochondrial probes.
AuthorsIsola R, Falchi AM, Diana A, Diaz G
JournalCytometry
PubMed ID11002271
Monitoring simultaneous subcellular events in vitro by means of coherent multiprobe fluorescence.
AuthorsPlymale DR, Haskins JR, de la Iglesia FA
JournalNat Med
PubMed ID10086396
Real-time imaging of astrocyte response to quantum dots: in vivo screening model system for biocompatibility of nanoparticles.
AuthorsMaysinger D, Behrendt M, Lalancette-Hébert M, Kriz J
JournalNano Lett
PubMed ID17638392
Astrocytes are the principle macroglial brain cells. They are activated by different stressors and brain injuries. Quantum dots (QDs) can cause oxidative stress. This study shows a real-time imaging of primary cortical cultures and assessment of QD-induced activation of astrocytes in the brains of transgenic mice with the luciferase gene ... More
Lack of acrosome formation in Hrb-deficient mice.
AuthorsKang-Decker N, Mantchev GT, Juneja SC, McNiven MA, van Deursen JM
JournalScience
PubMed ID11711676
The sperm acrosome is essential for sperm-egg fusion and is often defective in men with nonobstructive infertility. Here we report that male mice with a null mutation in Hrb are infertile and display round-headed spermatozoa that lack an acrosome. In wild-type spermatids, Hrb is associated with the cytosolic surface of ... More
Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin.
AuthorsHollinshead M, Sanderson J, Vaux DJ
JournalJ Histochem Cytochem
PubMed ID9267466
The mitochondrial matrix contains endogenously biotinylated proteins. These proteins can cause unexpected background signal when biotin-avidin- or biotin-streptavidin-based detection systems are used in immunocytochemistry. Here we show that this reactivity can be deliberately exploited, using a simple anti-biotin reagent, to obtain strong and highly specific labeling of mitochondria by both ... More
Mitochondrial biogenesis in mammals: the role of endogenous nitric oxide.
AuthorsNisoli E, Clementi E, Paolucci C, Cozzi V, Tonello C, Sciorati C, Bracale R, Valerio A, Francolini M, Moncada S, Carruba MO
JournalScience
PubMed ID12574632
Nitric oxide was found to trigger mitochondrial biogenesis in cells as diverse as brown adipocytes and 3T3-L1, U937, and HeLa cells. This effect of nitric oxide was dependent on guanosine 3',5'-monophosphate (cGMP) and was mediated by the induction of peroxisome proliferator-activated receptor gamma coactivator 1alpha, a master regulator of mitochondrial ... More
Fluorescent histochemical techniques for analysis of intracellular signaling.
AuthorsOksvold MP, Skarpen E, Widerberg J, Huitfeldt HS
JournalJ Histochem Cytochem
PubMed ID11850432
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques ... More
Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice.
AuthorsHildebrand JD, Soriano P
JournalCell
PubMed ID10589677
Using gene trap mutagenesis, we have identified a mutation in mice that causes exencephaly, acrania, facial clefting, and spina bifida, all of which can be attributed to failed neural tube closure. This mutation is designated shroom (shrm) because the neural folds "mushroom" outward and do not converge at the dorsal ... More
T cell activation requires mitochondrial translocation to the immunological synapse.
AuthorsQuintana A, Schwindling C, Wenning AS, Becherer U, Rettig J, Schwarz EC, Hoth M,
JournalProc Natl Acad Sci U S A
PubMed ID17726106
T helper (Th) cell activation is required for the adaptive immune response. Formation of the immunological synapse (IS) between Th cells and antigen-presenting cells is essential for Th cell activation. IS formation induces the polarization and redistribution of many signaling molecules; however, very little is known about organelle redistribution during ... More
Identification of a protective role for protein phosphatase 1cgamma1 against oxidative stress-induced vascular smooth muscle cell apoptosis.
AuthorsTchivilev I, Madamanchi NR, Vendrov AE, Niu XL, Runge MS,
JournalJ Biol Chem
PubMed ID18540044
The development of therapeutic strategies to inhibit reactive oxygen species (ROS)-mediated damage in blood vessels has been limited by a lack of specific targets for intervention. Targeting ROS-mediated events in the vessel wall is of interest, because ROS play important roles throughout atherogenesis. In early atherosclerosis, ROS stimulate vascular smooth ... More
Manganese superoxide dismutase induces p53-dependent senescence in colorectal cancer cells.
AuthorsBehrend L, Mohr A, Dick T, Zwacka RM,
JournalMol Cell Biol
PubMed ID16107721
The mitochondrial enzyme manganese superoxide dismutase (MnSOD) is known to suppress cell growth in different tumor cell lines. However, the molecular mechanism of this growth-retarding effect is not fully understood. Here we show that overexpression of MnSOD slows down growth of HCT116 human colorectal cancer cells by induction of cellular ... More
Different molecular mechanisms involved in spontaneous and oxidative stress-induced mitochondrial fragmentation in TriPeptidyl Peptidase-1 (TPP-1) deficient fibroblasts.
AuthorsVan Beersel G, Tihon E, Demine S, Hamer I, Jadot M, Arnould T,
JournalBiosci Rep
PubMed ID23249249
The Neuronal Ceroid Lipofuscinosis (NCLs) form a group of 8 inherited autosomal recessive diseases characterized by the intralysosomal accumulation of autofluorescent pigments, called ceroids. Recent data suggest that the pathogenesis of NCLs is associated with the appearance of fragmented mitochondria with altered functions. However, even if an impairement in the ... More
Apoptosis-inducing factor (AIF) is targeted in IFN-a2a-induced Bid-mediated apoptosis through Bak activation in ovarian cancer cells.
AuthorsMiyake K, Bekisz J, Zhao T, Clark CR, Zoon KC,
JournalBiochim Biophys Acta
PubMed ID22683989
Previously we have shown that interferon (IFN)-a induced apoptosis is predominantly mediated by the upregulation of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) via the caspase-8 pathway. It was also shown that recruitment of mitochondria in IFN-a induced apoptosis involves the cleavage of BH3 interacting domain death agonist (Bid) to ... More
Photoinduced cytotoxicity and biodistribution of prostate cancer cell-targeted porphyrins.
AuthorsSehgal I, Sibrian-Vazquez M, Vicente MG,
JournalJ Med Chem
PubMed ID18839477
A series of five porphyrin-peptide conjugates bearing one or two sequences containing a cell penetrating peptide (CPP), a nuclear localization signal (NLS), or a bifunctional CPP-NLS or NLS-CPP sequences were synthesized and investigated in vitro using PC-3M human prostate cancer cells, in comparison with FDA-approved purified hematoporphyrin derivative (Porfimer Sodium) ... More
Interleukin-6 counteracts therapy-induced cellular oxidative stress in multiple myeloma by up-regulating manganese superoxide dismutase.
AuthorsBrown CO, Salem K, Wagner BA, Bera S, Singh N, Tiwari A, Choudhury A, Buettner GR, Goel A,
JournalBiochem J
PubMed ID22471522
IL (interleukin)-6, an established growth factor for multiple myeloma cells, induces myeloma therapy resistance, but the resistance mechanisms remain unclear. The present study determines the role of IL-6 in re-establishing intracellular redox homoeostasis in the context of myeloma therapy. IL-6 treatment increased myeloma cell resistance to agents that induce oxidative ... More
Hyperspectral image analysis of live cells in various cell cycle stages.
AuthorsDicker DT, Lerner JM, El-Deiry WS,
JournalCell Cycle
PubMed ID17912031
In this study we have explored the use of hyperspectral imaging (HSI) to determine the cell cycle status of live cells in culture. Live cancer cell lines in culture were either synchronized by release from nocodazole or arrested in various cell-cycle phases with serum starvation (G1), aphidicolin (S), or nocodazole ... More
Determining the distribution of probes between different subcellular locations through automated unmixing of subcellular patterns.
Many proteins or other biological macromolecules are localized to more than one subcellular structure. The fraction of a protein in different cellular compartments is often measured by colocalization with organelle-specific fluorescent markers, requiring availability of fluorescent probes for each compartment and acquisition of images for each in conjunction with the ... More
Dynamics and mechanisms of quantum dot nanoparticle cellular uptake.
AuthorsXiao Y, Forry SP, Gao X, Holbrook RD, Telford WG, Tona A,
JournalJ Nanobiotechnology
PubMed ID20550705
ABSTRACT: BACKGROUND: The rapid growth of the nanotechnology industry and the wide application of various nanomaterials have raised concerns over their impact on the environment and human health. Yet little is known about the mechanism of cellular uptake and cytotoxicity of nanoparticles. An array of nanomaterials has recently been introduced ... More
Evidence that the SpoIIIE DNA translocase participates in membrane fusion during cytokinesis and engulfment.
AuthorsLiu NJ, Dutton RJ, Pogliano K,
JournalMol Microbiol
PubMed ID16430687
During Bacillus subtilis sporulation, SpoIIIE is required for translocation of the trapped forespore chromosome across the sporulation septum, for compartmentalization of cell-specific gene expression, and for membrane fusion after engulfment. We isolated mutations within the SpoIIIE membrane domain that block localization and function. One mutant protein initially localizes normally and ... More
Fluorescence staining of live cyanobacterial cells suggest non-stringent chromosome segregation and absence of a connection between cytoplasmic and thylakoid membranes.
AuthorsSchneider D, Fuhrmann E, Scholz I, Hess WR, Graumann PL,
JournalBMC Cell Biol
PubMed ID17767716
BACKGROUND: In spite of their abundance and importance, little is known about cyanobacterial cell biology and their cell cycle. During each cell cycle, chromosomes must be separated into future daughter cells, i.e. into both cell halves, which in many bacteria is achieved by an active machinery that operates during DNA ... More
Autophagy in neurite injury and neurodegeneration: in vitro and in vivo models.
Recent advances indicate that maintaining a balanced level of autophagy is critically important for neuronal health and function. Pathologic dysregulation of macroautophagy has been implicated in synaptic dysfunction, cellular stress, and neuronal cell death. Autophagosomes and autolysosomes are induced in acute and chronic neurological disorders including stroke, brain trauma, neurotoxin ... More
Caspase-cleaved tau expression induces mitochondrial dysfunction in immortalized cortical neurons: implications for the pathogenesis of Alzheimer disease.
AuthorsQuintanilla RA, Matthews-Roberson TA, Dolan PJ, Johnson GV,
JournalJ Biol Chem
PubMed ID19389700
In Alzheimer disease (AD) mitochondrial abnormalities occur early in the pathogenic process and likely play a significant role in disease progression. Tau is a microtubule-associated protein that is abnormally processed in AD, and a connection between tau pathology and mitochondrial impairment has been proposed. However, few studies have examined the ... More
The utilization of pathogen-like cellular trafficking by single chain block copolymer.
Amphiphilic triblock copolymer, poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), Pluronic P85, is unexpectedly shown to utilize sophisticated cellular trafficking mechanisms and enter brain microvessel endothelial cells and primary neurons that are poorly penetrable. Though caveolae serve as a primary entry site for the copolymer single chains, in cells devoid of caveolae, the ... More
Rosiglitazone treatment prevents mitochondrial dysfunction in mutant huntingtin-expressing cells: possible role of peroxisome proliferator-activated receptor-gamma (PPARgamma) in the pathogenesis of Huntington disease.
AuthorsQuintanilla RA, Jin YN, Fuenzalida K, Bronfman M, Johnson GV,
JournalJ Biol Chem
PubMed ID18640979
Peroxisome proliferator-activated receptor-gamma (PPARgamma) is a member of the PPAR family of transcription factors. Synthetic PPARgamma agonists are used as oral anti-hyperglycemic drugs for the treatment of non-insulin-dependent diabetes. However, emerging evidence indicates that PPARgamma activators can also prevent or attenuate neurodegeneration. Given these previous findings, the focus of this ... More
Metformin and phenethyl isothiocyanate combined treatment in vitro is cytotoxic to ovarian cancer cultures.
AuthorsChan DK, Miskimins WK,
JournalJ Ovarian Res
PubMed ID22781119
High mortality rates in ovarian cancer are largely a result of resistance to currently used chemotherapies. Expanding therapies with a variety of drugs has the potential to reduce this high mortality rate. Metformin and phenethyl isothiocyanate (PEITC) are both potentially useful in ovarian cancer, and they are particularly attractive because ... More
Mitochondria supply membranes for autophagosome biogenesis during starvation.
Starvation-induced autophagosomes engulf cytosol and/or organelles and deliver them to lysosomes for degradation, thereby resupplying depleted nutrients. Despite advances in understanding the molecular basis of this process, the membrane origin of autophagosomes remains unclear. Here, we demonstrate that, in starved cells, the outer membrane of mitochondria participates in autophagosome biogenesis. ... More
pLG72 modulates intracellular D-serine levels through its interaction with D-amino acid oxidase: effect on schizophrenia susceptibility.
AuthorsSacchi S, Bernasconi M, Martineau M, Mothet JP, Ruzzene M, Pilone MS, Pollegioni L, Molla G,
JournalJ Biol Chem
PubMed ID18544534
Human genes coding for pLG72 and d-amino acid oxidase have recently been linked to the onset of schizophrenia. pLG72 was proposed as an activator of the human FAD-containing flavoprotein d-amino acid oxidase (hDAAO). In the brain this oxidizes d-serine, a potent activator of N-methyl-d-aspartate receptor. We have investigated the mechanistic ... More
Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
AuthorsMortensen M, Ferguson DJ, Edelmann M, Kessler B, Morten KJ, Komatsu M, Simon AK,
JournalProc Natl Acad Sci U S A
PubMed ID20080761
Timely elimination of damaged mitochondria is essential to protect cells from the potential harm of disordered mitochondrial metabolism and release of proapoptotic proteins. In mammalian red blood cells, the expulsion of the nucleus followed by the removal of other organelles, such as mitochondria, are necessary differentiation steps. Mitochondrial sequestration by ... More
Enzyme-assisted photosensitization with rose Bengal acetate induces structural and functional alteration of mitochondria in HeLa cells.
Rose Bengal acetate (RB-Ac) can be used as a fluorogenic substrate for photosensitization of cells both in vivo and in vitro: once inside the cells, RB-Ac is converted into photoactive rose Bengal (RB) molecules which redistribute dynamically in the cytoplasm and, upon irradiation by visible green light, can damage organelles ... More
Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing.
AuthorsQian Y, Banerjee S, Grossman CE, Amidon W, Nagy G, Barcza M, Niland B, Karp DR, Middleton FA, Banki K, Perl A,
JournalBiochem J
PubMed ID18498245
TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete ... More
Selective chemical treatment of cellular microdomains using multiple laminar streams.
AuthorsTakayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM
JournalChem Biol
PubMed ID12618184
There are many experiments in which it would be useful to treat a part of the surface or interior of a cell with a biochemical reagent. It is difficult, however, to achieve subcellular specificity, because small molecules diffuse distances equal to the extent of the cell in seconds. This paper ... More
Tumor necrosis factor induces hyperphosphorylation of kinesin light chain and inhibits kinesin-mediated transport of mitochondria.
AuthorsDe Vos K, Severin F, Van Herreweghe F, Vancompernolle K, Goossens V, Hyman A, Grooten J
JournalJ Cell Biol
PubMed ID10851018
The molecular motor kinesin is an ATPase that mediates plus end-directed transport of organelles along microtubules. Although the biochemical properties of kinesin are extensively studied, conclusive data on regulation of kinesin-mediated transport are largely lacking. Previously, we showed that the proinflammatory cytokine tumor necrosis factor induces perinuclear clustering of mitochondria. ... More
Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stress.
AuthorsStamer K, Vogel R, Thies E, Mandelkow E, Mandelkow EM
JournalJ Cell Biol
PubMed ID11901170
We studied the effect of microtubule-associated tau protein on trafficking of vesicles and organelles in primary cortical neurons, retinal ganglion cells, and neuroblastoma cells. Tau inhibits kinesin-dependent transport of peroxisomes, neurofilaments, and Golgi-derived vesicles into neurites. Loss of peroxisomes makes cells vulnerable to oxidative stress and leads to degeneration. In ... More