Endocytic clathrin-coated pit formation is independent of receptor internalization signal levels.
AuthorsSantini F, Marks MS, Keen JH
JournalMol Biol Cell
PubMed ID9571248
'The mechanisms responsible for coated pit formation in cells remain unknown, but indirect evidence has argued both for and against a critical role of receptor cytoplasmic domains in the process. If the endocytic motifs of receptors are responsible for recruiting AP2 to the plasma membrane, thereby driving coated pit formation, ... More
Volume measurements in confocal microscopy.
AuthorsSieck GC, Mantilla CB, Prakash YS
JournalMethods Enzymol
PubMed ID10506980
The polarized sorting of membrane proteins expressed in cultured hippocampal neurons using viral vectors.
AuthorsJareb M, Banker G
JournalNeuron
PubMed ID9620691
One model of neuronal polarity (Dotti and Simons, 1990) proposes that neurons and polarized epithelia use similar mechanisms to sort membrane proteins. To explore this hypothesis, we used viral vectors to express proteins in cultured neurons and assessed their distribution using quantitative immunofluorescence microscopy. Basolateral epithelial proteins were polarized to ... More
The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.
The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The ... More
Microvascular basal lamina antigens disappear during cerebral ischemia and reperfusion.
AuthorsHamann GF, Okada Y, Fitridge R, del Zoppo GJ
JournalStroke
PubMed ID7482660
BACKGROUND AND PURPOSE: Changes in vascular permeability are well-known and important consequences of cerebral ischemia. The development of edema and of petechial hemorrhage is connected to altered vascular integrity. A major part in microvascular integrity is played by the basal lamina. METHODS: The fates of the basal lamina components laminin, ... More
JNK activation and translocation to mitochondria mediates mitochondrial dysfunction and cell death induced by VDAC opening and sorafenib in hepatocarcinoma cells.
AuthorsHeslop KA, Rovini A, Hunt EG, Fang D, Morris ME, Christie CF, Gooz MB, DeHart DN, Dang Y, Lemasters JJ, Maldonado EN
JournalBiochem Pharmacol
PubMed ID31759978
'The multikinase inhibitor sorafenib, and opening of voltage dependent anion channels (VDAC) by the erastin-like compound X1 promotes oxidative stress and mitochondrial dysfunction in hepatocarcinoma cells. Here, we hypothesized that X1 and sorafenib induce mitochondrial dysfunction by increasing reactive oxygen species (ROS) formation and activating c-Jun N-terminal kinases (JNKs), leading ... More
Characterizing the three-dimensional organization of telomeres in papillary thyroid carcinoma cells.
AuthorsCaria P, Dettori T, Frau DV, Lichtenzstejn D, Pani F, Vanni R, Mai S
JournalJ Cell Physiol
PubMed ID30328617
The relationship between the three-dimensional (3D) nuclear telomere architecture and specific genetic alterations in papillary thyroid carcinoma (PTC), in particular in cancer stem-like cells (CSLCs), has not yet been investigated. We isolated thyrospheres containing CSLCs from B-CPAP, K1, and TPC-1 PTC-derived cell lines, representative of tumors with different genetic backgrounds ... More
Statin-dependent modulation of mitochondrial metabolism in cancer cells is independent of cholesterol content.
AuthorsChristie CF, Fang D, Hunt EG, Morris ME, Rovini A, Heslop KA, Beeson GC, Beeson CC, Maldonado EN
JournalFASEB J
PubMed ID30951369
Statins, widely used to treat hypercholesterolemia, inhibit the 3-hydroxy-3-methylglutaryl-coenzyme A reductase, the rate-limiting enzyme of