'Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, ... More
Chemiluminescent probes for singlet oxygen in biological reactions.
AuthorsThompson A, Seliger HH, Posner GH
JournalMethods Enzymol
PubMed ID3821552
Alkylation of enolate ions generated regiospecifically via organocopper reactions. Synthesis of decalin sesquiterpene valerane and of prostaglandin model systems.
AuthorsPosner GH, Sterling JJ, Whitten CE, Lentz CM, Brunelle DJ
JournalJ Am Chem Soc
PubMed ID1133327
A chemiluminescent probe specific for singlet oxygen.
AuthorsPosner GH, Lever JR, Miura K, Lisek C, Seliger HH, Thompson A
JournalBiochem Biophys Res Commun
PubMed ID6435621
We have synthesized a methoxyvinylpyrene (MVP) in order to model the mechanism for the observed microsomal chemiluminescence of benzo[a]pyrene 7,8-dihydrodiol, the proximate carcinogenic metabolite of benzo[a]pyrene. This MVP analog has been found to be a highly efficient and specific chemiluminescent probe for picomole quantities of singlet oxygen and singlet oxygen ... More
Microsomal chemiluminescence of benzo[a]pyrene-7,8-dihydrodiol and its synthetic analogues trans- and cis-1-methoxyvinylpyrene.
AuthorsThompson A, Biggley WH, Posner GH, Lever JR, Seliger HH
JournalBiochim Biophys Acta
PubMed ID3708001
The cis- and trans-methoxyvinylpyrene (MVP) analogues of benzo[a]pyrene-7,8-dihydrodiol (7,8-diol) produce specific microsomal chemiluminescence comparable to that produced from 7,8-diol in Aroclor-induced rat liver microsome preparations. The chemiluminescence quantum yields, emission spectra, and the concentration and the temporal kinetics of these three substrates have been examined. Radiolabelled 7,8-diol and t-MVP exhibit ... More
Nitric oxide enhances the manganese superoxide dismutase-dependent suppression of proliferation in HT-1080 fibrosarcoma cells.
AuthorsMelendez JA, Melathe RP, Rodriguez AM, Mazurkiewicz JE, Davies KJ
JournalCell Growth Differ
PubMed ID10511315
The overexpression of manganese superoxide dismutase (MnSOD), an enzyme that catalyzes the removal of superoxide (O2*-) from the mitochondria, has been shown to be closely associated with tumor regression in vivo and loss of the malignant phenotype in vitro. To investigate the mechanism by which MnSOD overexpression mediates this reversal, ... More